There was a mistake in Figure 1D as published. When we conducted the Western blots to test the protein level of PCNA, Bax, Bcl2, Caspase3 in cells under different concentration of MPA treatment, the same housekeeping protein (tubulin) was used. However, when we exhibited the representative images in Figure 1D and Figure 2F, the identical picture of tubulin was presented. To avoid the unwanted misunderstandings, we replace the representative images in Figure 1D as follows. Of note, the image replacement DO NOT alter the protein gray values showing in Figure 1F. The corrected Figure 1D appears below.
Effect of MPA on the proliferation of PGK12.1 cell line. (A) Morphological changes of PGK12.1 cell line after 24 h of MPA treatment (scale bar, 100μm); (B) Effects of different concentrations of MPA on cell viability and proliferation inhibition rate of PGK12.1 cells after 24 h, 48 h, and 72 h of treatment. (C) Detection of mRNA expression levels of cell proliferation related genes PCNA, CDK1, and CCND1 by RT-QPCR. (D) Detection of cell proliferation related protein PCNA expression by Western Blot; (E) Quantitative analysis of grayscale values of protein bands. * Indicates a statistically significant difference compared to the corresponding control group, with * representing P < 0.05, ** representing P < 0.01, *** representing P < 0.001, **** representing P < 0.0001, and ns indicating no difference and no statistical significance.
Western blot image showing protein expression under MPA intervention. PCNA bands at 34 kilodaltons decrease with increasing concentrations from 0 to 0.1. Tubulin serves as a loading control at 55 kilodaltons, indicating consistent expression across samples.
There was a mistake in Figure 4.1C as published. When we typeset the images, parts of pictures showing in Figure 2A were unconsciously repeated presented in Figure 4.1C. Therefore, we replaced the improper images in Figure 4.1C as follows. Of note, the right images were used to access the fluorescence intensity of ROS, so the image replacement DO NOT alter the statistical result showing in Figure 4.1D. The corrected Figure 4.1C appears below.
The rescue effect of GUO on MPA treated PGK12.1 cell line. (A) Cell viability detection after treating PGK12.1 with different concentrations of GUO + MPA (0.075 uM) for 24 hours. (B) The effect of GUO on MPA induced morphological changes in PGK12.1 ESCs (scale bar, 200μm). (C) Fluorescent staining results of intracellular ROS content (scale bar, 100μm). (D) Analysis of ROS fluorescence intensity. (E) JC-1 detection of mitochondrial membrane potential fluorescent staining results in PGK12.1 ESCs (scale bar, 100μm). (F) Analysis of the red/green fluorescence intensity ratio of mitochondrial membrane potential. * representing P < 0.05, ** representing P < 0.01, *** representing P < 0.001.
Five panels showing fluorescent green specks scattered across a dark background. Each panel displays varying densities and distributions of the green specks, indicative of fluorescence intensity in microscopic samples.
There was a mistake in Table 1 as published. When we typeset the table contents, parts of the RT-qPCR primers were missing. Therefore, we supplement the information in the following Table 1. The corrected Table 1 appears below.
The original version of this article has been updated.
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