AUTHOR=Lin Jie , Lei Xuemei , Li Yanhong , Jiang Xin , Jiang Fengle , Guo Aihua , Cai Xintong , Ye Xingming , Sun Yang 

TITLE=Development of a senescence-related lncRNA signature in endometrial cancer based on multiple machine learning models

JOURNAL=Frontiers in Genetics

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2025.1687922

DOI=10.3389/fgene.2025.1687922

ISSN=1664-8021

ABSTRACT=BackgroundSenescence-related lncRNAs (srlncRNA) mediate carcinogenesis in various malignancies. However, its roles in endometrial cancer (EC) remain unknown. Our research aims to construct a predictive srlncRNA model with prognostic and therapeutic significance in EC.MethodsWe first downloaded the gene expression and medical information from the TCGA, as well as senescence-related lncRNAs (srlncRNAs) from the CellAge databases. Then, a co-expression network of cell senescence-related mRNA−lncRNA was explored with R. Subsequently, we performed Cox and Lasso regression and machine learning analysis to identify srlncRNAs related to the prognosis of EC and built a predictive model. Continually, we drew a nomogram to improve its ability to predict prognosis. Further, GSEA was used to explore potential mechanisms. Differences in TME, immune infiltrating cells, and checkpoints of the two risk groups were compared using GSEA and CIBERSORT. Finally, the drug sensitivity of patient-derived tumor organoids (PDOs) was investigated.ResultsWe first built a prognostic model based on seven srlncRNAs (AL121906.2, AP002761.4, BX322234.1, LINC00662, LINC00908, VIM-AS1, and ZNF236-DT). The model, which was screened by machine learning, functioned well in three sets with good stability and accuracy. Furthermore, the nomogram based on age, grade, and risk scores could precisely predict the prognosis of EC patients. The AUC of risk scores was highest compared to other clinical parameters (AUC risk score = 0.769, AUC age = 0.615, and AUC grade = 0.681). This srlncRNAs were enriched in the cell cycle, certain malignant tumors, and cancer-associated regulatory pathways. Afterward, low-risk EC patients had more immune-infiltrating cells and may benefit from anti-PD-1 and anti-CTLA4 treatment. Paclitaxel, gemcitabine, and cisplatin (all p < 0.05) may be more useful in EC patients with high expression of targeted srlncRNAs in the GDSC database. The levels of targeted srlncRNAs and drug sensitivity varied significantly among different EC PDOs. The EC-18 PDO was more resistant to three drugs, which aligned with clinical observation.ConclusionThe srlncRNA signature (AL121906.2, AP002761.4, BX322234.1, LINC00662, LINC00908, VIM-AS1, and ZNF236-DT) could guide prognosis prediction and treatment choices for EC patients.