AUTHOR=Linga BalaSubramani Gattu , Ibrahim Faisal E. , Razzaq Aleem , Samara Muthanna , Roshanuddin Jameela , Adi Hind H. , Al-Dewik Aseel , Ahmedoglu Ayla J. , Hamdan Rand , Ahmed Amal E. I. , Qoronfleh M. Walid , Zayed Hatem , Elshiekh Duaa , Ellaithi Mona , Alsharshani Mohamed , Abdulrouf Palli Valapila , Farrell Thomas , Kurdi Bader , Abdoh Ghassan , Al Rifai Hilal , Al-Dewik Nader 

TITLE=Epigenetic profiling of preterm birth: a dual-tissue methylation patterns using long-read sequencing

JOURNAL=Frontiers in Epigenetics and Epigenomics

VOLUME=Volume 3 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/epigenetics-and-epigenomics/articles/10.3389/freae.2025.1644521

DOI=10.3389/freae.2025.1644521

ISSN=2813-706X

ABSTRACT=IntroductionPreterm birth (PTB), a leading cause of neonatal morbidity and mortality, arises from complex maternal-fetal interactions with multifactorial origins. Emerging evidence suggests that epigenetic dysregulation may mediate these interactions. This study aimed to identify DNA methylation changes associated with PTB to uncover potential biomarkers and underlying mechanisms.MethodsWe employed long-read sequencing to profile genome-wide DNA methylation followed by gene ontology and pathway enrichment analysis in matched maternal peripheral blood and neonatal cord blood from 15 preterm and 7 full-term deliveries (mother–infant pairs).ResultsA total of 1,151 significantly differentially methylated regions (DMRs) and 25,336 differentially methylated loci (DMLs) were identified across maternal and neonatal blood samples. In maternal blood from PTB cases, the most significantly hypermethylated genes were MED38, PSMB11, and WNT7B, whereas EXTL3 and MMP9 were among the most hypomethylated. Additionally, the promoters of VWA5A, EIF4E3, ZNF571, and COPB2 exhibited significant hypermethylation, while those of SIRPB1 and TNFRSF19 showed hypomethylation. In neonatal cord blood from PTB cases, the most significantly hypermethylated genes were LOC401478, ISG20, LMTK3, TCAF2, and COL4A2, whereas EXTL3 and MMP9 were among the most hypomethylated. Promoters of DKK3, CELF2, and IFI35 were notably hypermethylated, whereas ALOX12 and CLBA1 were among the most hypomethylated. Enrichment analysis revealed that these epigenetic alterations impact critical developmental, immune, and neuroendocrine pathways, including Wnt signaling, calcium signaling, MAPK, oxytocin signaling, and neuroactive ligand-receptor interaction. Comparative analysis identified 120 overlapping DMLs, with 91 hypermethylated and 28 hypomethylated consistently across maternal and neonatal samples, including DPPA3, ABCA1, and GKN1. In contrast, 20,240 and 4,770 DMLs were unique to cord and peripheral blood, respectively. Additionally, 14 overlapping DMRs were mapped to genes such as PLD5, FBXO40, GMNC, HHIP, CLEC18B, and LHX1, exhibiting non-random chromosomal clustering. Enrichment analysis of these shared DMRs revealed significant involvement in developmental processes, including skeletal morphogenesis, axis patterning, and fibroblast growth factor signaling, indicating convergence on core regulatory pathways in PTB.ConclusionThis is the first dual-tissue PTB study using long-read methylation profiling. Our results reveal distinct and shared epigenetic signatures in maternal and neonatal compartments, offering insights into the molecular etiology of PTB and potential biomarkers for early detection and therapeutic intervention.