The lacustrine cores used in this study were retrieved from Lake Meihua (121° 43.57′, 24° 38.35′; 52 ha; 50 m a.s.l.), which is situated on the southern edge of the Ilan Plain, northeastern Taiwan. The Ilan Plain is a triangular-shaped alluvial-fan delta, facing the Pacific Ocean to the east. Two major mountain ranges border the northern and southern flanks, the Hsueshan Range and the Central Range, respectively (Figure 1A). The basement rock here is mainly composed of Tertiary argillite, slate and metasandstone (Ho, 1986). The continuous subsidence associated with the westward propagation of the Okinawa Trough has provided over 120 m of sedimentary accommodation space in the Ilan basin since the LGM (Wei et al., 2003; Chen et al., 2009). The high sedimentation rates are the result of intense rain-induced erosion of surrounding mountain areas; the Lanyang River supplies about 17 Mt of suspended sediments per year (Dadson et al., 2003). The position at the southern edge of the Ilan Plain results in Lake Meihua being surrounded by hills, with the only opening toward the Ilan Plain to the north (Figure 1B). A normal faulting system relating to the tectonic subsidence along the mountain front (Shyu et al., 2005) together with rapid river aggradation at the northern shore of the lake has allowed the lake to persist for an unusually long time, preserving an exquisite lacustrine record since at least the mid-Holocene (Chen et al., 2012).

The climate of the Ilan area is humid and subtropical, influenced by both the East Asian summer and winter monsoons, with frequent typhoon activities in summer and autumn. The annual mean temperature is ~22.2°C and the average annual precipitation over past 50 years is roughly 2760 mm (Central Weather Bureau, www.cwb.gov.tw). The natural vegetation in the study area mainly consists of subtropical evergreen rainforest, although the dominating component of the vegetation is strongly influenced by topography and altitude (Su, 1984). The vegetation of the surrounding mountains consists of subtropical to warm temperate forests dominated by Lauro-Fagaceae elements common in the Castanopsis-Machilus Zone (Chen, 2000). Today, much of the plain is cultivated (mostly rice), or covered by herbaceous plants and cultivated trees (Lin et al., 2007).

The history of human settlement in northeastern Taiwan has been reconstructed from archaeological remains (Liu, 1995; Chen et al., 2007b) and historical documents (Yao, 1829; Lu, 1970; Knapp, 1976; Bai, 1991). The colonization history of the Ilan area can be traced back to Neolithic (~5000 yr BP) and Iron age (~1300 yr BP) settlements by aboriginal people of Taiwan (Liu, 1995; Chen et al., 2007b). Before the seventeenth century, Taiwan was primarily populated by aboriginal groups and only a limited number of Chinese migrants due to the isolation policy of the Chinese government (Lu, 1970). However, in the early seventeenth century Chinese settlers mainly from southeastern China started to migrate to Taiwan because of the civil war between the Ming and Qing (or Ching) dynasties on mainland China (Knapp, 1976). Moreover, during the two centuries after the Qing dynasty annexed Taiwan in AD 1683, Chinese settlers established agricultural villages from west to east across the island. Therefore, the settlement history of northeastern Taiwan began quite late compared to western and southern Taiwan due to its isolated geographical location (Lu, 1970; Bai, 1991).

The town of Ilan was first recorded as Kibanuran and occupied by an aboriginal people named Kavalan (meaning people living on the plain). Chinese settlers reached northern Ilan at ~AD 1768. After fighting with the aboriginal Kavalan people for several years, a settler named Sha Wu and his group finally began their agriculture practices near Toucheng (northern Ilan, Figure 1A) in AD 1796 (Lu, 1970). Along with the continuing development of Ilan Plain, Chinese settlers reached the area near Lake Meihua at ~AD 1874 and spread to the Suao area (southern Ilan) in the following decades (Lu, 1970; Bai, 1991).

The two short lacustrine cores MHL09-01 and MHL11-02 used for this study were collected on October 1^st 2009 and March 9^th 2011 from Lake Meihua (Figure 1B). To obtain high-quality, undisturbed cores, we used 9-cm diameter transparent acrylic tubes with sharp edges which were inserted directly into the lake sediments on the rubber boat. The tops of the tubes were sealed to maintain the air pressure, and the tubes were pulled up directly to get the lake sediments. Since the tubes were transparent, the interface between water and sediment can be clearly seen to make sure the sediments were well preserved. The recovery length for core MHL-09-01 was 76 cm, and 68 cm for core MHL-11-02. After collection, the cores were kept upright and transported to the laboratory for splitting, description, and subsampling. The archive-halves were used for Itrax-XRF core scanner analysis, and the working-halves were subsampled at a resolution of 0.5 to 1 cm for radionuclides, TOC, and TOC/TN analysis, and 1 to 5 cm for grain size analysis. In addition, the top 205 cm of the 28.63 m long core MHL-5A (Figure 1B), which was obtained using hydraulic piston coring in 2005, was used for palynological studies. The sampling intervals for the pollen study were every 10 cm with 1 cm slice thickness, and 2 ml of each slice were used for pollen analysis.

The age models for cores MHL-09-01 and MHL-11-02 were constructed by using excess ²¹⁰Pb (²¹⁰Pb_ex) and were further constrained by the subsurface maximum of ¹³⁷Cs and ^{239, 240}Pu (circa A.D. 1963), which are consistent with the history of nuclear fallout. In brief, ²¹⁰Pb (via ²¹⁰Po) and ^{239, 240}Pu in core MHL-09-01 were measured by α-spectrometry, and ²¹⁰Pb in core MHL-11-02 was measured by γ-spectrometry. ¹³⁷Cs in both cores was measured by γ-spectrometry. ²⁰⁹Po and ²⁴²Pu spikes obtained from ORNL (Oak Ridge National Laboratory), calibrated with NIST-certified SRM-4327 and SRM-996 standards, were used as the yield determinants and added before the digestion of the samples. The details of the radiochemical procedures can be found in Huh and Su (1999) and the references therein. After radiochemical procedures, the Po isotopes were plated onto a silver disc at 80–90°C for 1 h, and the Pu isotopes were electroplated onto a stainless steel disc. ²¹⁰Pb, ²¹⁴Pb, and ¹³⁷Cs in MHL-11-02 were measured by γ-spectrometry based on photon energies around 46.52 keV, 351.99 keV, and 661.62 keV, respectively. Three HPGe detectors were engaged in this study to count over a series of sectioned sediment samples, including one GEM-type (ORTEC GEM-150240) with 150% efficiency (relative to 3 × 3 NaI), one GMX-type (ORTEC GMX-120265) with 100–120% efficiency and one LoAX-type (ORTEC LoAX-70450) with 70% efficiency. The counting efficiencies of the three detectors were calibrated by IAEA reference materials 327 and 375 for sample weight at 100 g as a reference, coupled with an in-house secondary standard for various masses (from 20 to 250 g) to calibrate the effect of sample mass on the attenuation of γ-ray of various energies. Sedimentation accumulation rates were determined from depth profiles of ²¹⁰Pb_ex. The total ²¹⁰Pb and the fraction of the total ²¹⁰Pb that is supported by its precursor, ²²⁶Ra. Due to the very short half-lives of all intermediate nuclides between ²²⁶Ra and ²¹⁰Pb, the supported ²¹⁰Pb is generally in secular equilibrium with all its precursors from ²²⁶Ra to ²¹⁴Pb. The activity of ²¹⁰Pb_ex was obtained by subtracting the activity of ²¹⁴Pb from that of the measured (i.e., total) ²¹⁰Pb (²¹⁰Pb_ex = ²¹⁰Pb−²¹⁴Pb). In theory, the activity of ²¹⁰Pb_ex is the highest at the core top and decreases down-core because of radioactive decay. Under the assumption of a constant ²¹⁰Pb flux and a constant sedimentation rate (with the layer of Unit II excluded), the concentration of ²¹⁰Pb_ex downcore can be described by: (1)C=C0exp(-λt) where C is ²¹⁰Pb_ex at depth Z, C₀ is ²¹⁰Pb_ex at the core top (i.e., Z = 0), λ is the decay constant of ²¹⁰Pb (0.0311 yr⁻¹), and t is the post-depositional time. Given that t = Z/S, where S indicates sedimentation rate, previous equation can be transformed to (2)C=C0exp[(-λS)Z]

Therefore, we expect to see straight regression lines by plotting C vs. Z on semi-log plots, which have slopes equal to −λ/S. From the slopes, the sedimentation rate (S) can be determined. The details about the γ-spectrometry method, including the standard materials used to calibrate the detectors and QA/QC of the data, can be found in previous studies (Huh and Su, 1999; Su and Huh, 2002; Huh et al., 2011).

The age model for the uppermost sediments of core MHL-5A is based on the linear interpolation between the date of coring at top, and one AMS (Accelerator Mass Spectrometry) ¹⁴C analysis from wood fragments at 194 cm performed by the Rafter Radiocarbon Laboratory, Institute of Geological and Nuclear Sciences, New Zealand. The ¹⁴C date was then calibrated using CALIB 7.1.0. (http://calib.qub.ac.uk/calib/). All following ages are given as calibrated years AD.

Continuous downcore measurements of elemental variations were done at the Itrax-XRF Core Scanner Lab, Department of Geosciences, National Taiwan University (NTU). Archive halves of core MHL-09-01 and MHL-11-02 were analyzed using the 3 kW Mo target. The XRF measurements were analyzed at 30 kV, 44 mA, 2 mm resolution with 5 s exposure time for MHL-09-01, and 30 kV, 29 mA, 200 μm resolution with 15 s exposure time for MHL-11-02. The original XRF spectrums were processed by the Q-Spec software provided by Cox Analytical Systems to obtain element peak areas as counts. Beside the element counts, the ratio of incoherent to coherent scattering (inc/coh) may be used as an estimate of sedimentary organic contents in lacustrine sediments. In theory, the inc/coh scattering is higher for elements with a low atomic mass than for elements with higher atomic mass (Hodoroaba and Rackwitz, 2014), and therefore has been linked to the mean atomic number and can be largely related to the content of organic matter (Guyard et al., 2007; Rolland et al., 2008; Sáez et al., 2009; Brunschön et al., 2010). Therefore, the inc/coh can become an important reference for the strategy of further sampling, and thus to reduce the measure time and costs on organic experiments. Moreover, optical and radiographic images of the cores were also obtained by the same device.

Samples for total organic carbon (TOC) and total nitrogen (TN) were analyzed at the Department of Earth and Life Science, University of Taipei, following the procedures described in Yang et al. (2011b). In brief, each freeze-dried sample was pre-treated with 1 N HCl to remove carbonate, then washed with deionized water and dried in an oven at 60°C. The samples were then wrapped in a tin capsule and combusted using a ThermoQuest EA1110 elemental analyzer to measure the TOC and TN, and thus the atomic ratio of TOC/TN. The precision of the measurements for TOC and TN is ± 0.4 wt. % and ± 0.6 wt. %, respectively.

Samples for grain size analysis were analyzed at the Institute of Oceanography, National Taiwan University. 1 g of wet sediment was treated sequentially with 10 ml of 15% H₂O₂ and 7.5 ml of 10% HCl to remove organic materials and carbonates, and washed with deionized water. Prior to the analysis, 10 ml of 1% Na(PO₃)₆ was added to each sample followed by vigorous shaking. The samples were then ready for analysis using a Beckman Coulter LS-13 320 Laser diffraction particle size analyzer. Reproducibility of replicate analyses of sample and working standard were better than 95%.

For the palynological study, the sample preparation of core MHL-5A followed the classic procedures outlined by Moore et al. (1991), and slightly modified by Lin et al. (2007), involving treatments with hydrochloric acid, potassium hydroxide, sodium pyrophosphate, hydrofluoric acid and acetolysis. For each sample, more than 200 non-aquatic pollen grains were counted, except for samples with very low pollen counts. The taxonomic nomenclature in the pollen diagrams followed the published keys from Huang (1981), Chen and Wang (1999), and the references therein. Percentage calculations and construction of the diagrams were performed using the Tilia and TGView programs (provided by Dr. Eric Grimm, Illinois State Museum). In this study, we will only use the palynological data of the uppermost 205 cm from core MHL-5A.

Using sedimentological core descriptions, grain size analysis, and observations of radiographic images, five sedimentary units were defined and correlated between MHL-09-01 and MHL-11-02 (Figure 2, Table 1). From bottom to top, Unit V (58–76 cm in MHL-09-01 and 66–68 cm in MHL-11-02) consists of a laminated light gray-yellowish silt with gradual changes at the upper boundary. Unit IV (34–58 cm in MHL-09-01 and 34–66 cm in MHL-11-02) is a laminated light gray silt with gradual upper boundary. Unit III (17–34 cm in MHL-09-01 and 18–34 cm in MHL-11-02) is a soupy dark gray silt to very-fine-sand and has a sharp upper boundary especially in MHL-09-01. Unit II (10.5–17 cm in MHL-09-01 and 9–18 cm in MHL-11-02) is made up of a massive yellowish silt with gradual upper boundary, followed by Unit I (0–10.5 cm in MHL-09-01 and 0–9 cm in MHL-11-02), which is a homogenous dark gray silt. In this study, we will focus at Unit III to I. For more details on the lower units, please refer to Huang (2011).

Activities of all measured fallout nuclides show anomalously low values in Unit II for both cores (Figure 2). However, the ²¹⁰Pb_ex profiles of the segments above and below Unit II both show the expected exponential decreases with depths, allowing the sedimentation accumulation rates based on ²¹⁰Pb_ex to be estimated. Further chronological constraint comes from the profiles of ¹³⁷Cs which are characterized by a pronounced peak at the 18.0–18.5 cm interval in MHL-09-01 (also ^{239, 240}Pu peak) and 17.0–17.5 cm in MHL-11-02, reflecting the history of atmospheric nuclear weapons testing which showed a global fallout maximum in AD 1963 (Pennington et al., 1973).

In MHL-09-01, since the Unit II is covered by 10.5 cm of sediment at the top and commenced ~1.5 cm above the global fallout maximum (circa AD 1963), the age of the Unit II can be constrained by ²¹⁰Pb chronology (Figure 2). By least-square fitting of the ²¹⁰Pb_ex data above and below Unit II, we obtained ²¹⁰Pb-based sedimentation accumulation rates of 0.45 ± 0.12 cm yr⁻¹ (R² = 0.69, n = 10) and 0.19 ± 0.08 cm yr⁻¹ (R² = 0.98, n = 9), respectively. Thus, the 6.5 cm thick Unit II was deposited ~7 years after the fallout maximum and ~24 years before the core was collected. In other words, Unit II accumulated during AD 1970–1985, with a mean sedimentation accumulation rate of ~0.40 cm/yr. Also, the pattern of radionuclide profiles in MHL-11-02 is similar to those in MHL-09-01 (Figure 2). The ²¹⁰Pb-based sedimentation accumulation rates above and below Unit II are 0.34 ± 0.08 cm yr⁻¹ (R² = 0.61, n = 7) and 0.19 ± 0.06 cm yr⁻¹ (R² = 0.54, n = 8), while the sedimentation accumulation rate in Unit II is ~0.37 cm yr⁻¹. Therefore, the ages of Unit II in both cores are perfectly consistent with the construction period of San-Ching Gong Temple near the lake (see discussion).

The chronology in the upper 205 cm of MHL-5A is based on the linear interpolation between coring date (i.e., AD 2005) and 1 AMS ¹⁴C dating at 194 cm (wood fragments, the dating result is 400 ± 30 yr BP and the median calibrated age is 1476 in the 2σ range of AD 1437–1624, Table 2), resulting in a mean sedimentation rate of ~0.36 cm yr⁻¹. The general sedimentation rate indicated by these two age control points is supported by deeper radiocarbon dates in MHL-5A, and also similar to the sedimentation rates that were estimated for the two shorter cores in this study (Figure 2, Table 2), as well as by previous studies (Huang, 2011; Chen et al., 2012).

The TOC/TN can be used to identify the sources of organic materials in sediments as either autochthonous or allochthonous (Meyers, 1994; Lamb et al., 2006; Yang et al., 2011a, 2014). As documented by Meyers (1994) and Lamb et al. (2006), the TOC/TN is usually less than 9 for lacustrine algae (representing the autochthonous primary source) and higher than 12 for vascular plants (representing the allochthonous primary source), respectively. In the Lanyang River catchment, the TOC/TN of primary materials, such as bedrocks, river sediments and soils have been investigated by Kao and Liu (2000) (Figure 4), and also been used to identify the organic sources of lacustrine sediments as reported by Yang et al. (2014) and Yang et al. (2011a).

As suggested by the MHL-5A record in Huang (2011), the TOC and TOC/TN values in Unit IV of both MHL-09-01 and MHL-11-02 (Figure 4, blue circle and blue cross) can represent the long-term average conditions in Lake Meihua during the past millennium. The increasing trends of TOC/TN and TOC from Unit IV to Unit III of MHL-11-02 in Figure 4 (gray arrow) likely indicate an increasing input of carbon-rich terrestrial organic materials in both Unit III and Unit I. However, the bedrocks and river sediments cannot be the major components of these increasing signals because of their low TOC contents (~0.5%, Figure 4). Therefore, the soils and the plant debris (found in Unit III of MHL-11-02, Table 1) seem to be the main contributors of the increasing organic signals. Conversely, the abrupt drop in Unit II represents the strong dilution by minerogenic materials, indicated also by its low values of TOC, TOC/TN, and radionuclides.

However, the increasing trend of TOC/TN in MHL-09-01 is insignificant (Figures 3, 4), although the maximum values of TOC are actually two times higher than those in MHL-11-02. Such observation may be explained as the distance effects of the coring locations in the lake. The terrestrial organic material from the catchment may settle down before it reaches to the center of the lake, where MHL-09-01 was collected. Furthermore, since the TOC/TN in MHL-09-01 remains in the range of freshwater algae, the higher TOC in MHL-09-01 can simply be explained as the lacustrine algae blooming associated with the terrestrial inputs.

The yellowish Unit II in both cores is the most characteristic unit in the cores. However, the lower values of inc/coh, TOC, TOC/TN, radionuclides in Unit II are very different from the flood layers reported in previous study in Lake Meihua (Huang, 2011; also in the Unit V of this study). There are 8 layers with higher values of inc/coh, TOC, TOC/TN, and grain size in the last 1200 years, and they have been explained as the overbank flooding events and can be linked to the regional climate and earthquake signals (Huang, 2011). Therefore, the natural mechanism cannot explain the presents of Unit II.

The most conspicuous sign of human activities around Lake Meihua is the large temple complex called San-Chin-Gong Temple (Figure 6) which is sitting on the slope to the south of the lake (Figure 1B). As the headquarters of the Taoism Association in Taiwan, the main building of the temple was constructed over an area of 4 ha during AD 1970–1982, followed by the addition of several annexes in the ensuing years. Such a massive construction in a relatively small catchment must have had a great impact on local environment and the lake just beneath the hill.

The age models for the yellowish Unit II in both cores (Table 1, Figures 2, 3) are perfectly linked with the historical temple-building record (Figure 3). The radionuclide age at the upper boundary of Unit II in MHL-09-01 is AD 1985 (derived from the upper ²¹⁰Pb_ex sedimentation rate and the coring date), while the age at the lower boundary is AD 1970 (derived from the ¹³⁷Cs peak in AD 1963 and the lower ²¹⁰Pb_ex sedimentation rate). Thus, the age of Unit II perfectly match with the documented record of temple construction from AD 1970–1982.

The peaks of TOC, inc/coh and grain size at the boundary of Unit III and II also mark the initial stage of the temple building phase, especially in MHL-09-01. The intense deforestation and excavation for the foundation of the temple and surrounding road systems likely is responsible for these changes. Also, Unit II is characterized by relatively lower values of inc/coh, TOC, TOC/TN, radionuclides, and its yellowish color (Figures 2, 3). These properties reflect the dilution of organic matter and radionuclides content by increased minerogenic material inputs. The enhanced erosion of the exposed hillslope caused by land development activities introduced radionuclides, organic carbon depleted regolith, and/or even the materials that were used in the temple construction into the lake. These anthropogenic influences are further supported by the increased sediment rates in Unit II (0.40 cm yr⁻¹ in MHL-09-01 and 0.37 cm yr⁻¹ in MHL-11-02). Therefore, the presence of the yellowish Unit II represents the sedimentary evidence for the construction of the temple complex and surrounding road systems. These observations suggest that locally, human induced changes to the erosional pattern may temporally overshadow the natural signals.

After the construction event of the San-Chin-Gong Temple, the intensity of human activities has remained high in the study area. The construction of a dyke and a new sightseeing area around the lake in recent decades likely have caused the increase in sedimentation rates encountered in Unit I (Figure 2, 0.45 cm yr⁻¹ in MHL-09-01 and 0.34 cm yr⁻¹ in MHL-11-02). Also, the return to high levels of inc/coh, TOC and TOC/TN in Unit I (Figures 3, 4) suggest that runoff delivered more terrestrial organic materials into the lake related to the continuous farming and deforestation in the surrounding area.

In the older part of the record, human influence is less dramatic, but still clearly visible from both sedimentary parameters and pollen data. Up till the end of the eighteenth century, the area surrounding Lake Meihua experienced relatively little perturbations from human activities. It was not until AD 1796 that the Chinese settler Sha Wu and his group defeated the aboriginal Kavalan people and invaded the Toucheng area (northeastern Ilan, Figure 1A) to extend the frontiers of Chinese settlement and agricultural practices. After that, Chinese settlers started to develop the Ilan Plain, although they were constantly in conflict with the original aboriginal population while extending their territory further, reaching Suao at the southeastern tip of the Ilan Plain in the late nineteenth century (Yao, 1829; Lu, 1970).

The records from Lake Meihua in southern Ilan clearly reflect this history. This study reveals how the increasing trends of inc/coh, TOC, TOC/TN, and grain size in Unit III of both MHL cores (Figures 3, 4) record the agronomic activities of Chinese settlers starting from the late nineteenth century. Historical documents suggest that a Chinese settler named Hui-Huang Chen and his group of people started the cultivation works to the south of Lanyang River in AD 1874 (Bai, 1991), at a site close to Lake Meihua. The deforestation, land clearance and following agricultural practices by Chinese settlers likely caused intensified remobilization of terrestrial material, thereby not only increasing the input of TOC and coarser materials into the lake, but also causing algae booming due to the input of nutrients. These changes in input are also reflected by an increase in plant debris of MHL-11-02, and the less dense nature of the sediments shown in the radiographic images of both cores (Table 1, Figure 2).

The change in land use is further supported by the palynological data from core MHL-5A (Figure 5, PZ1). The dramatic drop in tree and shrub pollen can be attributed to deforestation, while the dominance of herbs and pioneer species, such as Alnus, Mallotus, and Trema since around AD 1860 (Figure 5, PZ1) likely is the result of anthropogenic vegetation changes due to land clearance, and can therefore be linked to the increasing organic content in the Unit III (Figure 3). In the present age model of core MHL-5A, the age for the lower boundary of pollen zone PZ1 is situated at about AD 1860 (Figure 5). This is just one decade earlier than the change in agriculture activities indicated by the historical record (Bai, 1991) and the radionuclides ages in the beginning of Unit III in both short cores. However, the low resolution sampling for radiocarbon dating in core MHL-5A makes the exact age of the shift in pollen assemblages uncertain. The sedimentation rates obtained by ²¹⁰Pb_ex together with the radionuclides fall out maximum in AD 1963 obtained by ¹³⁷Cs and ^{239, 240}Pu in MHL-09-01 and MHL-11-02 suggested that the lower boundary of Unit III can be dated to the 1880s (Figure 3). Thus, the evidence of anthropogenic impacts in the lacustrine cores from Lake Meihua may certainly be linked to the agricultural practices by Chinese settlers since AD ~1874, as recorded in historical documents (Bai, 1991).

In contrast to Lake Meihua, where anthropogenic influence can only be clearly detected for the last few 100 years or so, palynological records from Lake Dahu in northern Ilan show a human influence at least since around AD 1400. This suggests that on the northern Ilan Plain, intensive land clearance took place 200 years prior to the arrival of the Chinese settlers (Yang et al., 2014). Consequently, the great drop in arboreal pollen concentration around AD 1400 in Lake Dahu can be attributed to deforestation by aboriginal people to clear land for agriculture. According to the archaeological studies on Ilan Plain (Liu, 1995), the almost 500 years earlier development history in Lake Dahu compared to Lake Meihua can be attributed to the distribution of aboriginal people on Ilan Plain and the later arrival of Chinese settlers in southern Ilan.

This study suggests that while modest land clearance may result in an increase in TOC due to enhanced erosion of the topsoil, a massive intervention, however, such as the temple construction may actually result in a drop in TOC due to dilution of the organic input by massive influx of lithogenic material from the exposed regolith. Where the local end-members of the organic sources are known, TOC/TN can be used to identify the sources and possible transport mechanisms of sediments.

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.