AUTHOR=Bogetofte Helle , Jensen Pia , Ryding Matias , Schmidt Sissel I. , Okarmus Justyna , Ritter Louise , Worm Christina S. , Hohnholt Michaela C. , Azevedo Carla , Roybon Laurent , Bak Lasse K. , Waagepetersen Helle , Ryan Brent J. , Wade-Martins Richard , Larsen Martin R. , Meyer Morten 

TITLE=PARK2 Mutation Causes Metabolic Disturbances and Impaired Survival of Human iPSC-Derived Neurons

JOURNAL=Frontiers in Cellular Neuroscience

VOLUME=Volume 13 - 2019

YEAR=2019

URL=https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2019.00297

DOI=10.3389/fncel.2019.00297

ISSN=1662-5102

ABSTRACT=The protein parkin, encoded by the PARK2 gene, is vital for mitochondrial homeostasis, and although it has been implicated in Parkinson’s disease (PD), the disease mechanisms remain unclear. We have applied mass spectrometry-based proteomics to investigate the effects of parkin dysfunction on the mitochondrial proteome in human isogenic induced pluripotent stem cell (iPSC)-derived neurons with and without PARK2 knockout (KO). The proteomic analysis quantified nearly 60% of all mitochondrial proteins, 119 of which were dysregulated in neurons with PARK2 KO. The protein changes indicated disturbances in oxidative stress defense, mitochondrial respiration and morphology, cell cycle control  and cell viability. Structural and functional analyses revealed an increase in mitochondrial area and the presence of elongated mitochondria as well as impaired glycolysis and lactate-supported respiration in PARK2 KO neurons. Furthermore, PARK2 KO caused decreased proliferation and impaired cell survival. This demonstrates the efficiency of mitochondrial proteomics for identifying and elucidating disease-relevant phenotypes in human iPSC-derived neurons.