Front. Environ. Sci. Frontiers in Environmental Science Front. Environ. Sci. 2296-665X Frontiers Media S.A. 1204787 10.3389/fenvs.2023.1204787 Environmental Science Original Research Molecular, morphological and ecological studies of Limnospira platensis (Cyanobacteria), from saline and alkaline lakes, Pantanal Biome, Brazil Santos et al. 10.3389/fenvs.2023.1204787 Santos Kleber Renan de Souza 1 2 * † Hentschke Guilherme Scotta 3 * † Ferrari Graciela 4 Andreote Ana Paula Dini 5 Fiore Marli de Fátima 5 Vasconcelos Vitor 3 6 Sant’Anna Célia Leite 7 1 Centro Universitário Leonardo da Vinci—Uniasselvi, Indaial, Brazil 2 Fundação Universidade Regional de Blumenau (FURB), Blumenau, Brazil 3 Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Blue Biotechnology and Ecotoxicology Laboratory, Matosinhos, Portugal 4 Laboratorio Tecnologico del Uruguay (LATU), Montevideo, Uruguay 5 Centro de Energia Nuclear na Agricultura (CENA), Universidade de São Paulo (USP), Piracicaba, Brazil 6 Faculdade de Ciências, Universidade do Porto, Porto, Portugal 7 Instituto de Pesquisas Ambientais (IPA), São Paulo, Brazil

Edited by: Allan Douglas Cembella, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research (AWI), Germany

Reviewed by: Prashant Kumar Singh, Mizoram University, India

Itzel Becerra-Absalón, National Autonomous University of Mexico, Mexico

 *Correspondence: Guilherme Scotta Hentschke, guilherme.scotta@gmail.com; Kleber Renan de Souza Santos, santoskrs@gmail.com

These authors share first authorship

 25 09 2023 2023 11 1204787 12 04 2023 07 09 2023 Copyright © 2023 Santos, Hentschke, Ferrari, Andreote, Fiore, Vasconcelos and Sant’Anna. 2023 Santos, Hentschke, Ferrari, Andreote, Fiore, Vasconcelos and Sant’Anna

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We studied nineteen populations of A. platensis (Microcoleaceae, Cyanobacteria), from Pantanal (Brazil) shallow, saline and alkaline lakes. The lakes are connected to the Paraná and Paraguay Rivers Basins, and during wet seasons, the waters flow towards La Plata River estuary. Morphology of natural populations and cultures were analyzed using optical microscope, and 16S rDNA sequences were used for the BI and ML phylogenetic analysis. The morphological analysis shows that our populations fit in the original description of A. platensis, but with additional aerotopes. Also, it is evident that these structures are facultative and the species is planktonic, rather than benthic without aerotopes, as originally described. The phylogenetic analysis shows our strains in the monophyletic Limnospira clade. Considering that, in this paper we transfer the species A. platensis to the genus Limnospira, based on phylogenetic and morphological data. This new taxonomical combination is supported also by ecological data, and indicates that the species is more related and abundant in Pantanal, than in La Plata Basin, region from where it was originally described. According to our results, L. platensis is planktonic or benthic and typical from saline, alkaline and warm waters.

 salines extreme environments biodiversity polyphasic approach new combination section-at-acceptance Freshwater Science 1 Introduction

Arthrospira platensis (Nodst.) Gomont is a Cyanobacteria largely used in industrial scale, as a food supplement, under the commercial name “Spirulina”. Although it is one of the most studied Cyanobacteria, due to its economic importance, its taxonomy is still controversial (Nowicka-Krawczyk et al., 2019). In an important taxonomic revision of the genus Arthrospira (Komárek and Lund, 1990), the authors included Spirulina maxima and S. fusiformis in the planktonic forms (with aerotopes) of Arthrospira, as A. maxima Setchell et Gardner and A. fusiformis (Voronichin) Komárek et Lund. Based on an exsiccate of the type material of A. platensis, held at the British Museum of Natural History, the authors considered this species as benthic, without aerotopes. Some authors disagree and argue that aerotopes are lost in dry material (Tomaselli, 1997) and that the Gomont iconotype does not show all the morphological variability of the species. Because of that, A. platensis hypothetically could present aerotopes and be planktonic.

In this paper, we extensively studied nineteen A. platensis populations from saline and alkaline lakes of Pantanal da Nhecolândia, Brazil. The Pantanal Biome is located in the center of South America and is the greatest wetland in the planet (Allem and Valls, 1987). In the sub-region Pantanal da Nhecolândia, the presence of thousands of shallow lakes (maximum 2 m depth), predominantly rounded are remarkable. These lakes are commonly dominated by cyanobacterial blooms of A. platensis and Anabaenopsis elenkinii Miller. The populations of A. platensis in our samples, fit in the original description of the species, but present facultative aerotopes and are phylogenetic related to the genus Limnospira Nowicka-Krawczyk et al. Consequently, based on phylogenetic, morphological and ecological data, in this paper we propose to transfer A. platensis to the genus Limnospira.

 2 Materials and methods 2.1 Sampling and physicochemical water parameters

Nineteen water samples were collected in dry and wet seasons from 2004 to 2012, by immersing bottles, in “Pantanal da Nhecolândia”, Brazil, lakes. Located between Paraná and Paraguay Rivers, the region is characterized by the presence of thousands of shallow lakes (Figure 1) with alkaline (pH 9–11) and saline waters with high conductivity (>2000 μS cm-1), dominated by the Cyanobacteria A. elenkinii and “A. platensis”. The lakes are part of the Paraguay River Basin, which is composed by the Paraguay-Paraná-Plata Rivers systems. During the wet seasons, the lakes are connected to this system and the waters flow towards La Plata River estuary (Santos and Sant’Anna, 2010).

(A) General view of Pantanal da Nhecolândia lakes, (B) Salina do Meio in Nhumirim Farm (Credits: (A). A.Y. Sakamoto 2001, (B) A. Pott, In: Pott and Pott, 2000).

For the qualitative analysis of A. platensis natural populations, parts of the samples were preserved with 4% formalin and later included in the collection of the State Scientific Herbarium “Maria Eneida P. Kauffman Fidalgo” (SP), in the Institute of Botany, São Paulo, Brazil. For the isolation of strains, the samples were kept under refrigeration in a Styrofoam box with ice, and then transported to the laboratory of the Institute of Botany. The isolation was made using microscope and a micropipette to transfer individuals to tubes with BG-11 (Stanier et al., 1971) liquid media, adjusted to pH 9.5 with NaOH (Santos et al., 2011). The three isolate strains were included in the Institute of Botany Culture Collection (CCIBt), São Paulo, Brazil, and kept under controlled conditions: temperature 23°C, irradiance 40–50 μmol photons m -2 s -1, photoperiod 14-10 h light-dark, and transferred to new flasks every 40 days. In the studied lakes, the parameters temperature, salinity, conductivity and pH were measured with a WTW 340i probe.

 2.2 Morphological analysis

Morphological analysis was performed based on nature material using optical microscope. The following characters were analyzed: 1) general trichome morphology and measurements; 2) apical cells morphology and measurements; 3) cells morphology and measurements; 4) number of spirals per trichome; 5) spirals height; 6) distance between spirals; 7) presence of aerotopes; 8) presence of mucilaginous envelope using China Ink.

 2.3 Molecular analysis 2.3.1 DNA extraction, PCR amplification and sequencing

DNA extraction was performed according to (Fiore et al., 2000). The 16S rDNA and the 16S-23S ITS region amplification was performed using the primers 27F1 (5′-AGA​GTT​TGA​TCC​TGC​TCA​G-3′) (Neilan et al., 1997), and 23S30R (5′-CTT​CGC​CTC​TGT​GTG​CCT​AGG​T-3′) (Lepère et al., 2000) under the following conditions: Heat 94°C/5 min; 10 cycles 94°C/45s, 57°C/45s, 72°C/2min; 25 cycles 94°C/45s, 54°C/45s, 72°C/2min and final extension 72°C/7min, and then sequenced. The inserts were cloned into “pGEM®-T Easy Vector Systems” (Promega, Madison, WI, EUA), pGEM®–T Easy Vector System (Promega, Madison, WI, United States) according to the supplier’s manual, cloned by heat–shock in E. coli DH5α cells and plated for blue–white selection (Sambrook et al., 1989). Three white colonies for each strain were selected and the plasmid extractions were performed by the alkaline lysis method (Birnboim and Doly, 1979). The gene was sequenced using “Big Dye Terminator” version 3.0 (Applied Biosystems) with the plasmid primers T7 and M13 and the internal primers 357F/357R, 704F/704R and 1114F/1114R (Lane, 1991). The sequences were deposited in NCBI under the accession numbers: Arthrospira SM CCIBt3335 (OR142670), Arthrospira IP CCIBt3336 (OR142671) and Arthrospira PSol CCIBt3254 (OR142672).

 2.3.2 Phylogenetic analysis

The 16S rDNA sequences obtained in this study were aligned using ClustalW, in MEGA11: Molecular Evolutionary Genetics Analysis version 11 (Tamura et al., 2021), with Cyanobacteria reference sequences of Microcoleaceae, Oscillatoriaceae and Desertifilaceae, retrieved from GenBank. The final dataset contained 42 sequences with 1,247 informative sites. The phylogenetic trees were built using Maximum Likelihood and Bayesian Inference analysis. GTR + G + I evolutionary model was selected by MEGA 11. The robustness of ML tree was estimated by bootstrap percentages, using 1,000 replications using IQ-Tree online version v1.6.12 (Trifinopoulos et al., 2016). Bayesian trees were constructed in two independent runs, with four chains each, for 5 × 106 generations, burnin fraction set to 0.25, sample frequency 1,000, using MrBayes (Ronquist et al., 2012) in Cipres Gateway (Miller et al., 2010).

 3 Results 3.1 Phyloegenetic analysis

The Bayesian Inference Phylogeny (Figure 2) (average standard deviation of split frequencies: 0.0095), shows strong backbone support and shows our strains L. platensis CCIBt3335, CCIBt3336 and CCIBt3254 strongly supported (BI = 1) in the monophyletic Limnospira clade (Nowicka-Krawczyk et al., 2019), close related to the Microcoleaceae genera Neolyngbya, Lyngbya, Capilliphycus and Limnoraphis (BI = 1), and distant from the Arthrospira clade, represented by the reference strains A. jenneri A10 MDA (Nowicka-Krawczyk et al., 2019). The Maximum Likelihood phylogeny (Figure 3), although presents weak backbone support, corroborates with the BI phylogeny, showing Pantanal strains in the Limnospira clade (boostrap 99), again related to that same Microcoleaceae genera. In both BI and ML trees Arthrospira clade is related to Microcoleus, Tychonema and Okeania.

16S rDNA Bayesian phylogenetic analysis. Posterior probabilities are indicated at nodes.

16S rDNA Maximum Likelihood phylogenetic analysis. Bootstrap support is indicated at nodes.

 3.2 Description of species

Limnospira platensis (Gomont) K. R. S. Santos and G. S. Hentschke comb. nov. (Figures 4–6).

(A–D) General aspect of regularly coiled trichomes with aerotopes, (E) Trichome without aerotopes, (F) Hormogonia with granulose protoplasm and without aerotopes. Scales = 10 µm.

(A–F) Life cycle of L. platensis, (A) Hormogonia in nature (Salina do Meio), (B, C) Hormogonia in culture, (D) Adult trichome in culture, (E) Trichome with necridium (arrow), (F) Trichome broken at necridium, (G, H) Trichome in culture.

(A–D) Morphological variability of L. platensis from Pantanal da Nhecolândia, (A, B) Trichomes with granulose protoplasm, slightly widened and narrowed apexes, (C) Trichome with mucilaginous envelope (rare), (D) Regularly coiled trichome, with aerotopes (Santos and Sant’Anna, 2010), (E) Original illustration of A. platensis (iconotype) showing trichome with rounded terminal cells, without calyptra (Gomont, 1892, pl. 7, fig. 27), (F) Original illustration of A. maxima (iconotype) showing trichome with calyptra (arrows) (Gardner, 1917, pl. 33, Figure 3). Scales = 10 µm.

Basonym: A. platensis Gomont 1892: 247, pl. VII: fig. 27.

Published in: Gomont, M. (1892′1893′). Monographie des Oscillariées (Nostocacées Homocystées). Deuxième partie—Lyngbyées. Annales des Sciences Naturelles, Botanique, Série 7 16: 91-264, pls 1-7.

Trichomes solitary, regularly coiled when adult, constricted or not, 35-151 (−371) μm long, rarely with mucilaginous envelope. Spirals height 16–32.4 μm; distance between spirals 22–40.6 μm. Cells shorter than wide (rarely longer than wide), 1.7–5.3 (−9) μm long and 4–7.8 μm wide. Ratio L/W = 0.5-1.3. Apical cells rounded, slightly narrowed (rarely widened). Cell content blue-green, granulose, with facultative aerotopes.

Habitat: Saline and alkaline lakes, planktonic and more rarely benthic (part of the life cycle).

Reference strain: Arthrospira PSol CCIBt3254 (OR142672)

Studied material: BRAZIL. Mato Grosso do Sul: Corumbá, Pantanal da Nhecolândia, Salina do Meio, 09/25/2005, K.R.S. Santos and C.F.S. Malone (SP390917), 04/22/2006, K.R.S. Santos (SP390919), 08/28/2006, K.R.S. Santos (SP390922), 05/04/2007, K.R.S. Santos (SP390927), 08/19/2009, K.R.S. Santos (SP400654), 10/27/2011, K.R.S. Santos (SP427290), 05/06/2012, C.F.S. Malone and C.L. Sant’Anna (SP427740); Salina Pantanal, 08/16/2009, K.R.S. Santos (SP427747); Salina da Reserva, 08/19/2009, K.R.S. Santos (SP401692), 05/06/2012, C.F.S. Malone and C.L. Sant’Anna (SP427741); Salina da Ponta, 08/19/2009, K.R.S. Santos (SP401691); Salina Pedra do Sol, 08/26/2006, K.R.S. Santos (SP427742), 11/16/2006, K.R.S. Santos (SP427743.

Comments: L. platensis is morphologically different from the other species of the genus, L. fusiformis, L. indica and L. maxima. L. platensis is the only species of the genus without calyptra. Furthermore, L. fusiformis presents irregular spirals, while the spirals of L. platensis are regular. Although the presence of calyptra in L. maxima, this is the most similar species to A. platensis and the differences are discussed below. According to Komárek and Anagnostidis (2005), the relations between L. maxima and L. indica are unclear, and maybe these species are synonyms. When comparing our L. platensis cultures with the nature material, we did not find significative morphological differences between them.

 3.3 Occurrence of <italic>L. platensis</italic> and physicochemical parameters

L. platensis was found forming blooms with A. elenkinii Miller in saline and alkaline lakes in “Pantanal da Nhecolândia”. Table 1 shows all the studied samples and the respective physicochemical parameters. The species occurred in Pantanal saline lakes with pH ranging from 9.2 to 10.2, electrical conductivity from 716 to 19020 μS cm-1 and temperature from 22.8 to 33.3°C. In Lagoa Salitrada Campo Dora the species was registered only once, in the dry season (pH 8.4, electrical conductivity 1852 μS cm-1 and temperature 32°C). These data evidence the dependence of the alkaline pH, high electrical conductivity and warm temperatures for the growing of L. platensis. These conditions also indicate that high nutrient levels are fundamental for the species.

Sampling location, geographical coordinates, Herbarium identification and physicochemical parameters of Pantanal da Nhecolândia lakes.

Lake Coordinates Herbarium ID Sampling date pH Electrical conductivity (µS/cm) Temp. (°C)
Salina do Meio 18°58′29″S, 56°38′47″W SP400654 19/08/2009 9.47 10,600 32.5
Salina do Meio 18°58′29″S, 56°38′47″W SP427290 27/10/2011 9.89 4,060 29.7
Salina do Meio 18°58′29″S, 56°38′47″W SP427740 06/05/2012 9.64 8,770 -
Salina do Meio 18°58′29″S, 56°38′47″W SP390917 25/09/2005 9.85 19,020 23.3
Salina do Meio 18°58′29″S, 56°38′47″W SP390919 22/04/2006 10.16 2,870 33.3
Salina do Meio 18°58′29″S, 56°38′47″W SP390922 28/08/2006 10.09 12,070 24.7
Salina do Meio 18°58′29″S, 56°38′47″W SP390927 04/05/2007 10.19 3,890 31.0
Salina da Reserva 18°57′35″S, 56°37′18″W SP400842 09/05/2005 - 2,380 22.9
Salina da Reserva 18°57′35″S, 56°37′18″W SP401692 19/08/2009 9.68 11,040 34.1
Salina da Reserva 18°57′35″S, 56°37′18″W SP427741 06/05/2012 10.10 5,435 -
Salina da Ponta 18°58′56″S, 56°39′33″W SP400843 25/09/2005 9.9 5,790 23.8
Salina da Ponta 18°58′56″S, 56°39′33″W SP400845 22/04/2006 9.8 864 32.8
Salina da Ponta 18°58′56″S, 56°39′33″W SP400487 28/08/2006 9.8 8,970 22.8
Salina da Ponta 18°58′56″S, 56°39′33″W SP400849 17/11/2006 9.9 716 30.8
Salina da Ponta 18°58′56″S, 56°39′33″W SP401691 19/08/2009 9.22 8,130 30.4
Salina Pedra do Sol 19°10′36″S, 56°57′44″W SP427742 26/08/2006 10.06 2,100 28.0
Salina Pedra do Sol 19°10′36″S, 56°57′44″W SP427743 16/11/2006 10.23 12,200 32.0
Salina Pantanal 18°55′40″S, 56°33′03″W SP427747 16/08/2009 - - -
Salitrada Campo Dora 18°58′05″S, 56°38′58″W SP390925 16/11/2006 8.42 1852 32.0
4 Discussion

Arthrospira and Limnospira are monophyletic genera, morphologically distinguished from each other by the presence of aerotopes in Limnospira (Nowicka-Krawczyk et al., 2019). Aerotopes were commonly observed in our nature samples and isolate strains, and we also observed that this character was facultative in all or our samples and cultures. Apart of the facultative aerotopes, our specimens fit perfectly in the original description of A. platensis (Gomont, 1892). Figure 5 and Table 2 summarize the morphological similarity between our populations and A. platensis, and show the differences between this species and L. maxima, A. platensis presents smaller trichomes and the terminal cells lack calyptra, while L. maxima presents larger trichomes and presents calyptra at the terminal cells.

Morphological characters of “A. platensis” and its most similar species A. maxima according to different authors.

Species Aerotopes/habit Trichome constriction Trichome apex Trichome width (µm) Cell length (µm) Spirals height (µm) Distance between spirals (µm) Location and sampling date Figure
A. platensis (Nordstedt) Gomont (1892) original description ?/? Slightly constrict Slightly or not narrowed. Rounded, without calyptra 6–8 2–6 26–36 43–57 Montevideo, Uruguay, 1884
A. platensis sensu Santos and Sant’Anna (2010) and present study Facultative/planktonic Constrict or not Slightly or not narrowed. Rounded without calyptra 4–7.8 1.7–5.3 (9) 16.0–32.4 22.0–40.6 Saline lakes at Pantanal da Nhecolândia, Brazil; 2004–2012
A. platensis sensu Komárek and Anagnostidis (2005) Absent/periphytic, benthic Slightly constrict Slightly or not narrowed. Rouded with calyptra (4) 6-7 (9?) - (20?) 26-36 (24?) 30-57 South America
A. platensis sensu Komárek and Lund 1990 Absent/benthic Slightly constrict Slightly narrowed (4) 6-7 (8?) - 26–36 30–57 South America
Arthrospira maxima Gardner (1917) Present/planktonic Not constrict Slightly narrowed with calyptra 7–9 5–7 40–60 70–80 Warm saline lakes at South California, Key Route Power-house, Oakland (United States of America) 1916
Arthrospira maxima sensu Komárek and Komárková-Legnerová (2002) Present/planktonic Slightly or not constrict Slightly or not narrowed with calyptra 8-(12) Shorter than wide, rarely isodiametric (34.5)-51 ±(34.5)-51 Vulcanic alkaline lakes at Atlacoya (Tecuitlapa) e El Rincon de Parangueo, México; 1992-1993

?=not reported.

According to the original description, based on an herbarium exsiccate, A. platensis presents cylindrical trichomes, not narrowed toward the ends or slightly narrowed, 6–8 μm wide; spiral height 26–36 μm, distance between spirals 43–57 μm; cells isodiametric 2–6 μm long with granulose protoplasm. The author does not mention the occurrence of aerotopes, nor the habits benthic or planktonic for this species described from La Plata Basin. A brief indication of the species habit is described as “greenish trichomes, forming a thin layered stratum” (free translation from Latin). These morphological and habit descriptions do not exclude the possibility of the presence of aerotopes and the planktonic habit, but make these characters uncertain, considering that the specimens analyzed were from dried material. The expression “thin layered stratum” does not specify where it was sampled and also, at that time, planktonic material was not analyzed, so the author didn´t know if specimens were also growing in phytoplankton. Consequently, we consider the original description poor and encompassing only a little portion of the morphological and ecological plasticity of the species.

Komárek and Lund (1990), also based on the type material in herbarium exsiccate, considered A. platensis as benthic and without aerotopes. However, to consider the species benthic based on the lack of aerotopes in dry preserved specimens is questionable. Currently it is known that aerotopes collapse in dry material (Tomaselli, 1997), and probably that is the reason why these authors (and Gomont) do not mention the presence of these structures.

Considering that, all of our studied populations from nineteen lakes, fit in the original description of A. platensis. All populations presented facultative aerotopes, and because of that, in this paper, we add this character to the species description. This is in agreement with Jeeji-Bai (1999) genus revision, which also report aerotopes for A. platensis. Moreover, the benthic habit was stated by (Komárek and Lund, 1990) as a consequence of the lack of aerotopes, however, in our observations it is clear that the populations are planktonic.

Our ecological data corroborates with the morphological analysis. Although Pantanal and La Plata Basin are geographically distant, they are connected by the rivers Paraguay and Paraná. Pantanal is a corridor connecting the Amazon and La Plata Basins, and during the rainy seasons, the lakes are connected to these basins. The rivers flow to the South carrying a great diversity of species to La Plata estuary, which presents also high salinity, as observed by us in Pantanal lakes (Alho and Gonçalves, 2005; Junk and Cunha, 2005). Another important fact that can explain the presence of A. platensis in La Plata Basin, is that many birds migrate from this area to Pantanal every year for reproduction, and can carry the microbiota in their feathers. It is impossible to know from which environment the original described population was sampled, but we hypothesize that it could be found in temporary small pools (forming stratum), with higher salinity, pH and/or temperature. Clearly, A. platensis is not typical of La Plata Basin, considering that after the description of this species in 1892, even with many sampling efforts of Argentinian and Uruguayan research groups, no populations of A. platensis were found in this region.

The fact that A. platensis was never reported for La Plata Basin after the original description indicates that this species is more related to the salines of Pantanal, due to its abundance and wide distribution in this Biome. According to our results, L. platensis is typical from alkaline, saline, nutrient rich and warm environments and this can explain why the species is rare in La Plata Basin. The species is reported worldwide, but its actual distribution is unknown, mainly because of misidentification of populations in Europe, America, Africa and Asia (Sili et al., 2012).

According to these statements and our phylogenetic analysis showing our strains in Limnospira clade, we conclude that A. platensis has to be combined to L. platensis. Also, we recommend to use Arthrospira PSol CCIBt3254 (OR142672) as the reference strain for the species.

 Data availability statement

The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: GenBank OR142670-OR142672.

 Author contributions

KS (Article initiative, generated morphological data, described the species, collaborated in discussion section) GH (Article initiative, wrote the paper) GF (collaborated in discussion section) AA (generated molecular data) MF (generated molecular data) VV (collaborated in discussion section, revised the manuscript) CS’A (Author of the project, revised the manuscript). All authors contributed to the article and approved the submitted version.

 Funding

GH is funded by Programa de Recuperação e Resiliência Português (PRR), Blue Bioeconomy Innovation Pact, Call 02/C05-I01/2022, Application Number C632741873-00467082. VV thanks to FCT Projects UIDB/04423/2020 and UIDP/04423/2020. KS thanks the financial supports of Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP—Process number 09/51655-1); the Embrapa Pantanal for permission to collect phytoplankton samples in Nhumirim farm, the Dr. Arnaldo Yoso Sakamoto (Universidade Federal de Mato Grosso do Sul—UFMS Campus TrêsLagoas) for the logistical support for the collections expeditions; the Freitag Laboratórios (Timbó/SC, Brazil) for supporting this publication. GF thanks to Laboratorio Tecnologico del Uruguay (LATU) for financial support.

 Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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