AUTHOR=Kuriyakose Delna , M. Gowsalya 
  
TITLE=Explainable AI uncovers novel EEG microstate candidate neurophysiological markers for autism spectrum disorder
  
JOURNAL=Frontiers in Computational Neuroscience
  
VOLUME=Volume 20 - 2026
  
YEAR=2026
  
URL=https://www.frontiersin.org/journals/computational-neuroscience/articles/10.3389/fncom.2026.1763727
  
DOI=10.3389/fncom.2026.1763727
  
ISSN=1662-5188
  
ABSTRACT=BackgroundAutism spectrum disorder (ASD) is a complex neurodevelopmental condition characterized by atypical brain connectivity and impaired cognitive flexibility. Electroencephalography (EEG) based microstate analysis provides insight into the rapid temporal dynamics of brain networks, offering potential biomarkers for ASD.ObjectiveThis study proposes an interpretable classification framework for ASD diagnosis using multidomain microstate-informed features derived from EEG, integrating temporal, spectral, complexity-based, and higher-order metrics to comprehensively characterize brain dynamics.MethodsResting state EEG data from 56 participants (28 with ASD and 28 neurotypical controls; age range: 18–68 years) from the publicly available Sheffield dataset were preprocessed and segmented into microstates using a data-driven clustering approach. From these microstate sequences, we extracted a rich set of features across four domains: (i) temporal, (ii) spectral, (iii) temporal complexity, and (iv) higher-order metrics. Multiple classifiers were evaluated using 10-fold cross-validation, with hyperparameter tuning via a randomized search.ResultsAmong all classifiers, XGBoost achieved the highest performance, with an accuracy of 80.87% when utilizing the complete multidomain feature set, significantly outperforming single domain models. Explainable AI analysis using SHapley Additive exPlanations (SHAP) identified the top 20 discriminative features, including fractional occupancy derivative for microstate 3, delta-band power in states 1 and 3, and mean inter-transition interval. Retraining XGBoost on these SHAP-selected features yielded 80.34% accuracy, confirming their robustness as potential biomarkers. Statistical validation via Mann–Whitney U-tests and effect size measures further established their significance.ConclusionThe findings from the study demonstrated that microstate-informed features capturing temporal instability, transition unpredictability, and spectral alterations serve as clinically relevant and interpretable candidate neurophysiological markers of ASD, offering translational potential for objective diagnosis, treatment monitoring, and personalized interventions.