AUTHOR=Wang Long , Chen Weihua , Wan Yuqi , He Aimin , Liu Jiayin , Hu Xuemin TITLE=Extraction of tobacco extract from discarded tobacco leaves for cigarette yarns dyeing and neophytadiene separation JOURNAL=Frontiers in Chemistry VOLUME=Volume 13 - 2025 YEAR=2025 URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2025.1682505 DOI=10.3389/fchem.2025.1682505 ISSN=2296-2646 ABSTRACT=A continuous process integrating extracting valuable compounds from waste tobacco leaves for cigarette yarns dyeing and neophytadiene separation was developed, enabling sequential production of tobacco crude extract, colored yarns, tobacco-derived absolute, and neophytadiene. Systematic comparison of residues from four regions revealed that Lanxiong-derived biomass demonstrated the highest extract yield (25%), providing optimal raw material for scaled neophytadiene production. GC-MS and NMR analyses confirmed >98% purity and structural integrity of purified neophytadiene. When the crude extract was applied directly to cotton yarns dyeing, the dyed yarn with 1.5 wt% tobacco crude extract solution from Zunyi achieved a K/S value of 2.013 and exhibited a yellow-brown hue shift that met visual identification requirements for cigarette yarns. The dyed yarns retained over 90% tensile strength of untreated controls, while elongation and unevenness indices showed no statistically significant alterations. This integrated approach establishes a green and sustainable technological route for valorizing tobacco waste within circular economy frameworks.