Front. Cell. Infect. Microbiol.Frontiers in Cellular and Infection MicrobiologyFront. Cell. Infect. Microbiol.2235-2988Frontiers Media S.A.10.3389/fcimb.2025.1635286Cellular and Infection MicrobiologyCorrectionCorrection: Bid-Induced Release of AIF/EndoG from Mitochondria Causes Apoptosis of Macrophages during Infection with Leptospira interrogansHuWei-Lin12†DongHai-Yan3†LiYang12OjciusDavid M.4LiShi-Jun5*YanJie12*1Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, China2Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China3Department of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, China4Department of Biomedical Sciences, University of the Pacific, Arthur Dugoni School of Dentistry, San Francisco, CA, United States5Institute of Communicable Disease Control and Prevention, Guizhou Provincial Centre for Disease Control and Prevention, Guiyang, China
Edited and Reviewed by: Alain Filloux, Nanyang Technological University, Singapore
*Correspondence: Shi-Jun Li, zjumedjun@163.com; Jie Yan, med_bp@zju.edu.cn
†These authors have contributed equally to this work
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
A Correction on Bid-Induced Release of AIF/EndoG from Mitochondria Causes Apoptosis of Macrophages during Infection with Leptospira interrogans By Hu W-L, Dong H-Y, Li Y, Ojcius DM, Li S-J and Yan J (2017). Front. Cell. Infect. Microbiol. 7:471. doi: 10.3389/fcimb.2017.00471apoptosisLeptospiraBidAIFEndoGmacrophagesection-in-acceptanceMolecular Bacterial Pathogenesis
In the published article, there was an error in Figure 1E as published. The representative flow dot plots were misplaced. The corrected Figure 1 appears below.
Apoptosis, pathological changes, and MMP decrease in leptospire-infected macrophages. (A) Apoptosis of macrophages infected with L. interrogans strain Lai at the optimal apoptosis-causing time for different MOIs. J774A.1 and THP-1 cells were infected with leptospires at 4 h. Bars show the mean ± SD of three independent experiments. Five thousand cells were analyzed in each specimen. *p < 0.05 vs. apoptotic ratios in leptospire-infected macrophages with an MOI of 10, 50, 100, or 500. (B) Apoptosis of macrophages infected with L. interrogans strain Lai at the optimal apoptosis-causing MOI for different times. J774A.1 and THP-1 cells were infected at an MOI of 100. Bars show the mean ± SD of three independent experiments. Five thousand cells were analyzed in each specimen. *p < 0.05 vs. apoptotic ratios in each macrophage type before infection. (C) The representative pathological changes in the nucleus and mitochondria in macrophages infected with L. interrogans strain Lai (MOI 100) for 4 h. a: healthly cells showed normal cellular morphology, b: chromatin margination in leptospire-infected macrophages crescent, c: mitochondrial shape in healthly macrophages, d: disappearance of mitochondrial cristae in leptospire-infected macrophages. (D) The representative MMP changes in macrophages during infection with L. interrogans strain Lai for the indicated times determined by fluorescence microscopy. The red cells have a high MMP while the green cells have a low MMP. (E) The representative MMP changes in macrophages during infection with L. interrogans strain Lai for the indicated times determined by flow cytometry. The FL2 channel indicates high MMP (red) while the FL1 channel shows low MMP (green). (F) Statistical summary of MMP changes by flow cytometry in leptospire-infected macrophages. Statistical data from experiments such as shown in (E). Bars show the mean ± SD of three independent experiments. The values at “0” h show the MMP values before infection. Five thousand cells were analyzed in each specimen. *p < 0.05 vs. MMP value of the macrophages before infection. (G) CSA blockage of the apoptosis in macrophages infected with L. interrogans strain Lai. Bars show the mean ± SD of three independent experiments. Five thousand cells were analyzed in each specimen. *p < 0.05 vs. apoptotic ratios in each macrophage type unpretreated with CSA infected with the spirochetes.
Graphs and visual data illustrate apoptosis and mitochondrial membrane potential (MMP) loss in J774A.1 and THP-1 cells. Panels A and B show apoptosis at different multiplicities of infection (MOI) and over time. Panel C displays electron micrographs of infected cells. Panel D shows fluorescence images over time. Panel E presents flow cytometry scatter plots of JC-1-stained cells. Panel F graphs MMP loss, and Panel G compares apoptosis in leptospire-infected cells with and without CSA treatment, highlighting increases over time.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.