Female mate preferences for male secondary sexual traits have been intensively studied across a diversity of taxonomic groups (Rosenthal, 2017). Females often prefer males with costly, condition-dependent secondary sexual traits such as conspicuous courtship displays, complex acoustic signals or bright coloration, as these traits function as honest indicators of male quality (Kokko et al., 2003; Rosenthal, 2017). By choosing to mate with high quality males that possess more elaborate traits, females can gain direct “material” benefits (such as resources or paternal care) and/or indirect “genetic” benefits (such as good or compatible genes for offspring) (Neff and Pitcher, 2005; Kuijper et al., 2012). Compared to the large body of evidence investigating female mate preferences at the population level, relatively few studies have considered variation in female preferences for male traits (Jennions and Petrie, 1997; Rosenthal, 2017). In particular, we still know little about whether females within populations vary in their preferences (among-individual variation) or whether individual females are consistent with their preferences over time (within-individual variation) (Bell et al., 2009; Zandberg et al., 2017, 2020). However, empirical research exploring such variation is gaining momentum, with evidence suggesting that individual variation in mate preference may be more common than currently realized (Forstmeier and Birkhead, 2004; Cummings and Mollaghan, 2006; Bell et al., 2009; Fowler-Finn and Rodríguez, 2013; Ah-King and Gowaty, 2016; Zandberg et al., 2017; Aich et al., 2020).

Quantifying the repeatability of mate choice behavior has been identified as an important first step toward understanding how mate preferences vary within populations (Widemo and Sæther, 1999; Brooks and Endler, 2001; Dougherty, 2020). Repeatability is defined as the amount of behavioral variation due to differences between individuals, and is calculated by dividing the among-individual variance by the total phenotypic variance (the sum of among-and within-individual variance) (Bell et al., 2009; Dingemanse and Dochtermann, 2013). Thus, when individuals differ in their average behavior (i.e., leading to high among-individual variance in the sample), and also behave consistently over time (i.e., display low levels of within-individual variance) repeatability is high (Bell et al., 2009). In the context of female mate choice, if females differ in their average choices over time (high among-individual variation), and individual female choices are stable over time (low within-individual variation), repeatability estimates will be high (Bell et al., 2009). Conversely, if individual females are random in their mate choices or exhibit plasticity in mate preferences (high within-individual variation), or, if females are mostly unanimous and consistent with their mate choices over time (low among- and within- individual variation), repeatability estimates will be low (Rosenthal, 2017). Examining the repeatability of mate choice can therefore offer important insights into the evolution of mate preferences, and provide empiricists with information relevant to understanding the particular benefits gained from mate choice decisions (Jennions and Petrie, 1997). Crucially, repeatable variation in mate choice can have major evolutionary consequences as it can directly influence the intensity and direction of sexual selection, which may have indirect flow on effects for population viability and fitness (Jennions and Petrie, 1997; Brooks and Endler, 2001; Cally et al., 2019).

From a conservation perspective, there is growing recognition that knowledge of mate choice, as well as individual variation in mate choice, can facilitate successful conservation breeding programs (CBPs) (Wielebnowski, 1998; Asa et al., 2011; Chargé et al., 2014). CBPs aim to establish viable captive assurance population's ex-situ and provide large numbers of genetically diverse individuals for reintroduction in-situ (Pritchard et al., 2012). Unfortunately, breeding success in captivity can be highly variable, and reproductive failures often occur (Wielebnowski, 1998; Conway, 2011). One reason for this may be that natural mate choice behavior is rarely permitted; either due to a lack of information on the reproductive behavior of a target species, and/or, mate choice is restricted in favor of genetic targets (Asa et al., 2011; Martin-Wintle et al., 2019). Recent work has demonstrated that integrating mate choice into captive breeding protocols can significantly increase behavioral compatibility between mates, resulting in higher mating and reproductive success, and elevated offspring survival post-release (Petrie, 1994; Martin-Wintle et al., 2015; Hartnett et al., 2018; Parrott et al., 2019). Thus, research investigating patterns of female mate choice (including individual variation) in threatened species has real potential to improve captive breeding outcomes. Understanding the mechanisms of female mate choice, and whether females differ in their mate preferences over time, would provide conservation managers with the necessary information to predict and manipulate mate choice in captive environments and anticipate reproductive outcomes (Asa et al., 2011). This type of behavioral research will undoubtedly be an important tool to help improve the captive breeding, genetic management and post-release success of many critically endangered species.

Amphibians are one taxonomic group that stand to benefit substantially from integrative, behavior-based conservation approaches (Kelleher et al., 2018). Amphibians are currently the most threatened vertebrate group (an estimated 41% of species are threatened with extinction), and ex-situ CBPs have been established worldwide to aid in the recovery of critically endangered amphibian species (IUCN, 2020). However, breeding success in amphibian CBPs is often poor (Soorae, 2016), most likely owing to the complexity and diversity of amphibian reproductive ecologies (Haddad and Prado, 2005). Importantly, anuran amphibians have been a model system for the study of sexual selection for over half a century (Wells, 2007), so empiricists have a firm understanding of the mechanisms of female mate choice in this taxonomic group. Surprisingly, however, this vast body of knowledge is seldom integrated into amphibian conservation efforts (Walls and Gabor, 2019).

In nearly all anuran amphibian species, reproduction relies heavily on acoustic communication (Wells, 2007). In most species, males actively advertise to females by calling, and acoustic signals play a crucial role in both mate attraction and mate selection (Gerhardt and Huber, 2002). Females have been shown to discriminate amongst potential mates based on a range of different call characteristics, such as call rate (Laird et al., 2016), call duration (Welch et al., 1998), and call effort (Ward et al., 2013). Dominant call frequency (or call peak frequency) is one component of a male's advertisement call that usually varies among males and is often negatively correlated with body size (larger males have a lower dominant call frequency) (Gerhardt and Huber, 2002; Wells, 2007). Female anurans have been shown to display preferences for lower than average call frequencies, and selection on call frequency is often reported to be weakly directional or stabilizing (Wells, 2007). Females may use male call frequency as a cue to assess male body size or age (Vargas-Salinas et al., 2014), and by discriminating among males based on frequency potentially gain direct or indirect benefits, such as higher fertilization success (Robertson, 1990), or the acquisition of good genes that improve offspring performance and survival (Rausch et al., 2014).

Compared to the extensive research investigating female preferences for male call characteristics in anurans at the population level, among-individual variation in preferences, or the repeatability of such preferences, remain largely unknown. To date, the small number of studies that have examined the repeatability of mate preferences in anurans have yielded mixed results (Gerhardt and Huber, 2002). Some studies have reported that females display repeatable, among-individual variation in preferences for male call characteristics such as call duration (Gerhardt et al., 2000) and call frequency (Jennions et al., 1995; Howard and Young, 1998). Conversely, studies in other species, such as midwife toads (Alytes muletensis), and tungara frogs (Physalaemus pustulosus) have shown that there can be substantial within-individual variation in mate preferences (attributable to random mating and plasticity), resulting in low repeatability estimates (Kime et al., 1998; Lea et al., 2000). Clearly, there is considerable inter- and intra- specific variation in mating preferences in anurans, and further assessment of its repeatability is needed. For threatened species, this knowledge can potentially be utilized by conservation managers to increase the chance of individuals successfully breeding in captivity, or, facilitate social manipulations that maximize reproductive output whilst simultaneously enhancing the genetic management of captive populations.

The southern corroboree frog, Pseudophryne corroboree, is a terrestrial breeding anuran that is endemic to Kosciuszko National Park in south eastern New South Wales, Australia. Since the 1980s P. corroboree has suffered extreme population declines, predominantly due to lethal effects caused by the introduced amphibian chytrid fungus (Batrachochytrium dendrobatidis) (Hunter et al., 2010). In response to such alarming population declines, a multi-institutional CBP was established in 2003 (OEH NSW, 2012). While P. corroboree can reproduce successfully in captivity, mating success is restricted to a small proportion of advertising males (30–50%) (McFadden et al., 2013). At present, we know very little about patterns of mate choice (McFadden et al., 2013). In nature, we know that P. corroboree males construct shallow terrestrial nests and call to attract potential mates (Osborne, 1991). In captivity, female mate choice has been observed in captive breeding enclosures, where certain males are more successful than others at attracting females and gaining matings, and some females will refrain from mating if they do not encounter a suitable partner (McFadden et al., 2013). It is currently unknown which male characteristics are attractive to female P. corroboree, and what cues females utilize to assess mate quality. Identifying female preferences for male phenotypic cues (as well as whether females vary in their mate preferences), may help maintain viable captive populations of P. corroboree by providing conservation managers with the knowledge needed to conduct captive breeding manipulations that may increase the reproductive success of genetically valuable individuals. The aim of the present study was to gain preliminary insights into patterns of female mate choice in captive southern corroboree frogs. The specific aims were to determine whether females: (1) exhibit preferences for low, average or high frequency male advertisement calls, and (2) vary in their preferences for male call frequency over repeated trials. Given that past work has shown that female body size can influence variation in mate choice in amphibians (Jennions et al., 1995; Neelon et al., 2019), we also considered whether female body size influenced female mate preferences. We examined female mate preferences by conducting repeated phonotaxis choice trials, where females were given the opportunity to choose between low, average or high frequency male calls, with a subset of females tested three times to determine preference repeatability.

In all trials (first trial and repeated trials), all females left the no choice zone and exhibited positive phonotactic behavior to the acoustic stimuli broadcast in the experimental arena. Females crawled freely toward the speakers, with the majority of females remaining within 1 cm of one of the speakers, or directly touching it, indicating that they were responsive to the calls. During the first trial, a subset of females visited up to five speakers, though most females (62%) visited a single speaker. In the first trial, females spent significantly less time on average in the low frequency call zone than expected by chance (t-test: t = −3.44, df = 39, p = < 0.01; Figure 3). The mean proportion of time spent in the medium frequency call zone did not deviate from chance (t-test: t = −2.07, df = 39, p = 0.14; Figure 3), nor did the mean time spent in the high frequency call zone (t-test: t = −0.1, df = 39, p = 1; Figure 3). Based on overall preference (the zone females spent the maximum amount of time in), there was no evidence that females preferred low, medium or high male call frequencies (binomial test: n = 40, p_low = 0.95, p_medium = 1, p_high = 0.39).

Compared to the overwhelming number of studies investigating population-level mate preferences, research investigating individual variation in female mate preferences has remained limited. This is surprising as variation in mate preferences at both the among- and within-individual level can have major implications for the development of sexual selection theory, and threatened species conservation. Here, we investigated whether female P. corroboree exhibited preferences for male calls that differed in frequency, and whether individual females varied in their preferences over three repeated trials. For the first trial, our results showed that females did not exhibit a population-level preference for call frequency, yet, the mean proportion of time females spent in the low frequency call zone was lower than expected by chance. We found evidence that female mate preferences were significantly affected by trial number, whereby time spent in the low frequency call zone increased over repeated trials, and low frequency calls were significantly preferred in the last trial. Subsequently, there was no significant repeatability in preference based on any of the measures used. Overall, these findings suggest that female P. corroboree mate preferences for call frequency are dynamic, and highlight that individual females' may alter their mate preferences within a single breeding season, providing evidence for plasticity in female mate preferences.

Our finding that P. corroboree females did not display a population-level mate preference for call frequency during the first trial was surprising because there is a large body of empirical evidence that female anurans prefer average to low call frequencies, a trait known to correlate with male body size and reliably signal male quality (Gerhardt and Huber, 2002; Wells, 2007). Previous work in anurans has demonstrated that phenotypic variation in female preferences for male call frequency can often be attributed to size-assortative mating. For example, in the African painted reed frog (Hyperolius marmoratus), females differ in their preferences for male call frequency due to size-dependent discriminatory abilities, most likely related to differences in the frequency tuning of the basilar papilla in female ears (Jennions et al., 1995). Similarly, in the smooth toadlet, (Uperoleia laevigata), females prefer the call frequency of males that are ~70% of their own body weight, as this allows for proper alignment of the cloaca during gamete release, which facilitates successful fertilization (Robertson, 1990). However, these reasons are unlikely to explain the lack of population-level preference observed in the first trial because we found no evidence that female body size affected the likelihood of choosing any particular call frequency. An alternative reason why we failed to detect a population level preference for call frequency during the first trial may relate to the way we produced our call stimuli. As we manipulated a natural exemplar call taken from an individual male, we can't be certain that we would have found similar results if we had manipulated the calls of a different male. Arguably, call frequency might only be important for phonotaxis when presented in combination with certain call trait values that are difficult to characterize. To address this issue, future preference tests with P. corroboree should make replicated sets of manipulated trait values using different male calls for each trial (e.g., Oliva et al., 2018). Until this work has been conducted, explanations for a lack of a population-level preference in the first trial will remain speculative. Unexpectedly, we found that females in the first trial spent less time in the low frequency zone than predicted by chance. This result implies that females may have been initially avoiding males with low frequency calls, or, spending less time assessing them. While the reason for this result remains unclear, one possibility is that higher frequency calls were easier to detect in a noisy environment, as has been previously shown in both anurans (Bee, 2008; Parris et al., 2009) and birds (Lohr et al., 2003; Cardoso and Atwell, 2011).

Critically, however, we found that the time females spent in the low frequency call zone significantly differed among subsequent trials, and, by the third trial, females significantly preferred low frequency calls. These findings suggest that females may have initially been selecting males at random (based on overall preference), but as the breeding season progressed, females altered their preferences, resulting in a directional population-level preference for low frequency advertisement calls. This finding is particularly noteworthy as it is in line with a growing body of evidence that individual female mate preferences can exhibit phenotypic plasticity over sequential trials (Gabor and Halliday, 1997; Qvarnström et al., 2000; Wacker et al., 2016). This draws added attention to the potential for female mate preferences to display significant temporal variation within a single reproductive season.

One explanation for the change in female mate preference over sequential trials may be that females become choosier over time, as previously reported in other taxa (Forsgren, 1997; Gabor and Halliday, 1997; Uetz and Norton, 2007). Female choosiness (commitment to finding and assessing males) (Jennions and Petrie, 1997; Brooks and Endler, 2001) can vary due to a multitude of factors, including female age (Atwell and Wagner, 2014), physiological condition (Judge et al., 2014) and the risk of predation (Atwell and Wagner, 2015). A female's current reproductive state can also heavily influence an individual's degree of choosiness within a short time period (Lynch et al., 2005). Empirical evidence in numerous taxonomic groups, including anurans, has demonstrated that as individual females transition through different stages within their reproductive cycle, fluctuations in circulating hormone levels can mediate changes to preference thresholds for male signals (permissiveness), and alter a female's degree of choosiness, resulting in plasticity in mate choice (Lynch et al., 2005, 2006). For instance, in female midwife toads (A. muletensis), reproductive state has been shown to influence mating preferences, whereby ovulating females are highly receptive and more discriminatory, compared to gravid or mated females, who were less receptive and less choosy (Lea et al., 2000). Plasticity in mate choice attributable to changes in reproductive state has also been reported in tungara frogs (P. pustulosus) where females are initially selective, but as they approach the end of their reproductive cycle become more permissive and less choosy as they are constrained by a dwindling window of time in which they must oviposit their eggs (Lynch et al., 2005; Baugh and Ryan, 2009).

In our study, it is possible that during the first trial P. corroboree females were not in the optimal physiological state to mate (despite visually appearing gravid) and were thus receptive to male calls yet behaved relatively indiscriminately (based on overall population-level preference). As the females used in this study had never been previously exposed to male acoustic signals, it is possible that auditory stimulation from male calls during the first trial triggered a cascade of hormonal changes that altered receptivity (Wilczynski and Lynch, 2011). Indeed, there is strong evidence that sexual arousal in female amphibians is heavily reliant on hearing male acoustic signals, and that male calls alter female reproductive state by modulating changes in estrogen levels, which eventually triggers ovulation (Wilczynski and Lynch, 2011). Thus, after the first trial, hormonally primed P. corroboree females may have then become more discriminatory (as their reproductive state changed), resulting in a significant population-level preference for low call frequency by the last trial. Overall, there is considerable potential for female P. corroboree to vary in their choosiness within a single reproductive cycle. Pseudophryne corroboree have a breeding season that extends over multiple weeks (Osborne, 1991), so females have the time and opportunity to invest in mate choice (Wells, 2007). In nature, females are likely to visit the nests of multiple males prior to mating, as has been observed in other Pseudophryne species (Byrne and Keogh, 2007). This is also corroborated by observations of P. corroboree in captivity, where females can spend days to weeks assessing several males before selecting a mate (S. Kelleher, unpublished data). Although the exact time frame from ovulation to oviposition is currently unknown, it is likely that female P. corroboree do not ovulate until after they have entered a breeding site and are engaged in amplexus with a chosen male, as observed in three closely related sister species with similar reproductive ecologies, Pseudophryne bibronii, Pseudophryne dendyi and Pseudophryne semimarmorata (Woodruff, 1976). Additionally, there is some evidence for sequential polyandry in P. corroboree (Pengilley, 1973; McFadden et al., 2013), so females may be able to release a partial clutch of eggs, whilst retaining the remaining eggs for subsequent matings, as has previously been reported in other Pseudophryne species (Woodruff, 1976; Byrne and Keogh, 2009). Therefore, female P. corroboree may not be subjected to the same time constraints as reported in other anuran species that decrease their choosiness during ovulation (such as explosive or seasonal breeders that typically ovulate before entering a breeding site and are then committed to oviposition within hours or days to avoid a loss of egg fertilization capacity) (Lea et al., 2000; Lynch et al., 2006; Baugh and Ryan, 2009). Future studies should endeavor to test these ideas experimentally. This could be achieved by conducting manipulative experiments where female reproductive state is controlled by administering hormones and female phonotactic responses are measured (for example see Lynch et al., 2006).

An alternative explanation is that female P. corroboree mate preferences may change over sequential trials due to increased experience in evaluating male signals (Wagner et al., 2001; Caro et al., 2010). It is well-established that female mate preferences and degree of choosiness can be highly dependent on a female's prior social experience and past exposure to male signals (also referred to as experience-mediated plasticity) (Fowler-Finn and Rodríguez, 2012a,b). Numerous empirical studies have reported that experience-mediated plasticity can result in acquired, weakened or even reversed mate preferences due to effects on preference thresholds and choosiness (Walling et al., 2008; Fowler-Finn and Rodríguez, 2012a). For example, in field crickets (Teleogryllus oceanicus) naïve females that had never been previously exposed to male calls were receptive, but less discriminate in mate choice compared to females that had prior experience with acoustic signals, which were highly discriminate (Bailey and Zuk, 2008). Similarly, in wolf spiders, inexperienced females showed no directional mate preferences for ornamented males, but females who had previously been exposed to a variety of male phenotypes developed a preference for ornamented males (Hebets and Vink, 2007). Taken together, these studies emphasize that mate preferences can differ dramatically between experienced and inexperienced females, and that prior experience may actually be required before females can develop a preference for particular males (Bailey and Zuk, 2008). As the females used in the present study were virgins, with no prior experience with male acoustic signals, it is highly plausible that preferences changed over repeated trials due to effects associated with experience-mediated plasticity. As experience-mediated plasticity can also result in reversed preferences (Walling et al., 2008), this line of reasoning may also explain why females initially appeared to avoid low call frequencies, but then preferred these calls in the last trial. To determine the effect of previous experience in P. corroboree, future work should consider repeating this experiment with females previously exposed to male signals. Experience-mediated effects may be attributed to increased mate sampling that occurs over sequential trials. Theoretical models predict that female choosiness increases as females' sample and assess a greater number of males, resulting in stronger directional selection on male traits (Muniz and Machado, 2018). For example, if females adopt a “best of N males” sampling tactic, where they assess a number of potential mates (N) and choose between them, sexual selection is predicted to be most intense when females sample above a critical threshold of males, and, when females can only sample a small number of males, preferences are harder to detect (Benton and Evans, 1998; Muniz and Machado, 2018). In P. corroboree, females may have become choosier in subsequent trials because there were more opportunities for mate sampling, resulting in a population-level preference for low frequency male calls in the final trial. It is also important to note that when females sample mates simultaneously, and over various time points (as is generally the case in frog choruses), they may make comparative mate choices based on the relative attractiveness of the available mates to each other, irrespective of their absolute preference (Lea and Ryan, 2015; Zandberg et al., 2020). This comparative evaluation of potential mates may facilitate temporal plasticity in mate preferences, depending on the availability and relative attractiveness of the potential partners that are sampled (Lea and Ryan, 2015; Neelon and Höbel, 2017; Zandberg et al., 2020). Consideration of relative mate preferences may be of particular importance to CBPs as the specific males available to be sampled in a captive setting could influence female mate choice decisions (Neelon and Höbel, 2017). Additionally, as for many mate preference studies based on auditory cues alone, females were unable to find a male and subsequently mate (i.e., they were not rewarded by assessing and finding a male). Thus, it is possible that patterns of mate preference may differ when females can physically interact with potential mates, and decide to mate or not.

We found no significant repeatability (consistent, individual differences) in female mate preferences, based on any of the measures used. Females were generally unanimous in their mate preferences by the last trial (low among-individual variation) and females were inconsistent in their preferences across repeated trials (high within-individual variation), resulting in low repeatability estimates. Despite providing no evidence for repeatability, this finding is particularly intriguing as it draws attention to the potential for individual P. corroboree females to be receptive to a broader range of male phenotypes, and vary substantially in their mate preferences within a relatively short time period. In line with this finding, a growing number of studies in frogs (Lea et al., 2000; Lynch et al., 2005, 2006), birds (Qvarnström et al., 2000), fish (Tinghitella et al., 2013; Wacker et al., 2016) and invertebrates (Filice and Long, 2017; Kelly, 2018) have demonstrated that there can be substantial within-individual variation in female mate choice, attributed to phenotypic plasticity (Ah-King and Gowaty, 2016; Rosenthal, 2017). This growing body of evidence suggests that mate choice plasticity might be highly adaptive, as inflexible mate choice behavior has the potential to be costly in dynamic environments where the quantity and quality of potential mates can vary markedly (Qvarnström et al., 2000). In amphibians in particular, breeding is inextricably linked to climatic conditions which are often highly variable (Wells, 2007), so females may need to exhibit reversible plasticity in their mating decisions to ensure mating success under various conditions, such as when preferred mates are scarce (Fowler-Finn and Rodríguez, 2012b; Tinghitella et al., 2013). Subsequently, we expect that further empirical research investigating individual variation in mate preferences in amphibians will reveal that plasticity in mate preferences and subsequent choice is more widespread than currently realized, which contradicts the widely held view that female mate choice behavior in amphibians is often stereotyped and uniform (Baugh and Ryan, 2009).

Overall, the information gained from this study provides preliminary insights into the reproductive behavior of one of Australia's most critically endangered vertebrate species. Perhaps most importantly, our study shows that female P. corroboree exhibit strong behavioral responses to synthesized acoustic stimuli, and are highly receptive to the calls presented to them (even without the presence of a live male). This strong phonotactic behavior indicates that there is good potential to manipulate female mate choice in captivity to achieve reproductive outcomes that benefit conservation breeding (Fisher et al., 2003). To date, this type of work has focused on mammals, such as captive harvest mice, pygmy loris' and striped face dunnarts. In these species, male olfactory cues (scent markings) have been used to manipulate female preferences and determine optimal pairings in captivity (Fisher et al., 2003; Roberts and Gosling, 2004; Parrott et al., 2019). Whether similar approaches can be taken with anurans is an exciting avenue for future research. Interestingly, our results suggest that female P. corroboree exhibit a population-level preference for low frequency male advertisement calls after repeated exposure. This finding indicates that male call frequency may play a role in female mate choice decisions in P. corroboree and provides conservation managers with the first information on female mate preferences (and predictors of male attractiveness) in P. corroboree. However, our results simultaneously highlight the possibility for there to be within-individual variation in female preferences throughout a single breeding season. These findings emphasize that a single snap shot measurement of mate preference might not provide conservation managers with accurate information to be able to predict and manipulate mate preferences in captive populations. Conservation managers may need to consider the potential for mate preferences to exhibit plasticity in order to successfully incorporate mate choice into CBP management. For instance, if individual preferences vary throughout the breeding season, it might be useful to rotate the males presented to females at different critical time points in a breeding cycle. For example, if females are less choosy at the start of their reproductive cycle, they may be more permissive in their mate choices. As choosiness increases, females may need to be presented with a different set of potential mates. Similarly, if mate preferences are altered by experience-mediated plasticity, younger, inexperienced females may initially be less selective and more willing to mate with less attractive but genetically valuable males, compared to older, experienced females. If this is the case, there may be value in keeping a proportion of captive females unexposed to male signals, as this could allow conservation managers to conduct breeding manipulations that may increase the reproductive success of genetically valuable individuals, and subsequently, increase the genetic variation (and possible adaptive potential) of captive populations (Asa et al., 2011). Overall, further work is needed in P. corroboree to build on these preliminary insights and more comprehensively understand individual variation in mate choice before incorporating this knowledge into captive management. Nevertheless, our findings provide a platform for continued research into patterns and mechanisms of mate choice, which will advance our knowledge of P. corroboree reproductive ecology and inform management practices.

More broadly, our findings add to the growing body of evidence in amphibians and other vertebrates that individual female mate preferences can vary within relatively short time scales, such as a single reproductive cycle. Our study advances our understanding of female reproductive behavior by emphasizing the importance of considering individual variation when investigating mate preferences. Future mate choice studies should endeavor to test individuals repeatedly, as population-level patterns may mask important sources of variation (Dougherty, 2020). Such individual variation is not only important for advancing our understanding of the proximate and ultimate control of female mate choice behavior, but is also likely to have major implications for the application of mate choice to conservation breeding programs.

The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author.

This study was reviewed and approved by the University of Wollongong Animal Ethics Committee (Protocol Number AE17/14).

SK, PB, and AS conceived the study. SK and PB ran the experiments. SK analyzed all behavioral data and wrote the manuscript with input from all authors. ND advised on the statistical analyses. AH conducted the statistical analyses. All authors contributed to the article and approved the submitted version.

The study was funded by the Australian Research Council (Linkage Grant LP170100351) awarded to PB and AS and the University of Wollongong SMAH Small Project Grant (262 27 0976) awarded to PB and AS. This study was also supported by the Holsworth Research Endowment – Equity Trustees Charitable Foundation and the Ecological Society of Australia, and a Frog and Tadpole Study Group of New South Wales student grant awarded to SK. This work was conducted while SK was in receipt of an Australian Government Research Training Program (RTP) Scholarship. AH was supported by the German Science Foundation (DFG, HE 8857/1-1).

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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