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<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Food Sci. Technol.</journal-id>
<journal-title-group>
<journal-title>Frontiers in Food Science and Technology</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Food Sci. Technol.</abbrev-journal-title>
</journal-title-group>
<issn pub-type="epub">2674-1121</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="publisher-id">1667337</article-id>
<article-id pub-id-type="doi">10.3389/frfst.2025.1667337</article-id>
<article-version article-version-type="Version of Record" vocab="NISO-RP-8-2008"/>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Original Research</subject>
</subj-group>
</article-categories>
<title-group>
<article-title>Status of maize seed systems and storage practices among smallholder farmers in Tanzania</article-title>
<alt-title alt-title-type="left-running-head">Mlay et al.</alt-title>
<alt-title alt-title-type="right-running-head">
<ext-link ext-link-type="uri" xlink:href="https://doi.org/10.3389/frfst.2025.1667337">10.3389/frfst.2025.1667337</ext-link>
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<contrib-group>
<contrib contrib-type="author" corresp="yes">
<name>
<surname>Mlay</surname>
<given-names>Mary Raymond</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<xref ref-type="corresp" rid="c001">&#x2a;</xref>
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<contrib contrib-type="author">
<name>
<surname>Yustas</surname>
<given-names>Yusto Mugisha</given-names>
</name>
<xref ref-type="aff" rid="aff3">
<sup>3</sup>
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<contrib contrib-type="author">
<name>
<surname>Majubwa</surname>
<given-names>Ramadhani Omari</given-names>
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<sup>1</sup>
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<aff id="aff1">
<label>1</label>
<institution>Department of Crop Science and Horticulture, Sokoine University of Agriculture, Chuo Kikuu</institution>, <city>Morogoro</city>, <country country="TZ">Tanzania</country>
</aff>
<aff id="aff2">
<label>2</label>
<institution>Ministry of Agriculture Training Institute (MATI) - Uyole</institution>, <city>Mbeya</city>, <country country="TZ">Tanzania</country>
</aff>
<aff id="aff3">
<label>3</label>
<institution>Department of Food Science and Agro Processing, School of Engineering and Technology, Sokoine University of Agriculture, Chuo Kikuu</institution>, <city>Morogoro</city>, <country country="TZ">Tanzania</country>
</aff>
<author-notes>
<corresp id="c001">
<label>&#x2a;</label>Correspondence: Mary Raymond Mlay, <email xlink:href="mailto:mkunderaymond390@gmail.com">mkunderaymond390@gmail.com</email>; Yusto Mugisha Yustas, <email>engyustasyusto@sua.ac.tz</email>
</corresp>
</author-notes>
<pub-date publication-format="electronic" date-type="pub" iso-8601-date="2026-02-12">
<day>12</day>
<month>02</month>
<year>2026</year>
</pub-date>
<pub-date publication-format="electronic" date-type="collection">
<year>2025</year>
</pub-date>
<volume>5</volume>
<elocation-id>1667337</elocation-id>
<history>
<date date-type="received">
<day>28</day>
<month>08</month>
<year>2025</year>
</date>
<date date-type="rev-recd">
<day>15</day>
<month>12</month>
<year>2025</year>
</date>
<date date-type="accepted">
<day>18</day>
<month>12</month>
<year>2025</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#xa9; 2026 Mlay, Yustas and Majubwa.</copyright-statement>
<copyright-year>2026</copyright-year>
<copyright-holder>Mlay, Yustas and Majubwa</copyright-holder>
<license>
<ali:license_ref start_date="2026-02-12">https://creativecommons.org/licenses/by/4.0/</ali:license_ref>
<license-p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License (CC BY)</ext-link>. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</license-p>
</license>
</permissions>
<abstract>
<p>The quality and productivity of maize crops are functions of the planting materials and crop management practices. However, the quality and storage practices of farm-saved maize seed, used by more than 70% of smallholder farmers in Tanzania, are less well studied. This study aimed to assess the status of maize seed systems, storage practices, and the quality of stored seeds in the Kilosa, Gairo, and Mvomero districts of the Morogoro region. A multi-stage stratified sampling approach was applied to survey 240 maize farmers across 11 villages in the three districts. Data were collected through semi-structured questionnaires and focus group discussions. Survey data were analysed using R software using a chi-square test (<italic>p</italic> &#x2264; 0.05) to examine relationships between variables. In addition, a second study was conducted using a 3 &#xd7; 3 factorial experiment arranged in a completely randomised design (CRD). Maize seed samples from the three most common storage materials were collected at harvest and after 3&#xa0;months of storage for laboratory analysis of seed quality parameters: germination percentage, vigour index, and moisture content. The data were subjected to ANOVA followed by Tukey&#x2019;s HSD test (<italic>p</italic> &#x2264; 0.05) in R software. Results revealed that 56.2% of farmers used informal seed systems, and 70.8% engaged in seed recycling due to financial constraints. Education level significantly influenced the choice of maize seed system (<italic>p</italic> &#x3d; 0.004), maize cultivar (<italic>p</italic> &#x3d; 0.025), and maize seed recycling practice (<italic>p</italic> &#x3d; 0.023). Polypropylene bags (with and without insecticide) and hermetic bags were the most common storage materials. The hermetic bag maintained a higher seed germination rate (86.8%) than the polypropylene bag without insecticide (81.8%). Hermetic bags are therefore recommended over polypropylene bags (with and without insecticide) for enhancing maize seed storage. Capacity building on seed systems should prioritise farmer education as it significantly determines the seed systems and handling practices adopted.</p>
</abstract>
<kwd-group>
<kwd>farmers saved seeds</kwd>
<kwd>hermetic storage</kwd>
<kwd>porous bag</kwd>
<kwd>seed damage</kwd>
<kwd>seed systems</kwd>
</kwd-group>
<funding-group>
<funding-statement>The author(s) declared that financial support was not received for this work and/or its publication.</funding-statement>
</funding-group>
<counts>
<fig-count count="2"/>
<table-count count="8"/>
<equation-count count="4"/>
<ref-count count="38"/>
<page-count count="15"/>
</counts>
<custom-meta-group>
<custom-meta>
<meta-name>section-at-acceptance</meta-name>
<meta-value>Food Packaging and Preservation</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
<body>
<sec sec-type="intro" id="s1">
<label>1</label>
<title>Introduction</title>
<p>Maize (<italic>Zea mays</italic> L.) is an adaptable and multi-purpose crop (<xref ref-type="bibr" rid="B11">Erenstein et al., 2022</xref>), commonly used for human food, animal feed, and as a raw material in various industries (<xref ref-type="bibr" rid="B26">Serna-Saldivar, 2023</xref>). It is a staple food for more than 300 million individuals in sub-Saharan Africa (<xref ref-type="bibr" rid="B5">Badu-Apraku et al., 2017</xref>) and serves as a major food and cash crop in most parts of Tanzania (<xref ref-type="bibr" rid="B19">Mtaki, 2019</xref>). Tanzania ranks first in maize production in East Africa and fourth across Africa (<xref ref-type="bibr" rid="B22">Ngalya, 2019</xref>). Maize accounts for approximately 50% of rural cash income and constitutes more than 80% of all cereals planted in the country (<xref ref-type="bibr" rid="B21">Mutanyagwa et al., 2018</xref>). Its production is dominated by small-scale farmers, who contribute over 80% of Tanzania&#x2019;s maize output (<xref ref-type="bibr" rid="B19">Mtaki, 2019</xref>). Maize is grown in all agro-ecological zones of Tanzania, mainly under rain-fed conditions, and is characterised by low-input farming, minimal mechanisation, limited use of agrochemicals, and a high rate of seed saving (<xref ref-type="bibr" rid="B6">Baijukya et al., 2020</xref>).</p>
<p>In the Morogoro Region, a key part of Tanzania and one of the major maize-growing areas in the country&#x2019;s eastern zone, maize accounts for approximately 47% of the total area planted with annual crops. Farmers save maize seed by selecting healthy, vigorous, pest-tolerant, and disease-tolerant plants from their harvest and saving and reusing them as seed in the following season. Although this practice helps smallholder farmers to reduce production costs, it can compromise seed quality if proper storage and handling practices are not observed.</p>
<p>Seed is the single most significant input in crop production (<xref ref-type="bibr" rid="B4">Aqil, 2020</xref>) as it carries a variety&#x2019;s genetic potential and determines the productivity of all other inputs. Effective use of other inputs maximises the genetic potential of the seed variety, making quality seed a fundamental prerequisite for an economically viable food production system. Seed quality is defined by parameters such as varietal purity, specific purity, germinability, vigour, health status, and moisture content. Less than 20% of cultivated land in Africa is planted with improved seed, mainly due to limited accessibility and affordability (<xref ref-type="bibr" rid="B12">FARMAFRICA, 2022</xref>). According to Biemont (2013), formal and informal seed systems enable farmers to access seeds in their localities. The formal seed system is a deliberately constructed framework comprising a series of activities that produce genetically improved, certified seed varieties. In contrast, the informal seed system involves farmers producing traditional or improved varieties without oversight from regulatory bodies that ensure quality control (<xref ref-type="bibr" rid="B12">FARMAFRICA, 2022</xref>).</p>
<p>Maintaining seed quality during storage is a significant challenge for smallholder farmers. For instance, postharvest losses of dry cereals are estimated at 10%&#x2013;20% (<xref ref-type="bibr" rid="B28">Sugri et al., 2021</xref>). These losses reduce the quantity of viable seed available for the next planting season and are influenced by factors such as pest infestation, high humidity, and elevated temperatures (<xref ref-type="bibr" rid="B4">Aqil, 2020</xref>). Therefore, proper postharvest handling practices are crucial for maintaining seed quality and prolonging storage life. High-quality seed can be ensured through appropriate harvesting and postharvest techniques, including selecting healthy cobs, shelling, drying, seed sorting, proper packaging, and storage (<xref ref-type="bibr" rid="B35">Yousaf et al., 2016</xref>). Understanding the dynamics of seed sourcing and handling, along with the associated challenges in Tanzania, is essential to maintaining seed viability, improving crop yields, and enhancing food security.</p>
<p>Despite maize&#x2019;s importance and the widespread reliance on farm-saved seed among smallholders, there is limited research on the actual seed systems, storage practices, and seed quality among these farmers in Tanzania. Most previous studies have focused on improved or certified seeds, neglecting the informal systems that feed most of the population. This represents a critical research gap as understanding smallholders&#x2019; practices and challenges is essential to improving seed quality, reducing postharvest losses, and supporting sustainable maize production.</p>
<p>The study recognises that maize seed quality among smallholder farmers is influenced by a combination of factors, including seed sources, farmer socio-economic and demographic characteristics, and storage practices. These interconnected factors collectively determine the viability, germination potential, and overall quality of stored maize seed, providing a conceptual context for investigating seed management practices in the study area.</p>
<p>This study, therefore, aims to assess the current maize seed sources and storage practices used by smallholder farmers in the Morogoro Region. The findings will provide insights into existing practices and offer recommendations to enhance seed preservation and sustainable agricultural productivity. Furthermore, the study will inform key stakeholders on strategies to improve farmer productivity through maize seed systems, seed sources, and seed-handling practices.</p>
</sec>
<sec sec-type="materials|methods" id="s2">
<label>2</label>
<title>Materials and methods</title>
<sec id="s2-1">
<label>2.1</label>
<title>Description of the study area</title>
<p>The survey was conducted from July to November 2023 in 11 villages across the Kilosa, Gairo, and Mvomero districts in the Morogoro Region, eastern Tanzania (<xref ref-type="fig" rid="F1">Figure 1</xref>). The study area lies at elevations ranging from approximately 300 to 2,200&#xa0;m above sea level and experiences a warm tropical climate, with mean temperatures ranging from 18.5 &#xb0;C to 29.8 &#xb0;C and moderate relative humidity. Rainfall is bimodal, with short rains occurring from October to December and long rains from March to May, with an annual average of 600&#x2013;1,200&#xa0;mm.</p>
<fig id="F1" position="float">
<label>FIGURE 1</label>
<caption>
<p>Map of the Morogoro region indicating the study locations.</p>
</caption>
<graphic xlink:href="frfst-05-1667337-g001.tif">
<alt-text content-type="machine-generated">Map showing the Morogoro region of Tanzania with Gairo, Kilosa, and Mvomero districts highlighted in different colors. Various wards and selected study site villages are labeled, with a scale bar and compass for orientation.</alt-text>
</graphic>
</fig>
<p>The study area is predominantly rural, with agriculture as the main livelihood. Soils are mainly sandy loam and clay loam, which are suitable for maize production. The selected villages were purposively chosen based on their high maize production and representation of typical smallholder farming systems in Tanzania&#x2019;s eastern maize-growing zone.</p>
</sec>
<sec id="s2-2">
<label>2.2</label>
<title>Sample size and sampling</title>
<p>The sample size of respondents was determined according to <xref ref-type="bibr" rid="B31">Uakarn et al. (2021)</xref> using the Cochran formula (<xref ref-type="disp-formula" rid="e1">Equation 1</xref>). The formula assumes that the population size is large and unknown.<disp-formula id="e1">
<mml:math id="m1">
<mml:mrow>
<mml:mi mathvariant="normal">N</mml:mi>
<mml:mo>&#x3d;</mml:mo>
<mml:mfrac>
<mml:mrow>
<mml:mtext>&#x2002;</mml:mtext>
<mml:mi mathvariant="normal">P</mml:mi>
<mml:mrow>
<mml:mfenced open="(" close=")" separators="|">
<mml:mrow>
<mml:mn>1</mml:mn>
<mml:mo>&#x2212;</mml:mo>
<mml:mi mathvariant="normal">p</mml:mi>
</mml:mrow>
</mml:mfenced>
</mml:mrow>
<mml:mi mathvariant="normal">z</mml:mi>
<mml:mn>2</mml:mn>
</mml:mrow>
<mml:mrow>
<mml:mi>e</mml:mi>
<mml:mn>2</mml:mn>
</mml:mrow>
</mml:mfrac>
<mml:mo>,</mml:mo>
</mml:mrow>
</mml:math>
<label>(1)</label>
</disp-formula>
<disp-formula id="equ1">
<mml:math id="m2">
<mml:mrow>
<mml:mi mathvariant="normal">N</mml:mi>
<mml:mo>&#x3d;</mml:mo>
<mml:mfrac>
<mml:mrow>
<mml:mtext>&#x2002;</mml:mtext>
<mml:mn>0.1</mml:mn>
<mml:mrow>
<mml:mfenced open="(" close=")" separators="|">
<mml:mrow>
<mml:mn>1</mml:mn>
<mml:mo>&#x2212;</mml:mo>
<mml:mn>0.1</mml:mn>
</mml:mrow>
</mml:mfenced>
</mml:mrow>
<mml:mrow>
<mml:mfenced open="(" close=")" separators="|">
<mml:mrow>
<mml:mn>2.58</mml:mn>
</mml:mrow>
</mml:mfenced>
</mml:mrow>
<mml:mn>2</mml:mn>
<mml:mo>&#x3d;</mml:mo>
<mml:mn>240</mml:mn>
</mml:mrow>
<mml:mn>0.052</mml:mn>
</mml:mfrac>
<mml:mo>,</mml:mo>
</mml:mrow>
</mml:math>
</disp-formula>
</p>
<p>where N represents the required sample size, p represents the estimated proportion of the population possessing the attribute of interest (<italic>p</italic> &#x3d; 0.1), z represents the value at reliability level or significance level (<italic>z</italic> &#x3d; 2.58), and e represents the acceptable sampling error (<italic>e</italic> &#x3d; 0.05). Based on the calculations, a total of 240 respondents (<xref ref-type="table" rid="T1">Table 1</xref>) were selected and distributed among study villages in the three districts based on the farmers&#x2019; population size: Dumila (33), Msowero (36), and Magubike (33) in the Kilosa district, Kibati (32), and Sungaji (35) in the Mvomero district, and Rubeho (34), and Kibedya (37) in the Gairo district.</p>
<table-wrap id="T1" position="float">
<label>TABLE 1</label>
<caption>
<p>Distribution of respondents per district.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th align="left">District</th>
<th align="left">Ward</th>
<th align="left">Village</th>
<th align="left">Respondent frequency</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td align="left">Kilosa</td>
<td align="left">Dumila</td>
<td align="left">Kwambe</td>
<td align="left">14</td>
</tr>
<tr>
<td align="left">Kilosa</td>
<td align="left">Dumila</td>
<td align="left">Dumila</td>
<td align="left">19</td>
</tr>
<tr>
<td align="left">Kilosa</td>
<td align="left">Msowero</td>
<td align="left">Majamba</td>
<td align="left">36</td>
</tr>
<tr>
<td align="left">Kilosa</td>
<td align="left">Magubike</td>
<td align="left">Magubike</td>
<td align="left">33</td>
</tr>
<tr>
<td align="left">Mvomero</td>
<td align="left">Kibati</td>
<td align="left">Hoza</td>
<td align="left">12</td>
</tr>
<tr>
<td align="left">Mvomero</td>
<td align="left">Kibati</td>
<td align="left">Sarawe</td>
<td align="left">10</td>
</tr>
<tr>
<td align="left">Mvomero</td>
<td align="left">Kibati</td>
<td align="left">Diburuma</td>
<td align="left">10</td>
</tr>
<tr>
<td align="left">Mvomero</td>
<td align="left">Sungaji</td>
<td align="left">Kigugu</td>
<td align="left">17</td>
</tr>
<tr>
<td align="left">Mvomero</td>
<td align="left">Sungaji</td>
<td align="left">Mbogo</td>
<td align="left">18</td>
</tr>
<tr>
<td align="left">Gairo</td>
<td align="left">Rubeho</td>
<td align="left">Kwipipa</td>
<td align="left">34</td>
</tr>
<tr>
<td align="left">Gairo</td>
<td align="left">Kibedya</td>
<td align="left">Ihenje</td>
<td align="left">37</td>
</tr>
<tr>
<td align="left">Total</td>
<td align="left">&#x200b;</td>
<td align="left">&#x200b;</td>
<td align="left">240</td>
</tr>
</tbody>
</table>
</table-wrap>
</sec>
<sec id="s2-3">
<label>2.3</label>
<title>Data collection of maize seed systems and storage practices</title>
<p>A multi-stage stratified sampling technique was used to select respondents (<xref ref-type="bibr" rid="B33">Wanyama et al., 2023</xref>). Kilosa, Gairo, and Mvomero districts were purposively selected based on their popularity in maize production. At least two wards were selected per district, and from each, three (3) villages were randomly selected. A random sample of at least 30 maize farmers was drawn from each village with assistance from village extension officers (<xref ref-type="table" rid="T1">Table 1</xref>). The sampling technique was considered cost-effective and ensured equal representation of the target population (<xref ref-type="bibr" rid="B39">Samuel and Mekebu, 2025</xref>).</p>
<p>During the survey, data on the farm-saved maize seed system and storage practices were collected, including demographic characteristics, maize seed systems, cultivars used, seed recycling practices, storage and treatment practices, and causes of postharvest seed losses. Data were collected using semi-structured questionnaires (Appendix 1) and focus group discussions (FGDs). For the FGDs, participants were purposively selected to include experienced maize farmers, both male and female, who actively practice seed recycling and storage. Each FGD consisted of 8&#x2013;12 participants per village to facilitate in-depth discussions. Guiding questions addressed farmers&#x2019; seed selection criteria, storage techniques, challenges in maintaining seed quality, experiences with different storage materials, and perceived causes of postharvest losses. Discussions were moderated by trained facilitators and recorded for subsequent thematic analysis.</p>
</sec>
<sec id="s2-4">
<label>2.4</label>
<title>Ethical considerations and data transparency</title>
<p>The study followed strict ethical guidelines to protect the rights and privacy of all participants. Written informed consent for participation was not required from participants or their legal guardians/next of kin as verbal (not written) informed consent was obtained from participants due to limited literacy in the study area.</p>
<p>All research procedures were conducted in compliance with the ethical review requirements of Sokoine University of Agriculture, and approval was obtained from the institutional research ethics committee. To ensure anonymity and confidentiality, all questionnaire and focus group discussion data were coded, stored securely, and were only accessible to the research team. Personal identifiers were removed during data analysis and reporting, ensuring that no individual participants could be identified.</p>
</sec>
<sec id="s2-5">
<label>2.5</label>
<title>Laboratory assessment of seed quality</title>
<p>In addition to survey data collection, random samples of 1&#xa0;kg of farmers&#x2019; stored maize seed were collected from the three most common storage materials in the surveyed area. Laboratory analysis procedures were as follows.</p>
<sec id="s2-5-1">
<label>2.5.1</label>
<title>Data collection on the quality of farmer-saved maize seed for laboratory analysis</title>
<p>A 3 &#xd7; 3 factorial experiment was conducted using a completely randomised design (CRD). The two experimental factors were as follows: (i) seed storage material with three levels (polypropylene bag without insecticide, hermetic bag (PICS), and polypropylene bag treated with insecticide) and (ii) storage location with three levels (Kilosa, Gairo, and Mvomero districts). This factorial arrangement yielded nine treatment combinations; each replicated three times. The widely grown maize variety <italic>Stuka</italic> was selected to maintain varietal uniformity and minimise genetic variability among samples.</p>
<p>Farmers&#x2019; saved maize seed was sampled from three farmers in each district at harvest (August 2023) and after 3&#xa0;months of storage (November 2023). Each farmer stored maize seed using all three storage materials under typical farmer-managed conditions. Seeds were stored inside farmers&#x2019; houses, on raised platforms, in well-ventilated areas to simulate common local storage practices. Environmental conditions, including temperature and relative humidity, were not actively controlled or monitored during the storage period, reflecting typical farmer-managed storage conditions. Participating farmers were purposely selected based on their use of the same maize variety (Stuka), harvesting in the same production season, and applying comparable field management and postharvest handling practices. This selection minimised variability arising from pre-harvest factors and ensured that differences in seed quality were primarily attributable to storage conditions rather than field-level differences.</p>
<p>From each storage replicate, a random 1&#xa0;kg sample was collected for laboratory determination of germination percentage, seedling vigour index, and moisture content. In the laboratory, samples were randomly assigned to tests to ensure equal representation within the experimental layout and to minimise systematic errors. Analyses were performed according to standardised protocols to ensure consistent handling of all samples. Although formal blinding of sample identity was not applied, adherence to uniform procedures minimised potential observer bias. It ensured reliable, objective measurement of the evaluated parameters (germination percentage, vigour index, and moisture content).</p>
<sec id="s2-5-1-1">
<label>2.5.1.1</label>
<title>Germination test</title>
<p>Germination tests were conducted according to the International Seed Testing Association (<xref ref-type="bibr" rid="B38">ISTA, 2022</xref>) guidelines, using sieved sand as a planting medium. Sand was sieved through a 2&#xa0;mm wire mesh to remove larger particles. In each plastic dish, maize seeds were placed on an even layer of moist sand and covered with a 10&#xa0;mm layer of loose sand. Seeds were watered every 2&#xa0;days. Each dish contained 50 seeds per replicate, with a total of 200 seeds per treatment. The dishes were incubated at room temperature (27 &#xb0;C) for 7&#xa0;days, after which final seedling evaluations were conducted. Data collected included the numbers of normal, abnormal, and ungerminated seedlings, which were used to calculate germination percentage, moisture content, and vigour index using <xref ref-type="disp-formula" rid="e2">Equation 2</xref>.<disp-formula id="e2">
<mml:math id="m3">
<mml:mrow>
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<mml:mtext>normal</mml:mtext>
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<mml:mtext>seedlings</mml:mtext>
</mml:mrow>
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<mml:mtext>&#x2009;</mml:mtext>
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<mml:mtext>&#x2009;</mml:mtext>
<mml:mi>s</mml:mi>
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<mml:mi>w</mml:mi>
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<mml:mo>&#xd7;</mml:mo>
<mml:mn>100</mml:mn>
</mml:mrow>
</mml:mfrac>
<mml:mo>.</mml:mo>
</mml:mrow>
</mml:math>
<label>(2)</label>
</disp-formula>
</p>
</sec>
<sec id="s2-5-1-2">
<label>2.5.1.2</label>
<title>Seedling vigour index</title>
<p>Seedlings from the standard germination tests were used to evaluate vigour according to the method of <xref ref-type="bibr" rid="B8">Bhadauria et al. (2019)</xref>. Seedling samples from each dish were measured for shoot and root lengths (cm). Mean root and shoot length were used to calculate the seedling vigour index (S.V.I) following the formula described by <xref ref-type="bibr" rid="B1">Abdul-Baki and Anderson (1973)</xref> is shown in <xref ref-type="disp-formula" rid="e3">Equation 3</xref>.<disp-formula id="e3">
<mml:math id="m4">
<mml:mrow>
<mml:mi mathvariant="normal">S</mml:mi>
<mml:mo>.</mml:mo>
<mml:mtext>&#x2009;</mml:mtext>
<mml:mi mathvariant="normal">V</mml:mi>
<mml:mo>.</mml:mo>
<mml:mtext>&#x2009;</mml:mtext>
<mml:mi mathvariant="normal">I</mml:mi>
<mml:mo>.</mml:mo>
<mml:mo>&#x3d;</mml:mo>
<mml:mtext>Germination&#x2009;</mml:mtext>
<mml:mrow>
<mml:mfenced open="(" close=")" separators="|">
<mml:mrow>
<mml:mo>%</mml:mo>
</mml:mrow>
</mml:mfenced>
</mml:mrow>
<mml:mo>&#xd7;</mml:mo>
<mml:mtext>Mean&#x2009;seedling&#x2009;length&#x2009;</mml:mtext>
<mml:mrow>
<mml:mfenced open="(" close=")" separators="|">
<mml:mrow>
<mml:mtext>cm</mml:mtext>
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</mml:mrow>
</mml:math>
<label>(3)</label>
</disp-formula>
</p>
</sec>
<sec id="s2-5-1-3">
<label>2.5.1.3</label>
<title>Seed moisture content</title>
<p>Seed moisture content (MC) was measured following <xref ref-type="bibr" rid="B25">Rahmawati et al. (2019)</xref> at harvest and after 3&#xa0;months of farmers&#x2019; storage in the study villages. Three samples per treatment were tested for MC using a portable direct-reading moisture meter (Dole Grain Moisture Tester, model 500).</p>
</sec>
</sec>
</sec>
<sec id="s2-6">
<label>2.6</label>
<title>Data analysis</title>
<p>Questionnaire data were first coded and cleaned using IBM SPSS software and then imported into R software (Version 4.3.3) for statistical analysis. Descriptive statistics, including means, frequencies, and percentages, were computed to summarise the information obtained from the questionnaires. Contingency chi-square tests (&#x3c7;<sup>2</sup>) were conducted to examine relationships between categorical variables, with significance determined at <italic>p</italic> &#x2264; 0.05. Complete test statistics, including &#x3c7;<sup>2</sup> values and <italic>p</italic>-values, were reported. Data from the focus group discussion were analysed using thematic and content analysis.</p>
<p>Laboratory data on seed quality were subjected to analysis of variance (ANOVA) using R software (version 4.3.3). Prior to analysis, all assumptions of normality were checked and found to be satisfied. Tukey&#x2019;s HSD test was applied for multiple mean comparisons at <italic>p</italic> &#x2264; 0.05 to identify significant differences among treatment combinations. All data were complete, with no missing values or outliers detected.</p>
</sec>
</sec>
<sec sec-type="results" id="s3">
<label>3</label>
<title>Results</title>
<sec id="s3-1">
<label>3.1</label>
<title>Demographic characteristics</title>
<p>Many maize farmers involved in this study were male (58.8%). Most respondents had completed primary school (77.5%). Most farmers were adults aged 26&#x2013;59&#xa0;years (82.8%) (<xref ref-type="table" rid="T2">Table 2</xref>).</p>
<table-wrap id="T2" position="float">
<label>TABLE 2</label>
<caption>
<p>Demographic distribution of maize farmers in the surveyed area.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th rowspan="2" align="left">District</th>
<th colspan="2" align="left">Sex (%)</th>
<th colspan="3" align="left">Age (%)</th>
<th colspan="4" align="left">Education (%)</th>
</tr>
<tr>
<th align="left">F</th>
<th align="left">M</th>
<th align="left">Young (19&#x2013;25)</th>
<th align="left">Adult (26&#x2013;59)</th>
<th align="left">Elder (&#x2265;60)</th>
<th align="left">No education</th>
<th align="left">Primary</th>
<th align="left">Secondary</th>
<th align="left">Tertiary</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td colspan="10" align="center">Farmers&#x2019; percentage (%) based on demographic characteristics (n &#x3d; 240)</td>
</tr>
<tr>
<td align="left">Kilosa</td>
<td align="left">15.0</td>
<td align="left">27.5</td>
<td align="left">0.00</td>
<td align="left">36.2</td>
<td align="left">6.25</td>
<td align="left">5.83</td>
<td align="left">31.2</td>
<td align="left">4.17</td>
<td align="left">1.25</td>
</tr>
<tr>
<td align="left">Gairo</td>
<td align="left">15.8</td>
<td align="left">13.8</td>
<td align="left">2.08</td>
<td align="left">25.4</td>
<td align="left">2.08</td>
<td align="left">2.08</td>
<td align="left">24.6</td>
<td align="left">2.08</td>
<td align="left">0.83</td>
</tr>
<tr>
<td align="left">Mvomero</td>
<td align="left">10.4</td>
<td align="left">17.5</td>
<td align="left">0.42</td>
<td align="left">21.2</td>
<td align="left">6.25</td>
<td align="left">2.50</td>
<td align="left">21.7</td>
<td align="left">2.08</td>
<td align="left">1.67</td>
</tr>
<tr>
<td align="left">Total (%)</td>
<td align="left">41.2</td>
<td align="left">58.8</td>
<td align="left">2.5</td>
<td align="left">82.8</td>
<td align="left">14.58</td>
<td align="left">10.41</td>
<td align="left">77.5</td>
<td align="left">8.33</td>
<td align="left">3.75</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn>
<p>Source: Field survey.</p>
</fn>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="s3-2">
<label>3.2</label>
<title>Status of the maize seed system</title>
<sec id="s3-2-1">
<label>3.2.1</label>
<title>Maize seed system used</title>
<p>Generally, many maize farmers (56.2%) rely on informal seed systems. The level of education of respondents significantly (<italic>p</italic> &#x3d; 0.004498) influenced farmers&#x2019; decision regarding which maize seed system to use (<xref ref-type="table" rid="T3">Table 3</xref>). Many respondents with a primary education level (42.1%) opted for the informal maize seed system, compared with 0.8% of respondents with a tertiary education.</p>
<table-wrap id="T3" position="float">
<label>TABLE 3</label>
<caption>
<p>Maize seed system, cultivar used, and recycling practice in the surveyed area in relation to farmers&#x2019; demographic characteristics.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th rowspan="2" align="left">Variable</th>
<th rowspan="2" align="left">Category</th>
<th colspan="2" align="left">Sex (%)<break/>(n &#x3d; 240)</th>
<th colspan="3" align="left">Age (%) (n &#x3d; 240)</th>
<th colspan="4" align="left">Education (%) (n &#x3d; 240)</th>
<th align="left">Total (%)</th>
</tr>
<tr>
<th align="left">F</th>
<th align="left">M</th>
<th align="left">Young (19&#x2013;25)</th>
<th align="left">Adult (26&#x2013;59)</th>
<th align="left">Elder (&#x2265;60)</th>
<th align="left">No formal education</th>
<th align="left">Primary</th>
<th align="left">Secondary</th>
<th align="left">Tertiary</th>
<th align="left">Total (%)</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td colspan="12" align="center">Farmers&#x2019; count and significance based on demographic characteristics</td>
</tr>
<tr>
<td rowspan="3" align="left">Maize seed system</td>
<td align="left">Formal</td>
<td align="left">18.8</td>
<td align="left">25.0</td>
<td align="left">0.8</td>
<td align="left">37.5</td>
<td align="left">5.4</td>
<td align="left">1.7</td>
<td align="left">35.4</td>
<td align="left">3.8</td>
<td align="left">2.9</td>
<td align="left">43.8</td>
</tr>
<tr>
<td align="left">Informal</td>
<td align="left">22.5</td>
<td align="left">33.8</td>
<td align="left">1.7</td>
<td align="left">45.4</td>
<td align="left">9.2</td>
<td align="left">8.8</td>
<td align="left">42.1</td>
<td align="left">4.6</td>
<td align="left">0.8</td>
<td align="left">56.2</td>
</tr>
<tr>
<td align="left">&#x3c7;<sup>2</sup> (<italic>p</italic>-value)</td>
<td align="left">&#x200b;</td>
<td align="left">
<bold>0.754</bold>
</td>
<td align="left">&#x200b;</td>
<td align="left">&#x200b;</td>
<td align="left">
<bold>0.660</bold>
</td>
<td align="left">&#x200b;</td>
<td align="left">&#x200b;</td>
<td align="left">&#x200b;</td>
<td align="left">
<bold>0.004</bold>
</td>
<td align="left">&#x200b;</td>
</tr>
<tr>
<td rowspan="4" align="left">Maize cultivar used</td>
<td align="left">Hybrid</td>
<td align="left">16.3</td>
<td align="left">21.3</td>
<td align="left">0.8</td>
<td align="left">32.9</td>
<td align="left">3.8</td>
<td align="left">1.3</td>
<td align="left">31.3</td>
<td align="left">2.5</td>
<td align="left">2.5</td>
<td align="left">37.5</td>
</tr>
<tr>
<td align="left">Landrace</td>
<td align="left">18.3</td>
<td align="left">22.9</td>
<td align="left">1.7</td>
<td align="left">32.5</td>
<td align="left">7.1</td>
<td align="left">7.1</td>
<td align="left">30.0</td>
<td align="left">3.8</td>
<td align="left">0.4</td>
<td align="left">41.2</td>
</tr>
<tr>
<td align="left">OPV</td>
<td align="left">6.7</td>
<td align="left">14.6</td>
<td align="left">0.0</td>
<td align="left">17.5</td>
<td align="left">3.8</td>
<td align="left">2.1</td>
<td align="left">16.3</td>
<td align="left">2.1</td>
<td align="left">0.8</td>
<td align="left">21.2</td>
</tr>
<tr>
<td align="left">&#x3c7;<sup>2</sup> (<italic>p</italic>-value)</td>
<td align="left">&#x200b;</td>
<td align="left">
<bold>0.268</bold>
</td>
<td align="left">&#x200b;</td>
<td align="left">&#x200b;</td>
<td align="left">
<bold>0.323</bold>
</td>
<td align="left">&#x200b;</td>
<td align="left">&#x200b;</td>
<td align="left">&#x200b;</td>
<td align="left">
<bold>0.025</bold>
</td>
<td align="left">&#x200b;</td>
</tr>
<tr>
<td rowspan="3" align="left">Maize seed recycling</td>
<td align="left">Yes</td>
<td align="left">30.8</td>
<td align="left">40.0</td>
<td align="left">2.1</td>
<td align="left">58.8</td>
<td align="left">10.0</td>
<td align="left">8.3</td>
<td align="left">53.8</td>
<td align="left">6.7</td>
<td align="left">2.1</td>
<td align="left">70.8</td>
</tr>
<tr>
<td align="left">No</td>
<td align="left">10.4</td>
<td align="left">18.8</td>
<td align="left">0.4</td>
<td align="left">24.2</td>
<td align="left">4.6</td>
<td align="left">2.1</td>
<td align="left">23.8</td>
<td align="left">1.7</td>
<td align="left">1.7</td>
<td align="left">29.2</td>
</tr>
<tr>
<td align="left">&#x3c7;<sup>2</sup> (<italic>p</italic>-value)</td>
<td align="left">&#x200b;</td>
<td align="left">
<bold>0.330</bold>
</td>
<td align="left">&#x200b;</td>
<td align="left">&#x200b;</td>
<td align="left">
<bold>0.304</bold>
</td>
<td align="left">&#x200b;</td>
<td align="left">&#x200b;</td>
<td align="left">&#x200b;</td>
<td align="left">
<bold>0.023</bold>
</td>
<td align="left">&#x200b;</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn>
<p>&#x3c7;2 &#x3d; chi-square test; <italic>p</italic> &#x2264; 0.05 shows a significant difference.</p>
</fn>
<fn>
<p>Source: Field survey.</p>
</fn>
<fn>
<p>The bold values show the significance difference based on Chi-square (p-values) between the groups of sex, age, and education level.</p>
</fn>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="s3-2-2">
<label>3.2.2</label>
<title>Maize seed cultivar used</title>
<p>Across the study districts, farmers reported using both local and improved maize varieties. The majority (41.2%) planted landrace maize cultivars. The respondents&#x2019; education level has a significant (<italic>p</italic> &#x3d; 0.02549) influence on the choice of maize variety (<xref ref-type="table" rid="T3">Table 3</xref>). Hence, many responders with primary education (30%) chose the landrace maize cultivar compared to those with tertiary education (0.4%).</p>
</sec>
<sec id="s3-2-3">
<label>3.2.3</label>
<title>Maize seed recycling practices</title>
<p>Seed recycling is common practice among maize farmers in the surveyed area (<xref ref-type="table" rid="T3">Table 3</xref>), with 70.8% of farmers reporting recycling maize seed from their previous harvests. The respondents&#x2019; education level significantly influenced farmers&#x2019; decision to recycle maize seed (<italic>p</italic> &#x3d; 0.02349). Most respondents with primary education (53.8%) opt to recycle their maize seed, compared with 2.1% with tertiary education.</p>
</sec>
<sec id="s3-2-4">
<label>3.2.4</label>
<title>Motive for maize seed recycling</title>
<p>The majority of the respondents (91.8%) cited financial constraint as the primary reason for recycling maize seeds (<xref ref-type="table" rid="T4">Table 4</xref>). However, sex, age, and education have no significant influence (<italic>p</italic> &#x3d; 0.942, 0.2469, and 0.5067, respectively) on the motive for the maize seed recycling practice among farmers. The associations between farmers&#x2019; demographic characteristics and motives for maize seed recycling were assessed using Pearson&#x2019;s chi-square test of independence.</p>
<table-wrap id="T4" position="float">
<label>TABLE 4</label>
<caption>
<p>Motives for maize seed recycling according to farmers&#x2019; characteristics.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th rowspan="2" align="left">Motive for maize seed recycling</th>
<th colspan="2" align="left">Sex (%)</th>
<th colspan="3" align="left">Age (%)</th>
<th colspan="4" align="left">Education (%)</th>
<th align="left">Total (%)</th>
</tr>
<tr>
<th align="left">F</th>
<th align="left">M</th>
<th align="left">Young (19&#x2013;25)</th>
<th align="left">Adult (26&#x2013;59)</th>
<th align="left">Elder (&#x2265;60)</th>
<th align="left">No formal education</th>
<th align="left">Primary</th>
<th align="left">Secondary</th>
<th align="left">Tertiary</th>
<th align="left">Total (%)</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td colspan="11" align="center">Farmers&#x2019; count and significance based on demographic characteristics (n &#x3d; 170)</td>
</tr>
<tr>
<td align="left">Financial constraint</td>
<td align="left">39.4</td>
<td align="left">52.4</td>
<td align="left">2.4</td>
<td align="left">77.1</td>
<td align="left">12.4</td>
<td align="left">11.8</td>
<td align="left">68.2</td>
<td align="left">9.4</td>
<td align="left">2.4</td>
<td align="left">91.8</td>
</tr>
<tr>
<td align="left">High yield</td>
<td align="left">1.8</td>
<td align="left">1.2</td>
<td align="left">0.0</td>
<td align="left">1.8</td>
<td align="left">1.2</td>
<td align="left">0.0</td>
<td align="left">2.9</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">2.9</td>
</tr>
<tr>
<td align="left">Higher G%</td>
<td align="left">0.6</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">1.2</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">1.2</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">1.2</td>
</tr>
<tr>
<td align="left">Preference</td>
<td align="left">1.8</td>
<td align="left">2.4</td>
<td align="left">0.6</td>
<td align="left">2.9</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">3.5</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">4.1</td>
</tr>
<tr>
<td align="left">&#x3c7;<sup>2</sup> (P-value)</td>
<td align="left">&#x200b;</td>
<td align="left">
<bold>0.942</bold>
</td>
<td align="left">&#x200b;</td>
<td align="left">&#x200b;</td>
<td align="left">
<bold>0.247</bold>
</td>
<td align="left">&#x200b;</td>
<td align="left">&#x200b;</td>
<td align="left">&#x200b;</td>
<td align="left">
<bold>0.5067</bold>
</td>
<td align="left">&#x200b;</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn>
<p>&#x3c7;2 &#x3d; chi-square test; <italic>p</italic> &#x2264; 0.05 shows a significant difference. Source: Field survey.</p>
</fn>
<fn>
<p>The bold values show the significance difference based on Chi-square (p-values) between the groups of sex, age, and education level.</p>
</fn>
</table-wrap-foot>
</table-wrap>
</sec>
</sec>
<sec id="s3-3">
<label>3.3</label>
<title>Farmer-saved maize seed storage practices</title>
<sec id="s3-3-1">
<label>3.3.1</label>
<title>Packaging materials used for the storage of farmers&#x2019; saved maize seed</title>
<p>Most respondents stored their maize seed after harvest for planting in the following season. The common storage materials used were polypropylene bags (71.2%), followed by hermetic bags (14.7%). Respondents with a primary school education were the majority across all seed storage methods/materials (<xref ref-type="table" rid="T5">Table 5</xref>). Recycled plastic containers, buckets, plastic drums, plastic tins, metal silos, and metal drums were less frequently used by the majority compared to polypropylene and hermetic bags. Traditional methods such as &#x201c;Kihenge,&#x201d; kitchen hanging, and &#x201c;Chanja&#x201d; were among the methods used by farmers.</p>
<table-wrap id="T5" position="float">
<label>TABLE 5</label>
<caption>
<p>Current farmers&#x2019; saved maize seed storage materials in the surveyed area in relation to farmers&#x2019; characteristics.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th rowspan="2" align="left">Storage material</th>
<th colspan="2" align="left">Sex (%)</th>
<th colspan="3" align="left">Age (%)</th>
<th colspan="4" align="left">Education (%)</th>
</tr>
<tr>
<th align="left">M</th>
<th align="left">F</th>
<th align="left">Young (19&#x2013;25)</th>
<th align="left">Adult (26&#x2013;59)</th>
<th align="left">Elder (&#x2265;60)</th>
<th align="left">No formal education</th>
<th align="left">Primary</th>
<th align="left">Secondary</th>
<th align="left">Tertiary</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td colspan="10" align="center">Farmers&#x2019; percentage based on demographic characteristics (n &#x3d; 170)</td>
</tr>
<tr>
<td align="left">Polypropylene</td>
<td align="left">40.6</td>
<td align="left">30.6</td>
<td align="left">2.4</td>
<td align="left">57.1</td>
<td align="left">11.8</td>
<td align="left">8.8</td>
<td align="left">57.6</td>
<td align="left">4.7</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Hermetic bag</td>
<td align="left">9.4</td>
<td align="left">5.3</td>
<td align="left">0.6</td>
<td align="left">12.9</td>
<td align="left">1.2</td>
<td align="left">1.2</td>
<td align="left">8.8</td>
<td align="left">2.4</td>
<td align="left">2.4</td>
</tr>
<tr>
<td align="left">Recycled plastic container</td>
<td align="left">2.4</td>
<td align="left">1.8</td>
<td align="left">0.0</td>
<td align="left">4.1</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">2.4</td>
<td align="left">0.6</td>
<td align="left">0.6</td>
</tr>
<tr>
<td align="left">Plastic drum</td>
<td align="left">0.6</td>
<td align="left">1.8</td>
<td align="left">0.0</td>
<td align="left">2.4</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">1.2</td>
<td align="left">1.2</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Plastic tins</td>
<td align="left">1.2</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">1.2</td>
<td align="left">0.0</td>
<td align="left">1.2</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Metal silo</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Metal drum</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Kihenge</td>
<td align="left">0.6</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Hanging in the kitchen</td>
<td align="left">0.6</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Grain silo</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Chanja</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Bucket</td>
<td align="left">0.6</td>
<td align="left">2.4</td>
<td align="left">0.0</td>
<td align="left">2.9</td>
<td align="left">0.0</td>
<td align="left">0.6</td>
<td align="left">2.4</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Total (%)</td>
<td align="left">56.6</td>
<td align="left">44.9</td>
<td align="left">3.0</td>
<td align="left">81.8</td>
<td align="left">15.4</td>
<td align="left">11.8</td>
<td align="left">76.0</td>
<td align="left">9.5</td>
<td align="left">3.0</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn>
<p>Source: Field survey.</p>
</fn>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="s3-3-2">
<label>3.3.2</label>
<title>Seed treatment of farmers saved maize</title>
<p>The majority of respondents (63.9%) treated their maize seeds with insecticide before storage (<xref ref-type="table" rid="T6">Table 6</xref>). The practice is common among adults (53.5%) and those with primary education (50.7%). Shumba dust pesticide was the most popular treatment cited by (56%) maize farmers, particularly adults (44.8%) with primary education (45.5%).</p>
<table-wrap id="T6" position="float">
<label>TABLE 6</label>
<caption>
<p>Maize seed treatment in the surveyed area in relation to farmers&#x2019; characteristics.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th rowspan="2" align="center">Variable</th>
<th rowspan="2" align="center">Category</th>
<th colspan="2" align="left">Sex (%)</th>
<th colspan="3" align="left">Age (%)</th>
<th colspan="4" align="left">Education (%)</th>
</tr>
<tr>
<th align="left">M</th>
<th align="left">F</th>
<th align="left">Young (19&#x2013;25)</th>
<th align="left">Adult (26&#x2013;59)</th>
<th align="left">Elder (&#x2265;60)</th>
<th align="left">No formal education</th>
<th align="left">Primary</th>
<th align="left">Secondary</th>
<th align="left">Tertiary</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td colspan="11" align="center">Farmers&#x2019; percentage (%) based on demographic characteristics</td>
</tr>
<tr>
<td rowspan="2" align="left">Use of maize seed treatment (N &#x3d; 213)</td>
<td align="left">No</td>
<td align="left">19.7</td>
<td align="left">16.4</td>
<td align="left">20.7</td>
<td align="left">0.0</td>
<td align="left">21.7</td>
<td align="left">0.0</td>
<td align="left">22.7</td>
<td align="left">4.2</td>
<td align="left">0.9</td>
</tr>
<tr>
<td align="left">Yes</td>
<td align="left">37.6</td>
<td align="left">26.3</td>
<td align="left">1.9</td>
<td align="left">53.5</td>
<td align="left">8.5</td>
<td align="left">6.6</td>
<td align="left">50.7</td>
<td align="left">4.7</td>
<td align="left">1.9</td>
</tr>
<tr>
<td rowspan="11" align="left">Treatment used to treat maize seed (n &#x3d; 134)</td>
<td align="left">Shumba dust</td>
<td align="left">33.6</td>
<td align="left">22.4</td>
<td align="left">2.2</td>
<td align="left">44.8</td>
<td align="left">9.0</td>
<td align="left">5.2</td>
<td align="left">45.5</td>
<td align="left">3.0</td>
<td align="left">3.0</td>
</tr>
<tr>
<td align="left">Actellic dust</td>
<td align="left">17.9</td>
<td align="left">11.9</td>
<td align="left">0.7</td>
<td align="left">27.6</td>
<td align="left">1.5</td>
<td align="left">3.0</td>
<td align="left">24.6</td>
<td align="left">1.5</td>
<td align="left">0.7</td>
</tr>
<tr>
<td align="left">Ash</td>
<td align="left">1.5</td>
<td align="left">3.0</td>
<td align="left">0.0</td>
<td align="left">3.7</td>
<td align="left">0.7</td>
<td align="left">1.5</td>
<td align="left">2.2</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Neem leaves&#x2019; powder</td>
<td align="left">3.4</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">2.2</td>
<td align="left">1.5</td>
<td align="left">0.0</td>
<td align="left">3.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Bakila dust</td>
<td align="left">0.7</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">1.5</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">1.5</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Canine dust</td>
<td align="left">0.7</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Actellic (liquid form)</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Threshed material</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Petrol</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Over drying</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
</tr>
<tr>
<td align="left">Sulphur tablets</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
<td align="left">0.7</td>
<td align="left">0.0</td>
<td align="left">0.0</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn>
<p>Source: Field survey.</p>
</fn>
</table-wrap-foot>
</table-wrap>
</sec>
</sec>
<sec id="s3-4">
<label>3.4</label>
<title>Causes of the postharvest losses of farmer-saved maize seed in the storage facility</title>
<p>The first reported common cause of postharvest losses of farmer-saved maize seeds, with the highest percentage score (20.6%), was damage due to the combination of respondents and insects (<xref ref-type="table" rid="T7">Table 7</xref>). The second most common cause of loss of the saved maize seed was insects, with the reported percentage of 19.1%. Among the storage materials assessed, the polypropylene was the most affected by the cause of saved maize seed losses, in terms of percentage values of affected storage materials. This is evident in the only cause (rodents and over-drying) of the saved maize seed loss, which had the lowest percentage value of 0.7% for polypropylene.</p>
<table-wrap id="T7" position="float">
<label>TABLE 7</label>
<caption>
<p>Causes of loss/damage of saved maize seed owned by farmers in the surveyed area.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th align="left">Cause of maize seed losses</th>
<th align="left">Storage material/Facility</th>
<th align="left">Farmers&#x2019; count (n &#x3d; 136)</th>
<th align="left">Percentage (%)</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td align="left">Insects</td>
<td align="left">Polypropylene</td>
<td align="left">26</td>
<td align="left">19.1</td>
</tr>
<tr>
<td align="left">Insects</td>
<td align="left">Hanging in the kitchen</td>
<td align="left">1</td>
<td align="left">0.7</td>
</tr>
<tr>
<td align="left">Insects and over-drying</td>
<td align="left">PICS</td>
<td align="left">1</td>
<td align="left">0.7</td>
</tr>
<tr>
<td align="left">Insects and over-drying</td>
<td align="left">Plastic drum</td>
<td align="left">1</td>
<td align="left">0.7</td>
</tr>
<tr>
<td align="left">Insects and over-drying</td>
<td align="left">Polypropylene</td>
<td align="left">7</td>
<td align="left">5.1</td>
</tr>
<tr>
<td align="left">Insects and rodents</td>
<td align="left">Polypropylene</td>
<td align="left">2</td>
<td align="left">1.5</td>
</tr>
<tr>
<td align="left">Insects and rot</td>
<td align="left">Polypropylene</td>
<td align="left">2</td>
<td align="left">1.5</td>
</tr>
<tr>
<td align="left">Insects, rodents, and over-drying</td>
<td align="left">PICS</td>
<td align="left">1</td>
<td align="left">0.7</td>
</tr>
<tr>
<td align="left">Insects, rodents, and over-drying</td>
<td align="left">Polypropylene</td>
<td align="left">6</td>
<td align="left">4.4</td>
</tr>
<tr>
<td align="left">Insects, rodents, and rot</td>
<td align="left">PICS</td>
<td align="left">1</td>
<td align="left">0.7</td>
</tr>
<tr>
<td align="left">Over-drying</td>
<td align="left">Polypropylene</td>
<td align="left">2</td>
<td align="left">1.5</td>
</tr>
<tr>
<td align="left">Over-drying</td>
<td align="left">PICS</td>
<td align="left">2</td>
<td align="left">1.5</td>
</tr>
<tr>
<td align="left">Rodent and theft</td>
<td align="left">Polypropylene</td>
<td align="left">5</td>
<td align="left">3.7</td>
</tr>
<tr>
<td align="left">Rodent and theft</td>
<td align="left">Polypropylene</td>
<td align="left">2</td>
<td align="left">1.5</td>
</tr>
<tr>
<td align="left">Rodents</td>
<td align="left">Polypropylene</td>
<td align="left">22</td>
<td align="left">16.2</td>
</tr>
<tr>
<td align="left">Rot</td>
<td align="left">PICS</td>
<td align="left">11</td>
<td align="left">8.1</td>
</tr>
<tr>
<td align="left">Rot</td>
<td align="left">Bucket</td>
<td align="left">1</td>
<td align="left">0.7</td>
</tr>
<tr>
<td align="left">Rot</td>
<td align="left">Metal silo</td>
<td align="left">1</td>
<td align="left">0.7</td>
</tr>
<tr>
<td align="left">Rodents and insects</td>
<td align="left">Plastic drum</td>
<td align="left">1</td>
<td align="left">0.7</td>
</tr>
<tr>
<td align="left">Rodents and insects</td>
<td align="left">Polypropylene</td>
<td align="left">2</td>
<td align="left">1.5</td>
</tr>
<tr>
<td align="left">Rodents and insects</td>
<td align="left">Polypropylene</td>
<td align="left">28</td>
<td align="left">20.6</td>
</tr>
<tr>
<td align="left">Rodents and over-drying</td>
<td align="left">Polypropylene</td>
<td align="left">1</td>
<td align="left">0.7</td>
</tr>
<tr>
<td align="left">Rodents, insects, over-drying, and poor handling</td>
<td align="left">Polypropylene</td>
<td align="left">4</td>
<td align="left">2.9</td>
</tr>
<tr>
<td align="left">Rodents and rot</td>
<td align="left">PICS</td>
<td align="left">1</td>
<td align="left">0.7</td>
</tr>
<tr>
<td align="left">Rodents and rot</td>
<td align="left">Polypropylene</td>
<td align="left">3</td>
<td align="left">2.2</td>
</tr>
<tr>
<td align="left">Rodents, insects, and over-drying</td>
<td align="left">Kihenge</td>
<td align="left">1</td>
<td align="left">0.7</td>
</tr>
<tr>
<td align="left">Theft</td>
<td align="left">Polypropylene</td>
<td align="left">1</td>
<td align="left">0.7</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn>
<p>Source: Field survey.</p>
</fn>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="s3-5">
<label>3.5</label>
<title>Quality of stored farmer-saved maize seed</title>
<p>The analysis of laboratory-collected data revealed a significant difference (<italic>p</italic> &#x2264; 0.05) in seed quality parameters, as determined by Tukey&#x2019;s HSD test (<xref ref-type="table" rid="T8">Table 8</xref>). The quality of seeds was influenced by location, storage-packaging materials, and storage time. The parameters considered were moisture content, germination, and vigour, with moisture content and germination expressed as percentages and vigour as an index.</p>
<table-wrap id="T8" position="float">
<label>TABLE 8</label>
<caption>
<p>Effect of location, storage package materials, and storage time on germination (%), vigour index, and moisture content (%) of stored farm-saved maize seeds (n &#x3d; 200 seeds per treatment).</p>
</caption>
<table>
<thead valign="top">
<tr>
<th align="center">Location</th>
<th align="left">Storage material</th>
<th align="left">Moisture content (initial)</th>
<th align="center">Moisture content (AF 3&#xa0;months)</th>
<th align="center">PCMC</th>
<th align="center">Germination % (initial)</th>
<th align="left">Germination% (AF 3&#xa0;months)</th>
<th align="center">PCG%</th>
<th align="left">Vigour index (initial)</th>
<th align="left">Vigour index (AF 3&#xa0;months)</th>
<th align="center">PCVI</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td align="left">Kilosa</td>
<td align="left">Poly</td>
<td align="left">13.967 a</td>
<td align="left">12.67 a</td>
<td align="left">9.29 a</td>
<td align="left">93.00 a</td>
<td align="left">82.0 c</td>
<td align="left">11.84 a</td>
<td align="left">4774 a</td>
<td align="left">3760.67 b</td>
<td align="left">21.29 a</td>
</tr>
<tr>
<td align="left">Kilosa</td>
<td align="left">Poly &#x2b; insecticide</td>
<td align="left">13.03 a</td>
<td align="left">12.50 ab</td>
<td align="left">4.06 b</td>
<td align="left">92.33 a</td>
<td align="left">84.5 b</td>
<td align="left">8.49 b</td>
<td align="left">4709 a</td>
<td align="left">4041.83 ab</td>
<td align="left">14.16 a</td>
</tr>
<tr>
<td align="left">Kilosa</td>
<td align="left">Hermetic</td>
<td align="left">12.90 a</td>
<td align="left">12.20 b</td>
<td align="left">5.43 b</td>
<td align="left">93.33 a</td>
<td align="left">88.0 a</td>
<td align="left">5.70 b</td>
<td align="left">4980 a</td>
<td align="left">4326.00 a</td>
<td align="left">12.88 a</td>
</tr>
<tr>
<td align="left">Kilosa</td>
<td align="left">LSD</td>
<td align="left">0.4034</td>
<td align="left">0.3544</td>
<td align="left">3.7239</td>
<td align="left">1.7721</td>
<td align="left">1.963</td>
<td align="left">2.99</td>
<td align="left">492.52</td>
<td align="left">313.37</td>
<td align="left">9.788</td>
</tr>
<tr>
<td align="left">Mvomero</td>
<td align="left">Poly</td>
<td align="left">13.67 a</td>
<td align="left">12.03 b</td>
<td align="left">11.76 a</td>
<td align="left">92.66 ab</td>
<td align="left">82.5 a</td>
<td align="left">10.96 a</td>
<td align="left">4771.33 b</td>
<td align="left">3719.88 b</td>
<td align="left">21.98 a</td>
</tr>
<tr>
<td align="left">Mvomero</td>
<td align="left">Poly &#x2b; insecticide</td>
<td align="left">13.40 a</td>
<td align="left">12.50 a</td>
<td align="left">6.65 a</td>
<td align="left">91.00&#xa0;b</td>
<td align="left">82.16 a</td>
<td align="left">9.72 a</td>
<td align="left">4793&#xa0;b</td>
<td align="left">3901.66&#xa0;b</td>
<td align="left">18.61 a</td>
</tr>
<tr>
<td align="left">Mvomero</td>
<td align="left">Hermetic</td>
<td align="left">14.00 a</td>
<td align="left">12.43 a</td>
<td align="left">10.9 a</td>
<td align="left">94 a</td>
<td align="left">86.33 a</td>
<td align="left">8.14 a</td>
<td align="left">5045 a</td>
<td align="left">4381.76 a</td>
<td align="left">13.15 a</td>
</tr>
<tr>
<td align="left">Mvomero</td>
<td align="left">LSD</td>
<td align="left">1.371</td>
<td align="left">0.3544</td>
<td align="left">9.614</td>
<td align="left">2.203</td>
<td align="left">7.7845</td>
<td align="left">8.318</td>
<td align="left">249.45</td>
<td align="left">438.48</td>
<td align="left">9.5237</td>
</tr>
<tr>
<td align="left">Gairo</td>
<td align="left">Poly</td>
<td align="left">12.80 a</td>
<td align="left">12.16 a</td>
<td align="left">4.92 a</td>
<td align="left">91.67 a</td>
<td align="left">80.83 b</td>
<td align="left">11.78 a</td>
<td align="left">4582.67 a</td>
<td align="left">3761.91 a</td>
<td align="left">17.84 a</td>
</tr>
<tr>
<td align="left">Gairo</td>
<td align="left">Poly &#x2b; insecticide</td>
<td align="left">12.96 a</td>
<td align="left">11.96 a</td>
<td align="left">7.67 a</td>
<td align="left">93 a</td>
<td align="left">87.66 a</td>
<td align="left">5.75 a</td>
<td align="left">4838.33 a</td>
<td align="left">3961.67 a</td>
<td align="left">18.03 a</td>
</tr>
<tr>
<td align="left">Gairo</td>
<td align="left">Hermetic</td>
<td align="left">12.96 a</td>
<td align="left">12.61 a</td>
<td align="left">6.17 a</td>
<td align="left">93 a</td>
<td align="left">86&#xa0;ab</td>
<td align="left">7.51 a</td>
<td align="left">4962 a</td>
<td align="left">4273.33 a</td>
<td align="left">13.38 a</td>
</tr>
<tr>
<td align="left">Gairo</td>
<td align="left">LSD</td>
<td align="left">0.528</td>
<td align="left">0.6235</td>
<td align="left">6.73</td>
<td align="left">3.022</td>
<td align="left">6.3448</td>
<td align="left">7.267</td>
<td align="left">861.07</td>
<td align="left">548.056</td>
<td align="left">16.806</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn>
<p>The letters a, b, c, and ab show the significance difference based on Tukey&#x0027;s HSD test among the parameters moisture content, germination, and vigour in relation to storage material.</p>
</fn>
</table-wrap-foot>
</table-wrap>
<p>Results indicated a highly significant difference in maize seed moisture content among districts after 3&#xa0;months, as shown in the moisture content column of <xref ref-type="table" rid="T8">Table 8</xref>. In this column, the letter pattern based on the HSD test analysis differs across the three districts. The pattern difference is similar to that observed for germination and vigour. Regarding the types of storage materials that influence the quality of stored seeds, it has been shown that not all materials permit seed rewetting. This is evident in the PCMC column, where all values are positive, and there is no significant difference among them. The lowest moisture content fluctuation, i.e., not more than 8% based on PCMC values, is observed in the poly &#x2b; insecticide. Poly storage had the highest fluctuation in moisture content of all storage types. Hermetic storage showed the highest germination percentage after 3&#xa0;months (88.0% in Kilosa). Additionally, Hermetic had PCG values of 8.2% or less, whereas the other storage types (poly and poly &#x2b; insecticide) exhibited higher reductions in germination (PCG&#x3e; 8.2%). In terms of vigour, hermetic storage showed the lowest percentage change in vigour index (not exceeding 13.4) across all storage types at the three locations. This phenomenon suggests more effective preservation of seed metabolic integrity than Poly and Poly &#x2b; insecticide, as shown in <xref ref-type="table" rid="T8">Table 8</xref> and <xref ref-type="fig" rid="F2">Figure 2</xref>. The high performance of hermetic storage, as reflected in vigour and germination parameters, is attributable to its airtight environment, which reduces oxygen concentration and limits insect and fungal activity, thereby slowing seed respiration and moisture exchange with the external environment. Additionally, the controlled internal atmosphere in hermetic bags likely minimised oxidative damage and enzymatic deterioration, thereby supporting greater seedling vigour and viability than conventional storage.</p>
<fig id="F2" position="float">
<label>FIGURE 2</label>
<caption>
<p>Effect of storage package materials, storage time, and location on the germination percentage and vigour index of farmer-saved maize seeds.</p>
</caption>
<graphic xlink:href="frfst-05-1667337-g002.tif">
<alt-text content-type="machine-generated">Grouped bar charts compare initial and three-month germination percentages and seed vigour indices for Kilosa, Mvomero, and Gairo. Three storage methods are evaluated: Poly, Poly plus Insecticide, and Hermetic. Bars show higher values for hermetic storage after three months, while poly methods display greater declines. Each panel includes two colored bars for each method, labeled as initial and after three months.</alt-text>
</graphic>
</fig>
</sec>
</sec>
<sec sec-type="discussion" id="s4">
<label>4</label>
<title>Discussion</title>
<sec id="s4-1">
<label>4.1</label>
<title>Demographic characteristics</title>
<sec id="s4-1-1">
<label>4.1.1</label>
<title>Sex of respondents</title>
<p>Male respondents accounted for 58.8% of the total participants, indicating that men are more actively engaged in farming activities across the study areas. This pattern may be attributed to traditional gender roles in which men own land and make major agricultural decisions, while women typically participate in production and postharvest activities. <xref ref-type="bibr" rid="B13">Ifie et al. (2022)</xref> reported similar findings, noting that a higher percentage of male individuals (74%) in Ghana were involved in maize production. It shows that women may have limited access to resources, training, and decision-making opportunities in maize production. Policies and programmes should, therefore, target gender inclusivity, providing women with equitable access to land, credit, extension services, and modern agricultural technologies to enhance their contribution to productivity and strengthen household food security.</p>
</sec>
<sec id="s4-1-2">
<label>4.1.2</label>
<title>Age of respondents</title>
<p>A large percentage (82.8%) of farmers were adults, suggesting that most were in their productive years and had greater physical capacity to participate in agricultural activities. The elder farmers (14.2%) aged 60&#xa0;years showed continued engagement of older generations in maize production, which is crucial for knowledge transmission and the preservation of traditional agricultural techniques. The low percentage of young people (2.5%) suggests a possible problem with youth involvement in agriculture. This might be related to youth migration to cities in search of better prospects or to those uninterested in farming because of its perceived difficulties and poor profitability. The lower youth participation threatens the long-term sustainability of smallholder maize production and the transfer of indigenous knowledge. Policies that incentivise youth involvement through access to land, credit, training, and modern technologies are critical to attract younger generations and ensure the continuity and resilience of agricultural systems. <xref ref-type="bibr" rid="B9">Consentino et al. (2023)</xref> documented similar situations and proposed that although youth acknowledge the economic significance of agriculture in their community, they do not view it as their first career option.</p>
</sec>
<sec id="s4-1-3">
<label>4.1.3</label>
<title>Educational level</title>
<p>The educational achievement of farmers in the study area shows that the majority (77.5%) had primary education, which can help them adopt new agricultural technologies and practices. However, 10.4% of farmers had no formal education, which can cause a challenge in the dissemination of advanced storage techniques and knowledge. Although 8.3% have secondary education and 3.8% have tertiary education, these farmers are in a better position to adopt innovative farming practices and contribute to higher productivity. Higher levels of education are linked to greater awareness, adoption of improved seed and storage technologies, and overall farming efficiency. Therefore, targeted training programmes and extension services should prioritise farmers with limited education to bridge knowledge gaps and enhance equitable access to modern agricultural innovations. These findings are consistent with the study by <xref ref-type="bibr" rid="B32">Utonga (2022)</xref>, which reported that 87.5%, 10.8%, and 1.7% of farmers had completed their primary, secondary, and higher education, respectively.</p>
</sec>
</sec>
<sec id="s4-2">
<label>4.2</label>
<title>Status of the maize seed system and farmers&#x2019; saved seed practices in the surveyed area</title>
<sec id="s4-2-1">
<label>4.2.1</label>
<title>Maize seed system used</title>
<p>The majority of farmers (56.2%) depend on informal maize seed systems, while 43.8% use formal systems. This tendency is common in developing countries due to accessibility, affordability, and trust in traditional practices as the dominance of informal systems indicates limited market access and financial capacity among smallholder farmers. Strengthening linkages between formal and informal systems, expanding extension services, and providing financial support through subsidies or credit programmes could help farmers transition toward the use of quality-certified seed while preserving the adaptive strengths of local varieties. This tendency aligns with the findings of <xref ref-type="bibr" rid="B37">Haug et al. (2023)</xref>, who reported that informal seed systems are cost-effective. Furthermore, repeated seed recycling was common, which may contribute to declines in seed vigour and yield performance over time. Education significantly influenced the seed system option (<italic>p</italic> &#x3d; 0.004498). Farmers with higher levels of education were more likely to use formal systems because they were aware of the advantages of certified seed. This aligns with the findings of <xref ref-type="bibr" rid="B21">Mutanyagwa et al. (2018)</xref>, who reported that maize farmers&#x2019; readiness to use improved maize seed increases with higher levels of education, which makes them more open to learning and applying new techniques.</p>
</sec>
<sec id="s4-2-2">
<label>4.2.2</label>
<title>Type of maize cultivar used</title>
<p>Landrace cultivars were common among maize farmers (41.2%) in the surveyed area; they are often preferred for their adaptability to local conditions and cultural significance (<xref ref-type="bibr" rid="B37">Haug et al., 2023</xref>). However, the remarkable utilisation of hybrid seeds (37.6%) indicates awareness of and adoption of their potential for higher yields, reflecting the ongoing efforts of the Tanzanian government and NGOs to promote improved seed varieties (<xref ref-type="bibr" rid="B40">USAID, 2016</xref>). The farmers (21.25%) who use OPV seeds show a moderate preference for seeds that can be recycled without losing yield potential, thereby occupying an intermediate position between traditional and fully hybrid systems. Education plays a significant role (<italic>p</italic> &#x3d; 0.02549) in the choice of maize cultivar, suggesting that educated farmers are more likely to explore different seed options. This is consistent with the findings of <xref ref-type="bibr" rid="B21">Mutanyagwa et al. (2018)</xref>, who reported that the use of improved maize seed increases with higher levels of education, which makes farmers more open to learning and applying new techniques. These results carry important socioeconomic and policy implications, as the coexistence of landrace, OPV, and hybrid varieties reflects both economic diversity and differing levels of access to agricultural innovation. Strengthening extension services, promoting affordable access to hybrid seed, and supporting on-farm conservation of landraces can ensure that introducing improved seeds does not harm local varieties or smallholder farmers&#x2019; capacity to manage risks.</p>
</sec>
<sec id="s4-2-3">
<label>4.2.3</label>
<title>Maize seed recycling</title>
<p>The results reveal that seed recycling is common among maize farmers (70.8%). This aligns with traditional Tanzanian agricultural practices, driven by economic constraints, preferences, and the adaptability of recycled seeds to local conditions (<xref ref-type="bibr" rid="B37">Haug et al., 2023</xref>). This study is among the first to quantitatively assess maize seed recycling in these districts while linking farmer education to recycling behaviour, providing new insights into local seed systems. Farmers with primary education were significantly more likely to recycle seeds (<italic>p</italic> &#x3d; 0.02349), suggesting that traditional practices remain influential even in the context of extension programmes and government initiatives promoting improved varieties. The persistence of seed recycling reflects not only financial hardship but also limited awareness of the long-term benefits of using quality seed. Strengthening farmer education programmes, expanding access to affordable certified seed through cooperatives, and offering targeted subsidies or credit facilities could enhance the adoption of improved seed while maintaining the resilience of local seed systems. On the other hand, 29.2% of farmers who do not recycle seeds show a growing shift toward improved seed varieties, probably influenced by government initiatives and agricultural extension services. This aligns with the findings of <xref ref-type="bibr" rid="B21">Mutanyagwa et al. (2018)</xref>, who reported that maize farmers&#x2019; willingness to use improved maize varieties increases with greater knowledge, making them more flexible in learning and applying new techniques.</p>
</sec>
<sec id="s4-2-4">
<label>4.2.4</label>
<title>Motive of maize seed recycling</title>
<p>Financial constraints were the main reason for seed recycling among maize farmers (91.8%), while preference (4.1%), high yield (2.9%), and higher germination percentage (1.2%) were lesser factors. The <italic>p</italic>-values for sex (<italic>p</italic> &#x3d; 0.942), age (<italic>p</italic> &#x3d; 0.2469), and education (<italic>p</italic> &#x3d; 0.5067) indicate that these factors do not significantly influence seed recycling practices among farmers. This proposes that financial challenges are common problems affecting all demographic groups equally, rather than being determined by differences in gender, age, or educational level. This finding is consistent with the findings of <xref ref-type="bibr" rid="B10">D&#xed;az et al. (2022)</xref>, who argue that financial constraints often drive agricultural practices; however, economic factors, such as income from agriculture, also play a significant role in the adoption of sustainable farming practices. The widespread financial barriers not only perpetuate the use of low-quality recycled seed but also threaten the sustainability and productivity of the seed system. Therefore, interventions such as seed subsidies, access to microcredit, and strengthening of farmer cooperatives are essential to support farmers&#x2019; transition toward formal seed systems. Economically empowering smallholders can enhance their capacity to invest in quality seed, thereby improving yields, food security, and household income stability.</p>
</sec>
</sec>
<sec id="s4-3">
<label>4.3</label>
<title>Farmers&#x2019; saved maize seed storage practice</title>
<sec id="s4-3-1">
<label>4.3.1</label>
<title>Materials used for the storage of farmers&#x2019; saved maize seed</title>
<p>The majority of farmers stored maize seed using polypropylene bags (71.2%). This broad adoption may be due to the affordability and availability of polypropylene bags, which make them ideal for smallholder farmers to store agricultural products. A similar result was reported by <xref ref-type="bibr" rid="B15">Kebede et al. (2024)</xref>, who found that in Ethiopia, traditional storage methods such as polypropylene bags, jute sacks, and typical gombisa facilities are commonly used to store maize for long periods. Although these materials are cheaper and readily available to farmers, their limited protective capacity contributes to higher postharvest losses, reducing household income and undermining the long-term sustainability of local seed systems. Therefore, policies that promote access to affordable hermetic technologies or support cooperative-based storage infrastructure could help strengthen farmer livelihoods and improve seed quality management.</p>
<p>The use of hermetic bags (pics) was less frequent, ranking second-most used (14.7%) after polypropylene bags. Hermetic bags are valued for their ability to prevent pest infestations without chemical inputs, which may explain their moderate popularity, particularly among individuals with basic education who are more aware of the benefits of postharvest management. However, their higher cost compared to polypropylene restricts their wider adoption. These results align with those of <xref ref-type="bibr" rid="B15">Kebede et al. (2024)</xref>, who reported that although hermetic storage methods have been developed and implemented in Ethiopia, smallholder farmers have yet to widely embrace these technologies and continue to rely on conventional storage practices.</p>
<p>Storage materials such as recycled plastic containers, buckets, and plastic drums are less common; this may be due to their high cost and limited durability in protecting produce from insect pests. The minimal use of metal silos, metal drums, and traditional methods, such as &#x201c;Kihenge&#x201d; and hanging in the kitchen, suggests moving away from traditional or more expensive options. Though metal silos and drums are effective in pest control, their higher initial cost limits their adoption by smallholder farmers with tight budgets. Traditional storage methods are also uncommon because modern storage materials offer better preservation. <xref ref-type="bibr" rid="B29">Tibaingana et al. (2018)</xref> emphasised the need for affordable, effective storage solutions that help smallholder farmers improve household-level maize storage.</p>
</sec>
<sec id="s4-3-2">
<label>4.3.2</label>
<title>Farmer saved maize seed treatment</title>
<p>The results reveal that 63.9% of farmers use maize seed treatments, indicating strong awareness of pest and disease protection. The most used insecticide was Shumba dust (56.0%), followed by Actellic dust (29.9%). Though chemical pesticides can cause potential environmental and health risks (<xref ref-type="bibr" rid="B3">Ahmad et al., 2024</xref>). Traditional treatments such as ash (4.5%) and neem leaves (3.7%) are less common but highlight a preference for sustainable practices. According to <xref ref-type="bibr" rid="B23">Ngegba et al. (2022)</xref>, the use of botanical pesticides and other eco-friendly methods can significantly benefit smallholder farmers. There is a remarkable gender inconsistency, with 37.6% of male farmers using treatments compared to 26.3% of female farmers. This could be due to differences in access to resources, training, or roles in agricultural decision-making. Seed treatment is common among adults (53.5%) compared with young (1.9%) and older farmers (8.5%). This may be due to barriers to training or resources that limit adoption among both younger and older farmers. Education also influences adoption: 50.7% of farmers with primary education use seed treatments, compared with 22.7% of those with no formal education. This aligns with the findings of <xref ref-type="bibr" rid="B21">Mutanyagwa et al. (2018)</xref>, who reported that a high level of knowledge makes farmers more open to learning and applying new techniques.</p>
<p>The ability to access and adopt safe and effective seed treatment methods directly influences productivity, income stability, and overall seed system sustainability. Therefore, agricultural policies should promote inclusive training programmes for women, youth, and less educated farmers while encouraging the use of environmentally friendly seed protection techniques to safeguard livelihoods and ecological health.</p>
</sec>
<sec id="s4-3-3">
<label>4.3.3</label>
<title>Causes of postharvest losses of farmer-saved maize seed</title>
<p>The results show that insects are the leading cause of maize seed losses. Insects affect 19.1% of farmers who use polypropylene bags for storage. This aligns with (<xref ref-type="bibr" rid="B24">Pathipati et al., 2021</xref>), who note that insects in stored-grain environments cause significant damage, thereby reducing seed quality and germination. Despite their popularity, polypropylene bags provide limited protection for stored products, resulting in significant post-harvest losses. Similar results were reported by <xref ref-type="bibr" rid="B15">Kebede et al. (2024)</xref>. Another major cause of postharvest maize seed loss is rodent damage (16.2%), which affects farmers. Rodents not only consume stored seeds but also contaminate them and further degrade the quality (<xref ref-type="bibr" rid="B34">Witmer, 2022</xref>). The combined effects of insects and rodents result in a 20.6% loss of maize seed. This combined effect demonstrates the vulnerability of commonly used storage materials when no additional pest control measures are employed.</p>
<p>Maize seed rotting causes 5.9% losses in PICS bags, with smaller losses in metal silos and plastic drums. Temperature, moisture, and fungal contamination are factors that influence the development of rot. Therefore, proper drying before storage is an important consideration (<xref ref-type="bibr" rid="B30">Tsedaley and Adugna, 2016</xref>). Moreover, over-drying results in a 3.7% loss in polypropylene bags, indicating moisture-management challenges (<xref ref-type="bibr" rid="B14">Juki&#x107; et al., 2024</xref>). Theft was reported to account for a 1.5% loss among farmers, indicating that non-pest-related factors also contribute to maize seed postharvest loss.</p>
<p>The persistence of storage-related losses highlights the need for integrated pest management and improved storage infrastructure. Policymakers should prioritise the promotion of hermetic and rodent-proof storage technologies through subsidies, cooperative-based distribution, and capacity-building programmes. Strengthening these systems will not only minimise seed losses but also contribute to the long-term sustainability and resilience of community-based seed systems.</p>
</sec>
</sec>
<sec id="s4-4">
<label>4.4</label>
<title>Quality of the stored farmers&#x2019; saved maize seed</title>
<sec id="s4-4-1">
<label>4.4.1</label>
<title>Germination%, vigour index, and moisture content</title>
<p>Hermetic storage demonstrated superior capacity in maintaining seed germination percentages and vigour across all three locations. This could be due to the preservation of physical characteristics from hermetic storage. By sealing the seeds hermetically, the exchange of moisture and gases with the external environment is prohibited, hence reducing the rate of seed quality deterioration. This result is consistent with the findings of <xref ref-type="bibr" rid="B27">Shahein and Shalaby (2021)</xref>. In contrast, seeds stored in porous materials such as polypropylene bags, whether with or without insecticide, exhibited lower germination rates and vigour. This deterioration is due to the porous nature of these bags, which allows fluctuations in moisture and oxygen levels, creating conditions that promote insect infestations and fungal growth. These findings align with those of <xref ref-type="bibr" rid="B16">Kumari et al. (2017)</xref> and <xref ref-type="bibr" rid="B2">Afzal et al. (2017)</xref>, who also reported the reduced seed quality in non-hermetic storage. Additionally, although initial moisture content was high across all storage types, fluctuations became more pronounced over time. Variability in moisture retention during hermetic storage may be due to frequent opening and closing of these storage materials, whereas porous storage materials, such as polypropylene bags, showed the most pronounced fluctuations, accelerating seed deterioration and fungal growth, as reported by <xref ref-type="bibr" rid="B17">Likhayo et al. (2018)</xref>.</p>
<p>Beyond these physiological mechanisms, the findings carry important socioeconomic and policy implications. Seed quality directly influences crop productivity, household food security, and income stability. When farmers store maize seed under suboptimal conditions, they risk using low-vigour or poorly germinating seed, which can result in uneven crop stands, reduced yields, and financial losses. Conversely, improved storage methods, such as hermetic technology, can enhance seed longevity and performance, enabling farmers to save high-quality seed for subsequent planting seasons and reduce their reliance on frequent seed purchases. This promotes self-reliance and resilience among smallholder farmers, who constitute the backbone of rural economies.</p>
<p>However, the adoption of improved storage technologies is often constrained by economic barriers, including the initial cost of hermetic bags and limited access to information and training. Many smallholder farmers rely on inexpensive, locally available materials such as poly bags despite their poor performance. Therefore, extension programmes and government interventions should focus on raising awareness of the benefits of hermetic storage and subsidising or promoting affordable access to such technologies. Integrating these approaches into national seed system strategies would strengthen both seed quality assurance and the sustainability of local seed systems.</p>
</sec>
</sec>
<sec id="s4-5">
<label>4.5</label>
<title>Study limitations and future work</title>
<p>This study evaluated seed storage for only 3&#xa0;months, which may not capture long-term changes in seed quality. The study was also limited to three districts within the Morogoro region, restricting the generalisability of the results to other areas or agroecological zones. Moreover, the study focused primarily on technical aspects of seed storage, with limited attention to economic outcomes. Future research should involve longer-term monitoring and incorporate market-oriented analyses to better understand the broader impacts on farmer livelihoods.</p>
</sec>
<sec id="s4-6">
<label>4.6</label>
<title>Conclusion and recommendations</title>
<p>The study revealed valuable insights into maize seed systems, storage practices, and the quality of farmers&#x2019; saved maize seed in Kilosa, Gairo, and Mvomero districts. Many farmers (56.2%) depend on informal seed systems due to their accessibility and affordability, a trend more common among farmers with lower levels of education. Therefore, extension services and training programmes should focus on promoting formal seed systems, particularly targeting less-educated farmers. Financial support and subsidies are also essential to make certified seeds more affordable and accessible, thereby bridging the gap and improving maize productivity. Seed recycling remains a common practice, with 70.8% of farmers reusing their maize seeds, mainly due to financial limitations. To address this, targeted financial support programmes or subsidies should be introduced to reduce dependence on recycled seed and enhance overall seed quality and maize productivity.</p>
<p>Awareness of seed treatment is growing, as 63.9% of farmers treat seeds to protect against pests. Shumba dust (56%) and actellic dust (29.9%) are the most widely used treatments, though a smaller proportion of farmers still use traditional methods such as ash and neem leaves. This highlights the need to promote sustainable and eco-friendly seed treatment options. For storage, most farmers use polypropylene bags because they are affordable; however, they offer limited pest protection. Hermetic bags (PICS bags) provide superior pest protection but are less commonly used due to their higher cost. Increasing farmers&#x2019; access to improved storage materials, through subsidies or community-level supply initiatives, could reduce postharvest losses from insects and rodents.</p>
<p>Regarding seed quality, hermetic storage materials, such as PICS bags, outperformed porous materials, such as polypropylene bags. Seeds stored in hermetic conditions had higher germination rates and vigour. In contrast, seeds stored in porous storage, such as polypropylene bags, allowed moisture fluctuations, leading to lower seed quality and higher susceptibility to pests and fungi. Therefore, promoting hermetic storage technologies among smallholder farmers can significantly enhance seed quality, reduce losses, and ultimately improve maize productivity. From a socioeconomic and policy perspective, improved seed and storage practices have significant implications for smallholder livelihoods. Enhancing access to quality seeds and effective storage not only reduces postharvest losses but also strengthens seed system sustainability, increases food security, and can improve household income. Policies that support training, subsidies, and community-based seed and storage programmes are essential to facilitate adoption and ensure that technological improvements benefit all farmers, including those with limited resources.</p>
</sec>
</sec>
</body>
<back>
<sec sec-type="data-availability" id="s5">
<title>Data availability statement</title>
<p>The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.</p>
</sec>
<sec sec-type="ethics-statement" id="s6">
<title>Ethics statement</title>
<p>The studies involving humans were approved by the Sokoine University of Agriculture. The studies were conducted in accordance with the local legislation and institutional requirements. Written informed consent for participation was not required from the participants or the participants&#x2019; legal guardians/next of kin because verbal (not written) informed consent for participation was obtained from the participant due to limited literacy in the study area.</p>
</sec>
<sec sec-type="author-contributions" id="s7">
<title>Author contributions</title>
<p>MM: Project administration, Formal analysis, Methodology, Visualization, Conceptualization, Writing &#x2013; original draft, Writing &#x2013; review and editing, Resources, Funding acquisition, Investigation, Data curation. YY: Visualization, Writing &#x2013; review and editing, Conceptualization, Supervision. RM: Data curation, Conceptualization, Writing &#x2013; review and editing, Supervision.</p>
</sec>
<sec sec-type="COI-statement" id="s9">
<title>Conflict of interest</title>
<p>The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec sec-type="ai-statement" id="s10">
<title>Generative AI statement</title>
<p>The author(s) declared that generative AI was not used in the creation of this manuscript.</p>
<p>Any alternative text (alt text) provided alongside figures in this article has been generated by Frontiers with the support of artificial intelligence and reasonable efforts have been made to ensure accuracy, including review by the authors wherever possible. If you identify any issues, please contact us.</p>
</sec>
<sec sec-type="disclaimer" id="s11">
<title>Publisher&#x2019;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
<fn-group>
<fn fn-type="custom" custom-type="edited-by">
<p>
<bold>Edited by:</bold> <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/3190352/overview">Xudong Zhu</ext-link>, Fujian Academy of Agricultural Sciences, China</p>
</fn>
<fn fn-type="custom" custom-type="reviewed-by">
<p>
<bold>Reviewed by:</bold> <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/182206/overview">Vodjo Nicod&#xe8;me Fassinou Hotegni</ext-link>, University of Abomey-Calavi, Benin</p>
<p>
<ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/3246172/overview">Hija Mwatawala</ext-link>, Institute of Rural Development Planning, Tanzania</p>
</fn>
</fn-group>
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