AUTHOR=Brambilla Cristina , Moscatelli Nicol , Lanzani Valentina , Molinari Tosatti Lorenzo , Brusaferri Alessandro , Scano Alessandro 

TITLE=The trade-off between maximizing reconstruction and physiological interpretation of muscle synergies with autoencoders

JOURNAL=Frontiers in Human Neuroscience

VOLUME=Volume 19 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/human-neuroscience/articles/10.3389/fnhum.2025.1699799

DOI=10.3389/fnhum.2025.1699799

ISSN=1662-5161

ABSTRACT=IntroductionIn neuroscience, the muscle synergy method is a widely known computational approach for studying motor control from electromyographic (EMG) recordings. Standard algorithms for synergy extraction rely on a linearity assumption for synergy combination. However, the interactions between muscle groups and movement dynamics often exhibit non-linear characteristics, suggesting the need for alternative approaches. In this context, autoencoders (AEs) have been proposed as promising tools. However, previous studies focused on the reconstruction accuracy optimization and not on the structure of the synergies, and the influence of AE design parameters has not been thoroughly investigated. This study aims to explore the impact of different activation functions on the effectiveness of AEs.MethodsTo this end, we used a rich dataset of upper-limb EMG signals recorded from 16 muscles in 15 participants performing reaching movements toward 9 targets across 5 planes. We evaluated the effects of combining four activation functions in the encoder and decoder layers—linear, ReLU, sigmoid, and tanh—and compared to standard non-negative matrix factorization (NMF).ResultsOur findings show that the extracted synergies are highly sensitive to the AE architecture. Notably, the configurations obtaining the best signal reconstruction do not correspond to the most physiologically meaningful synergies, which were instead achieved with the ReLU+tanh configuration.DiscussionThis suggests that optimizing reconstruction accuracy may result in non-interpretable synergy structures. This research emphasizes the role of non-linear techniques in extracting muscle synergy from different datasets (e.g., lower limbs, full-body movements, patient populations) and identifies the optimal combination of transfer functions for the encoder and decoder layers.