AUTHOR=Yu Jingxuan , Liu Zhenyu , Wang Yan , Zhou Yu , Liu Wei , Wang Tao , Xie Qiubo , Tian Hongzhe , Xu Yalong , Wang Min , Zhao Fuhan , Wang Lin , Zhang Guan , Chen Dongliang , Gao Lei , Pan Tiejun 

TITLE=Propionic acid mediates the renoprotective effects of fecal microbiota transplantation against ischemia–reperfusion injury via upregulating GPR43

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=Volume 15 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2025.1616164

DOI=10.3389/fcimb.2025.1616164

ISSN=2235-2988

ABSTRACT=IntroductionKidney ischemia–reperfusion injury (IRI) is a major cause of acute kidney injury (AKI), characterized by aggravated inflammation and apoptosis following reperfusion. This study aimed to investigate the protective effects and mechanisms of fecal microbiota transplantation (FMT) in a rat model of kidney IRI.MethodsSprague–Dawley rats(SDRs) subjected to 45 minutes of bilateral renal ischemia followed by reperfusion were prophylactically treated with FMT derived from guinea pigs or supplemented with propionic acid. Renal function, histopathology, inflammatory markers, apoptosis, proliferation, and gut microbiota composition were systematically evaluated.ResultsThe results demonstrated that FMT attenuated kidney IRI by remodeling the gut microbiota to enhance propionic acid production, which subsequently modulated inflammation and apoptosis via GPR43 signaling.ConclusionsThese findings provide novel insights into microbiota-targeted therapeutic strategies for kidney IRI and highlight propionic acid as a potential therapeutic agent.