A series of 2,5-substituted furan derivatives 1a–o were synthesized, as illustrated in Schemes 1, 2. In Scheme 1, the commercially available 5-bromofurfural was treated with ethylenediamine compound 3a by reductive amination using sodium triacetoxyborohydride (STAB) in 1,2-dichloroethane to generate compound 4a in an yield of 81%. Compound 4a was reacted with different substituted phenylboric acids 5a–g in the presence of catalyst 1,1′-di-tert-butylphosphinoferrocene palladium dichloride and base potassium carbonate in a mixed solvent (toluene/water/ethanol = 2/1/1) to generate compounds 6a–g in moderate yields (70%–85%). And then, compound 6a–g were converted to the precursor compound 7a–g by the required conditions: compound 6a was treated with sodium ethoxide and formamide to give amide compound 7a in a yield of 72%; compound 6a was reacted with hydrazine hydrate in ethanol to generate the hydrazide compound 7b in a yield of 61%; compounds 6b,c were converted to amino substituted compounds 7b,c using hydrazine hydrate and Pd/C by catalytic transfer hydrogenation in a moderate yield (67%–79%); compounds 6d–f were treated with hydroxylamine hydrochloride and triethylamine to give N-hydroxyamidino compounds 7e–g in moderated yields (54%–63%); compounds 6d–g were reacted with lithium bis(trimethylsilyl)amide and subsequently protection reaction to form protected amidino compounds 7h–k in a yield of 32%–92%. Finally, the target compounds 1a–k were obtained by deprotection of compounds 7a–k with the saturated hydrochloric acid ethanol solution in good yields (80%–90%).

The synthesis of amidino derivatives 1l-o is depicted in Scheme 2. The commercially available 5-bromofurfural was, respectively, treated with compounds 3a–d by reductive amination using sodium triacetoxyborohydride (STAB) in 1,2-dichloroethane to generate compounds 4a–d in moderate yields (70%–85%). And then, using potassium carbonate as a base and 1,1′-di-tert-butylphosphinoferrocene palladium dichloride as a catalyst, compounds 4a–d were reacted with 2-cyanopyridine-5-borate ester 5h in a mixed solvent (toluene/water/ethanol = 2/1/1) by the Suzuki reaction to generate compounds 9a–d in low yields (50%–60%). Subsequently, similar to the procedure in Scheme 1, compounds 9a–d were treated with the required reagents by the nucleophilic addition reaction to generate compounds 10a–d in moderate yields (50%–80%), which were deprotected with the saturated hydrochloric acid ethanol solution to afford the target compounds 1l–o in moderate yields (75%–85%).

A series of 2,4-substituted thiazole derivatives 1p–r were synthesized, as illustrated in Scheme 3. The commercially available 3-bromopyruvate ethyl was reacted with thiourea by cyclization reaction in absolute ethanol to generate 2-amino thiazole compound 13 in a yield of 93%. And then, compound 13 was treated with tert-butyl nitrite and copper dibromide in acetonitrile by diazotization and bromination reaction to give 2-bromo thiazole compound 14 in a low yield of 30%. Meanwhile, compound 14 was converted to aldehyde compound 15 sequentially by reductive reaction in the presence of sodium borohydride and oxidation reaction in the presence of the Dess–Martin reagent in a moderate yield of 72% over two steps. Similar to the procedure in Schemes 1, 2, compound 15 was, respectively, reacted with side chain 3a or 3d by reductive amination to generated compounds 16a,b in moderate yields (55%–75%), which were treated with boronic acid 5d or borate ester 5h by the Suzuki reaction to generate compounds 17a–c in moderate yields (55%–75%). Compounds 17a–c were treated in the required conditions by the nucleophilic addition reaction to generate compounds 18a–c in low yields (30%–70%), which were deprotected with the saturated hydrochloric acid ethanol solution to afford the target compounds 1p–r in good yields (85%–90%).

A series of 2,5-substituted furan and 2,4-substituted thiazole derivatives were synthesized and screened for their inhibitory effects on PRMT1 by using the radioactive PRMT1 methylation inhibition assay, which measured the amount of methyl groups that transferred from [³H]-SAM to a biotinylated histone H4 peptide [ac-SGRGKGGKGLGKGGAKRHRKVGGK(Biotin)] (Tables 1–3). In the assay, SAH and DB75 were used as the positive controls.

Initially, we designed and synthesized a series of molecules (1a–k), as shown in Table 1, which contained substituted phenyl on 5-furan and ethylenediamine side chain on 2-furan. The chemical modification of these compounds is mainly concentrated on the substituted benzene ring, such as amide, hydrazide, amino, and N-hydroxyamidino and amidino groups. Compared with compounds 1a–d, it was found that the inhibitory rate of 4-amino-substituted compound (1c) was stronger than those of amide, hydrazide, or 3-amino-substituted compounds (1a, 1b, and 1d) at 10 μM against PRMT1 and was selected for the IC₅₀ determination (21.42 ± 12.76 μM). It was shown that the introduction of the hydrogen bond donor group on the 4-phenyl group could improve the inhibitory activity slightly. However, it was noticed that introducing the N-hydroxyamidino group (the hydrogen bond donor group as well) on the benzene ring (compounds 1e, 1f, and 1g) could not increase the inhibitory activity, whose inhibition rates were only 32%–40% at 10 μM against PRMT1. Subsequently, four compounds (1h, 1i, 1j, and 1k), containing 4-amidino, 3-F-4-amidino, 3-amidino, and 3-amidino-4-Cl group, respectively, showed strong inhibitory effects (68%–77%) at 10 μM against PRMT1 and were selected for the IC₅₀ determinations, which were 2.66 ± 0.01, 4.42 ± 0.63, 2.06 ± 0.60, and 2.50 ± 0.91 μM, respectively. Although the IC₅₀s of these compounds are weaker than those of SAH and DB75, we found that introducing the hydrogen donor group, amidino on the benzene ring, could increase the inhibitory activity greatly (Table 1), which could be explained by the fact that the amidino group may mimic the guanidino group of substrate arginine.

In the second step, we assumed that the introduction of “N” into the benzene ring could increase the interaction between the compound and PRMT1; the benzene ring was transformed to the pyridine ring and different amino side chains were introduced to gain a series of molecules (1l–o), as shown in Table 2. Among them, it was found that four compounds (1l, 1m, 1n, and 1o) showed strong inhibitory rates (48%–92%) at 10 μM against PRMT1, and IC₅₀s were determined as 1.29 ± 0.12, 10.78 ± 1.30, 4.68 ± 0.26, and 2.41 ± 0.18 μM, respectively. Introducing the pyridine ring on the 5-furan could improve the inhibitory activity slightly by comparing compounds 1l and 1h–k. However, compounds 1m and 1n bearing the cyclopropyl group and the piperazine group on the R1 position, respectively, have the weaker inhibitory activity than 1l, probably because of the steric hindrance on the R1 position. Moreover, introducing the primary amine on the terminal amino group (1o) could have a similar inhibitory activity with 1l. The IC₅₀ of compound 1l is only about two times weaker than SAH and four times weaker than DB75 (Table 2). So far, we found that introducing the pyridine bearing the amidino group on the 5-furan could perform relatively good inhibitory activities.

In the third step, in order to investigate the effects of other cores such as thiazole on the inhibitory activity of PRMT1, we designed and synthesized a series of compounds (1p–r, Table 3) based on the structure of compound 1l. These three compounds showed relatively good inhibitory activities, and their inhibitory rates were higher than 75% at a concentration of 10 μM, and especially compounds 1p and 1r exhibited 1.49 ± 0.47 and 1.21 ± 0.11 μM IC₅₀s, respectively, against PRMT1. Among all of the above compounds, it should be noted that compounds 1p and 1r have similar inhibitory activities and their IC₅₀s are about two times weaker than SAH and three times weaker than DB75 (Table 3). In conclusion, among all the compounds, two most potent ones (1l and 1r) were discovered with almost identical inhibitory activities, and 1r was selected as the representative compound for the following study.

The selectivity profiles of compound 1r against PRMT1 and other type I PRMTs (PRMT3, 4, 6, and 8) were investigated, and the IC₅₀s were measured, as illustrated in Table 4. It can be observed that compound 1r exhibited similar inhibitory activities against PRMT4 and PRMT8, which are stronger than those against PRMT3 and 6.

Western blotting was performed to confirm that WCJ-394 altered the arginine methylation patterns of PRMT1 in A549 cells. As shown in Figure 2, after the treatment of WCJ-394 in A549 cells for 48 h, the expression of PRMT1 was not significantly affected, and the expression level of ADMA, which is mainly produced by type I PRMTs, was significantly reduced in a concentration-dependent manner. In addition, dimethylarginine dimethylaminohydrolase (DDAH), which can metabolize more than 90% of ADMA (Chen et al., 2009), was also significantly decreased in a concentration-dependent manner. Taken together, the decrease of ADMA was because of the inhibition of PRMT1 by WCJ-394, rather than being metabolized by DDAH or decreasing the expression of PRMT1, which suggested that WCJ-394 directly influenced the PRMT–ADMA pathway. Unlike ADMA, the expression of SDMA, which was catalyzed by type II PRMTs, was not affected by WCJ-394. In conclusion, it was confirmed that WCJ-394 was a PRMT1 inhibitor and had significant effects on the PRMT pathway at cellular levels.

It was reported that PRMT1 may activate the TGF-β/SMAD3 signaling pathway (Katsuno et al., 2018; Wei et al., 2019); therefore, the ability of WCJ-394 to inhibit TGF-β signaling was measured by using the mink lung epithelial cell (MLEC), which was stably transfected with a TGF-β-responsive plasminogen activator inhibitor-1 promoter-luciferase construct (Abe et al., 1994). On the addition of TGF-β, a dose-dependent increase in luciferase activity can be induced, so that the inhibitory activity of compounds on TGF-β signaling pathway can be evaluated (Wu et al., 2020). As shown in Figure 3, WCJ-394 could significantly inhibit the TGF-β signaling with an IC₅₀ of 17.71 μΜ, without showing significant cytotoxicity in a concentration up to 100 μΜ under the same condition.

To determine the effects of WCJ-394 on the inhibition of TGF-β1-induced epithelial–mesenchymal transition (EMT) in A549 cells, the characterized proteins were detected using the Western blotting assay. Initially, the potential cytotoxic effect of WCJ-394 was examined through the CCK-8 cell viability assay and the results (Figure 4) suggested that WCJ-394 had an IC₅₀ of 583.70 μM in A549 cells treated for 48 h and did not result in any cytotoxicity in this cell line at the concentrations of 0–50 μM.

As shown in Figure 5A, one of the hallmarks of EMT is the increased production of mesenchymal markers, such as fibronectin, N-cadherin, alpha-smooth muscle actin (α-SMA), and metalloproteinases (MMPs), which permits transdifferentiated cells to exhibit invasive and motile phenotypes. The effect of WCJ-394 on the expression of EMT-related proteins, including fibronectin, N-cadherin, MMP-2, and α-SMA in A549 cells induced by TGF-β1, was assessed by Western blotting. As shown in Figures 5B–E, the expression levels of those proteins were significantly (p < 0.01) increased in response to TGF-β1 and were decreased (p < 0.05) by the pretreatment of WCJ-394 in a concentration-dependent manner.

In order to investigate the effects of WCJ-394 on the TGF-β1-induced A549 cell migration, scratch wound healing and transwell assay were performed to determine the rate of wound closure and the number of migration cells, individually.

In the scratch wound healing assay, the percentages of scratch widths at 24 and 48 h compared to that of 0 h were used to denote the rate of A549 cell migration. As shown in Figures 6A–C, the TGF-β1 treatment promoted the scratched wound closure. While such TGF-β1-induced cell migration was significantly inhibited by the pretreatment of WCJ-394 at 10 or 20 μΜ at 24 h, and the retardation effect of WCJ-394 on cell migration was further detected at 48 h.

Additionally, similar results were obtained in the transwell migration assay (Figures 6D,E). The number of cells, which passed through the transwell membrane, was significantly increased after TGF-β1 treatment. WCJ-394 (5, 10, and 20 μΜ) pretreatment significantly decreased the number of migrated cells in a concentration-dependent manner.

The molecular docking was performed to study the binding pose of WCJ-394 against PRMT1. The crystal structure of PRMT1 complexed with GSK3368715 (a PRMT1 inhibitor) was recently solved, and the PDB ID is 6NT2 (Fedoriw et al., 2019), which was used for molecular docking, and the binding position of GSK3368715 was used to indicate the potential binding site. As Figure 7A shows, the molecular docking clearly indicated that WCJ-394 occupied the substrate arginine binding site and did not bind to the SAH binding site. By comparing with the binding modes of WCJ-394 and GSK3368715, it can be noticed that there are some slight differences (Figure 7B). For GSK3368715, the ethylenediamine group binds close to SAH, mimicking the guanidine group of the substrate arginine. However, for WCJ-394, the amidino group (not the ethylenediamine group) occupies the binding position of the guanidine group of the substrate arginine, which may be because the amidino group shares a higher similarity with the guanidine group. The interactions between WJC-394 and PRMT1 were analyzed, as shown in Figure 7C. It can be noticed that the amidino group of WJC-394 can form six hydrogen bonds with Glu163, Glu171, and Tyr57, and also can form strong electrostatic interactions with Glu162 and Glu171. The terminal amino group on the ethylenediamine group can form electrostatic interactions with Glu65 and can form hydrogen bonds with His311 and Glu65. The π–π interactions are mainly contributed by Trp312, Tyr166, His311, and Tyr57, and Ile62 and Met66 contribute significant hydrophobic interactions. It can also be noticed that the sulfur atom of SAH is close to the amidino group of WJC-394, which may positively contribute to the stabilization of the leading compound.

All reagents and solvents were purchased from the supplier and used directly in the experiment. Thin-layer chromatography (TLC) was performed on silica gel (silica gel 60 pre-coated aluminum plates from Macherey-Nagel). All ¹H and ¹³C NMR spectra were recorded on a Bruker Avance III 400 spectrometer. The solvents used for the NMR spectrum was CDCl₃, DMSO-d ₆, and D₂O, and TMS was used as the internal standard. Mass spectra were recorded on a Thermo TSQ Quantum Access Max. HRMS was recorded on an Agilent 6545 Q-TOF LC/MS. The ionization method was ESI (Elektron Spray Ionization). Melting point was measured on a WRS-1B digital melting point apparatus, uncorrected.

General procedure A: to a solution of 2 (2.66 mmol) in dichloroethane (20 ml) were added 3a–d (2.66 mmol) and sodium triacetoxyborohydride (5.32 mmol) at 0°C under argon and then stirred at room temperature for 12 h. The reaction solution was quenched with saturated NH₄Cl (20 ml), extracted with CH₂Cl₂ (30 ml × 3), and the combined organic layers were dried with Na₂SO₄. The residue was purified by column chromatography on silica gel using EtOAc/petroleum ether as an eluent to give 4a–d.

General procedure B: compound 4a (2.88 mmol) was dissolved in a mixed solvent (toluene/EtOH/H₂O = 2:1:1, 32 ml), and 5a–g (4.32 mmol) was added. And then, potassium carbonate (8.64 mmol) and 1,1′-di-tert-butylphosphinoferrocene palladium dichloride (0.058 mmol) were added to the reaction solution. The reaction mixture was heated at 80°C for 8 h. After the reaction was completed, it was quenched with saturated NH₄Cl (20 ml) and extracted with EtOAc (20 ml × 3). The combined organic layers were dried with Na₂SO₄, filtered, and concentrated. The residue was purified by column chromatography on silica gel using EtOAc/petroleum ether as an eluent to give 6a–g.

General procedure C: to a solution of compounds 6b,c (1.36 mmol) in isopropanol (30 ml) were added hydrazine hydrate (80%, 4 ml, 68 mmol) and Pd/C (10%, 0.30 g). The reaction solution was heated at 80°C for 5 h and filtered. The filtrated was concentrated, and the residue was purified by column chromatography on silica gel using EtOAc/petroleum ether as an eluent to give 7c,d.

General procedure D: to a solution of compounds 6d–f (2.98 mmol) in dry ethanol (50 ml) were added hydroxylamine hydrochloride (8.94 mmol) and triethylamine (8.94 mmol). The reaction mixture was stirred at room temperature for 29 h and concentrated. To the residue was added H₂O (30 ml) and extracted with EtOAc (10 ml × 3). The combined organic layers were dried with Na₂SO₄, filtered, and concentrated. The residue was purified by column chromatography on silica gel using 2% CH₃OH in CH₂Cl₂ as an eluent to give 7e–g.

General procedure E: to a solution of compounds 6d–g (2.71 mmol) in dry THF (20 ml) was added LiHMDS (13.55 mmol) at 0°C, and the reaction mixture was stirred at room temperature for 24 h. And then, the reaction solution was cooled to 0°C and the pH was adjusted to 2–3 using 2 N HCl and stirred at room temperature for 2 h. The reaction solution was concentrated and the pH was adjusted to 9–10 and extracted with CH₂Cl₂ (10 ml × 3). The combined organic layers were dried with Na₂SO₄, filtered, and concentrated to give the crude product. The crude product was dissolved in CH₂Cl₂ (30 ml), and triethylamine (9.75 mmol) was added. And then, (Boc)₂O (6.50 mmol) was added at 0°C and the reaction solution was stirred at room temperature for 15 h. After the reaction was completed, the reaction solution was washed with saturated NaHCO₃ (10 ml) and H₂O (10 ml). The organic layer was dried with Na₂SO₄, filtered, and concentrated. The residue was purified by column chromatography on silica gel using EtOAc/petroleum ether as an eluent to give 7h–k.

General procedure F: compounds 7a–k or 10a–d or 18a–c (2.00 mmol) were added to saturated hydrochloric acid in EtOH (20 ml); the resulting solution was stirred at room temperature for 5 h, and the reaction solution was concentrated. The residue was washed by Et₂O, and the precipitate was filtered to give 1a–r.

General procedure G: compounds 4a–d (1.15 mmol) were dissolved in a mixed solvent (toluene/EtOH/H2O = 2:1:1, 30 ml), and 5h (1.84 mmol) was added. And then, potassium carbonate (3.69 mmol) and 1,1′-di-tert-butylphosphinoferrocene palladium dichloride (0.06 mmol) were added to the reaction solution. The reaction solution was put into a microwave reactor and reacted in microwave for 0.5 h. After the reaction was completed, it was quenched with saturated NaCl (20 ml), and extracted with EtOAc (20 ml × 3). The combined organic layers were dried with Na₂SO₄, filtered, and concentrated. The residue was purified by column chromatography on silica gel using EtOAc/petroleum ether as an eluent to give 9a–d.

General procedure H: to a solution of compounds 9a–d (0.68 mmol) in methanol (10 ml) was added sodium methoxide (0.88 mmol) at 0°C, and the reaction solution was stirred for 0.5 h. And then, it was reacted at room temperature for 36 h, and ammonium chloride (0.88 mmol) was added and continued to stir for 24 h. After the reaction was completed, it was quenched with saturated NaCl (20 ml) and extracted with EtOAc (20 ml × 3). The combined organic layers were dried with Na₂SO₄, filtered, and concentrated. The residue was purified by silica gel column chromatography using CH₃OH in CH₂Cl₂ as the eluent to give 10a–d.

N-tert-Butyloxycarbonyl-N′-(5-bromofuran-2-yl-methyl)-N,N′-dimethylethylene Diamine (4a)

General procedure A. Yellow oil (750 mg, 81%); ¹H NMR (400 MHz, DMSO-d ₆) δ 6.49 (d, J = 3.2 Hz, 1H, ArH), 6.35 (d, J = 3.2 Hz, 1H, ArH), 3.52 (s, 2H, CH ₂ ), 3.29–3.20 (m, 2H, CH ₂ ), 2.76 (s, 3H, CH ₃ ), 2.42 (m, 2H, CH ₂ ), 2.18 (s, 3H, CH ₃ ), 1.45 [s, 9H, C(CH ₃)₃]; ES-MS 347.1 (M + H)⁺.

N-tert-Butoxycarbonyl-N-methyl-N′-(5-bromofuran-2-yl-methyl)-N′-cyclopropylethylenediamine (4b)

General procedure A. Yellow oil (900 mg, 78%); ¹H NMR (400 MHz, DMSO-d ₆) δ 6.50 (d, J = 3.7 Hz, 1H, ArH), 6.36 (dd, J = 7.2, 3.2 Hz, 1H, ArH), 3.71 (s, 2H, CH ₂ ), 3.21 (m, 2H, CH ₂ ), 2.74 (s, CH ₃ ), 2.64 (m, 2H, CH ₂ ), 1.84 [m, 1H, CH(CH₂)₂], 1.37 [s, 9H, C(CH ₃)₃], 0.44–0.41 [m, 2H, CH(CH ₂)₂], 0.29–0.26 [m, 2H, CH(CH ₂)₂]; ES-MS 373.1 (M + H)⁺.

N-tert-Butoxycarbonyl-N-methylene-N′-(5-bromofuran-2-yl-methyl)-piperazine (4c)

General procedure A. Yellow oil (1,100 mg, 80%); ¹H NMR (400 MHz, DMSO-d ₆) δ 6.50 (d, J = 3.3 Hz, 1H, ArH), 6.37 (d, J = 3.2 Hz, 1H, ArH), 3.49 (s, 2H, CH ₂ ), 3.29 [t, J = 5.1 Hz, 4H, N(CH ₂ )₂], 2.31 [t, J = 5.1 Hz, 4H, N(CH ₂ )₂], 1.38 [s, 9H, C(CH ₃ )₃]; ES-MS 345.1 (M + H)⁺.

N-tert-Butoxycarbonyl-N′-(5-bromofuran-2-yl-methyl)-N′-methylethylenediamine (4d)

General procedure A. Yellow oil (850 mg, 50%); ¹H NMR (400 MHz, DMSO-d ₆) δ 6.67 (t, J = 5.8 Hz, 1H, NH), 6.49 (d, J = 3.2 Hz, 1H, ArH), 6.35 (d, J = 3.3 Hz, 1H, ArH), 3.51 (s, 2H, CH ₂ ), 3.01 (m, 2H, CH ₂ ), 2.35 (m, 2H, CH ₂ ), 2.14 (s, 3H, CH ₃ ), 1.37 [s, 9H, C(CH ₃ )₃]; ES-MS 333.1 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(4-methoxycarbonylphenyl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine (6a)

General procedure B. Yellow oil (2.40 g, 82%); ¹H NMR (400 MHz, CDCl₃) δ 8.08–8.03 (m, 2H, ArH), 7.77–7.69 (m, 2H, ArH), 6.76 (d, J = 3.3 Hz, 1H, ArH), 6.36 (d, J = 3.4 Hz, 1H, ArH), 3.95 (s, 3H, CH ₃ ), 3.73 (s, 2H, CH ₂ ), 3.41 (m, 2H, CH ₂ ), 2.90 (s, 3H, CH ₃ ), 2.61 (m, 2H, CH ₂ ), 2.39 (s, 3H, CH ₃ ), 1.46 [s, 9H, (CH ₃ )₃]; ES-MS 403.3 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(4-nitrophenyl)-furan-2-yl-methyl]-N,N′-dimethyl Ethylenediamine (6b)

General procedure B. Yellow oil (0.42 g, 75%); ¹H NMR (400 MHz, DMSO-d ₆) δ 8.32–8.23 (m, 2H, ArH), 7.91 (d, J = 8.6 Hz, 2H, ArH), 7.27 (d, J = 3.3 Hz, 1H, ArH), 6.52 (d, J = 3.4 Hz, 1H, ArH), 3.66 (s, 2H, CH ₂ ), 3.35 (m, 2H, CH ₂ ), 2.78 (s, 3H, CH ₃ ), 2.48 (m, 2H, CH ₂ ), 2.26 (s, 3H, CH ₃ ), 1.35 [s, 9H, (CH ₃ )₃]; ES-MS 390.2 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(3-nitrophenyl)-furan-2-yl-methyl]-N,N′-dimethyl Ethylenediamine (6c)

General procedure B. Yellow oil (0.66 g, 73%); ¹H NMR (400 MHz, DMSO-d ₆) δ 8.41 (t, J = 2.0 Hz, 1H, ArH), 8.11 (dd, J = 8.3, 2.3 Hz, 2H, ArH), 7.72 (t, J = 8.0 Hz, 1H, ArH), 7.20 (d, J = 3.3 Hz, 1H, ArH), 6.48 (d, J = 3.3 Hz, 1H, ArH), 3.66 (s, 2H, CH ₂ ), 3.28 (m, 2H, CH ₂ ), 2.79 (s, 3H, CH ₃ ), 2.48 (m, 2H, CH ₂ ), 2.26 (s, 3H, CH ₃ ), 1.35 [s, 9H, (CH ₃ )₃]; ES-MS 390.2 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(4-cyanophenyl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine (6d)

General procedure B. Yellow oil (1.10 g, 89%); ¹H NMR (400 MHz, DMSO-d ₆) δ 7.89–7.80 (m, 4H, ArH), 7.18 (d, J = 3.3 Hz, 1H, ArH), 6.48 (d, J = 3.4 Hz, 1H, ArH), 3.64 (s, 2H, CH ₂ ), 3.27 (m, 2H, CH ₂ ), 2.78 (s, 3H, CH ₃ ), 2.47 (m, 2H, CH ₂ ), 2.25 (s, 3H, CH ₃ ), 1.35 [s, 9H, (CH ₃ )₃]; ES-MS 370.2 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(3-fluoro-4-cyanophenyl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine (6e)

General procedure B. Yellow oil (0.68 g, 76%); ¹H NMR (400 MHz, DMSO-d ₆) δ 7.95 (dd, J = 8.2, 6.9 Hz, 1H, ArH), 7.77 (d, J = 10.9 Hz, 1H, ArH), 7.67 (d, J = 8.2 Hz, 1H, ArH), 7.29 (d, J = 3.4 Hz, 1H, ArH), 6.51 (d, J = 3.4 Hz, 1H, ArH), 3.65 (s, 2H, CH ₂ ), 3.33–3.23 (m, 2H, CH ₂ ), 2.77 (s, 3H, CH ₃ ), 2.47 (m, 2H, CH ₂ ), 2.25 (s, 3H, CH ₃ ), 1.35 [s, 9H, (CH ₃ )₃]; ES-MS 388.2 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(3-cyanophenyl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine (6f)

General procedure B. Yellow oil (0.94 g, 72%); ¹H NMR (400 MHz, DMSO-d ₆) δ 8.12 (d, J = 1.7 Hz, 1H, ArH), 7.97 (d, J = 7.9 Hz, 1H, ArH), 7.72 (dt, J = 7.7, 1.4 Hz, 1H, ArH), 7.62 (t, J = 7.8 Hz, 1H, ArH), 7.11 (d, J = 3.3 Hz, 1H, ArH), 6.45 (d, J = 3.3 Hz, 1H, ArH), 3.63 (s, 2H, CH ₂ ), 3.32 (m, 2H, CH ₂ ), 2.77 (m, 3H, CH ₃ ), 2.49 (m, 2H, CH ₂ ), 2.25 (s, 3H, CH ₃ ), 1.35 [s, 9H, (CH ₃ )₃]; ES-MS 370.2 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(3-cyano-4-chlorophenyl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine (6g)

General procedure B. Yellow oil (0.88 g, 76%); ¹H NMR (400 MHz, DMSO-d ₆) δ 8.25 (d, J = 2.2 Hz, 1H, ArH), 7.97 (dd, J = 8.6, 2.2 Hz, 1H, ArH), 7.78 (d, J = 8.6 Hz, 1H, ArH), 7.15 (d, J = 3.3 Hz, 1H, ArH), 6.46 (d, J = 3.4 Hz, 1H, ArH), 3.63 (s, 2H, CH ₂ ), 3.27 (m, 2H, CH ₂ ), 2.77 (s, 3H, CH ₃ ), 2.47 (m, 2H, CH ₂ ), 2.25 (s, 3H, CH ₃ ), 1.35 [s, 9H, (CH ₃ )₃]; ES-MS 404.2 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(4-formamide-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine (7a)

To a solution of compound 6a (562 mg, 1.40 mmol) in DMF (2 ml) in the sealed tube were added sodium ethoxide (95 mg, 1.40 mmol) and formamide (1 ml, 24.80 mmol). The reaction solution was heated at 100°C for 2 h. After cooling, to the reaction solution was added saturated NaHCO₃ (10 ml) and extracted with CH₂Cl₂ (10 ml × 3). The combined organic layers were dried with Na₂SO₄, filtered, and concentrated. The residue was purified by column chromatography on silica gel using 3% CH₃OH in CH₂Cl₂ as an eluent to give 7a as a yellow oil (390 mg, 72%); ¹H NMR (400 MHz, CDCl₃) δ 7.89–7.80 (m, 2H, ArH), 7.75–7.68 (m, 2H, ArH), 6.72 (d, J = 3.3 Hz, 1H, ArH), 6.33 (d, J = 3.4 Hz, 1H, ArH), 6.09 (s, 3H, NH ₂ ), 3.68 (s, 2H, CH ₂ ), 3.36 (m, 2H, CH ₂ ), 2.88 (s, 3H, CH ₃ ), 2.59 (m, 2H, CH ₂ ), 2.37 (s, 3H, CH ₃ ), 1.46 [s, 9H, (CH ₃ )₃]; ES-MS 388.2 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(4-formylhydrazide-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine (7b)

To a solution of compound 6a (510 mg, 1.27 mmol) in ethanol (30 ml) was added hydrazine hydrate (80%, 5.40 ml, 101.60 mmol). The reaction solution was heated at 80°C for 30 h. After cooling, to the reaction solution was added H₂O (10 ml) and extracted with CH₂Cl₂ (10 ml × 3). The combined organic layers were dried with Na₂SO₄, filtered, and concentrated. The residue was purified by column chromatography on silica gel using 4% CH₃OH in CH₂Cl₂ as an eluent to give 7b as a colorless oil (310 mg, 61%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.80 (s, 1H, NH), 7.87 (d, J = 8.5 Hz, 2H, ArH), 7.72 (d, J = 8.2 Hz, 2H, ArH), 7.02 (d, J = 3.3 Hz, 1H, ArH), 6.43 (d, J = 3.3 Hz, 1H, ArH), 4.50 (s, 2H, NH ₂ ), 3.63 (s, 2H, CH ₂ ), 3.30–3.26 (m, 2H, CH ₂ ), 2.78 (s, 3H, CH ₃ ), 2.48 (m, 2H, CH ₂ ), 2.25 (s, 3H, CH ₃ ), 1.36 [s, 9H, (CH ₃ )₃]; ES-MS 403.2 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(4-aminophenyl)-furan-2-yl-methyl]-N,N′-dimeth-ylethylenediamine (7c)

General procedure C. Yellow oil (0.33 g, 67%); ¹H NMR (400 MHz, DMSO-d ₆) δ 7.32 (d, J = 8.4 Hz, 2H, ArH), 6.63–6.53 (m, 2H, ArH), 6.46 (d, J = 3.2 Hz, 1H, ArH), 6.27 (d, J = 3.2 Hz, 1H, ArH), 5.28 (s, 2H, NH ₂ ), 3.54 (s, 2H, CH ₂ ) 3.31–3.23 (m, 2H, CH ₂ ), 2.77 (s, 3H, CH ₃ ), 2.45 (m, 2H, CH ₂ ), 2.22 (s, 3H, CH ₃ ), 1.37 [s, 9H, (CH ₃ )₃]; ES-MS 360.2 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(3-aminophenyl)-furan-2-yl-methyl]-N,N′-dimeth-ylethylenediamine (7d)

General procedure C. Yellow oil (0.45 g, 79%); ¹H NMR (400 MHz, DMSO-d ₆) δ 7.03 (t, J = 7.8 Hz, 1H, ArH), 6.90–6.79 (m, 2H, ArH), 6.67 (d, J = 3.2 Hz, 1H, ArH), 6.47 (ddd, J = 8.0, 2.3, 1.0 Hz, 1H, ArH), 6.34 (d, J = 3.3 Hz, 1H, ArH), 5.17 (s, 2H, NH ₂ ), 3.59 (s, 2H, CH ₂ ), 3.33–3.22 (m, 2H, CH ₂ ), 2.78 (s, 3H, CH ₃ ), 2.47 (m, 2H, CH ₂ ), 2.24 (s, 3H, CH ₃ ), 1.37 [s, 9H, (CH ₃ )₃]; ES-MS 360.2 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(4-N-hydroxyamidino-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine (7e)

General procedure D. Yellow oil (0.65 g, 54%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.69 (s, 1H, OH), 7.71 (d, J = 8.6 Hz, 2H, ArH), 7.65 (d, J = 8.5 Hz, 2H, ArH), 6.92 (d, J = 3.3 Hz, 1H, ArH), 6.40 (d, J = 3.3 Hz, 1H, ArH), 5.83 (s, 2H, NH ₂ ), 3.62 (s, 2H, CH ₂ ), 3.28 (m, 2H, CH ₂ ), 2.78 (s, 3H, CH ₃ ), 2.48 (m, 2H, CH ₂ ), 2.25 (s, 3H, CH ₃ ), 1.36 [s, 9H, (CH ₃ )₃]; ES-MS 403.2 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(3-fluoro-4-N-hydroxyamidino-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine (7f)

General procedure D. Yellow oil (0.50 g, 54%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.71 (s, 1H, OH), 7.59–7.45 (m, 3H, ArH), 7.05 (d, J = 3.3 Hz, 1H, ArH), 6.43 (d, J = 3.3 Hz, 1H, ArH), 5.83 (s, 2H, NH ₂ ), 3.63 (s, 2H, CH ₂ ), 3.28 (m, 2H, CH ₂ ), 2.77 (s, 3H, CH ₃ ), 2.47 (m, 2H, CH ₂ ), 2.25 (s, 3H, CH ₃ ), 1.36 [s, 9H, (CH ₃ )₃]; ES-MS 421.2 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′'-[5-(3-N-hydroxyamidino-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine (7g)

General procedure D. Yellow oil (0.44 g, 63%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.70 (s, 1H, OH), 7.97 (t, J = 1.7 Hz, 1H, ArH), 7.66 (d, J = 7.8 Hz, 1H, ArH), 7.57 (dt, J = 7.9, 1.3 Hz, 1H, ArH), 7.41 (t, J = 7.8 Hz, 1H, ArH), 6.91 (d, J = 3.2 Hz, 1H, ArH), 6.41 (d, J = 3.3 Hz, 1H, ArH), 5.90 (s, 2H, NH ₂ ), 3.62 (s, 2H, CH ₂ ), 3.28 (m, 2H, CH ₂ ), 2.78 (s, 3H, CH ₃ ), 2.47 (m, 2H, CH ₂ ), 2.25 (s, 3H, CH ₃ ), 1.36 [s, 9H, (CH ₃ ) ₃ ]; ES-MS 403.2 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(4-N-tert-butyloxycarbonylamidino-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine (7h)

General procedure E. Yellow oil (0.24 g, 92%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.07 (s, 2H, NH), 8.06–7.98 (m, 2H, ArH), 7.75 (d, J = 8.6 Hz, 2H, ArH), 7.06 (d, J = 3.3 Hz, 1H, ArH), 6.45 (d, J = 3.3 Hz, 1H, ArH), 3.64 (s, 2H, CH ₂ ), 3.28 (m, 2H, CH ₂ ), 2.78 (s, 3H, CH ₃ ), 2.48 (m, 2H, CH ₂ ), 2.26 (s, 3H, CH ₃ ), 1.46 [s, 9H, C(CH ₃ )₃], 1.36 (s, 9H, C(CH ₃ )₃); ES-MS 487.3 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(3-fluoro-4-N-tert-butyloxycarbonylamidino-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine (7i)

General procedure E. Yellow oil (0.30 g, 79%); ¹H NMR (400 MHz, DMSO-d ₆) δ 8.94 (s, 1H, NH), 8.57 (s, 1H, NH), 7.67 (t, J = 8.0 Hz, 1H, ArH), 7.60–7.52 (m, 2H, ArH), 7.13 (d, J = 3.3 Hz, 1H, ArH), 6.46 (d, J = 3.4 Hz, 1H, ArH), 3.64 (s, 2H, CH ₂ ), 3.28 (m, 2H, CH ₂ ), 2.78 (s, 3H, CH ₃ ), 2.47 (m, 2H, CH ₂ ), 2.25 (s, 3H, CH ₃ ), 1.43 [s, 9H, C(CH ₃ )₃], 1.39 [s, 9H, C(CH ₃ )₃]; ES-MS 505.3 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(3-N-tert-butyloxycarbonylamidino-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine (7j)

General procedure E. Yellow oil (0.28 g, 84%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.07 (s, 2H, NH), 8.20 (s, 1H, ArH), 7.89–7.79 (m, 2H, ArH), 7.51 (t, J = 7.8 Hz, 1H, ArH), 6.97 (d, J = 3.3 Hz, 1H, ArH), 6.43 (d, J = 3.3 Hz, 1H, ArH), 3.64 (s, 2H, CH ₂ ), 3.29 (m, 2H, CH ₂ ), 2.78 (s, 3H, CH ₃ ), 2.48 (m, 2H, CH ₂ ), 2.26 (s, 3H, CH ₃ ), 1.46 [s, 9H, C(CH ₃ )₃], 1.36 [s, 9H, C(CH ₃ )₃]; ES-MS 487.3 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[5-(3-N-tert-butyloxycarbonylamidino-4-chloro-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine (7k)

General procedure E. Yellow oil (0.22 g, 32%); ¹H NMR (400 MHz, DMSO-d ₆) δ 8.67 (s, 1H, NH), 8.50 (s, 1H, NH), 7.73–7.68 (m, 1H, ArH), 7.65 (p, J = 3.8 Hz, 1H, ArH), 7.53 (d, J = 8.4 Hz, 1H, ArH), 7.05 (d, J = 3.3 Hz, 1H, ArH), 6.42 (d, J = 3.4 Hz, 1H, ArH), 3.62 (s, 2H, CH ₂ ), 3.27 (m, 2H, CH ₂ ), 2.77 (s, 3H, CH ₃ ), 2.47 (m, 2H, CH ₂ ), 2.24 (s, 3H, CH ₃ ), 1.44 [s, 9H, C(CH ₃ )₃], 1.27 [s, 9H, C(CH ₃ )₃]; ES-MS 521.3 (M + H)⁺.

N-tert-Butoxycarbonyl-N′-[5-(2-cyano-5-pyridyl)furan-2-yl-methyl]-N,N′-dimethylethylenediamine (9a)

General procedure G. Yellow oil (0.50 g, 59%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.07 (d, J = 2.1 Hz, 1H, ArH), 8.21 (dd, J = 8.2, 2.1 Hz, 1H, ArH), 8.06 (d, J = 8.2 Hz, 1H, ArH), 7.34 (d, J = 3.4 Hz, 1H, ArH), 6.54 (d, J = 3.4 Hz, 1H, ArH), 3.67 (s, 2H, CH ₂), 3.28 (m, 2H, CH ₂), 2.78 (s, 3H, CH ₃), 2.48 (m, 2H, CH ₂), 2.26 (s, 3H, CH ₃), 1.35 [s, 9H, (CH ₃ ) ₃ ]; ES-MS 371.2 (M + H)⁺.

N-tert-Butoxycarbonyl-N-methyl-N′-[5-(2-cyano-5-pyridyl)furan-2-yl-methyl]-N′-cyclopropylethylenediamine (9b)

General procedure G. Yellow oil (0.50 g, 59%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.07 (d, J = 2.2 Hz, 1H, ArH), 8.21 (d, J = 8.2 Hz, 1H, ArH), 8.06 (d, J = 8.3 Hz, 1H, ArH), 7.35 (d, J = 3.4 Hz, 1H, ArH), 6.56 (d, J = 3.4 Hz, 1H, ArH), 3.84 (s, 2H, CH ₂), 3.28 (m, 2H, CH ₂), 2.76 (s, 3H, CH ₃), 2.69 (m, 2H, CH ₂), 1.93 [m, 1H, CH(CH₂)₂], 1.37 [s, 9H, (CH ₃)₃], 0.47–0.44 [m, 2H, CH(CH ₂)₂], 0.34–0.28 [m, 2H, CH(CH ₂)₂]; ES-MS 397.2 (M + H)⁺.

N-tert-Butoxycarbonyl-N-methylene-N′-[5-(2-cyano-5-pyridyl)furan-2-yl-methyl]-piperazine (9c)

General procedure G. Yellow oil (0.65 g, 55%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.08 (d, J = 2.2 Hz, 1H, ArH), 8.23 (dd, J = 8.3, 2.2 Hz, 1H, ArH), 8.07 (d, J = 8.2 Hz, 1H, ArH), 7.35 (d, J = 3.4 Hz, 1H, ArH), 6.56 (d, J = 3.4 Hz, 1H, ArH), 3.63 (s, 2H, CH ₂), 2.67 [t, J = 4.8 Hz, 4H, N (CH ₂)₂], 2.39 [t, J = 5.0 Hz, 4H, N(CH ₂)₂], 1.38 [s, 9H, (CH ₃)₃]; ES-MS 369.2 (M + H)⁺.

N-tert-Butoxycarbonyl-N′-[5-(2-cyano-5-pyridyl)furan-2-yl-methyl]-N′-methylethylenediamine (9d)

General procedure G. Yellow oil (0.40 g, 45%);¹H NMR (400 MHz, DMSO-d ₆) δ 9.08 (d, J = 2.2 Hz, 1H, ArH), 8.23 (dd, J = 8.2, 2.3 Hz, 1H, ArH), 8.06 (d, J = 8.2 Hz, 1H, ArH), 7.34 (d, J = 3.4 Hz, 1H, ArH), 6.70 (t, J = 5.9 Hz, 1H, NH), 6.56 (d, J = 3.4 Hz, 1H, ArH), 3.70 (s, 2H, CH ₂), 3.08 (m, 2H, CH ₂), 2.47 (m, 2H, CH ₂), 2.26 (s, 3H, CH ₃), 1.36 [s, 9H, (CH ₃)₃]; ES-MS 357.2 (M + H)⁺.

N-tert-Butoxycarbonyl-N′-[5-(2-amidino-5-pyridyl)furan-2-yl-methyl]-N,N′-dimethylethylenediamine (10a)

General procedure H. Yellow oil (0.12 g, 80%); ¹H NMR (400 MHz, DMSO-d ₆) 9.55 (s, 1H, NH), 9.32 (s, 2H, NH ₂), 9.10 (s, 1H, ArH), 8.35 (d, J = 1.6 Hz, 2H, ArH), 7.39 (d, J = 3.4 Hz, 1H, ArH), 6.56 (d, J = 3.4 Hz, 1H, ArH), 3.67 (s, 2H, CH ₂), 3.28 (m, 2H, CH ₂), 2.78 (s, 3H, CH ₃), 2.48 (m, 2H, CH ₂), 2.27 (s, 3H, CH ₃), 1.35 [s, 9H, (CH ₃)₃]; ES-MS 388.2 (M + H)⁺.

N-tert-Butoxycarbonyl-N-methyl-N′-[5-(2-amidino-5-pyridyl)furan-2-yl-methyl]-N′-cyclopropylethylenediamine (10b)

General procedure H. Yellow oil (0.12 g, 55%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.62 (s, 1H, NH), 9.22 (s, 2H, NH ₂), 9.09 (d, J = 2.1 Hz, 1H, ArH), 8.48 (d, J = 8.4 Hz, 1H, ArH), 8.31 (dd, J = 8.4, 2.2 Hz, 1H, ArH), 7.39 (d, J = 3.4 Hz, 1H, ArH), 6.55 (d, J = 3.4 Hz, 1H, ArH), 3.84 (s, 2H, CH ₂), 3.31–3.22 (m, 2H, CH ₂), 2.75 (s, 3H, CH ₃), 2.69 (m, 2H, CH ₂), 1.99–1.86 [m, 1H, CH(CH₂)₂], 1.35 [s, 9H, (CH ₃)₃], 0.46 [m, 2H, CH(CH ₂)₂], 0.30 [m, 2H, CH(CH ₂)₂]; ES-MS 414.3(M + H)⁺.

N-tert-Butoxycarbonyl-N-methylene-N′-[5-(2-amidino-5-pyridyl)furan-2-yl-methyl]-piperazine (10c)

General procedure H. Yellow powder (0.18 g, 52%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.51 (s, 2H, NH ₂), 9.17 (s, 1H, NH), 9.12 (dd, J = 2.2, 0.8 Hz, 1H, ArH), 8.36 (dd, J = 8.4, 2.2 Hz, 1H, ArH), 8.31 (d, J = 8.4 Hz, 1H, ArH), 7.39 (d, J = 3.4 Hz, 1H, ArH), 6.58 (d, J = 3.4 Hz, 1H, ArH), 3.64 (s, 2H, CH ₂), 3.32 [s, 4H, N(CH ₂)₂], 2.41 [s, 4H, N(CH ₂)₂], 1.38 [s, 9H, (CH ₃)₃]; ES-MS 386.2 (M + H)⁺.

N-tert-Butoxycarbonyl-N′-[5-(2-amidino-5-pyridyl)furan-2-yl-methyl]-N′-methylethylenediamine (10d)

General procedure H. Yellow powder (0.18 g, 52%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.59 (s, 2H, NH ₂), 9.43 (s, 1H, NH), 9.11 (d, J = 2.1 Hz, 1H, ArH), 8.42-8.31 (m, 2H, ArH and ArH), 7.40 (d, J = 3.4 Hz, 1H, ArH), 6.74 (d, J = 6.1 Hz, 1H, NH), 6.56 (d, J = 3.4 Hz, 1H, ArH), 3.67 (s, 2H, CH ₂), 3.07 (m, 2H, CH ₂), 2.43 (m, 2H, CH ₂), 2.23 (s, 3H, CH ₃), 1.35 [s, 9H, (CH ₃)₃]; ES-MS 374.2 (M + H)⁺.

Ethyl 2-Aminothiazole-4-carboxylate (13)

To a solution of thiourea 12 (5.00 g, 65.69 mmol) in absolute ethanol (70 ml) was added ethyl bromopyruvate 11 (12.80 g, 72.26 mmol), and the reaction mixture was stirred at 78°C for 2.5 h. The reaction mixture was cooled to 0°C, crystallized, and filtered to give product 13 as a yellow solid (10.52 g, 93%). Without purification, it was proceeded directly to the next reaction.

Ethyl 2-Bromothiazole-4-carboxylate (14)

To a solution of 13 (5.00 g, 29.03 mmol) and CuBr₂ (9.63 g, 43.11 mmol) in acetonitrile (150 ml) was added tert-butyl nitrite (5.70 ml) slowly to the reaction solution at 0°C, and then the reaction solution was reacted for 2.5 h. After the reaction was completed, CH₂Cl₂/H₂O (50 ml/50 ml) was added, and then concentrated hydrochloric acid was added dropwise to adjust the pH to 1. The solution was extracted with CH₂Cl₂ (30 ml × 3), and the combined organic layers were dried with Na₂SO₄, filtered, and concentrated. The residue was purified by silica gel column chromatography using EtOAc/petroleum ether as an eluent to give 14 as a white solid (2.05 g, 30%), m.p. 66.4–68.2°C; ¹H NMR (400 MHz, DMSO-d ₆) δ 8.54 (s, 1H, ArH), 4.31 (q, J = 7.1 Hz, 2H, OCH ₂), 1.31 (t, J = 7.1 Hz, 3H, CH₂CH ₃); ES-MS 236.0 (M + H)⁺.

2-Bromothiazole-4-carbaldehyde (15)

Compound 14 (1.08 g, 4.57 mmol) was dissolved in ethanol (35 ml), NaBH₄ (0.35 g, 9.14 mmol) was added to the reaction solution at 0°C, and reacted for 0.5 h. The reaction solution was stirred at room temperature for 3.5 h and next at 70°C for 6 h. After the reaction was completed, it was quenched with saturated NaCl (20 ml) and extracted with EtOAc (30 ml × 3). The combined organic layers were dried with Na₂SO₄, filtered, and concentrated to a crude product, which was proceeded directly to the next reaction without purification.

The crude product was dissolved in anhydrous tetrahydrofuran (20 ml), and the Dess–Martin oxidant (5.47 mmol) was added; then, the reaction solution was stirred at room temperature for 2 h. The reaction solution was quenched with saturated NaCl (20 ml) and extracted with EtOAc (30 ml × 3). The organic layers were dried with Na₂SO₄, filtered, and concentrated. The residue was purified by column chromatography on silica gel using EtOAc/petroleum ether as an eluent to give 15 as a white solid (0.63 g, 72%), m.p. 120.4–123.6°C; ¹H NMR (400 MHz, DMSO-d ₆) δ 9.83 (s, 1H, CHO), 8.74 (s, 1H, ArH); ES-MS 191.9 (M + H)⁺.

N-tert-Butoxycarbonyl-N′-(2-bromothiazol-4-yl-methyl)-N,N′-dimethylethylenediamine (16a)

General procedure A. Yellow solid (1.31 g, 55%); ¹H NMR (400 MHz, DMSO-d ₆) δ 7.48 (s, 1H, ArH), 3.63 (s, 2H, CH ₂), 3.25 (m, 2H, CH ₂), 2.79 (s, 3H, CH ₃), 2.47 (m, 2H, CH ₂), 2.22 (s, 3H, CH ₃), 1.37 [s, 9H, (CH ₃)₃]; ES-MS 364.1 (M + H)⁺.

N-tert-Butoxycarbonyl-N′-(2-bromothiazol-4-yl-methyl)-N′-methylethylenediamine (16b)

General procedure A. Yellow oil (0.73 g, 77%); ¹H NMR (400 MHz, DMSO-d ₆) δ 7.51 (s, 1H, ArH), 6.68 (t, J = 5.8 Hz, 1H, NH), 3.61 (s, 2H, CH ₂ ), 3.04 (m, 2H, CH ₂ ), 2.40 (m, 2H, CH ₂ ), 2.19 (s, 3H, CH ₃ ), 1.37 [s, 9H, (CH ₃ )₃]; ES-MS 350.1 (M + H)⁺.

N-tert-Butoxycarbonyl-N′-[2-(4-cyanophenyl)thiazol-4-yl-methyl]-N,N′-dimethylethylenediamine (17a)

General procedure B. Yellow oil (0.87 g, 87%); ¹H NMR (400 MHz, DMSO-d ₆) δ 8.11 (d, J = 2.0 Hz, 2H, ArH), 7.96 (d, J = 1.6 Hz, 2H, ArH), 7.63 (s, 1H, ArH), 3.76 (s, 2H, CH ₂), 3.30 (s, 3H, CH ₃), 2.79 (s, 3H, CH ₃), 2.55 (m, 2H, CH ₂), 2.30 (m, 2H, CH ₂), 1.35 [s, 9H, (CH ₃)₃]; ES-MS 387.2 (M + H)⁺.

N-tert-Butoxycarbonyl-N′-[2-(2-cyano-5-pyridyl)thiazol-4-yl-methyl]-N,N′-dimethylethylenediamine (17b)

General procedure G. Yellow oil (0.55 g, 70%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.28 (d, J = 2.2 Hz, 1H, ArH), 8.52 (dd, J = 8.2, 2.3 Hz, 1H, ArH), 8.16 (d, J = 8.2 Hz, 1H, ArH), 7.72 (s, 1H, ArH), 3.75 (s, 2H, CH ₂), 3.30 (m, 2H, CH ₂), 2.79 (s, 3H, CH ₃), 2.55 (m, 2H, CH ₂), 2.27 (s, 3H, CH ₃), 1.36 [s, 9H,(CH ₃)₃]; ES-MS 388.2 (M + H)⁺.

N-tert-Butoxycarbonyl-N′-[2-(2-cyano-5-pyridyl)thiazol-4-yl-methyl]-N′-methylethylenediamine (17c)

General procedure G. Yellow oil (0.45 g, 65%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.36 (d, J = 2.2 Hz, 1H, ArH), 8.60 (dd, J = 8.2, 2.0 Hz, 1H, ArH), 8.23 (d, J = 8.2 Hz, 1H, ArH), 7.84 (s, 1H, ArH), 6.75 (t, J = 6.0 Hz, 1H, NH), 3.83 (s, 2H, CH ₂), 3.17 (m, 2H, CH ₂), 2.54 (m, 2H,CH ₂), 2.34 (s, 3H, CH ₃), 1.44 [s, 9H, (CH ₃)₃]; ES-MS 3374.2 (M + H)⁺.

N-tert-Butyloxycarbonyl-N′-[2-(4-N-tert-butyloxycarbonylamidino-phen-1-yl]-thiazol-4-yl-methyl)-N,N′-dimethylethylenediamine (18a)

General procedure E. Yellow oil (80 mg, 29%); 1H NMR (400 MHz, DMSO-d ₆) δ 9.07 (s, 1H, NH), 8.07 (d, J = 8.7 Hz, 2H, ArH), 8.01 (d, J = 8.6 Hz, 2H, ArH), 5.76 (s, 1H, ArH), 3.73 (s, 2H, CH ₂), 3.29 (m, 2H, CH ₂), 2.79 (s, 3H, CH ₃), 2.29 (s, 3H, CH ₃), 1.45 [s, 9H, (CH ₃)₃], 1.35 [s, 9H, (CH ₃)₃]; ES-MS 504.2 (M + H)⁺.

N-tert-Butoxycarbonyl-N′-[2-(2-amidino-5-pyridyl)thiazol-4-yl-methyl]-N,N′-dimethylethylenediamine (18b)

General procedure H. Yellow oil (0.10 g, 72%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.69 (s, 3H, NH and NH ₂), 9.30 (d, J = 2.2 Hz, 1H, ArH), 8.64 (dd, J = 8.3, 2.3 Hz, 1H, ArH), 8.52 (d, J = 8.4 Hz, 1H, ArH), 7.78 (s, 1H, ArH), 3.85 (s, 2H, CH ₂), 3.51 (m, 2H, CH ₂), 2.79 (s, 3H, CH ₃), 2.59 (m, 2H, CH ₂), 2.35 (s, 3H, CH ₃), 1.35 [s, 9H, (CH ₃)₃]; ES-MS 405.2 (M + H)⁺.

N-tert-Butoxycarbonyl-N′-[2-(2-amidino-5-pyridyl)thiazol-4-yl-methyl]-N′-methylethylenediamine (18c)

General procedure H. Yellow oil (0.12 g, 70%); ¹H NMR (400 MHz, DMSO-d ₆) δ 9.74 (s, 2H, NH ₂), 9.31 (d, J = 2.2 Hz, 1H, NH), 8.64 (dd, J = 8.3, 2.2 Hz, 1H, ArH), 8.52 (d, J = 8.3 Hz, 1H, ArH), 8.19 (d, J = 1.9 Hz, 1H, ArH), 7.80 (s, 1H, ArH), 6.75 (t, J = 5.7 Hz, 1H, NH), 3.82 (s, 2H, CH ₂), 3.11 (m, 2H, CH ₂), 2.59 (m, 2H,CH ₂), 2.30 (s, 3H, CH ₃), 1.35 [s, 9H, (CH ₃)₃].

N′-[5-(4-Formamide-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine Hydrochloride (1a)

General procedure F. Yellow solid (0.26 g, 52%); m.p. 212.2–214.6°C; ¹H NMR (400 MHz, D₂O) δ 7.66–7.58 (m, 4H, ArH), 6.81 (d, J = 3.5 Hz, 1H, ArH), 6.75 (d, J = 3.5 Hz, 1H, ArH), 4.45 (s, 2H, CH ₂ ), 3.49 (m, 2H, CH ₂), 3.43 (m, 2H, CH ₂), 2.85 (s, 3H, CH ₃ ), 2.67 (s, 3H, CH ₃ ); ¹³C NMR (101 MHz, D₂O) δ 172.27, 155.06, 142.57, 132.93, 131.86, 128.12, 124.01, 117.71, 108.55, 52.38, 49.92, 42.79, 39.91, 33.23; HRMS (ESI) m/z [M + H]⁺ calcd for C₁₆H₂₂N₃O₂ ⁺: 288.1707, found: 288.1706.

N′-[5-(4-Formylhydrazide-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine Hydrochloride (1b)

General procedure F. Yellow solid (0.22 g, 84%); m.p. 246.2–246.9°C; ¹H NMR (400 MHz, D₂O) δ 7.82–7.73 (m, 4H, ArH), 6.93 (d, J = 3.5 Hz, 1H, ArH), 6.79 (d, J = 3.5 Hz, 1H, ArH), 4.48 (s, 2H, CH ₂ ), 3.53–3.48 (m, 2H, CH ₂ ), 3.45–3.41 (m, 2H, CH ₂ ), 2.86 (s, 3H, CH ₃ ), 2.67 (s, 3H, CH ₃ ); ¹³C NMR (101 MHz, D₂O) δ 168.07, 154.70, 142.75, 133.75, 128.76, 128.24, 124.12, 117.70, 108.96, 52.30, 49.92, 42.75, 39.82, 33.19; HRMS (ESI) m/z [M + H]⁺ calcd for C₁₆H₂₃N₄O₂ ⁺: 303.1816, found: 303.1816.

N′-[5-(4-Aminophenyl)-furan-2-yl-methyl]-N,N′-dimeth-ylethylenediamine Hydrochloride (1c)

General procedure F. Yellow solid (0.25 g, 95%); m.p. 60.2–61.1°C; ¹H NMR (400 MHz, D₂O) δ 7.76 (d, J = 8.7 Hz, 2H, ArH), 7.33 (d, J = 8.7 Hz, 2H, ArH), 6.84 (d, J = 3.5 Hz, 1H, ArH), 6.77 (d, J = 3.5 Hz, 1H, ArH), 4.47 (s, 2H, CH ₂ ), 3.54–3.46 (m, 2H, CH ₂ ), 3.46–3.39 (m, 2H, CH ₂ ), 2.85 (s, 3H, CH ₃ ), 2.67 (s, 3H, CH ₃ ); ¹³C NMR (101 MHz, D₂O) δ 154.91, 142.33, 131.11, 125.52, 123.06, 118.03, 117.60, 107.61, 52.39, 49.92, 42.80, 39.86, 33.21; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₅H₂₂N₃O⁺: 260.1757, found: 260.1756.

N′-[5-(3-Aminophenyl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine Hydrochloride (1d)

General procedure F. Yellow solid (0.48 g, 95%); m.p. 64.2–65.2°C; ¹H NMR (400 MHz, D₂O) δ 7.71 (dd, J = 8.0, 1.4 Hz, 1H, ArH), 7.59 (s, 1H, ArH), 7.46 (t, J = 7.7 Hz, 1H, ArH), 7.21 (ddt, J = 8.0, 2.2, 1.1 Hz, 1H, ArH), 6.86 (d, J = 3.5 Hz, 1H, ArH), 6.78 (d, J = 3.5 Hz, 1H, ArH), 4.52 (s, 2H, CH ₂ ), 3.52–3.46 (m, 2H, CH ₂ ), 3.46–3.39 (m, 2H, CH ₂ ), 2.85 (s, 3H, CH ₃ ), 2.67 (s, 3H, CH ₃ ); ¹³C NMR (101 MHz, D₂O) δ 154.52, 142.55, 131.33, 131.22, 130.76, 124.21, 122.31, 118.09, 117.63, 108.12, 52.39, 49.97, 42.80, 39.87, 33.24; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₅H₂₂N₃O⁺: 260.1757, found: 260.1756.

N′-[5-(4-N-Hydroxyamidino-phen-1-yl)-furan-2-yl-meth-yl]-N,N′-dimethylethylenediamine Hydrochloride (1e)

General procedure F. Yellow solid (0.51 g, 81%); m.p. 231.2–232.8°C; ¹H NMR (400 MHz, D₂O) δ 7.85 (d, J = 8.6 Hz, 2H, ArH), 7.68 (d, J = 8.6 Hz, 2H, ArH), 6.99 (d, J = 3.5 Hz, 1H, ArH), 6.83 (d, J = 3.5 Hz, 1H, ArH), 4.51 (s, 2H, CH ₂ ), 3.56–3.50 (m, 2H, CH ₂ ), 3.48–2.44 (m, 2H, CH ₂ ), 2.89 (s, 3H, CH ₃ ), 2.70 (s, 3H, CH ₃ ); ¹³C NMR (101 MHz, D₂O) δ 161.16, 154.49, 143.14, 134.32, 128.29, 124.59, 124.09, 117.76, 109.33, 52.36, 50.02, 42.81, 39.90, 33.25; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₆H₂₃N₄O₂ ⁺: 303.1816, found: 303.1805.

N′-[5-(3-Fluoro-4-N-hydroxyamidino-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine Hydrochloride (1f)

General procedure F. Yellow solid (0.39 g, 91%); m.p. 216.8–218.6°C; ¹H NMR (400 MHz, D₂O) δ 7.66–7.57 (m, 3H, ArH), 7.00 (d, J = 3.6 Hz, 1H, ArH), 6.83 (d, J = 3.6 Hz, 1H, ArH), 4.49 (s, 2H, CH ₂ ), 3.54–3.50 (m, 2H, CH ₂ ), 3.48–3.42 (m, 2H, CH ₂ ), 2.87 (s, 3H, CH ₃ ), 2.70 (s, 3H, CH ₃ ); ¹³C NMR (101 MHz, D₂O) δ 160.99, 158.47, 156.49, 153.30, 143.45, 136.20, 136.11, 130.44, 120.34, 117.80, 111.95, 111.83, 111.71, 110.24, 52.22, 50.02, 42.76, 39.82, 33.22; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₆H₂₂FN₄O₂ ⁺: 321.1721, found: 321.1715.

N′-[5-(3-N-Hydroxyamidino-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine Hydrochloride (1g)

General procedure F. White solid (0.41 g, 69%); m.p. 60.8–63.2°C; ¹H NMR (400 MHz, D₂O) δ 7.96–7.88 (m, 2H, ArH), 7.55–7.50 (m, 2H, ArH), 6.87 (d, J = 3.5 Hz, 1H, ArH), 6.77 (d, J = 3.5 Hz, 1H, ArH), 4.45 (s, 2H, CH ₂), 3.52–3.44 (m, 2H, CH ₂), 3.43–3.39 (m, 2H, CH ₂), 2.83 (s, 3H, CH ₃), 2.66 (s, 3H, CH ₃); ¹³C NMR (101 MHz, D₂O) δ 161.20, 154.40, 142.61, 130.61, 130.09, 128.95, 127.05, 125.62, 122.98, 117.71, 108.22, 52.41, 50.03, 42.85, 39.89, 33.29; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₆H₂₃N₄O₂ ⁺: 303.1816, found: 303.1811.

N′-[5-(4-Amidino-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine Hydrochloride (1h)

General procedure F. White solid (330 mg, 81%); m.p. 186.5–188.9°C; ¹H NMR (400 MHz, D₂O) δ 7.82 (d, J = 8.6 Hz, 2H, ArH), 7.72 (d, J = 8.6 Hz, 2H, ArH), 6.96 (d, J = 3.5 Hz, 1H, ArH), 6.80 (d, J = 3.5 Hz, 1H, ArH), 4.47 (s, 2H, CH ₂ ), 3.52–3.48 (m, 2H, CH ₂ ), 3.45–3.40 (m, 2H, CH ₂ ), 2.85 (s, 3H, CH ₃ ), 2.67 (s, 3H, CH ₃ ); ¹³C NMR (101 MHz, D₂O) δ 166.14, 154.49, 143.31, 134.54, 128.50, 126.96, 124.45, 117.73, 109.44, 52.40, 50.06, 42.89, 39.97, 33.28; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₆H₂₃N₄O⁺: 287.1866, found: 287.1869.

N′-[5-(3-Fluoro-4-amidino-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine Hydrochloride (1i)

General procedure F. Yellow solid (300 mg, 80%); m.p.156.5–159.2°C; ¹H NMR (400 MHz, D₂O) δ 7.70–7.55 (m, 3H, ArH), 7.00 (d, J = 3.6 Hz, 1H, ArH), 6.82 (d, J = 3.5 Hz, 1H, ArH), 4.49 (s, 2H, CH ₂ ), 3.55–3.48 (m, 2H, CH ₂ ), 3.47–3.41 (m, 2H, CH ₂ ), 2.86 (s, 3H, CH ₃ ), 2.69 (s, 3H, CH ₃ ); ¹³C NMR (101 MHz, D₂O) δ 162.07, (161.17, 158.65), (153.42, 153.39), 143.56, (136.31, 136.21), 130.54, (120.26, 120.23), 117.80, (115.13, 115.01), (111.98, 111.74), 110.27, 52.27, 50.05, 42.80, 39.87, 33.23; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₆H₂₂FN₄O⁺: 305.1772, found: 305.1773.

N′-[5-(3-Amidino-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine Hydrochloride (1j)

General procedure F. Orange oil (310 mg, 85%); ¹H NMR (400 MHz, D₂O) δ 7.99 (td, J = 1.9, 0.7 Hz, 1H, ArH), 7.96 (dt, J = 7.7, 1.5 Hz, 1H, ArH), 7.61 (dt, J = 7.9, 1.5 Hz, 1H, ArH), 7.55 (td, J = 7.8, 0.6 Hz, 1H, ArH), 6.89 (d, J = 3.5 Hz, 1H, ArH), 6.78 (d, J = 3.5 Hz, 1H, ArH), 4.45 (s, 2H, CH ₂ ), 3.51–3.44 (m, 2H, CH ₂ ), 3.45–3.38 (m, 2H, CH ₂ ), 2.83 (s, 3H, CH ₃ ), 2.67 (s, 3H, CH ₃ ); ¹³C NMR (101 MHz, D₂O) δ 166.51, 154.51, 142.54, 130.45, 129.91, 129.11, 128.68, 127.22, 123.14, 117.68, 108.12, 52.37, 49.96, 42.78, 39.82, 33.23; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₆H₂₃N₄O⁺: 287.1866, found: 287.1867.

N′-[5-(3-Amidino-4-chloro-phen-1-yl)-furan-2-yl-methyl]-N,N′-dimethylethylenediamine Hydrochloride (1k)

General procedure F. Yellow solid (290 mg, 81%); m.p. 223–225°C; ¹H NMR (400 MHz, D₂O) δ 7.90–7.85 (m, 2H, ArH), 7.60 (d, J = 8.5 Hz, 1H, ArH), 6.91 (d, J = 3.5 Hz, 1H, ArH), 6.83 (d, J = 3.5 Hz, 1H, ArH), 4.52 (s, 2H, CH ₂ ), 3.62–3.50 (m, 2H, CH ₂ ), 3.52–3.42 (m, 2H, CH ₂ ), 2.89 (s, 3H, CH ₃ ), 2.72 (s, 3H, CH ₃ ); ¹³C NMR (101 MHz, D₂O) δ 164.65, 153.39, 143.92, 130.96, 130.16, 129.02, 128.78, 128.44, 124.70, 117.01, 108.36, 52.39, 50.10, 43.19, 39.95, 33.15; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₆H₂₂ClN₄O⁺: 321.1477, found: 321.1465.

N′-[5-(2-Amidino-5-pyridyl)furan-2-yl-methyl]-N,N′-dimethylethylenediamine Hydrochloride (1l)

General procedure F. Yellow solid (200 mg, 70%); m.p. 75.8–78.2°C; ¹H NMR (400 MHz, D₂O) δ 9.03 (dd, J = 2.2, 0.8 Hz, 1H, ArH), 8.28 (dd, J = 8.4, 2.2 Hz, 1H, ArH)), 8.04 (dd, J = 8.4, 0.8 Hz, 1H, ArH), 7.14 (d, J = 3.6 Hz, 1H, ArH), 6.88 (d, J = 3.6 Hz, 1H, ArH), 4.53 (s, 2H, CH ₂ ), 3.54 (m, 2H, CH ₂ ), 3.51–3.44 (m, 2H, CH ₂ ), 2.89 (s, 3H, CH ₃ ), 2.72 (s, 3H, CH ₃ ); ¹³C NMR (101 MHz, D₂O) δ162.11, 151.75, 145.44, 144.21, 142.06, 132.78, 129.78, 123.33, 117.75, 111.05, 52.16, 50.05, 42.77, 39.80, 33.17; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₅H₂₂N₅O⁺: 288.1824, found: 288.1811.

N-Methyl-N′-[5-(2-amidino-5-pyridyl)furan-2-yl-methyl]-N′-cyclopropylethylenediamine Hydrochloride (1m)

General procedure F. Yellow solid (90 mg, 80%); m.p. 95.9–98.3°C; ¹H NMR (400 MHz, D₂O) δ 8.96 (dd, J = 2.3, 0.8 Hz, 1H, ArH), 8.21 (dd, J = 8.4, 2.2 Hz, 1H, ArH), 7.98 (dd, J = 8.4, 0.9 Hz, 1H, ArH), 7.08 (d, J = 3.6 Hz, 1H, ArH), 6.80 (d, J = 3.5 Hz, 1H, ArH), 4.52 (s, 2H, CH ₂), 3.56–3.50 (m, 2H, CH ₂), 3.49–3.42 (m, 2H, CH ₂), 2.74 (tt, J = 7.3, 4.0°Hz, 1H, CH), 2.66 (s, 3H, CH ₃), 0.86 (dd, J = 7.1, 1.8°Hz, 2H, CH ₂), 0.75 (dd, J = 4.3, 2.0°Hz, 2H, CH ₂); ¹³C NMR (101 MHz, D₂O) δ 162.01, 151.40, 145.40, 144.79, 141.86, 132.68, 129.82, 123.32, 117.64, 111.25, 51.92, 49.96, 42.92, 37.45, 33.24, 4.60; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₇H₂₄N₅O⁺: 314.1975, found: 314.1966.

N′-[5-(2-Amidino-5-pyridyl)furan-2-yl-methyl]-piperazine Hydrochloride (1n)

General procedure F. Yellow solid (200 mg, 85%); m.p. 197.7–199.3°C; ¹H NMR (400 MHz, D₂O) δ 8.96 (dd, J = 2.2, 0.9 Hz, 1H, ArH), 8.21 (dd, J = 8.4, 2.2 Hz, 1H, ArH), 7.98 (dd, J = 8.4, 0.9 Hz, 1H, ArH), 7.06 (d, J = 3.5 Hz, 1H, ArH), 6.77 (d, J = 3.6 Hz, 1H, ArH), 4.38 (s, 2H, CH ₂), 3.44 [s, 4H, (CH ₂)₂], 3.43 [s, 4H, (CH ₂)₂]; ¹³C NMR (101 MHz, D₂O) δ 162.09, 151.60, 145.40, 144.38, 141.96, 132.71, 129.80, 123.32, 117.46, 111.05, 52.30, 47.83, 40.86, 40.20; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₅H₂₀N₅O⁺: 286.1662, found: 286.1659.

N′-[5-(2-Amidino-5-pyridyl)furan-2-yl-methyl]-N′-methylethylenediamine Hydrochloride (1o)

General procedure F. Yellow solid (200 mg, 85%); m.p. 223.4–224.8°C; ¹H NMR (400 MHz, D₂O) δ 9.01 (s, 1H, ArH), 8.24 (d, J = 8.2 Hz, 1H, ArH), 8.02 (d, J = 8.6 Hz, 1H, ArH), 7.07 (s, 1H, ArH), 6.53 (s, 1H, ArH), 3.71 (s, 2H, CH ₂), 3.01 (m, 2H, CH ₂), 2.68 (m, 2H, CH ₂), 2.27 (s, 3H, CH ₃); ¹³C NMR (101 MHz, D₂O) δ 153.11, 149.12, 144.90, 140.54, 131.77, 130.43, 123.25, 112.67, 110.92, 52.64, 52.46, 40.78, 36.45; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₄H₂₀N₅O⁺: 274.1662, found: 274.1671.

N′-[2-(4-Amidino-phen-1-yl)thiazol-4-yl-methyl]-N,N′-dimethylethylenediamine (1p)

General procedure F. Gray powder (200 mg, 85%); m.p. 97.6–98.5°C; ¹H NMR (400 MHz, D₂O) δ 8.07–8.02 (m, 2H, ArH), 7.86 (s, 1H, ArH), 7.82–7.77 (m, 2H, ArH), 4.53 (s, 2H, CH ₂), 3.62–3.54 (m, 2H, CH ₂), 3.49 (m, 2H, CH ₂), 2.88 (s, 3H, CH ₃), 2.69 (s, 3H, CH ₃); ¹³C NMR (101 MHz, D₂O) δ 168.72, 165.92, 144.37, 136.79, 129.53, 128.59, 127.14, 125.36, 54.63, 50.31, 42.69, 40.08, 33.13; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₅H₂₂N₅S⁺: 304.1590, found: 304.1600.

N′-[2-(2-Aminocarboximido-5-pyridyl)thiazol-4-yl-methyl]-N,N′-dimethylethylenediamine Hydrochloride (1q)

General procedure F. Yellow solid (90 mg, 82%); m.p. 188.7–190.2°C; ¹H NMR (400 MHz, D₂O) δ 9.23 (dd, J = 2.3, 1.1 Hz, 1H, ArH), 8.54–8.49 (m, 1H, ArH), 8.15 (dd, J = 8.3, 1.2 Hz, 1H, ArH), 7.80 (s, 1H, ArH), 4.14 (s, 2H, CH ₂), 3.34 (m, CH ₂), 3.15 (m, 2H, CH ₂), 2.70 (s, 3H, CH ₃), 2.55 (s, 3H, CH ₃); ¹³C NMR (101 MHz, D₂O) δ 164.53, 161.81, 147.43, 144.24, 135.94, 132.44, 123.40, 55.16, 51.00, 44.43, 40.51, 32.88; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₄H₂₁N₆S⁺: 305.1543, found: 305.1546.

N′-[2-(2-Aminocarboximido-5-pyridyl)thiazol-4-yl-methyl]-N′-methylethylenediamine Hydrochloride (1r)

General procedure F. Yellow solid (120 mg, 90%); m.p. 252–254°C; ¹H NMR (400 MHz, D₂O) δ 9.21 (dd, J = 2.3, 0.8 Hz, 1H, ArH), 8.50 (dd, J = 8.4, 2.2 Hz, 1H, ArH), 8.11 (dd, J = 8.3, 0.8 Hz, 1H, ArH), 7.96 (s, 1H, ArH), 4.58 (s, 2H, CH ₂), 3.57 (m, 2H, CH ₂), 3.48–3.43 (m, 2H, CH ₂), 2.91 (s, 3H, CH ₃); ¹³C NMR (101 MHz, D₂O) δ 165.48, 160.87,147.71, 145.37, 144.76, 136.32, 132.48, 126.07, 123.58, 54.76, 51.80, 40.25, 34.03; HRMS (ESI) m/z (M + H)⁺ calcd for C₁₃H₁₉N₆S⁺: 291.1386, found: 291.1380.

The enzyme inhibitory activities and selectivity were measured by the radioisotope assay and AlphaLISA assay in ShangHai Chempartner Co., Ltd., according to the standard protocol. The radioactive methylation assay (Sun et al., 2019) was performed in a 1× assay buffer (modified tris buffer) system containing enzyme (PRMT1), peptide, and [³H]-SAM solution and compounds on the assay plate. After 250 nL of compound solutions were added to the assay plate, 15 μl of PRMT1 enzyme solution or ×1 assay buffer for negative control was transferred to each well of prepared compound stock plates and the whole system (the final concentration of PRMT1 was 0.5 nM or 2 nM in the system) was incubated for 15 min at room temperature. Then, 10 μl of peptide and (³H)-SAM mixed solution were added to each well to start the reaction (the final concentration of (³H)-SAM was 0.25 μM in the system) and the reaction was incubated for 60 min at room temperature. Afterward, the reaction was stopped with the addition of 5 μl cold SAM solution to each well. Then, 25 μl volume per well was transferred to a Flashplate from the assay plate and incubated for a minimum of 1 h at room temperature. Finally, the Flashplate was washed with dH₂O and 0.1% Tween-20 three times and then the reading plate in Microbeta using the program ³H-Flashplate. The data were analyzed in GraphPad Prism 5 to obtain IC₅₀ values.

The selectivity of 1r (WCJ-394) against other type I PRMTs, including PRMT3, PRMT4, PRMT6, and PRMT8, was assessed using the AlphaLISA assay. In the AlphaLISA assay, firstly both the enzyme and substrate solution were prepared in a ×1 assay buffer, while compound 1r was transferred to the assay plate by Echo in a final concentration of 1% DMSO. Next, 5 μl of enzyme solution was transferred to the assay plate and incubated at room temperature for 15 min. Then, 5 μl of substrate solution was added to each well to start the reaction. After incubation at room temperature for 30 min (PRMT4,6) and 60 min (PRMT3,8), 15 μl of acceptor and donor bead mix solution were added to the assay plate and incubated at room temperature for 60 min with subdued light. The signal was collected with Envision or EnSpire with Alpha mode. The data were analyzed in GraphPad Prism 5 to obtain IC₅₀ values.

The human lung epithelial cell line A549 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, United States). Mink lung epithelial cells (MLECs) stably transfected with a TGF-β-responsive plasminogen activator inhibitor-1 promoter-luciferase construct were a kind gift from Dr. D Rifkin. The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), plus 1% penicillin and streptomycin (for A549 cells) or 200 μg/ml G418 (for MLEC) in a 37°C humidified environment containing 5% CO₂.

Cells were seeded into a 96-well plate; after 12 h of attachment, the cells were treated with different concentrations (0–1,600 μΜ) of WCJ-394 for 48 h. Cell viability was assessed by a Cell Counting Kit-8 (CCK-8; MCE) according to the manufacturer’s instructions. Briefly, after treatment, the CCK-8 solution was added to the culture medium and incubated at 37°C for 1 h. Cell viability was assessed by measuring the absorbance at 450 nm. In the subsequent luciferase assays, the effects of WCJ-394 on the cell viability were examined in parallel to exclude the compound-induced cell death.

The ability of WCJ-394 to inhibit TGF-β signaling was assayed using MLEC stably transfected with a TGF-β-responsive plasminogen activator inhibitor-1 promoter-luciferase construct (Abe et al., 1994). Briefly, cells were seeded into a 96-well plate at a density of 1 × 10⁴ cells per well in DMEM with 10% FBS and incubated at 37°C for 6 h to adhere to the surface. The medium was changed into DMEM with 0.1% FBS. The cells were treated with different concentrations (0–100 μΜ) of WCJ-394 for 30 min before 0.05 ng/μl TGF-β1 (Peprotech) was added to the cells and then incubated for another 18 h. The final concentration of DMSO should be maintained at 0.1%. After incubation, the medium was removed and the cells were rinsed with PBS to remove any residual compound. One hundred microliters of the Steady-Glo^® Luciferase Assay reagent (Promega) was added to each well, and the cells were lysed in the dark for 10 min on a horizontal shaker. The luciferase activity was measured in a luminometer. The effect of WCJ-394 on MLEC cell viability was assessed by CCK-8 simultaneously.

A549 cells in the logarithmic growth phase were seeded into a 6-well cell culture plate at a density of 3 × 10⁵ cells per well. After an overnight incubation at 37°C in a 5% CO₂ incubator, the medium was changed into the fresh DMEM with 0.1% FBS and the cells were incubated for another 24 h. The cells were treated with different concentrations (5–20 μΜ) of WCJ-394 for 2 h, with or without following the treatment of 5 ng/ml TGF-β1, and then incubated for another 48 h to measure the expression of EMT-associated proteins via the Western blotting assay. Additionally, A549 cells were treated with different concentrations of WCJ-394 (5–20 μΜ) to determine the expression of the PRMT 1-related protein.

A549 cells were lysed in RIPA buffer, which contains protease and phosphatase inhibitors, and then harvested in Eppendorf tubes for centrifugation (14,000 rpm for 10 min at 4°C) to remove cell debris. The supernatants were transferred into new Eppendorf tubes, and the protein concentration was quantified with a BCA kit (Thermo Fisher). The protein samples were separated by 10% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes. The membranes were blocked for 1 h at room temperature in 5% BSA and then incubated with the corresponding primary antibodies overnight at 4°C and then incubated with secondary antibodies for another 1 h at room temperature. After being washed with TBST buffer three times, the PVDF membranes were visualized with ECL reagents (Millipore) and the Western blot results were further analyzed by ImageJ software (National Institutes of Health).

A549 cells in the logarithmic growth phase were seeded into 6-well plates at a density of 6 × 10⁵ cell per well and incubated at 37°C in a 5% CO₂ incubator till reaching 100% confluence as a monolayer. The monolayer cell was scratched with a new 10 μl pipette tip across the center of the well, and the detached cells were gently rinsed with PBS buffer. The wells were refilled with a fresh DMEM medium that contains 0.1% FBS and the corresponding concentration of WCJ-394 to make the final concentration of 0, 5, 10, and 20 μΜ. After pretreatment with or without WCJ-394 for 2 h, TGF-β1 was added into the wells to a final concentration of 5 ng/ml and the cells were then incubated at 37°C in a 5% CO₂ incubator for another 48 h. The scratch was photographed through a microscope at ×100 magnification at 0, 24, and 48 h. Then, the scratch width was quantified by ImageJ software and the rate of wound closure was calculated with the final values expressed as a migration percentage of the scratch width relative to 0 h.

A549 cells in the logarithmic growth phase were resuspended with a serum-free DMEM medium and seeded into the 8-μm transwell filter inserts at a density of 8 × 10³ cells per well; meanwhile, 600 μl medium containing 10% FBS was added into the lower chambers of the 24-well cell culture plate. The corresponding concentration of WCJ-394 was added into both the upper and lower chambers to reach a final concentration of 0, 5, 10, and 20 μΜ and incubated for 2 h. After the pretreatment of WCJ-394, the TGF-β1 was added as a chemoattractant into the lower chambers to a final concentration of 5 ng/ml and incubated for an additional 24 h at 37°C. After incubation, the cells in the upper chambers were carefully removed with a cotton swab, while the cells that had traversed to the bottom side of the membrane were fixed with 4% paraformaldehyde for 15 min and stained with 0.1% crystal violet for 20 min. The chambers were washed with PBS buffer to remove extra crystal violet. Three fields of view were randomly taken and photographed from the central and surrounding parts of each membrane under a microscope at ×100 magnification, and cell number was calculated by ImageJ software.

All the quantitative data are shown as mean ± SD from at least three independent experiments. Data were analyzed by using GraphPad Prism 8.0. Comparison of means of multiple groups was performed by a one-way analysis of variance (ANOVA). p-value below 0.05 was considered to be statistically significant.

In the current study, the newly resolved crystal structure (PDB ID 6NT2) (Abe et al., 1994) of human PRMT1 complexed with GSK3368715 (a PRMT1 inhibitor) was downloaded from the Protein Data Bank. GOLD (Jones et al., 1997) (v5.2; Genetic Optimisation for Ligand Docking) was used for the docking study by referring the binding site of GSK3368715. Each compound was docked for 10 times, starting each time from a different random population of ligand orientations and using the default automatic genetic algorithm parameter settings. All torsion angles in each compound were allowed to rotate freely.