AUTHOR=Sahil  , Chaudhary Anis Ahmad , Sorout Sonia , Yadav Kushi , Ali Mohamed A. M. , Boufahja Fehmi , Kumar Vikram , Kothari SL , Jain Devendra , Verma Kumar Sambhav 

TITLE=Auxin-mediated biosynthesis of silver nanoparticles: comprehensive characterisation and antibacterial activity analysis

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=Volume 15 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2025.1678489

DOI=10.3389/fcimb.2025.1678489

ISSN=2235-2988

ABSTRACT=BackgroundSilver nanoparticles (AgNPs) are well-known for their potent antibacterial properties. However, the rise of antibiotic-resistant bacteria highlights the need for alternative antimicrobial strategies. Green synthesis using biological molecules offers an eco-friendly route to nanoparticle production.ObjectiveThis study aims to synthesise AgNPs using plant growth hormones (Auxins), specifically indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA), and to evaluate their antibacterial activity against pathogenic bacteria.MethodsAgNPs were synthesised using IAA and IBA as reducing and stabilising agents. The synthesised nanoparticles were characterised by UV-Visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Particle Size Analysis, Dynamic Light Scattering (DLS) for hydrodynamic diameter, Zeta potential for surface charge, and Field Emission Scanning Electron Microscopy (FE-SEM) for morphological analysis. Antibacterial assays were performed against Staphylococcus aureus and Escherichia coli.ResultsThe IAA and IBA-mediated AgNPs showed controlled size distribution, stability, and uniform morphology. Enhanced antibacterial activity was observed, particularly against S. aureus, compared to E. coli.ConclusionIAA and IBA-mediated synthesis provide a green, sustainable method for producing AgNPs with significant antibacterial potential. These Auxin-based AgNPs represent promising candidates for combating antibiotic-resistant bacterial strains.