AUTHOR=Zhou Xiao , Zhang Lei , Lao Yuwen , Zhou Bin , Zhu Zhongquan 

TITLE=Developing a dichlorosalicylaldehyde-derived fluorescent probe for monitoring glutathione in a cellular pulmonary ventilation model

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 13 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2025.1698116

DOI=10.3389/fchem.2025.1698116

ISSN=2296-2646

ABSTRACT=Herein, a fluorescent probe, DSNBD-GSH, was developed from dichlorosalicylaldehyde to monitor glutathione (GSH) in a cellular pulmonary ventilation model. Both the normoxia and hypoxia conditions were evaluated. DSNBD-GSH responded to GSH in a dosage-dependent manner with a fluorescence signal at 478 nm under the excitation of 365 nm. The solution tests indicated that DSNBD-GSH had relatively high sensitivity for GSH, and the photophysical properties were stable in various conditions. Other advantages included rapid response, high selectivity, and low cytotoxicity. Most significantly, monitoring the GSH level in both hypoxia and recovery status allows for visualization of ventilation-related redox changes. This work highlights a referenceable case for improving the pulmonary ventilation approach during the perioperative period.