AUTHOR=Miao Hui , Zhu Yunke , Zheng Jiaqi , Deng Chunfeng , Zeng Yi , Tang Fei , Liu Xi 

TITLE=Identification and experimental validation of demethylation-related genes in diabetic nephropathy

JOURNAL=Frontiers in Genetics

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2025.1675592

DOI=10.3389/fgene.2025.1675592

ISSN=1664-8021

ABSTRACT=BackgroundDiabetic nephropathy (DN) is a major microvascular complication of diabetes, and its pathogenesis is closely associated with abnormal epigenetic regulation, particularly the silencing of tumor suppressor genes due to hypermethylation of promoter regions. This study was to investigate the workings of demethylation in diabetic nephropathy by applying bioinformatics methods.MethodsDN-related datasets (GSE142153 and GSE154881) and demethylation-related genes (D-RGs) were included. Differentially expressed genes (DEGs) (DN vs. normal) were obtained. Candidate genes were obtained from the intersection of DEGs and D-RGs. To identify key genes, the Least absolute shrinkage and selection operator (LASSO) and Boruta algorithm, and expression validation were used for screening. The expression validation was used to identify biomarkers. The receiver operating characteristic (ROC) curve was subsequently utilized to assess the biomarkers’ capability to distinguish diseased from normal samples. Subsequently, a predictive nomogram was created to estimate the likelihood of developing DN. In addition, functional enrichment, immune infiltration, subcellular localization, correlation of biomarker expression with renal function, correlation for other diseases, network analysis of molecular interactions and computational drug prediction were carried out. Lastly, Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR) was carried out to confirm the expression levels of biomarkers in blood samples.ResultsCXCL2 and MLF1 were determined to be biomarkers that exhibited notably elevated expression levels in the DN, in contrast to the normal group. Then the nomogram network was built, which had high prediction accuracy. Pathways most significantly enriched by CXCL2 and MLF1 included cytokine-cytokine receptor interaction and MAPK signaling pathway. Five types of immune cells were identified by immune infiltration analysis. In the RNA binding protein (RBP) -mRNA regulatory network, seven pathways were co-enriched in both biomarkers. In the TF-mRNA regulatory network, TFs shared by both biomarkers include JUN, GATA2, and SRF. Finally drug prediction analysis found a total of 172 target drugs for CXCL2 and MLF1. RT-qPCR experiment revealed that both biomarkers showed a notable rise in the DN group relative to the normal group. RT-qPCR results revealed the DN exhibited notably increased expression levels of the two biomarkers (CXCL2 and MLF1) compared to the normal group.ConclusionCXCL2 and MLF1 were identified as diagnostic biomarkers for DN, offering a new reference for its treatment.