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<front>
<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Ecol. Evol.</journal-id>
<journal-title>Frontiers in Ecology and Evolution</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Ecol. Evol.</abbrev-journal-title>
<issn pub-type="epub">2296-701X</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="doi">10.3389/fevo.2017.00060</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Ecology and Evolution</subject>
<subj-group>
<subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Systematic Environmental Impact Assessment for Non-natural Reserve Areas: A Case Study of the Chaishitan Water Conservancy Project on Land Use and Plant Diversity in Yunnan, China</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name><surname>Zhu</surname> <given-names>Zhi-Xin</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/335221/overview"/>
</contrib>
<contrib contrib-type="author">
<name><surname>Zhao</surname> <given-names>Kun-Kun</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/444830/overview"/>
</contrib>
<contrib contrib-type="author">
<name><surname>Lin</surname> <given-names>Qin-Wen</given-names></name>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/422683/overview"/>
</contrib>
<contrib contrib-type="author">
<name><surname>Qureshi</surname> <given-names>Salman</given-names></name>
<xref ref-type="aff" rid="aff3"><sup>3</sup></xref>
<xref ref-type="aff" rid="aff4"><sup>4</sup></xref>
<xref ref-type="aff" rid="aff5"><sup>5</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/119854/overview"/>
</contrib>
<contrib contrib-type="author">
<name><surname>Ross Friedman</surname> <given-names>Cynthia</given-names></name>
<xref ref-type="aff" rid="aff6"><sup>6</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/213071/overview"/>
</contrib>
<contrib contrib-type="author">
<name><surname>Cai</surname> <given-names>Guo-Yin</given-names></name>
<xref ref-type="aff" rid="aff7"><sup>7</sup></xref>
<xref ref-type="aff" rid="aff8"><sup>8</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/442628/overview"/>
</contrib>
<contrib contrib-type="author" corresp="yes">
<name><surname>Wang</surname> <given-names>Hua-Feng</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="author-notes" rid="fn001"><sup>&#x0002A;</sup></xref>
<uri xlink:href="http://loop.frontiersin.org/people/386056/overview"/>
</contrib>
</contrib-group>
<aff id="aff1"><sup>1</sup><institution>Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University</institution> <country>Haikou, China</country></aff>
<aff id="aff2"><sup>2</sup><institution>Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, The Chinese Academy of Sciences</institution> <country>Beijing, China</country></aff>
<aff id="aff3"><sup>3</sup><institution>Department of Geography (Landscape Ecology), Humboldt University of Berlin</institution> <country>Berlin, Germany</country></aff>
<aff id="aff4"><sup>4</sup><institution>Integrative Research Institute on Transformations of Human-Environment Systems, Humboldt University of Berlin</institution> <country>Berlin, Germany</country></aff>
<aff id="aff5"><sup>5</sup><institution>School of Architecture, Birmingham City University</institution> <country>Birmingham, United Kingdom</country></aff>
<aff id="aff6"><sup>6</sup><institution>Department of Biological Sciences, Thompson Rivers University</institution> <country>Kamloops, BC, Canada</country></aff>
<aff id="aff7"><sup>7</sup><institution>Department of Remote Sensing Science and Technology, School of Geomatics and Urban Spatial Informatics, Beijing University of Civil Engineering and Architecture</institution> <country>Beijing, China</country></aff>
<aff id="aff8"><sup>8</sup><institution>Beijing Advanced Innovation Center for Future Urban Design, Beijing University of Civil Engineering and Architecture</institution> <country>Beijing, China</country></aff>
<author-notes>
<fn fn-type="edited-by"><p>Edited by: Luuk Fleskens, Wageningen University and Research, Netherlands</p></fn>
<fn fn-type="edited-by"><p>Reviewed by: Karl Kunert, University of Pretoria, South Africa; Safdar Ali Shirazi, University of the Punjab, Pakistan</p></fn>
<fn fn-type="corresp" id="fn001"><p>&#x0002A;Correspondence: Hua-Feng Wang <email>wanghuafeng2012&#x00040;foxmail.com</email></p></fn>
<fn fn-type="other" id="fn002"><p>This article was submitted to Agroecology and Land Use Systems, a section of the journal Frontiers in Ecology and Evolution</p></fn></author-notes>
<pub-date pub-type="epub">
<day>23</day>
<month>06</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="collection">
<year>2017</year>
</pub-date>
<volume>5</volume>
<elocation-id>60</elocation-id>
<history>
<date date-type="received">
<day>19</day>
<month>10</month>
<year>2016</year>
</date>
<date date-type="accepted">
<day>24</day>
<month>05</month>
<year>2017</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#x000A9; 2017 Zhu, Zhao, Lin, Qureshi, Ross Friedman, Cai and Wang.</copyright-statement>
<copyright-year>2017</copyright-year>
<copyright-holder>Zhu, Zhao, Lin, Qureshi, Ross Friedman, Cai and Wang</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/"><p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p></license>
</permissions>
<abstract>
<p>Environmental impact assessment (EIA) before and after the establishment of a Water Conservancy Project (WCP) is of great theoretical and practical importance for assessing the effectiveness of ecological restoration efforts. WCPs rehabilitate flood-damaged areas or other regions hit by disasters by controlling and redistributing surface water and groundwater. Using Geographic Information System (GIS) and Composite Evaluation Index (CEI) in predictive modeling, we studied the degree to which a WCP could change land use, plant communities, and species diversity in Yunnan, China. Via modeling, we quantified likely landscape pattern changes and linked them to naturality (i.e., the percentage of secondary vegetation types), diversity, and stability together with the human interferences (e.g., conservation or restoration project) of an ecosystem. The value of each index was determined by the evaluation system, and the weight percentage was decided through Analytical Hierarchy Process (AHP). We found that minor land-use changes would occur after the Chaishitan WCP was theoretically established. The greatest decline was farmland (0.079%), followed by forest (0.066%), with the least decline in water bodies (0.020%). We found 1,076 vascular plant species (including subspecies, varieties and form) belonging to 165 families and 647 genera in Chaishitan irrigation area before the water conservancy establishment. The naturality and diversity decreased 11.18 and 10.16% respectively. The CEI was 0.92, which indicated that Chaishitan WCP will enhance local landscape heterogeneity, and it will not deteriorate local ecological quality. Our study proposes a comprehensive ecological evaluation system for this WCP and further suggests the importance of including the ecological and environmental consequences of the WCP, along with the well-established socioeconomic evaluation systems for non-natural reserve areas. We conclude that the Chaishitan WCP will have minor environmental impacts on the local landscape and plant diversity. Furthermore, the irrigation project will provide sufficient water once established, which will enrich local plant diversity; therefore, we support its construction.</p>
</abstract>
<kwd-group>
<kwd>modeling</kwd>
<kwd>water conservancy project</kwd>
<kwd>landscape index</kwd>
<kwd>plant diversity</kwd>
<kwd>ecological evaluation</kwd>
</kwd-group>
<contract-sponsor id="cn001">Ministry of Science and Technology of the People&#x00027;s Republic of China<named-content content-type="fundref-id">10.13039/501100002855</named-content></contract-sponsor>
<counts>
<fig-count count="4"/>
<table-count count="7"/>
<equation-count count="4"/>
<ref-count count="66"/>
<page-count count="14"/>
<word-count count="9051"/>
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</article-meta>
</front>
<body>
<sec sec-type="intro" id="s1">
<title>Introduction</title>
<p>Paradigm shifts in water conservation projects toward harmonizing the needs for humans and nature are essential (Liu et al., <xref ref-type="bibr" rid="B26">2013</xref>). Researchers have been paying increasing attention to the socioeconomic impacts of water conservancy projects (WCPs), but their ecological and environmental consequences have received considerably less attention from the scientific community. WCPs rehabilitate flood-damaged areas or other regions hit by disasters by controlling and redistributing surface water and groundwater. In China, the Before-Project environmental impact assessment (EIA) system for major construction projects has been in place for more than 20 years (Chen et al., <xref ref-type="bibr" rid="B9">2013</xref>). There is a standardized evaluation procedure for assessing the potential ecological effects of construction projects in operation, which involves conducting an on-site investigation and evaluation, making environmental impact predictions, and providing an EIA report. However, there is a general lag between the project&#x00027;s implementation and the assessment of its effects, because some construction projects are in the preliminary design phase, others are under construction, while still others have been completed, but they all need an EIA according to the current environmental management requirements. In other words, real-time ecological environment monitoring and evaluation research is scarce after the project&#x00027;s implementation, preventing assessment of the immediate consequences (Lu et al., <xref ref-type="bibr" rid="B28">2003</xref>; Du and Wang, <xref ref-type="bibr" rid="B14">2005</xref>; Chang et al., <xref ref-type="bibr" rid="B8">2006</xref>; Zhang and Hu, <xref ref-type="bibr" rid="B63">2010</xref>). In particular, quantitative assessments, whereby weights are given to the EIA quality indices according to their properties and importance, are lacking (Wang et al., <xref ref-type="bibr" rid="B50">2003</xref>; Sun and Dong, <xref ref-type="bibr" rid="B42">2004</xref>). Neglecting these ecological and environmental impacts may sometimes lead to unintended consequences (e.g., increasing the incidence of chronic diseases worldwide) for ecosystems as well as to declines in the critical ecosystem services provided to our society (Chen et al., <xref ref-type="bibr" rid="B9">2013</xref>). Therefore, conducting field-based assessment for WCPs&#x00027; ecological effects will facilitate WCP construction from an ecological perspective, and thus promote their sustainable development (Dong, <xref ref-type="bibr" rid="B13">2003</xref>; Jiang, <xref ref-type="bibr" rid="B22">2005</xref>). Furthermore, such research is of practical significance for EIAs of ecological restoration projects and of similar projects in future.</p>
<p>Previous studies have focused on the environmental and ecological impacts of specific projects; e.g., impacts of the Three Gorges Hydroelectric Project (TGHP) on ecological processes and biodiversity (Wu et al., <xref ref-type="bibr" rid="B57">2003</xref>, <xref ref-type="bibr" rid="B56">2004</xref>; Xie et al., <xref ref-type="bibr" rid="B60">2003</xref>; Lopez-Pujol and Ren, <xref ref-type="bibr" rid="B27">2009</xref>), environmental and ecological effects of the South-to-North Water Transfer Project (SNWTP) (Zhang, <xref ref-type="bibr" rid="B64">2009</xref>). However, there are three shortcomings in the most recent EIA reports. Firstly, very little work has been conducted for non-rare, non-protected species or non-natural reserve areas, whereas biodiversity refers to a collection of all species. The loss of other common or unprotected species will cause declines in overall biodiversity (Chen et al., <xref ref-type="bibr" rid="B9">2013</xref>). Secondly, there is a lack of an integrated vulnerability index based on independent landscape metrics and anthropic impacts. This kind of integrated index would be helpful for the planning of conservation and protection measures in protected areas (&#x0010C;ern&#x000FD; et al., <xref ref-type="bibr" rid="B7">2013</xref>; Nzeadibe et al., <xref ref-type="bibr" rid="B31">2015</xref>; Caniani et al., <xref ref-type="bibr" rid="B5">2016</xref>). Thirdly, while companies provide reliable quantitative information about their performance (Hammond and Miles, <xref ref-type="bibr" rid="B19">2004</xref>), the industry has information gaps, as it fails to publicly communicate its level of environmental performance (Panwar et al., <xref ref-type="bibr" rid="B34">2014</xref>; M&#x000E4;kel&#x000E4;, <xref ref-type="bibr" rid="B29">2017</xref>).</p>
<p>Furthermore, other studies, such as that by Steffen and Leuschner (<xref ref-type="bibr" rid="B41">2014</xref>) suggested that decade-long human impact on river hydraulics and chemistry can significantly reduce the community diversity at the landscape level, profoundly altering the relative abundance of the assemblages. Chen et al. (<xref ref-type="bibr" rid="B9">2013</xref>) also called for biological diversity impact assessments to be included in any EIA, generating a sound ecological protection and EIA system for established WCPs. Other researchers (e.g., Westman, <xref ref-type="bibr" rid="B53">1985</xref>; Brismar, <xref ref-type="bibr" rid="B3">2004</xref>) also suggested incorporating biological diversity effects into scope of the evaluation, i.e., to investigate the species abundance, distribution, endangered status, and existing problems resulting from the original protection measures. Determining whether it is necessary to protect biological diversity and take corresponding measures depends on local basic biological diversity characteristics and social economic status, which will enable evaluation of WCP scientifically and objectively.</p>
<p>The Chaishitan reservoir, a non-natural reserve area, is located at the interface of Yiliang and Shilin counties in Yunnan province, China (Figure <xref ref-type="fig" rid="F1">1</xref>). Recently, a water conservancy project has been planned for Chaishitan area to enable irrigation; i.e., Computer Aid Design (CAD) digital maps with the main and branch channels have been generated for implementation in 2016. Assessing land cover and plant diversity before project establishment will be invaluable because it would promote plants to flourish due to an increased water supply; such information would provide a baseline that would otherwise be absent once the water conservancy project became established. Therefore, in this study, we hypothesized that the local plant diversity will increase and that the land use will become more fragmented after the water conservancy project is established.</p>
<fig id="F1" position="float">
<label>Figure 1</label>
<caption><p>The plant community sampling sites in Chaishitan water conservancy of Yunnan, China.</p></caption>
<graphic xlink:href="fevo-05-00060-g0001.tif"/>
</fig>
<p>In this study, we conducted a comprehensive land use analysis and performed plant diversity sampling along the channel and the potential impact areas in Yunnan, China. We then investigated the land use and plant species after modeling the establishment of the Chaishitan WCP through a CAD blueprint of the Chaishitan irrigation region. By comparing the landscape pattern index and plant species composition and relative abundance in the regions, we aimed to address the following questions: (1) How could land-use and plant diversity change immediately after the putative completion of the WCP? (2) Whether or not the Chaishitan WCP should be established in Yunnan as evaluated by our &#x0201C;before-and-after&#x0201D; systematic environment impact assessment; i.e., assessing the plant diversity and land use twice before and then after the project established.</p>
</sec>
<sec sec-type="methods" id="s2">
<title>Methods</title>
<sec>
<title>Study area</title>
<p>The Chaishitan reservoir is one of the centerpieces of the Nanpanjiang River and is located upstream of the Zhujiang River (i.e., the Pearl River). The actual maximum water level of the Chaishitan reservoir is 1,643.74 m, and its corresponding capacity is of 0.381 billion m<sup>3</sup>. However, Yiliang city and its surroundings often suffer severe drought. For example, in 2010, the drought areas of Yiliang county totaled 196.2 km<sup>2</sup>, and 104 km<sup>2</sup> of croplands produced nothing due to drought at the end of that year (Anonymous, <xref ref-type="bibr" rid="B1">2011</xref>). In addition, more than 100,000 people had difficulties in accessing safe drinking water because the vast majority of the surface rivers had dried up and the groundwater level had severely decreased in 2010. Therefore, it is urgent to establish an irrigation project (i.e., Chaishitan WCP) from the Chaishitan reservoir to meet the needs of surrounding Yiliang and Shilin counties.</p>
</sec>
<sec>
<title>Land use and remote sensing interpretation</title>
<p>Based on the Current Land Use Classification (GB/T 21010-2007) (CMDLRDC (Cadastral Management Division of Land and Resources Department of China) and LSPI (Lands Surveying and Planning Institute), <xref ref-type="bibr" rid="B10">2007</xref>) issued by China&#x00027;s Land and Resource Ministry, we adopted Current Land Use Classification (Table <xref ref-type="table" rid="T1">1</xref>). As the national standard, China&#x00027;s Land Use Status Classification is mandatory, principled and guiding; therefore, we adopted this unified standard in order to improve the level of land management and promote its application in Yiliang county of Yunnan province. Secondly, China&#x00027;s Land Use Status Classification conforms to China&#x00027;s national conditions; namely, it meets the relevant laws and regulations of China&#x00027;s domestic standards, which makes it easier for the construction project to obtain approval. Thirdly, we adopted the nationwide uniform standards, which facilitate the smooth development of the project&#x00027;s construction work; it will be easier to compare our results with similar projects in China and to provide reference for future related projects (Chen et al., <xref ref-type="bibr" rid="B9">2013</xref>).</p>
<table-wrap position="float" id="T1">
<label>Table 1</label>
<caption><p>The land use area before and after Chaishitan water conservancy project establishment.</p></caption>
<table frame="hsides" rules="groups">
<thead><tr>
<th valign="top" align="left"><bold>First land use types</bold></th>
<th valign="top" align="left"><bold>Secondary land use types</bold></th>
<th valign="top" align="center"><bold>Areas before project established (km<sup>2</sup>)</bold></th>
<th valign="top" align="center"><bold>Areas after project established (km<sup>2</sup>)</bold></th>
<th valign="top" align="center"><bold>Changed areas (km<sup>2</sup>)</bold></th>
<th valign="top" align="center"><bold>Percentage (%)</bold></th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">Urban and industrial land</td>
<td valign="top" align="left">Urban and industrial land</td>
<td valign="top" align="center">37.647</td>
<td valign="top" align="center">37.631</td>
<td valign="top" align="center">&#x02212;0.016</td>
<td valign="top" align="center">&#x02212;0.042</td>
</tr>
<tr>
<td valign="top" align="left">Farmland</td>
<td valign="top" align="left">Farmland</td>
<td valign="top" align="center">204.92</td>
<td valign="top" align="center">204.759</td>
<td valign="top" align="center">&#x02212;0.161</td>
<td valign="top" align="center">&#x02212;0.079</td>
</tr>
<tr>
<td valign="top" align="left">Orchard</td>
<td valign="top" align="left">Orchard</td>
<td valign="top" align="center">55.099</td>
<td valign="top" align="center">55.065</td>
<td valign="top" align="center">&#x02212;0.033</td>
<td valign="top" align="center">&#x02212;0.061</td>
</tr>
<tr>
<td valign="top" align="left">Forest land</td>
<td valign="top" align="left">Other kinds of forest land</td>
<td valign="top" align="center">16.924</td>
<td valign="top" align="center">16.91</td>
<td valign="top" align="center">&#x02212;0.014</td>
<td valign="top" align="center">&#x02212;0.085</td>
</tr>
<tr>
<td/>
<td valign="top" align="left">Forest land</td>
<td valign="top" align="center">156.756</td>
<td valign="top" align="center">156.651</td>
<td valign="top" align="center">&#x02212;0.104</td>
<td valign="top" align="center">&#x02212;0.066</td>
</tr>
<tr>
<td valign="top" align="left">Waters and water conservancy facilities Land</td>
<td valign="top" align="left">Water</td>
<td valign="top" align="center">15.64</td>
<td valign="top" align="center">15.637</td>
<td valign="top" align="center">&#x02212;0.003</td>
<td valign="top" align="center">&#x02212;0.02</td>
</tr>
<tr>
<td/>
<td valign="top" align="left">Water conservancy facilities Land</td>
<td/>
<td valign="top" align="center">0.332</td>
<td valign="top" align="center">0.332</td>
<td/>
</tr>
</tbody>
</table>
</table-wrap>
<p>The methods of remote sensing image interpretation are widely used and have been applied in previous studies (e.g., Qiu and Wang, <xref ref-type="bibr" rid="B36">2010</xref>; Wang et al., <xref ref-type="bibr" rid="B48">2013</xref>, <xref ref-type="bibr" rid="B49">2016</xref>; Booth et al., <xref ref-type="bibr" rid="B2">2016</xref>). The first-level classification was based on land use and land cover (LULC) characteristics, and the secondary classification is primarily based on the first survey characteristics of land management. One scene of Operational Land Imager (OLI) data from Landsat 8 was obtained in March 9, 2015 as the main source for the second survey of LULC classification. The spatial resolution of panchromatic and multispectral OLI was 15 and 30 m, respectively. Three main steps were taken for the classification. First, we performed a multi-resolution image segmentation to generate image objects to which the classification algorithm could be applied. For this segmentation, we used a &#x0201C;scale parameter&#x0201D; of 20, which was determined by visual interpretation of the segmentation results. The segmentation process identified objects that were homogeneous and included the features (i.e., spectral values, shape, texture, etc.) that can be used for classification (Walker and Blaschke, <xref ref-type="bibr" rid="B45">2008</xref>; Qiu and Wang, <xref ref-type="bibr" rid="B36">2010</xref>). Second, once the segmentation was achieved, we utilized a combination of fuzzy rules and a standard nearest neighbor (SNN) algorithm to classify each image object. A total of 200 training samples were selected and referenced with the auxiliary data (including digital topographic maps). We printed 20 images wherein land use or vegetation types could not be determined from the data alone, brought these images into the field, and either found the specific positions or asked the local people to identify the location. In total, 20 field surveys for the unidentified land use or vegetation types were conducted. Google Earth and local specific maps were also referenced in order to identify their representative classes. Based on the spectral and spatial information of these samples, we created the SNN feature space and fuzzy rule algorithms that were collectively used for classification. Each image object was assigned a probability of belonging to each LULC class, and the final class of image objects was decided on the basis of which assigned class has the highest probability. Finally, we refined the classification with manual adjustment to improve the overall quality of classification. The classification was performed using Definiens 7.0 software. Classification accuracy was assessed by comparing the reference collection with classified imagery (Congalton, <xref ref-type="bibr" rid="B11">1991</xref>). Based on the derived LULC map, we further calculated the percentage for each LULC type in ArcGIS 9.3 (ESRI).</p>
<p>We overlaid the proposed CAD digital maps onto the above remote sensing image from Landsat 8, and then we interpreted the image again and predicted LULC changes immediately after the WCP has been built in 2016. Other ancillary data included 1:50,000 topographic maps, 1:250,000 land-use maps from 2014, district administrative maps and related land resources survey data, inventory reports and related maps.</p>
</sec>
<sec>
<title>Plant diversity sampling</title>
<p>The field survey on plant species structure and diversity adopted a previously used sampling protocol (Wang et al., <xref ref-type="bibr" rid="B47">2011</xref>) to investigate the community composition and structure of typical vegetation in the region. The proposed layout of the Chaishitan WCP has one main-channel and two branch channels in Yiliang county, and one major main-channel and five branch channels in Shilin county. According to local people who are familiar with the proposed layout of Channels, we found the specific positions of the future channels passing away, and we set 21 plant diversity sampling plots along the channels or near the channels (Figure <xref ref-type="fig" rid="F1">1</xref>). Once the water conservancy established, most plant species will disappear because of habit loss, therefore, we get the plant diversity before the water conservancy established and predict how could plant diversity change based on the CAD maps and modeling (i.e., we assumed the plant species in or nearby proposed channels will disappear once the water conservancy established in 2016).</p>
<p>We performed plant diversity field investigations twice; the first time was conducted from May to June in 2015 for 27 days with seven people involved in the field work, and the second time was conducted from August to September in 2015 for 42 days with 11 people involved in the field work. We conducted field work according to different land use types: i.e., Secondary needle and broadleaf mixed forest (SF); Wasteland (Wetland) (WL); Eucalyptus plantation (EP); Orchard (OR), and Farmland (FL) (Table <xref ref-type="table" rid="T2">2</xref>). The number of sampling sites with each kind of land use type varied from three to eight; eight sampling sites fell into Farmland. In total, 21 sampling sites were investigated (Table <xref ref-type="table" rid="T2">2</xref>, Supplementary Material <xref ref-type="supplementary-material" rid="SM1">A</xref>). The plant diversity was investigated at three different layers; i.e., tree, shrub, and herb layers. At each sampling site, we investigated one 20 &#x000D7; 20 m tree plot: five 2 &#x000D7; 2 m shrub plots and five 1 &#x000D7; 1 m herb plots were surveyed within the tree plot at its four corners and center. In total, we had 21 tree plots, 105 shrub plots, and 105 herb plots. We recorded each species name, diameter at Breast Height (DBH) for trees, height, crown width, coverage for herb, and origin.</p>
<table-wrap position="float" id="T2">
<label>Table 2</label>
<caption><p>Plant community sampling sites in Chaishitan irrigation region, Yunnan province of China.</p></caption>
<table frame="hsides" rules="groups">
<thead><tr>
<th valign="top" align="left"><bold>Code</bold></th>
<th valign="top" align="left"><bold>Site</bold></th>
<th valign="top" align="left"><bold>Land use types</bold></th>
<th valign="top" align="center"><bold>Longitude</bold></th>
<th valign="top" align="center"><bold>Latitude</bold></th>
<th valign="top" align="center"><bold>Altitude (m)</bold></th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">1</td>
<td valign="top" align="left">Chaishitan Reservoir</td>
<td valign="top" align="left">Secondary needle and broadleaf mixed forest (SF)</td>
<td valign="top" align="center">103.34</td>
<td valign="top" align="center">24.99</td>
<td valign="top" align="center">1,721</td>
</tr>
<tr>
<td valign="top" align="left">2</td>
<td valign="top" align="left">1th tunnel outlet aqueduct</td>
<td valign="top" align="left">Farmland (corn) (FL)</td>
<td valign="top" align="center">103.32</td>
<td valign="top" align="center">25</td>
<td valign="top" align="center">1,640</td>
</tr>
<tr>
<td valign="top" align="left">3</td>
<td valign="top" align="left">East Canal Road</td>
<td valign="top" align="left">Orchard (chestnut) (OR)</td>
<td valign="top" align="center">103.27</td>
<td valign="top" align="center">24.99</td>
<td valign="top" align="center">1,681</td>
</tr>
<tr>
<td valign="top" align="left">4</td>
<td valign="top" align="left">Nuomizhuang village tube</td>
<td valign="top" align="left">Eucalyptus plantation (EP)</td>
<td valign="top" align="center">103.26</td>
<td valign="top" align="center">24.99</td>
<td valign="top" align="center">1,606</td>
</tr>
<tr>
<td valign="top" align="left">5</td>
<td valign="top" align="left">Xinjie</td>
<td valign="top" align="left">Eucalyptus plantation (EP)</td>
<td valign="top" align="center">103.26</td>
<td valign="top" align="center">24.01</td>
<td valign="top" align="center">1,616</td>
</tr>
<tr>
<td valign="top" align="left">6</td>
<td valign="top" align="left">Gengjiaying Canal inverted siphon</td>
<td valign="top" align="left">Farmland (rice) (FL)</td>
<td valign="top" align="center">103.23</td>
<td valign="top" align="center">24.04</td>
<td valign="top" align="center">1,589</td>
</tr>
<tr>
<td valign="top" align="left">7</td>
<td valign="top" align="left">The intersection of Jialonghe and road</td>
<td valign="top" align="left">Wasteland (Wetland) (WL)</td>
<td valign="top" align="center">103.23</td>
<td valign="top" align="center">24.04</td>
<td valign="top" align="center">1,563</td>
</tr>
<tr>
<td valign="top" align="left">8</td>
<td valign="top" align="left">Dacun village</td>
<td valign="top" align="left">Orchard (OR)</td>
<td valign="top" align="center">103.14</td>
<td valign="top" align="center">24.97</td>
<td valign="top" align="center">1,585</td>
</tr>
<tr>
<td valign="top" align="left">9</td>
<td valign="top" align="left">Jiangtoucun village</td>
<td valign="top" align="left">Wasteland (WL)</td>
<td valign="top" align="center">103.12</td>
<td valign="top" align="center">24.93</td>
<td valign="top" align="center">1,556</td>
</tr>
<tr>
<td valign="top" align="left">10</td>
<td valign="top" align="left">Relongtan pond</td>
<td valign="top" align="left">Eucalyptus plantation (EP)</td>
<td valign="top" align="center">103.19</td>
<td valign="top" align="center">24.92</td>
<td valign="top" align="center">1,594</td>
</tr>
<tr>
<td valign="top" align="left">11</td>
<td valign="top" align="left">Head of xihe river</td>
<td valign="top" align="left">Wasteland (WL)</td>
<td valign="top" align="center">103.19</td>
<td valign="top" align="center">24.98</td>
<td valign="top" align="center">1,563</td>
</tr>
<tr>
<td valign="top" align="left">12</td>
<td valign="top" align="left">Baiyihe village</td>
<td valign="top" align="left">Farmland (corn) (FL)</td>
<td valign="top" align="center">103.24</td>
<td valign="top" align="center">24.96</td>
<td valign="top" align="center">1,628</td>
</tr>
<tr>
<td valign="top" align="left">13</td>
<td valign="top" align="left">Xiaoshanhou village</td>
<td valign="top" align="left">Farmland (corn) (FL)</td>
<td valign="top" align="center">103.24</td>
<td valign="top" align="center">24.96</td>
<td valign="top" align="center">1,618</td>
</tr>
<tr>
<td valign="top" align="left">14</td>
<td valign="top" align="left">Xiahousuo village</td>
<td valign="top" align="left">Farmland (corn) (FL)</td>
<td valign="top" align="center">103.25</td>
<td valign="top" align="center">24.98</td>
<td valign="top" align="center">1,592</td>
</tr>
<tr>
<td valign="top" align="left">15</td>
<td valign="top" align="left">Shilin getting water point</td>
<td valign="top" align="left">Secondary needle and broadleaf mixed forest (SF)</td>
<td valign="top" align="center">103.39</td>
<td valign="top" align="center">25</td>
<td valign="top" align="center">1,840</td>
</tr>
<tr>
<td valign="top" align="left">16</td>
<td valign="top" align="left">Linkoupu pond</td>
<td valign="top" align="left">Orchard (apricot) (OR)</td>
<td valign="top" align="center">103.41</td>
<td valign="top" align="center">24.93</td>
<td valign="top" align="center">1,953</td>
</tr>
<tr>
<td valign="top" align="left">17</td>
<td valign="top" align="left">Tianshengguan pond</td>
<td valign="top" align="left">Farmland (tobacco) (FL)</td>
<td valign="top" align="center">103.41</td>
<td valign="top" align="center">24.91</td>
<td valign="top" align="center">1,923</td>
</tr>
<tr>
<td valign="top" align="left">18</td>
<td valign="top" align="left">New floodgate of Tianshengguan</td>
<td valign="top" align="left">Secondary needle and broadleaf mixed forest (SF)</td>
<td valign="top" align="center">103.42</td>
<td valign="top" align="center">24.89</td>
<td valign="top" align="center">1,927</td>
</tr>
<tr>
<td valign="top" align="left">19</td>
<td valign="top" align="left">Shilaohu mountain</td>
<td valign="top" align="left">Farmland (tobacco) (FL)</td>
<td valign="top" align="center">103.4</td>
<td valign="top" align="center">24.96</td>
<td valign="top" align="center">1,982</td>
</tr>
<tr>
<td valign="top" align="left">20</td>
<td valign="top" align="left">Tuanjiehu lake</td>
<td valign="top" align="left">Secondary needle and broadleaf mixed forest (SF)</td>
<td valign="top" align="center">103.35</td>
<td valign="top" align="center">24.9</td>
<td valign="top" align="center">1,864</td>
</tr>
<tr>
<td valign="top" align="left">21</td>
<td valign="top" align="left">Bajiang river</td>
<td valign="top" align="left">Farmland (corn) (FL)</td>
<td valign="top" align="center">103.34</td>
<td valign="top" align="center">24.85</td>
<td valign="top" align="center">1,814</td>
</tr>
</tbody>
</table>
</table-wrap>
</sec>
<sec>
<title>Data analysis</title>
<sec>
<title>Landscape analysis</title>
<p>Vector graphics were converted into landscape classification grid maps using ArcInfo, and landscape indices were calculated with the Fragstats3.3 (McGarigal et al., <xref ref-type="bibr" rid="B30">2015</xref>) at the landscape level. Patch density (PD), fractal dimension (FD), the clustered index (CONT), dominance index (LDI), degrees of separation index (SPLI), and Shannon diversity indices (SHDI), Number of Patches (NP), Edge Density (ED), Largest Patch Index (LPI), Percentage of Landscape (PLAND), Landscape Shape Index (LSI), Shape Index (Mean) (SHAPE_MN), and Fractal Dimension Index (FRAC_AM) were selected to quantify the modeled and predicted landscape pattern changes (see the concept of each index in Table <xref ref-type="table" rid="T3">3</xref>). These landscape pattern indices were then linked to &#x0201C;naturality&#x0201D; (i.e., the percentage of secondary vegetation types), diversity, stability, and human interference in order to establish an evaluation system, which could then be used to estimate the ecological condition of the Chaishitan WCP immediately after its establishment in 2016.</p>
<table-wrap position="float" id="T3">
<label>Table 3</label>
<caption><p>Ecological assessment index used in this study.</p></caption>
<table frame="hsides" rules="groups">
<thead><tr>
<th valign="top" align="left"><bold>Guide line layer</bold></th>
<th valign="top" align="left"><bold>Target layer</bold></th>
<th valign="top" align="left"><bold>Concept of index</bold></th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">Naturality</td>
<td valign="top" align="left">Percentage of vegetation type (Secondary needle- and broad-leaves mixed forest) (PV)</td>
<td valign="top" align="left">Percentage of vegetation type area and total area</td>
</tr>
<tr>
<td valign="top" align="left">Diversity</td>
<td valign="top" align="left">Shannon diversity index (SHDI)</td>
<td valign="top" align="left">Diversity index reflects the number of landscape type and its percentage</td>
</tr>
<tr>
<td valign="top" align="left">Stability</td>
<td valign="top" align="left">Landscape dominance Index (LDI)</td>
<td valign="top" align="left">LDI reflects the degree of little patch dominant in landscape</td>
</tr>
<tr>
<td/>
<td valign="top" align="left">Contagion Index (CONTAG)</td>
<td valign="top" align="left">CONTAG reflects spatial distribution of landscape type</td>
</tr>
<tr>
<td/>
<td valign="top" align="left">Landscape splitting Index (SPLIT)</td>
<td valign="top" align="left">Bigger splitting index reflects dispersed landscape type and worse stability</td>
</tr>
<tr>
<td valign="top" align="left">Threat of human interference</td>
<td valign="top" align="left">Human disturbance (HD)</td>
<td valign="top" align="left">Percentage of human interferential and natural landscape</td>
</tr>
<tr>
<td/>
<td valign="top" align="left">Perimeter-Area Fractal Dimension (PAFRAC)</td>
<td valign="top" align="left">PAFRAC reflects complexity of landscape shape, the value is between 1 and 2 (Qiu et al., <xref ref-type="bibr" rid="B37">2007</xref>)</td>
</tr>
<tr>
<td/>
<td valign="top" align="left">Patch density (PD)</td>
<td valign="top" align="left">The number of patches of the corresponding patch type divided by total landscape area (m<sup>2</sup>)</td>
</tr>
</tbody>
</table>
</table-wrap>
<p>In light of current ecological assessment systems (O&#x00027;Neill et al., <xref ref-type="bibr" rid="B33">1988</xref>; Zheng et al., <xref ref-type="bibr" rid="B65">1994</xref>; Xia et al., <xref ref-type="bibr" rid="B59">2005</xref>; Guo et al., <xref ref-type="bibr" rid="B17">2007</xref>), naturality, diversity, stability, and threat of human interference were selected as assessment indicators (Table <xref ref-type="table" rid="T3">3</xref>). The weight of each evaluation index was determined by an analytic hierarchy process (Yang and Xiao, <xref ref-type="bibr" rid="B62">2000</xref>; Xu et al., <xref ref-type="bibr" rid="B61">2002</xref>; Guo and Wang, <xref ref-type="bibr" rid="B18">2005</xref>; Wan et al., <xref ref-type="bibr" rid="B46">2005</xref>). Ecological assessment indicators with three levels were selected and evaluated from the perspective of ecological protection (Tables <xref ref-type="table" rid="T4A">4A</xref>,<xref ref-type="table" rid="T4B">B</xref>).</p>
<table-wrap position="float" id="T4A">
<label>Table 4A</label>
<caption><p>The landscape index and Composite evaluation index before Chaishitan water conservancy project establishment.</p></caption>
<table frame="hsides" rules="groups">
<thead><tr>
<th valign="top" align="left"><bold>Guide line layer</bold></th>
<th valign="top" align="center"><bold>Naturality</bold></th>
<th valign="top" align="center"><bold>Diversity</bold></th>
<th valign="top" align="center" colspan="3"><bold>Threat of human interference</bold></th>
<th valign="top" align="center" colspan="3"><bold>Stability</bold></th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">Weight</td>
<td valign="top" align="center">0.21</td>
<td valign="top" align="center">0.15</td>
<td valign="top" align="center" colspan="3">0.41</td>
<td valign="top" align="center" colspan="3">0.24</td>
</tr>
<tr style="border-top: thin solid #000000;">
<td valign="top" align="left"><bold>Index</bold></td>
<td valign="top" align="center"><bold>Percentage of vegetation type (PV)</bold></td>
<td valign="top" align="center"><bold>Shannon diversity index (SHDI)</bold></td>
<td valign="top" align="center"><bold>Human disturbance (HD)</bold></td>
<td valign="top" align="center"><bold>Patch density (PD)</bold></td>
<td valign="top" align="center"><bold>Perimeter-Area Fractal Dimension (PAFRAC)</bold></td>
<td valign="top" align="center"><bold>Contagion Index (CONTAG)</bold></td>
<td valign="top" align="center"><bold>Landscape dominance Index (LDI)</bold></td>
<td valign="top" align="center"><bold>Landscape splitting Index (SPLIT)</bold></td>
</tr>
<tr style="border-top: thin solid #000000;">
<td valign="top" align="left">Value</td>
<td valign="top" align="center">21.60</td>
<td valign="top" align="center">1.36</td>
<td valign="top" align="center">0.51</td>
<td valign="top" align="center">9.25</td>
<td valign="top" align="center">1.21</td>
<td valign="top" align="center">59.87</td>
<td valign="top" align="center">0.87</td>
<td valign="top" align="center">7.95</td>
</tr>
<tr>
<td valign="top" align="left">Standardization of value</td>
<td valign="top" align="center">1.76</td>
<td valign="top" align="center">0.07</td>
<td valign="top" align="center">0.00</td>
<td valign="top" align="center">0.73</td>
<td valign="top" align="center">0.06</td>
<td valign="top" align="center">4.96</td>
<td valign="top" align="center">0.03</td>
<td valign="top" align="center">0.62</td>
</tr>
<tr>
<td/>
<td/>
<td/>
<td valign="top" align="center" colspan="3">0.26</td>
<td valign="top" align="center" colspan="3">1.85</td>
</tr>
<tr style="border-top: thin solid #000000;">
<td valign="top" align="left"><bold>Guide line layer</bold></td>
<td valign="top" align="center"><bold>Naturality</bold></td>
<td valign="top" align="center"><bold>Diversity</bold></td>
<td valign="top" align="center"><bold>Threat of human interference</bold></td>
<td valign="top" align="center"><bold>Stability</bold></td>
<td/>
<td/>
<td/>
<td/>
</tr>
<tr style="border-top: thin solid #000000;">
<td valign="top" align="left">Index value</td>
<td valign="top" align="center">1.76</td>
<td valign="top" align="center">0.07</td>
<td valign="top" align="center">0.26</td>
<td valign="top" align="center">1.85</td>
<td/>
<td/>
<td/>
<td/>
</tr>
<tr>
<td valign="top" align="left">Weight</td>
<td valign="top" align="center">0.21</td>
<td valign="top" align="center">0.15</td>
<td valign="top" align="center">0.41</td>
<td valign="top" align="center">0.24</td>
<td/>
<td/>
<td/>
<td/>
</tr>
<tr>
<td valign="top" align="left">Composite evaluation index (CEI)</td>
<td valign="top" align="center">0.92</td>
<td/>
<td/>
<td/>
<td/>
<td/>
<td/>
<td/>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap position="float" id="T4B">
<label>Table 4B</label>
<caption><p>The landscape index and Composite evaluation index after Chaishitan water conservancy project establishment.</p></caption>
<table frame="hsides" rules="groups">
<thead><tr>
<th valign="top" align="left"><bold>Guide line layer</bold></th>
<th valign="top" align="center"><bold>Naturality</bold></th>
<th valign="top" align="center"><bold>Diversity</bold></th>
<th valign="top" align="center" colspan="3"><bold>Threat of human interference</bold></th>
<th valign="top" align="center" colspan="3"><bold>Stability</bold></th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">Weight</td>
<td valign="top" align="center">0.2079</td>
<td valign="top" align="center">0.1481</td>
<td valign="top" align="center" colspan="3">0.4065</td>
<td valign="top" align="center">0.2374</td>
<td/>
<td/>
</tr>
<tr style="border-top: thin solid #000000;">
<td valign="top" align="left"><bold>Index</bold></td>
<td valign="top" align="center"><bold>Percentage of vegetation type (PV)</bold></td>
<td valign="top" align="center"><bold>Shannon diversity index (SHDI)</bold></td>
<td valign="top" align="center"><bold>Human disturbance (HD)</bold></td>
<td valign="top" align="center"><bold>Patch density (PD)</bold></td>
<td valign="top" align="center"><bold>Perimeter-Area Fractal Dimension (PAFRAC)</bold></td>
<td valign="top" align="center"><bold>Contagion Index (CONTAG)</bold></td>
<td valign="top" align="center"><bold>Landscape dominance Index (LDI)</bold></td>
<td valign="top" align="center"><bold>Landscape splitting Index (SPLIT)</bold></td>
</tr>
<tr style="border-top: thin solid #000000;">
<td valign="top" align="left">Value</td>
<td valign="top" align="center">21.57</td>
<td valign="top" align="center">1.37</td>
<td valign="top" align="center">0.51</td>
<td valign="top" align="center">10.6</td>
<td valign="top" align="center">1.2</td>
<td valign="top" align="center">67.73</td>
<td valign="top" align="center">0.9</td>
<td valign="top" align="center">9.60</td>
</tr>
<tr>
<td valign="top" align="left">Standardization of value</td>
<td valign="top" align="center">1.566</td>
<td valign="top" align="center">0.064</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0.75</td>
<td valign="top" align="center">0.05</td>
<td valign="top" align="center">5</td>
<td valign="top" align="center">0.03</td>
<td valign="top" align="center">0.68</td>
</tr>
<tr>
<td/>
<td/>
<td/>
<td valign="top" align="center" colspan="3">0.26</td>
<td valign="top" align="center" colspan="3">1.88</td>
</tr>
<tr style="border-top: thin solid #000000;">
<td valign="top" align="left"><bold>Guide line layer</bold></td>
<td valign="top" align="center"><bold>Naturality</bold></td>
<td valign="top" align="center"><bold>Diversity</bold></td>
<td valign="top" align="center"><bold>Threat of human</bold></td>
<td valign="top" align="center"><bold>Stability</bold></td>
<td/>
<td/>
<td/>
<td/>
</tr>
<tr>
<td/>
<td/>
<td/>
<td valign="top" align="center"><bold>interference</bold></td>
<td/>
<td/>
<td/>
<td/>
<td/>
</tr>
<tr style="border-top: thin solid #000000;">
<td valign="top" align="left">Index value</td>
<td valign="top" align="center">1.57</td>
<td valign="top" align="center">0.06</td>
<td valign="top" align="center">0.26</td>
<td valign="top" align="center">1.88</td>
<td/>
<td/>
<td/>
<td/>
</tr>
<tr>
<td valign="top" align="left">Weight</td>
<td valign="top" align="center">0.21</td>
<td valign="top" align="center">0.15</td>
<td valign="top" align="center">0.41</td>
<td valign="top" align="center">0.24</td>
<td/>
<td/>
<td/>
<td/>
</tr>
<tr>
<td valign="top" align="left">Composite evaluation index (CEI)</td>
<td valign="top" align="center">0.89</td>
<td/>
<td/>
<td/>
<td/>
<td/>
<td/>
<td/>
</tr>
</tbody>
</table>
</table-wrap>
<p>A judgment matrix with three level ecological assessment indicators was established in a hierarchical way, which was gauged to reflect the relationship between the affecting factor and its perceived importance. More than 10 experts in the field of landscape or plant diversity assessment were invited to randomly respond to questionnaires &#x0201C;face-to-face&#x0201D; to determine the level of importance: equally important (1), slightly important (3), important (5), obviously important (7), and extremely important (9) (Xu et al., <xref ref-type="bibr" rid="B61">2002</xref>; Wan et al., <xref ref-type="bibr" rid="B46">2005</xref>; Guo et al., <xref ref-type="bibr" rid="B17">2007</xref>). After experts determined the score, we established the judgment matrix and calculated the largest eigenvalue and eigenvector of the matrix as well as the weight of each index value. Regional comprehensive evaluation results are reflected by the following Composite Evaluation Index (CEI) formula (He et al., <xref ref-type="bibr" rid="B20">2001</xref>; Guo and Wang, <xref ref-type="bibr" rid="B18">2005</xref>):</p>
<disp-formula id="E1"><mml:math id="M1"><mml:mtable columnalign="left"><mml:mtr><mml:mtd><mml:mi>C</mml:mi><mml:mi>E</mml:mi><mml:mi>I</mml:mi><mml:mo>=</mml:mo><mml:mfrac><mml:mrow><mml:mn>1</mml:mn></mml:mrow><mml:mrow><mml:mn>4</mml:mn></mml:mrow></mml:mfrac><mml:mstyle displaystyle="true"><mml:munderover accentunder="false" accent="false"><mml:mrow><mml:mo>&#x02211;</mml:mo></mml:mrow><mml:mrow><mml:mi>i</mml:mi><mml:mo>=</mml:mo><mml:mn>1</mml:mn></mml:mrow><mml:mrow><mml:mi>n</mml:mi></mml:mrow></mml:munderover></mml:mstyle><mml:msub><mml:mrow><mml:mi>C</mml:mi></mml:mrow><mml:mrow><mml:mi>i</mml:mi></mml:mrow></mml:msub><mml:msub><mml:mrow><mml:mi>w</mml:mi></mml:mrow><mml:mrow><mml:mi>i</mml:mi></mml:mrow></mml:msub></mml:mtd></mml:mtr></mml:mtable></mml:math></disp-formula>
<p>in which, <italic>C</italic><sub><italic>i</italic></sub> &#x0003D; the score of single evaluation index; <italic>w</italic><sub><italic>i</italic></sub> &#x0003D; the weights of the evaluation indices; <italic>n</italic> &#x0003D; the number of indices. The ecological environmental quality was assessed by the value of CEI according to Zheng et al. (<xref ref-type="bibr" rid="B65">1994</xref>).</p>
<p>We tested the random consistency index (<italic>CR</italic>) of the judgment matrix; if <italic>CR</italic> &#x02264; 0.1, the matrix has a satisfactory consistency, and the weight (w) can be applied (He et al., <xref ref-type="bibr" rid="B20">2001</xref>; Guo and Wang, <xref ref-type="bibr" rid="B18">2005</xref>). The weight of naturality, diversity, stability and threat of human interference is 0.21, 0.15, 0.24, and 0.41 respectively (Table <xref ref-type="table" rid="T5">5</xref>). In the target layer (see the definition in Table <xref ref-type="table" rid="T3">3</xref>), the weight of each indicator is 0.33.</p>
<table-wrap position="float" id="T5">
<label>Table 5</label>
<caption><p>Determination of evaluation index weight of Chaishitan water conservancy project.</p></caption>
<table frame="hsides" rules="groups">
<thead><tr>
<th valign="top" align="left"><bold>A</bold></th>
<th valign="top" align="center"><bold>B1</bold></th>
<th valign="top" align="center"><bold>B2</bold></th>
<th valign="top" align="center"><bold>B3</bold></th>
<th valign="top" align="center"><bold>B4</bold></th>
<th valign="top" align="center"><bold>W<sub>i</sub></bold></th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">B1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">2</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1/3</td>
<td valign="top" align="center">0.21</td>
</tr>
<tr>
<td valign="top" align="left">B2</td>
<td valign="top" align="center">1/2</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1/3</td>
<td valign="top" align="center">0.15</td>
</tr>
<tr>
<td valign="top" align="left">B3</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0.24</td>
</tr>
<tr>
<td valign="top" align="left">B4</td>
<td valign="top" align="center">3</td>
<td valign="top" align="center">3</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0.41</td>
</tr>
</tbody>
</table>
</table-wrap>
</sec>
<sec>
<title>Plant diversity analysis before modeling</title>
<p>The arrangement order of the families is determined by Li (<xref ref-type="bibr" rid="B23">1996</xref>) and Wu (<xref ref-type="bibr" rid="B58">1991</xref>). Simpson, Shannon and Pielou indices were calculated to evaluate the diversity of trees, shrubs, and herb species in each sampling site. The indices were calculated as follows:
<list list-type="order">
<list-item><p>The Simpson diversity index (<italic>D</italic>) (Simpson, <xref ref-type="bibr" rid="B38">1949</xref>);
<disp-formula id="E2"><mml:math id="M2"><mml:mtable columnalign="left"><mml:mtr><mml:mtd><mml:mtable style="text-align:axis;" equalrows="false" columnlines="none none none none none none none none none" equalcolumns="false" class="array"><mml:mtr><mml:mtd><mml:mi>D</mml:mi><mml:mo>=</mml:mo><mml:mn>1</mml:mn><mml:mo>-</mml:mo><mml:mstyle displaystyle="true"><mml:munderover accentunder="false" accent="false"><mml:mrow><mml:mo>&#x02211;</mml:mo></mml:mrow><mml:mrow><mml:mi>i</mml:mi><mml:mo>=</mml:mo><mml:mn>1</mml:mn></mml:mrow><mml:mrow><mml:mi>S</mml:mi></mml:mrow></mml:munderover></mml:mstyle><mml:msubsup><mml:mrow><mml:mi>P</mml:mi></mml:mrow><mml:mrow><mml:mi>i</mml:mi></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow></mml:msubsup></mml:mtd><mml:mtd><mml:msubsup><mml:mrow><mml:mi>P</mml:mi></mml:mrow><mml:mrow><mml:mi>i</mml:mi></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow></mml:msubsup><mml:mo>=</mml:mo><mml:mfrac><mml:mrow><mml:msub><mml:mrow><mml:mi>n</mml:mi></mml:mrow><mml:mrow><mml:mi>i</mml:mi></mml:mrow></mml:msub><mml:mrow><mml:mo stretchy="false">(</mml:mo><mml:mrow><mml:msub><mml:mrow><mml:mi>n</mml:mi></mml:mrow><mml:mrow><mml:mi>i</mml:mi></mml:mrow></mml:msub><mml:mo>-</mml:mo><mml:mn>1</mml:mn></mml:mrow><mml:mo stretchy="false">)</mml:mo></mml:mrow></mml:mrow><mml:mrow><mml:mi>N</mml:mi><mml:mrow><mml:mo stretchy="false">(</mml:mo><mml:mrow><mml:mi>N</mml:mi><mml:mo>-</mml:mo><mml:mn>1</mml:mn></mml:mrow><mml:mo stretchy="false">)</mml:mo></mml:mrow></mml:mrow></mml:mfrac></mml:mtd></mml:mtr></mml:mtable></mml:mtd></mml:mtr></mml:mtable></mml:math></disp-formula></p></list-item>
<list-item><p>The Shannon index (e-base) <italic>H</italic>&#x02032;<sub>e</sub> (Shannon, <xref ref-type="bibr" rid="B39">1948</xref>);
<disp-formula id="E3"><mml:math id="M4"><mml:mtable columnalign="left"><mml:mtr><mml:mtd><mml:msub><mml:mrow><mml:mrow><mml:mi>H</mml:mi></mml:mrow><mml:mrow><mml:mo>&#x02032;</mml:mo></mml:mrow></mml:mrow><mml:mrow><mml:mi>e</mml:mi></mml:mrow></mml:msub><mml:mo>=</mml:mo><mml:mo>-</mml:mo><mml:mstyle displaystyle="true"><mml:munderover accentunder="false" accent="false"><mml:mrow><mml:mo>&#x02211;</mml:mo></mml:mrow><mml:mrow><mml:mi>i</mml:mi><mml:mo>-</mml:mo><mml:mn>1</mml:mn></mml:mrow><mml:mrow><mml:mi>S</mml:mi></mml:mrow></mml:munderover></mml:mstyle><mml:msub><mml:mrow><mml:mi>P</mml:mi></mml:mrow><mml:mrow><mml:mi>i</mml:mi></mml:mrow></mml:msub><mml:mtext class="textrm" mathvariant="normal">ln</mml:mtext><mml:msub><mml:mrow><mml:mi>P</mml:mi></mml:mrow><mml:mrow><mml:mi>i</mml:mi></mml:mrow></mml:msub></mml:mtd></mml:mtr></mml:mtable></mml:math></disp-formula></p></list-item>
<list-item><p>The Pielou evenness index (<italic>J</italic>) (Pielou, <xref ref-type="bibr" rid="B35">1966</xref>):
<disp-formula id="E4"><mml:math id="M5"><mml:mtable columnalign="left"><mml:mtr><mml:mtd><mml:msub><mml:mrow><mml:mi>J</mml:mi></mml:mrow><mml:mrow><mml:mi>e</mml:mi></mml:mrow></mml:msub><mml:mo>=</mml:mo><mml:mfrac><mml:mrow><mml:msub><mml:mrow><mml:mrow><mml:mi>H</mml:mi></mml:mrow><mml:mrow><mml:mo>&#x02032;</mml:mo></mml:mrow></mml:mrow><mml:mrow><mml:mi>e</mml:mi></mml:mrow></mml:msub></mml:mrow><mml:mrow><mml:msub><mml:mrow><mml:mrow><mml:mi>H</mml:mi></mml:mrow><mml:mrow><mml:mo>&#x02032;</mml:mo></mml:mrow></mml:mrow><mml:mrow><mml:mo class="qopname">max</mml:mo></mml:mrow></mml:msub></mml:mrow></mml:mfrac></mml:mtd></mml:mtr></mml:mtable></mml:math></disp-formula></p></list-item>
</list></p>
<p>In the formulae 1 through 3, <italic>P</italic><sub>i</sub> &#x0003D; <italic>n</italic><sub><italic>i</italic></sub><italic>/N</italic>, where <italic>n</italic><sub><italic>i</italic></sub> is the number of an individual species <italic>I, N</italic> is individual number of all species, while <italic>H</italic>&#x00027;<sub>max</sub>is the maximum Shannon index. If <italic>D</italic> &#x0003D; 0, there are no species in the plot.</p>
<p>SPSS (Statistical Product and Service Solutions) was used for statistical analyses to test the significance of differences, i.e., there is a significant difference between two variables if <italic>p</italic> &#x0003C; 0.05 (and not if <italic>p</italic> &#x0003E; 0.05).</p>
</sec>
</sec>
</sec>
<sec sec-type="results" id="s3">
<title>Results</title>
<sec>
<title>Land use and landscape pattern changes before and after WCP established</title>
<sec>
<title>Land use changes</title>
<p>The total area of the WCP was 486,985 km<sup>2</sup>, including Urban and industrial land, Farmland, Orchard, Forest land, and Waters/water conservancy facilities (Table <xref ref-type="table" rid="T1">1</xref>). The land use did not change significantly once the WCP was established. Minor land use changes were detected before and immediately after the establishment of the Chaishitan WCP. The greatest decline was 0.079% for Farmland (0.161 km<sup>2</sup>, the amount of the decreased area, similarly hereinafter), then 0.066% for Forestland (0.104 km<sup>2</sup>). The least decreased is 0.020% (0.003 km<sup>2</sup>) for water bodies (Table <xref ref-type="table" rid="T1">1</xref>, Figure <xref ref-type="fig" rid="F2">2</xref>). The naturality and diversity decreased 11.18 and 10.16%, respectively. In the guide line layer (see the definitions in Table <xref ref-type="table" rid="T3">3</xref>), a layer that includes naturality, diversity, stability, and threat of human interference (see Tables <xref ref-type="table" rid="T4A">4A</xref>,<xref ref-type="table" rid="T4B">B</xref>). However, the threat of human interference index and ecosystem stability increased by 1.32 and 1.59%, respectively. The CEI decreased by 3.65% (Tables <xref ref-type="table" rid="T4A">4A</xref>,<xref ref-type="table" rid="T4B">B</xref>).</p>
<fig id="F2" position="float">
<label>Figure 2</label>
<caption><p>The land-use changes before <bold>(A)</bold> and after <bold>(B)</bold> the Chaishitan water conservancy established.</p></caption>
<graphic xlink:href="fevo-05-00060-g0002.tif"/>
</fig>
</sec>
<sec>
<title>Landscape pattern changes</title>
<p>The landscape changes after the WCP established were minor. The value of some landscape pattern indices decreased by about 10% while the value of other indices increased by about 3%. In the index layer (see Tables <xref ref-type="table" rid="T4A">4A</xref>,<xref ref-type="table" rid="T4B">B</xref>), we found that Percentage of Vegetation type (PV), Shannon Diversity Index (SHDI), Perimeter-Area Fractal Dimension (PAFRAC) and Landscape dominance Index (LDI) decreased by 11.18, 10.16, 11.70, and 5.69%, respectively; however, we found that Patch density (PD), Contagion Index (CONTAG) and Landscape splitting Index (SPLIT) increased by 2.37, 0.75, and 8.66%, respectively (Tables <xref ref-type="table" rid="T4A">4A</xref>,<xref ref-type="table" rid="T4B">B</xref>).</p>
<p>Class-level analysis indicates the structural characteristics for each land use type and can reveal the land use change features and trends. Eight class metrics; namely, NP, PD, ED, LPI, PLAND, LSI, SHAPE_MN, and FRAC_AM (see the abbreviations in the Methods) were selected to perform the land use type change analysis in this research and the statistical results are shown in Table <xref ref-type="table" rid="T6">6</xref>, indicating changes in the landscape structure of each cover type.</p>
<table-wrap position="float" id="T6">
<label>Table 6</label>
<caption><p>Statistics of pattern metrics in class level.</p></caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th valign="top" align="left"><inline-graphic xlink:href="fevo-05-00060-i0001.tif"/></th>
<th valign="top" align="center"><bold>NP</bold></th>
<th valign="top" align="center"><bold>PD</bold></th>
<th valign="top" align="center"><bold>ED</bold></th>
<th valign="top" align="center"><bold>LPI</bold></th>
<th valign="top" align="center"><bold>PLAND</bold></th>
<th valign="top" align="center"><bold>LSI</bold></th>
<th valign="top" align="center"><bold>SHAPE_MN</bold></th>
<th valign="top" align="center"><bold>FRAC_AM</bold></th>
<th/>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">Urban and industrial land</td>
<td valign="top" align="left">159</td>
<td valign="top" align="center">0.7358</td>
<td valign="top" align="center">19.1538</td>
<td valign="top" align="center">2.4414</td>
<td valign="top" align="center">9.6234</td>
<td valign="top" align="center">20.8506</td>
<td valign="top" align="center">1.5789</td>
<td valign="top" align="center">1.1245</td>
<td/>
</tr>
<tr>
<td/>
<td/>
<td valign="top" align="center">187</td>
<td valign="top" align="center">0.8654</td>
<td valign="top" align="center">19.5661</td>
<td valign="top" align="center">2.2053</td>
<td valign="top" align="center">9.6148</td>
<td valign="top" align="center">23.994</td>
<td valign="top" align="center">1.8384</td>
<td valign="top" align="center">1.1747</td>
</tr>
<tr style="border-top: thin solid #000000;">
<td valign="top" align="left">Farmland</td>
<td valign="top" align="left">204</td>
<td valign="top" align="center">0.9441</td>
<td valign="top" align="center">74.8151</td>
<td valign="top" align="center">29.9419</td>
<td valign="top" align="center">49.704</td>
<td valign="top" align="center">40.9085</td>
<td valign="top" align="center">2.0482</td>
<td valign="top" align="center">1.3375</td>
<td/>
</tr>
<tr>
<td/>
<td/>
<td valign="top" align="center">311</td>
<td valign="top" align="center">1.4393</td>
<td valign="top" align="center">78.4572</td>
<td valign="top" align="center">20.5175</td>
<td valign="top" align="center">49.6296</td>
<td valign="top" align="center">42.8379</td>
<td valign="top" align="center">1.9444</td>
<td valign="top" align="center">1.3128</td>
</tr>
<tr style="border-top: thin solid #000000;">
<td valign="top" align="left">Orchard</td>
<td valign="top" align="left">1003</td>
<td valign="top" align="center">4.6417</td>
<td valign="top" align="center">37.2329</td>
<td valign="top" align="center">0.6538</td>
<td valign="top" align="center">10.7541</td>
<td valign="top" align="center">42.5893</td>
<td valign="top" align="center">1.5399</td>
<td valign="top" align="center">1.1328</td>
<td/>
</tr>
<tr>
<td/>
<td/>
<td valign="top" align="center">1069</td>
<td valign="top" align="center">4.9472</td>
<td valign="top" align="center">37.8812</td>
<td valign="top" align="center">0.6538</td>
<td valign="top" align="center">10.7404</td>
<td valign="top" align="center">43.3495</td>
<td valign="top" align="center">1.5321</td>
<td valign="top" align="center">1.1312</td>
</tr>
<tr style="border-top: thin solid #000000;">
<td valign="top" align="left">Other kinds of forest land</td>
<td valign="top" align="left">220</td>
<td valign="top" align="center">1.0181</td>
<td valign="top" align="center">10.8556</td>
<td valign="top" align="center">0.6994</td>
<td valign="top" align="center">3.8766</td>
<td valign="top" align="center">20.8506</td>
<td valign="top" align="center">1.5789</td>
<td valign="top" align="center">1.1245</td>
<td/>
</tr>
<tr>
<td/>
<td/>
<td valign="top" align="center">249</td>
<td valign="top" align="center">1.1523</td>
<td valign="top" align="center">11.2246</td>
<td valign="top" align="center">0.5563</td>
<td valign="top" align="center">3.8688</td>
<td valign="top" align="center">21.5579</td>
<td valign="top" align="center">1.5639</td>
<td valign="top" align="center">1.1189</td>
</tr>
<tr style="border-top: thin solid #000000;">
<td valign="top" align="left">Forest land</td>
<td valign="top" align="left">423</td>
<td valign="top" align="center">1.9576</td>
<td valign="top" align="center">36.0051</td>
<td valign="top" align="center">3.4285</td>
<td valign="top" align="center">20.0835</td>
<td valign="top" align="center">30.9984</td>
<td valign="top" align="center">1.6884</td>
<td valign="top" align="center">1.1913</td>
<td/>
</tr>
<tr>
<td/>
<td/>
<td valign="top" align="center">506</td>
<td valign="top" align="center">2.3417</td>
<td valign="top" align="center">38.2225</td>
<td valign="top" align="center">3.082</td>
<td valign="top" align="center">24.0385</td>
<td valign="top" align="center">32.6911</td>
<td valign="top" align="center">1.6657</td>
<td valign="top" align="center">1.1819</td>
</tr>
<tr style="border-top: thin solid #000000;">
<td valign="top" align="left">Water</td>
<td valign="top" align="left">165</td>
<td valign="top" align="center">0.7636</td>
<td valign="top" align="center">6.4574</td>
<td valign="top" align="center">0.2057</td>
<td valign="top" align="center">1.9585</td>
<td valign="top" align="center">17.3578</td>
<td valign="top" align="center">1.5371</td>
<td valign="top" align="center">1.1096</td>
<td/>
</tr>
<tr>
<td/>
<td/>
<td valign="top" align="center">179</td>
<td valign="top" align="center">0.8284</td>
<td valign="top" align="center">6.5705</td>
<td valign="top" align="center">0.2057</td>
<td valign="top" align="center">1.9558</td>
<td valign="top" align="center">17.6657</td>
<td valign="top" align="center">1.5211</td>
<td valign="top" align="center">1.1054</td>
</tr>
<tr style="border-top: thin solid #000000;">
<td valign="top" align="left">Water conservancy facilities Land</td>
<td valign="top" align="left">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td/>
</tr>
<tr>
<td/>
<td/>
<td valign="top" align="center">16</td>
<td valign="top" align="center">0.074</td>
<td valign="top" align="center">7.7765</td>
<td valign="top" align="center">0.0706</td>
<td valign="top" align="center">0.1521</td>
<td valign="top" align="center">73.2723</td>
<td valign="top" align="center">13.3522</td>
<td valign="top" align="center">1.6196</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p><italic>Notes: NP, Number of Patches; PD, Patch Density; ED, Edge Density; LPI, Largest Patch Index; PLAND, Percentage of Landscape; LSI, Landscape Shape Index; SHAPE_MN, Shape Index (Mean); FRAC_AM, Fractal Dimension Index (Area-Weighted)</italic>.</p>
</table-wrap-foot>
</table-wrap>
</sec>
</sec>
<sec>
<title>Main vegetation types</title>
<p>Five kinds of main vegetation types were found in the field:</p>
<sec>
<title>Secondary broadleaf forest (SF)</title>
<p>Secondary broadleaf forest is the main forest type commonly found in high altitudes; its dominant species includes <italic>Pinus yunnanensis</italic> Franch., <italic>Quercus variabilis</italic> Bl. and <italic>Cupressus duclouxiana</italic> Hickel, <italic>Cyclobalanopsis glauca</italic> (Thunb.) Oerst., along with <italic>Acer buergerianum</italic> Miq (Figure <xref ref-type="fig" rid="F3">3</xref>).</p>
<fig id="F3" position="float">
<label>Figure 3</label>
<caption><p>The main land use types in Chaishitan irrigation region.</p></caption>
<graphic xlink:href="fevo-05-00060-g0003.tif"/>
</fig>
</sec>
<sec>
<title>Eucalyptus plantations (EP)</title>
<p>Eucalyptus plantations [the main species is <italic>Eucalyptus globulus</italic> subsp. <italic>maidenii</italic> (F. Muell.) Kirkpatr.] were widely distributed in the roadside, hillside, dry land and farmland. Most of them are disjunct and distributed in small areas.</p>
</sec>
<sec>
<title>Farmland (FL)</title>
<p>Farmland mainly comprised dry areas growing corn, greenhouses containing common crops [such as <italic>Panax notoginseng</italic> (Burkill) F. H. Chen ex C. H. Chow, <italic>Nicotiana tabacum</italic> Linn.], and lowlands with good irrigation growing rice.</p>
</sec>
<sec>
<title>Orchards (OR)</title>
<p>There are a large number of orchards, including those growing <italic>Castanea mollissima</italic> Bl., <italic>Cerasus pseudocerasus</italic> (Lindl.) G. Don (chestnut), and <italic>Vaccinium corymbosum</italic> L. (blueberry) etc. Most orchards cultivated one or two fruit trees. There were some weeds (such as <italic>Eupatorium adenophorum</italic> Bidens) living in the understory of orchards.</p>
</sec>
<sec>
<title>Wasteland (WL)</title>
<p>Marsh was regarded as the wasteland in this study, which was found near rice fields. Natural marsh vegetation was rarely found in our field work. The main plant species are invasive alien species such as <italic>Alternanthera philoxeroides</italic> (Mart.) Griseb., and <italic>Eupatorium adenophorum</italic> Hort. Berol. ex Kunth, mixed with other typical marsh plants such as <italic>Equisetum ramosissimum</italic> subsp. debile (Roxb. ex Vauch.) Hauke.</p>
</sec>
</sec>
<sec>
<title>Floristic composition</title>
<p>There was a high vascular plant taxonomic diversity (over 1,000 plant species) found in the investigated fields. A total of 1076 vascular plant species (including subspecies, varieties and form, Supplementary Material <xref ref-type="supplementary-material" rid="SM1">B</xref>) were identified in the Chaishitan irrigation region, belonging to 165 families and 647 genera (Supplementary Material <xref ref-type="supplementary-material" rid="SM1">B</xref>). Of these, 23 species (13 families and 16 genera) belong to pteridophytes, accounting for 0.88% of all pteridophyte species in China, whereas 60 species (8 families and 31 genera) are gymnosperms, accounting for 0.8% of gymnosperms species in China. The remaining 993 species (144 families and 600 genera) are angiosperms, accounting for 3.58% of all angiosperms in China. Angiosperm species accounts for 92.29% of all vascular species.</p>
<p>All 1,076 vascular species could be divided into three categories: (1) 295 species (87 families and 226 genera) are wild native species; (2) 748 species (138 families and 462 genera) alien are cultivated species, including vegetables, fruit, aromatic plant, cash crops, and a very large number of garden plant species; (3) 33 species (16 families and 30 genera) are alien invasive species, such as <italic>Eupatorium adenophorum</italic> Hort. Berol. ex Kunth, <italic>Conyza canadensis</italic> L. and <italic>Ageratum conyzoides</italic> Sieber ex Steud.</p>
<p>Poaceae, Fabaceae, and Rosaceae accounted for the most families in Chaishitan irrigation region. Poaceae was represented by the most species (70 species, 48 genera), in which 46 species are wild native species. Fabaceae and Rosaceae were represented by the second (62 species and 36 genera) and the third highest number of species (61 species and 21 genera), respectively. In addition, 49 families were represented by only one species.</p>
</sec>
<sec>
<title>Plant diversity index</title>
<p>Plant taxonomic diversity indices (i.e., <italic>d, He</italic>&#x02032; and <italic>Je</italic>) differed in different vegetation types. In tree layer, index <italic>d, He</italic>&#x02032; and <italic>Je</italic> are the highest in farmland compared to the other four vegetation types (Figure <xref ref-type="fig" rid="F4">4A</xref>). In shrub layer, <italic>d</italic> and <italic>He</italic>&#x02032; are the highest in Wasteland (WL); however, <italic>Je</italic> is the highest in Farmland (FL) (Figure <xref ref-type="fig" rid="F4">4B</xref>). In the herb layer, <italic>d, He</italic>&#x02032; and <italic>Je</italic> are the highest in the secondary needle- and broad-leaved mixed forest (SF) (Figure <xref ref-type="fig" rid="F4">4C</xref>).</p>
<fig id="F4" position="float">
<label>Figure 4</label>
<caption><p>The plant diversity <italic>d, He</italic>&#x02032;, and <italic>Je</italic> in tree <bold>(A)</bold>, shrub <bold>(B)</bold>, and herb <bold>(C)</bold> layer. SF, Secondary evergreen needle- and broad-leaves mixed forest; FL, Farmland; EP, Eucaluptus plantation; OR, Orchard; WL, Wasteland.</p></caption>
<graphic xlink:href="fevo-05-00060-g0004.tif"/>
</fig>
</sec>
<sec>
<title>Rare and protected plant species</title>
<p>Some rare or protected plant species were found in the Greenhouses or Nurseries; i.e., cultivars were mainly for ornamental or medicinal use, and were not wild species, such as <italic>Alsophila spinulosa</italic> (Wall. ex Hook.) R. M. Tryon, <italic>Ginkgo biloba</italic> Linn., <italic>Juglans mandshurica</italic> Maxim., <italic>Pseudolarix amabilis</italic> (Nelson) Rehd., <italic>Glyptostrobus pensilis</italic> (Staunt.) Koch, <italic>Metasequoia glyptostroboides</italic> Hu et Cheng, <italic>Taxus wallichiana</italic> var. <italic>chinensis</italic> (Pilg.) Florin, <italic>Liriodendron chinense</italic> (Hemsl.) Sargent., <italic>Pachylarnax sinica</italic> (Law) N. H Xia et C. Y. Wu, <italic>Phoebe zhennan</italic> S. Lee et F. N. Wei, <italic>Fagopyrum dibotrys</italic> (D. Don) Hara, <italic>Davidia involucrate</italic> Baill., <italic>Sinojackia xylocarpa</italic> Hu, and <italic>Kolkwitzia amabilis</italic> Graebn. were found in our field investigation. These species are listed as national key protected plants species. However, as these were not wild species, their genetic diversity is lower. Furthermore, as there were a large number of individuals of these species, they have a lower conservation value. There were numerous clones of the protected species in the greenhouses and nurseries.</p>
</sec>
</sec>
<sec sec-type="discussion" id="s4">
<title>Discussion</title>
<p>Determining a project&#x00027;s evaluation scope is one of the key issues in the EIA procedures (TGEIAEI (Technical Guidelines for Environmental Impact Assessment Ecological Impact), <xref ref-type="bibr" rid="B44">2011</xref>; Liang, <xref ref-type="bibr" rid="B25">2015</xref>; Tan et al., <xref ref-type="bibr" rid="B43">2015</xref>). According to TGEIAEI (Technical Guidelines for Environmental Impact Assessment Ecological Impact) (<xref ref-type="bibr" rid="B44">2011</xref>), EIA should fully embody the ecological integrity, covering the directly and indirectly affected areas of all activities of the assessment projects. The scope of evaluation work should be determined by the impact of the evaluation project on ecological factors, the impact degree and the interaction among ecological factors. We should consider the relationships between the evaluation projects and the climatic processes, hydrological processes, and biological processes. We should take the complete climatic units, hydrological units, eco-units and geographic boundaries as reference boundaries. However, there was no specific evaluation scope regulated in TGEIAEI (Technical Guidelines for Environmental Impact Assessment Ecological Impact) (<xref ref-type="bibr" rid="B44">2011</xref>) mainly because of the reasons as follows: First, China has a broad geography with diverse ecosystem types, and the projects are complex. Second, different industry guides have clearly defined the scope of the evaluation work; Third, according to the statistics on previous construction projects, most ecological impact assessments have not been carried out in accordance with the evaluation scope recommended by the original guidelines (Liang, <xref ref-type="bibr" rid="B25">2015</xref>; Tan et al., <xref ref-type="bibr" rid="B43">2015</xref>).</p>
<p>In this study, given that the low water temperature and salinity will affect the fish breeding ten kilometers downstream, our evaluation scope included those areas. In the public involvement procedure, we drafted questionnaires and posted them in the places of greatest population concentration to ask local people their opinions and suggestions about biodiversity changes, which helps local biodiversity protection in the future.</p>
<p>&#x0201C;Before-and-after&#x0201D; methods have been applied to previous projects in China. However, some EIA projects did not have systematic (i.e., complete and comprehensive) environment impact assessments for non-natural reserve areas once the project received approval from the government departments, e.g., some assessments only evaluated the status for non-natural reserve areas or neglected plant diversity evaluation in these areas. In this study, we not only evaluated the status before the project was established, but we also systematically evaluated future conditions based on modeling.</p>
<sec>
<title>The impact of chaishitan WCP on local landscape</title>
<p>The accuracy of remote sensing depends on the data extraction process (Zhu et al., <xref ref-type="bibr" rid="B66">2016</xref>). In our study, the remote sensing image was from OLI data based on Landsat 8: its resolution is 30 m. Thus, the accuracy defines the meaningfulness of the data. For example, the percentage decline of Farmland and Forestland was 0.079 and 0.066%, respectively. However, if the sensing accuracy is only 70%, these changes are not meaningful at all, because the simple classification error is much greater than the magnitude of changes. Unfortunately, high resolution images are either simply not available or prohibitively expensive, as are images from SPOT (Satellite Pour l&#x00027;Observation de la Terre) or Quickbird. In this study, based on OLI data from Landsat 8, the CEI before and after Chaishitan WCP was established is 0.92 and 0.89, respectively. Although the CEI decreased by 0.03, both CEI values indicate that the ecological state is healthy, according to the criteria in Zheng et al. (<xref ref-type="bibr" rid="B65">1994</xref>). These values indicate that the Chaishitan WCP will make the CEI decrease slightly, but will not decrease the rank of the overall ecological quality of the Chaishitan region.</p>
<p>Environment Impact Assessments are often conducted before a project is established, can lead to a lack of land use/cover assessments after establishment. In this study, we predicted the changes of land use/cover based on the data from planning diagrams, and found that the structure of landscapes could change after the establishment of the Chaishitan WCP. Specifically, PV, SHDI, PAFRAC, and LDI decreased, while PD, CONTAG, and SPLIT increased, indicating increased landscape fragmentation along with decreases in landscape diversity. The Chaishitan WCP establishment could result in land use change and therefore most landscape indices would change; e.g., PV decreases because the channels or canals would occupy the areas normally containing some natural vegetation, resulting in natural vegetation decreases. On the other hand, the Chaishitan WCP establishment would make the landscape more fragmented, and therefore, the SPLIT will increase due to the generation of more patches.</p>
<sec>
<title>Shape feature analysis</title>
<p>We can see from the column of SHAPE_MN (Table <xref ref-type="table" rid="T5">5</xref>) that only the land use of urban and industrial land increased, while others decreased, especially for orchards with the least change. This indicates that the shape for urban and industrial land has become more complicated, while other land use types have become more simple or regular after the implementation of the water conservancy facilities. This phenomenon might be caused by the design of this water conservancy facility, which endeavors to have the least disruption to farmland as possible. The situation for SHAPE_MN is almost the same as the index of FRAC_AM, which represents the self-correlation of patches. The value of FRAC_AM is negatively proportional to the impact of human activities. All the FRAC_AM values are very small, which means that all of these land use types are easily affected by human activities.</p>
</sec>
<sec>
<title>Landscape domination analysis</title>
<p>PLAND and LPI are often used to identify the dominance of the land use type in the whole landscape (O&#x00027;Neill et al., <xref ref-type="bibr" rid="B33">1988</xref>; Wu, <xref ref-type="bibr" rid="B55">2000</xref>). In this analysis, these two indices indicated that farmland would remain as the dominant land use type after the construction of the water conservancy facilities.</p>
</sec>
<sec>
<title>Landscape fragmentation analysis</title>
<p>PD and ED are generally combined to analyze the degree of fragmentation for each land use type (O&#x00027;Neill et al., <xref ref-type="bibr" rid="B33">1988</xref>; Wu, <xref ref-type="bibr" rid="B55">2000</xref>; Wen et al., <xref ref-type="bibr" rid="B52">2008</xref>). By comparing the values of these two indices for all of these land use types, it can be concluded orchard and water bodies have the largest and least degree of fragmentation, respectively. After performing the construction of water conservancy facilities, the degree of fragmentation for all the land used types increased. This means that the water conservation facilities have caused fragmentation of each land use type.</p>
</sec>
</sec>
<sec>
<title>The impacts of the chaishitan WCP on plant species</title>
<p>In this study, the Chaishitan WCP is predicted to change the area values of different land types (Table <xref ref-type="table" rid="T1">1</xref>); however, the land use change should not decrease plant diversity because of the three following reasons: first, the change in farmland area (0.161 km<sup>2</sup>) and forest land area (0.104 km<sup>2</sup>) is limited (Table <xref ref-type="table" rid="T1">1</xref>). Second, as shown in our field investigation, the Chaishitan project will not occupy natural forest, for all affected forests are secondary forests, plantations or orchards (Tables <xref ref-type="table" rid="T4A">4A</xref>,<xref ref-type="table" rid="T4B">B</xref>). The plant diversity in the farmland and artificial forestland is not high, as most species are cultivated species or alien species, and the species in the forest are widely distributed in that region. Finally, there is no special species sensitive to local habit (Supplementary Material <xref ref-type="supplementary-material" rid="SM1">B</xref>). On the contrary, Chaishitan WCP might increase the number of local plant species in the long term because once the project is in operation, the human interventions will be reduced, which may create more beneficial habitat or niche for more species due to sufficient water supply.</p>
<p>From a whole ecosystem perspective, the ability of the ecosystem to resist alien species invasion, plant diseases and pest invasion will increase because of sufficient water supply. In the long term, ecosystem stability will depend on multiple factors (e.g., improved soil texture), and the temporary biodiversity decrease should not be reflected in the CEI decrease in the future, because the ecosystem resilience will improve the plant diversity.</p>
<p>An Impact Assessment is an important tool for conservation and sustainable use of biodiversity (IAIA, <xref ref-type="bibr" rid="B21">2005</xref>). There is a growing interest in promoting biodiversity consideration in impact assessments (e.g., IAIA, <xref ref-type="bibr" rid="B21">2005</xref>; CBD (Convention on Biological Diversity), <xref ref-type="bibr" rid="B6">2006</xref>). In order to support this trend, many guidelines and tools have been developed (e.g., World Bank, <xref ref-type="bibr" rid="B54">2000</xref>; OECD (Organisation for Economic Cooperation Development), <xref ref-type="bibr" rid="B32">2002</xref>). Wegner et al. (<xref ref-type="bibr" rid="B51">2005</xref>) points out the diversity in definitions and approaches to its assessment among EIA practitioners. Geneletti et al. (<xref ref-type="bibr" rid="B15">2003</xref>) further argued that accounting for uncertainty in biodiversity impact assessment&#x02014;in data, methodologies, and value judgments provided by the experts&#x02014;is important. Gontier et al. (<xref ref-type="bibr" rid="B16">2006</xref>) also address the gap between research in prediction tools and current practice in biodiversity assessment within environmental assessment. Post-project-analysis (PPA) refers to a method and system of tracing, monitoring and confirmatory assessing the environmental impact of constructed projects and the efficiency of preventive measures, as well as proposing remedial plans or measures, aiming to achieve the coordination between project construction and environment (Division of Bill, Resources and Environmental Commission of National People&#x00027;s Congress (DBRECNP), <xref ref-type="bibr" rid="B12">2003</xref>). At present, the PPA in environmental impact in China is basically at the stage of discussing the concept, indicators, methods, content and procedure of the assessment as well as developing case studies (Li et al., <xref ref-type="bibr" rid="B24">1997</xref>; Shen et al., <xref ref-type="bibr" rid="B40">2005</xref>; Cai et al., <xref ref-type="bibr" rid="B4">2007</xref>), while post-project analysis in construction projects is not yet widely conducted. In this study, our assessment was conducted before the project was established, i.e., Before-Project-Analysis (BPA), which could maximally decrease the environmental risk the project brings. In this study, we used modeling predict the land use and plant diversity change immediately after the project established, while as discussed previously, plant could restore to its mature state in a long term (e.g., 5 years, 10 years), therefore, it seems that another comprehensive field investigation is needed to understand what the irrigation system will change. The rare or endangered plant species were found in the greenhouse or nursery, and no wild individuals were found in the investigated field. Furthermore, the irrigation project will provide sufficient water once it is established, which will enrich local plant diversity.</p>
<p>The loss of biodiversity in non-natural reserves is much higher than the loss rate of nature reserves due to over-exploitation, habitat loss, etc. in China (Xia et al., <xref ref-type="bibr" rid="B59">2005</xref>). In this study, we used the Yiliang WCP as an example and we set up hundreds of plant diversity plots to systematically investigate the plant diversity, abundance, and conservation status. Our real intent is to generate more attention the landscape and plant diversity of non-natural reserve areas in China.</p>
</sec>
</sec>
<sec sec-type="conclusions" id="s5">
<title>Conclusion</title>
<p>Systematic environment impact assessment for non-natural reserve areas should be conducted with the same weight as assessments for natural reserve areas, because the baseline for such non-natural areas is needed and too easily neglected. Ignoring these baselines could create data gaps, which in turn could impede future project establishment or environment assessment in a long term. In this study, we proposed a comprehensive evaluation system to assess the potential ecological and environmental consequences of WCP. Our ecological evaluation results showed that the Chaishitan water conservancy project may initially fragment landscape patterns and deteriorate the local ecological conditions. Furthermore, the plant diversity will decrease when the WCP has been constructed. However, the maps of channels indicated that the local diversity will likely rebound and increase in the long term, which will provide beneficial habitat for local species. Therefore, it is appropriate to establish a WCP in Chaishitan irrigation region as long as we model and understand the ecological and environmental consequences of the WCP using well-established socioeconomic evaluation systems.</p>
</sec>
<sec id="s6">
<title>Author contributions</title>
<p>HW and ZZ conceived this idea, HW, GC, and QL did the field work, remote sensing image interpretation and data analyses, GC and KZ helped creating the figures. HW, CR, and SQ revised the manuscript. All authors contributed to the manuscript.</p>
<sec>
<title>Conflict of interest statement</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
</sec>
</body>
<back>
<sec sec-type="supplementary-material" id="s7">
<title>Supplementary material</title>
<p>The Supplementary Material for this article can be found online at: <ext-link ext-link-type="uri" xlink:href="http://journal.frontiersin.org/article/10.3389/fevo.2017.00060/full#supplementary-material">http://journal.frontiersin.org/article/10.3389/fevo.2017.00060/full#supplementary-material</ext-link></p>
<supplementary-material xlink:href="Table1.DOC" id="SM1" mimetype="application/msword" xmlns:xlink="http://www.w3.org/1999/xlink"/>
</sec>
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<fn-group>
<fn fn-type="financial-disclosure">
<p><bold>Funding.</bold> This study was funded by National Scientific Foundation of China (31660055 and 31660074), the initial fund from Hainan University (kyqd1633), the fund from China Ministry of Science and Technology (grant number 2012BAJ14B03-6) and Beijing Municipal Education Commission (grant number UDC2016050100).</p>
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