Front. Cell. Infect. Microbiol. Frontiers in Cellular and Infection Microbiology Front. Cell. Infect. Microbiol. 2235-2988 Frontiers Media S.A. 10.3389/fcimb.2021.664366 Cellular and Infection Microbiology Original Research Cytospora and Diaporthe Species Associated With Hazelnut Canker and Dieback in Beijing, China Gao Hong Pan Meng Tian Chengming Fan Xinlei * The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing, China

Edited by: Hiran A. Ariyawansa, National Taiwan University, Taiwan

Reviewed by: Lukasz Kozubowski, Clemson University, United States; Asha Janadaree Dissanayake, University of Electronic Science and Technology of China, China

*Correspondence: Xinlei Fan, xinleifan@bjfu.edu.cn

This article was submitted to Fungal Pathogenesis, a section of the journal Frontiers in Cellular and Infection Microbiology

 02 08 2021 2021 11 664366 05 02 2021 16 04 2021 Copyright © 2021 Gao, Pan, Tian and Fan 2021 Gao, Pan, Tian and Fan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Hazelnut (Corylus heterophylla Fisch.) is an important nut crop in China but has been declining owing to the destructive effects of fungal branch canker and dieback. The identification and management of these pathogens are difficult because of the lack of attention to branch canker, insufficient understanding of phylogenetic, and overlapping morphological characteristics of the pathogens. In total, 51 strains were isolated from Chinese wild hazelnut in this study, and three species of Cytospora and two of Diaporthe were identified through morphological observation and multi-locus phylogenetic analyses (ITS, act, rpb2, tef1-α, and tub2 for Cytospora; ITS, cal, his3, tef1-α, and tub2 for Diaporthe). Three new species, Cytospora corylina, C. curvispora, and Diaporthe corylicola, and two known species, Cytospora leucostoma and Diaporthe eres, grew at 5–30°C and a pH of 3.0–11.0, with optimum growth at approximately 25°C and pH 4.0–7.0. Additionally, the effects of six carbon sources on mycelial growth were investigated. This study explored the main pathogenic fungi species of Corylus heterophylla, completed the corresponding database of pathogenic fungi information, and clarified their biological characteristics. Moreover, the results of this study provided a theoretical basis for Corylus heterophylla disease management and prevention in China.

 Ascomycota biological characterization Diaporthales mycelial growth phylogeny taxonomy Introduction

Branch canker and dieback are important forest diseases caused by fungal pathogens in the phylum Ascomycota, especially those in the genera Cytospora (Cytosporaceae, Diaporthales) and Diaporthe (Diaporthecae, Diaporthales) (Adams et al., 2005; Rossman et al., 2007; Gomes et al., 2013; Udayanga et al., 2015; Fan et al., 2020). In total, 672 species epithets of Cytospora have been recorded in the Index Fungorum (April 2021; www.indexfungorum.org/). Recent studies reported that approximately 150 species of Cytospora caused branch canker and dieback on more than 130 woody host species (Spielman, 1985; Adams et al., 2002; Adams et al., 2005; Kirk et al., 2008; Fan et al., 2014a; Fan et al., 2014b; Fan et al., 2020; Pan et al., 2020; Pan et al., 2021). More than 1,137 species epithets of Diaporthe have been enumerated in the Index Fungorum (April 2021; www.indexfungorum.org/), and over 200 species have been accepted recently (Udayanga et al., 2011; Dissanayake et al., 2017; Yang et al., 2020). These species are pathogenic, endophytic, or saprobic to a wide range of plant hosts (Adams et al., 2002; Adams et al., 2005; Udayanga et al., 2011; Fan et al., 2020). Some of these infect the stems, branches, twigs, and even roots of many commercial plants, and cause necrotic damage to young tissues, canker and dieback on branches, and, ultimately, the death of the host, resulting in serious economic losses (Fotouhifar et al., 2010; Udayanga et al., 2011; Udayanga et al., 2012a; Udayanga et al., 2012b; Guerrero and Pérez, 2013b). Symptoms of infected branches include fruiting bodies that immersed in the bark and erupted through the bark surface when mature; however, symptoms are not always the same (Santos et al., 2010; Jiang et al., 2020; Pan et al., 2020). Previous studies on species identification were conducted based on host affiliation and morphology, but different species can infect one host, and single species can infect multiple hosts (Adams et al., 2005; Yang et al., 2020; Zhu et al., 2020). Thus, accurate species identification requires polyphasic approaches based on ecology, additional morphological observations, and multi-locus phylogeny analyses (Harrington and Rizzo, 1999; Mostert et al., 2001; Adams et al., 2002; Adams et al., 2005; Udayanga et al., 2014a; Udayanga et al., 2014b; Du et al., 2016).

Hazelnut (Corylus spp.), a common tree that is extensively distributed in Asia, Europe, and North America, has important economic and nutritional value (Özdemir et al., 2001). Corylus heterophylla has been widely cultivated in China for centuries. More than four million acres of natural hazel grow in northern China with an annual yield of over 23,000 tons (Hu, 2016a). However, recently, global hazelnut production has declined because of the destructive effects of branch canker (Linaldeddu et al., 2016). Eastern filbert blight, one of the most destructive diseases of Corylus americana, was caused by Anisogramma anomala, which became commercially important in the 1970s (Gottwald and Cameron, 1980; Pinkerton et al., 1992; Chen et al., 2007). Cytospora corylicola has been recognized as a rot agent in European hazelnuts in Italy (Servazzi, 1950; Graniti, 1957; Salerno, 1961; Guerrero and Pérez, 2013a; Guerrero and Pérez, 2013b). Several fungal pathogens have been reported to cause canker and dieback of Corylus avellana, including Anthostoma (Diatrypaceae), Diaporthe (Diaporthaceae), Diaporthella (Gnomoniaceae), Diplodia, Dothiorella (Botryosphaeriaceae), and Gnomoniopsis (Gnomoniaceae) (Guerrero and Pérez, 2013a; Guerrero and Pérez, 2013b; Linaldeddu et al., 2016; Wiman et al., 2019). However, these studies mainly focused on canker and dieback of European hazelnuts, and only the genera Erysiphe and Trichothecium have been recorded in China (Sun, 2013; Hu, 2016b). Cytospora coryli and Diaporthe coryli, pathogens of Corylus mandshurica, have been collected from Beijing and Shaanxi Province, and are of great significance for research on wild hazelnuts in China (Yang et al., 2020; Zhu et al., 2020). As Corylus heterophylla is the main source of hazel products in the Chinese market (Liu et al., 2014a; Liu et al., 2014b), and given the importance of Corylus species in the Chinese economy, the fungal pathogens associated with canker and dieback of C. heterophylla need to be investigated.

During an investigation of cognitive practices in Beijing, China, 51 strains were isolated from the symptomatic stems and branches of Corylus heterophylla. The purpose of this study was to identify these strains using polyphasic approaches and supplement a multi-locus DNA dataset of the pathogens of Chinese wild hazelnut. Additionally, the influences of temperature, pH, and six carbon sources on mycelial growth were determined to evaluate the possible role of these conditions in fungal growth.

 Materials and Methods Sampling and Isolation

During the investigation of cognitive practices (June to August, 2019), more than 70 specimens were collected from stems and branches of Corylus heterophylla in Huairou District of Beijing, China. These infected stems and branches were collected from three nurseries, expressed typical canker and dieback symptoms with fruiting bodies immersed and erupted through the bark surface when mature ( Figure 1 ). Twenty-five specimens were selected and taken to laboratory, observed using a stereo microscope (M205 FA) (Leica microsystem, Wetzlar, Genmany). A total of 51 strains were established by transferring the ascospores or conidial masses from the fruiting bodies on to the surface of PDA (1.8% potato dextrose agar, potato 20 g, dextrose 20 g, agar 17 g, distilled water to complete 1,000 ml) plates with diameter 90 mm. The strains were incubated in darkness at 25°C for 24 h until spores germinated. Single germinating spores were moved to new PDA plates. Specimens have been maintained at the Museum of the Beijing Forestry University (BJFC) and the working Collection of X.L. Fan (CF), housed at the Beijing Forestry University. Living cultures are deposited in the China Forestry Culture Collection Centre (CFCC).

Disease symptoms of Corylus heterophylla. (A–C) Death of the hazel trees caused by Cytospora and Diaporthe in the orchards. (D–F) Conidiomata on a naturally infected stem in the field.

 Morphological Observations

Species identification was based on morphological features of the fruiting bodies, which was produced on the infected plant tissues, including stromata (arrangement and size), conceptacle (presence or absence), conidiomata (color, size, and shape), ostioles (number and diameter), locules (number and arrangement type), conidiophores, and conidia (size and shape), supplemented by cultural characteristics such as colony color, texture, and the presence or absence of airborne hyphae (Mostert et al., 2001; Zhang et al., 2007; Zhu et al., 2020). The morphological features were observed under a Leica stereomicroscope (M205 FA) (Leica microsystem, Wetzlar, Genmany). Micro-morphological observations determined by a Nikon Eclipse 80i compound microscope. Measuring 30 conidiomata/ascomata and 50 conidia/ascospores, determined by length, width, and length/width ratio (L/W ratio). Recording the colony diameters and describing the color was based on the color charts of Rayner (1970) after 1–2 weeks on PDA in darkness. The results were edited manually by Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5.

 DNA Extraction and PCR Amplification

Scrapping the mycelium from the cellophane for DNA extraction after three days dark-incubation at 25°C. Using the modified CTAB method to extract genomic DNA (Doyle and Doyle, 1990). Using a 20 μl system of 10 μl Mix (Promega), 7 μl ddH2O, 1 μl upstream primer, 1 μl downstream primer, and 1 μl template DNA to conduct polymerase chain reaction (PCR) in order to amplify gene fragments. The primers and PCR conditions are set in Table S1 . Electrophoretic separation was conducted for the PCR amplification products in 2% agarose gels with a DNA maker 2,000 bp (Takara Biotech). Using an ABI PRISM® 3730XL DNA Analyzer with BigDye® Terminater Kit v. 3.1 (Invitrogen) at the Shanghai Invitrogen Biological Technology Company (Beijing, China) to conduct the DNA sequences. In order to acquire a consensus sequence of sequences obtained from forward and reverse primer pairs, Seqman v. 9.0.4 (DNASTAR Inc., Madison, WI, United States) was used.

 Phylogenetic Analyses

Using MAFFT v. 6 (Katoh and Standley, 2013) to align ITS sequence data and editing it manually with MEGA v. 6.0 (Tamura et al., 2013), the current strains were preliminarily identified as Cytospora and Diaporthe species. To clarify their further phylogenetic position, five genes (ITS, act, rpb2, tef1-α, and tub2 for Cytospora; ITS, cal, his3, tef1-α, and tub2 for Diaporthe) for each genus were combined and aligned to compare with other strains in GenBank secondly. Generating subsequent alignments for each gene and adjusting them manually. Excluding the ambiguously aligned sequences from analyses. Reference sequences were retrieved from recent publications (Fan et al., 2014a; Fan et al., 2014b; Fan et al., 2015a; Fan et al., 2015b; Lawrence et al., 2017; Fan et al., 2020; Pan et al., 2020; Zhu et al., 2020; Pan et al., 2021). Diaporthe vaccinii (CBS 160.32) was included as the outgroup in Cytospora analysis ( Table S2 ) and Diaporthella corylina (CBS 121124) was included in Diaporthe analysis ( Table S3 ). All the datasets were performed using PAUP v. 4.0b10 for the maximum parsimony (MP) method (Swofford, 2003), RAxML for the maximum likelihood (ML) method (Stamatakis, 2006), and MrBayes v. 3.1.2 for the Bayesian Inference (BI) method (Ronquist and Huelsenbeck, 2003) for the phylogenetic analyses. All novel sequences derived from this study were deposited in MycoBank (www.mycobank.org) (Crous et al., 2004). All sequences from this study were submitted in GenBank, as shown in Tables S2 and S3 . The multi-gene sequence alignment files were deposited in TreeBASE (www.treebase.org; accession number: S276989).

 Testing the Influence of Temperature, pH, and Carbon on Mycelium Growth

All 51 strains we collected were identified as three species of Cytospora and two of Diaporthe through morphological and phylogenetic analyses. We selected representative strains of each species to assess the influences of temperature, pH, and carbon sources on mycelium growth incubated in the dark. A mycelial plug with 5-mm-diameter was transferred on to the center of a 90 mm PDA plate and incubated in an environment of 0–40°C with a 5°C gradient (i.e., 0, 5, 10, 15, 20, 25, 30, 35, and 40°C), three repeats were set for each treatment (Fang, 1979; Zhou et al., 2020). The pH values of the PDA were regulated to a range of 2.0–12.0 using 1 mol/L NaOH and 1 mol/L HCl, to obtain pH 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, 11.0, and 12.0. Taking a mycelial plug to PDA plate with the same methods as temperature tests and incubating in darkness at 25°C, three replicates were also set. The strains of the five species were incubated in darkness at 25°C on PDA (the 20 g dextrose were replaced by 20 g fructose, galactose, maltose, sucrose, or xylose) plates to assess the utilization of these compounds as carbon sources (Zhao et al., 2019; Zhou et al., 2020). The colony diameter was measured in millimeters every 24 h for four days of incubation, and converting the data to radial growth to assess the effects of temperature, pH, and carbon sources on mycelial growth (Zhao et al., 2019). All data were analyzed with one-way ANOVA and Post Hoc of LSD and Tukey using IBM SPSS Statistics v. 22.0 (IBM Inc., Armonk, NY, USA). A p-value < 0.05 was considered significant. Graph with SE-bar were conducted in order to explain the difference in mycelium growth under different conditions.

 Results Isolation

During the investigation of cognitive practices, we isolated a total of 51 strains from infected stems and branches of hazelnut trees ( Table S4 ). Among the 51 strains, 14 strains were identified as Cytospora species (37%) and 37 strains were Diaporthe (73%). Diaporthe corylicola is the main species observed on Corylus heterophylla (64.71%), followed by Cytospora curvispora (11.76%). The rest were identified as Cytospora corylina, Cytospora leucostoma, and Diaporthe eres, four strains of each species, accounting 7.84% of total, respectively.

 Phylogeny

Totally 14 strains of Cytospora obtained from hazelnut and other 224 strains retrieved from recent publications were used in the phylogenetic analyses (Spielman, 1985; Adams and Taylor, 1993; Adams et al., 2002; Adams et al., 2005; Lawrence et al., 2017; Fan et al., 2018; Fan et al., 2020; Jiang et al., 2020; Pan et al., 2020; Zhou et al., 2020; Zhu et al., 2020; Pan et al., 2021). The sequence datasets for the five genes (ITS, act, rpb2, tef1-α, and tub2) were performed in individual and combined analyses. The single gene region analysis is very similar to the tree topologies of the combined analyses. The phylogram generated here indicated 237 ingroup taxa including 3,686 characters (622 for ITS, 457 for act, 1,076 for rpb2, 775 for tef1-α, and 756 for tub2) in the multi-locus analyses, of which 2,010 characters were constant, 191 variable characters were parsimony uninformative and 1,485 characters were parsimony informative. MP analysis generated 200 parsimonious trees (TL = 9,878, CI = 0.307, RI = 0.812, RC = 0.249) and the first one is selected and shown in Figure 2 . All trees of ML and BI analyses were in agreement and no significant difference with MP tree. MP/ML-BS (MP/ML bootstrap support) ≥50% were shown, and branches with BPP (BI posterior probability) ≥0.95 were thickened in the phylogram.

Phylogram of Cytospora based on combined five genes (ITS, act, rpb2, tef1-α, and tub2). MP/ML-BS ≥50% are shown at first/second positions. Branches with BPP ≥0.95 are thickened. Strains in this study are in blue. Ex-type strains are in bold.

Thirty-seven strains of Diaporthe from hazelnut were aligned with 253 strains supplement from recent publications. The five genes (ITS, cal, his3, tef1-α, and tub2) in individual and combined were used to clarify the phylogenetic position of these Diaporthe species. The tree topologies generated by the MP, ML, and BI analyses were similar. All strains counted 2,983 characters including gaps (613 for ITS, 587 for cal, 578 for his3, 645 for tef1-α, and 560 for tub2), of which 1,233 characters are constant, 360 variable characters are parsimony uninformative 1,390 characters are parsimony informative. MP analysis generated 100 parsimonious trees (TL = 1,826, CI = 0.267, RI = 0.788, RC = 0.210), the first one is selected and presented in Figure 3 . The support values were shown and branches were thickened in the phylogram at the same standard as Figure 2 .

Phylogram of Diaporthe based on combined five genes (ITS, cal, his3, tef1-α, and tub2). MP/ML-BS ≥50% are shown at first/second positions. Branches with BPP ≥0.95 are thickened. Strains in this study are in blue. Ex-type strains are in bold.

The current 14 strains of Cytospora clustered in three clades representing three species, of which one was known as C. leucostoma and two were distinguished from all other known taxa with high support values (MP/ML/BI = 100/100/1) as two clades. Thus, we described the two clades as C. corylina, C. curvispora here. Four strains were assigned to Diaporthe eres, and the remaining 33 were classified as one new species, D. corylicola, representing a monophyletic clade with high support values (MP/ML/BI = 100/100/1).

 Taxonomy

Cytospora corylina H. Gao & X.L. Fan, sp. nov. Figure 4 .

Cytospora corylina from Corylus heterophylla. (A, B) Habit of conidiomata on twig. (C) Transverse section of conidioma. (D) Longitudinal section through conidioma. (E, F) Conidiophores and conidiogenous cells. (G) Conidia. Scale bars: 3 mm (A); 200 µm (B–D); 10 µm (E–G).

MycoBank MB 838643.

Holotype: CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26′16.66″E, 40°52′52.18″N, from branches of Corylus heterophylla, June, 2019, H. Gao & X.L. Fan (holotype BJFC CF20210114), ex-type living culture CFCC 54684.

Etymology: Named after the host genus on which it was collected, Corylus.

Description: Necrotic tissues on branches of Corylus heterophylla. Sexual morph: not observed. Asexual morph: Stromata immersed in bark. Pycnidial stromata ostiolated, scattered, erupted slightly through the bark surface. Multiple locules, conceptacle absent. 850–1,280 (av. = 913, n = 30) µm in diameter. Ectostromatic disc gray with one ostiole in center, discoid, circular to ovoid, 180–270 (av. = 214, n = 30) µm in diameter. Ostiole slight-brown, inconspicuous, at the same level as the disc surface. 110–185 (av. = 135, n = 30) µm in diameter. Locules numerous, circular to irregular with common walls generally invaginated. Conidiophores branched, occasionally unbranched, hyaline, approximately cylindrical, 8.0–13.5 × 1.0–2.5 (av. = 10.8 ± 1.6 × 1.6 ± 0.4, n = 30) µm, sometimes reduced to conidiogenous cells. Conidiogenous cells enteroblastic, phialidic, subcylindrical to cylindrical. Conidia hyaline, allantoid, smooth, aseptate, thin-walled, 3.5–7.5 × 1.0–1.5 (av. = 5.8 ± 0.8 × 1.3 ± 0.2, n = 50) µm, L/W ratio 3.61–4.52 (av. = 4.27 ± 0.17).

Culture characteristics: Colonies are initially white, reaching 90 mm after two days, slightly fawn in center and becoming nearly fucous black after 30 days. Colonies concentric circles, with a thick texture, and aerial mycelium lacked. Conidiomata distributed on PDA surface irregularly surrounded by dark mycelium ( Figure 9A ).

Other specimens examined: CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26′21.62″E, 40°52′47.58″N, from branches of Corylus heterophylla, June, 2019, H. Gao & X.L. Fan (BJFC C20210115), living culture CFCC 54685 and CFCC 54686; ibid. BJFC CF20210116, living culture CFCC 54687.

Notes: Cytospora corylina was collected from Corylus heterophylla in Beijing, China. It is significantly different from Cytospora coryli isolated by Zhu et al. (2020) in morphology. Cytospora coryli has a flat conidiomata and inconspicuous ostiole. In terms of culture morphology, The colonies of C. coryli are brown, conidiomata distributed radially on colony surface. The four strains representing Cytospora corylina clustered as a single lineage and appeared to be the most closely related to C. gigaspora, C. mali-spectabilis, C. nivea, C. paratranslucens, and C. translucens, with support values of MP/ML/BI = 57/76/0.99. However, this strain could be distinguished from the five most related species by the conidiomata (C. gigaspora with a flat locule, C. mali-spectabilis with a column lenticular tissue in the center, and C. nivea with a dark conceptacle) (Fan et al., 2015b; Pan et al., 2020). In addition, C. corylina has a smaller conidia size (3.5–7.5 × 1–1.7 µm) than C. gigaspora (8.9–12.1 × 1.9–2.9 µm), C. mali-spectabilis (9.0–10.0 × 1.5–2 µm), C. nivea (6.2–9.2 × 1.7–2.4 µm), and C. paratranslucens (6.5–7.3 × 1.3–1.5 µm) (Adams et al., 2006; Fan et al., 2015b; Norphanphoun et al., 2017; Pan et al., 2020). Therefore, we describe this species as novel based on DNA sequence data and morphology.

Cytospora curvispora H. Gao & X.L. Fan, sp. nov. Figure 5 .

Cytospora curvispora from Corylus heterophylla. (A, B) Habit of conidiomata on twig. (C) Transverse section of conidioma. (D) Longitudinal section through conidioma. (E) Conidiophores and conidiogenous cells. (F) Conidia. Scale bars: 2 mm (A); 500 µm (B–D); 10 µm (E, F).

MycoBank MB 838641.

Holotype: CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26'23.64"E, 40°52'48.37"N, from branches of Corylus heterophylla, June, 2019, H. Gao & X.L. Fan (holotype BJFC CF20210110), ex-type living culture CFCC 54000.

Etymology: Named after the character of its curved conidia.

Description: Necrotic tissues on stems and branches of Corylus heterophylla. Sexual morph: not observed. Asexual morph: Stromata immersed in bark. Conidiomata pycnidial, scattered, conical, erupted through the bark surface when mature, locules multiple. Conceptacle absent, diameter 1,080–1,700 (av. = 1,423, n = 30) µm. Ectostromatic disc black-brown, discoid, circular to ovoid, 480–660 (av. = 526, n = 30) µm in diameter, with one ostiole. Ostiole dark-gray to black, inconspicuous, slightly curved, at the same level as the disc surface, 40–70 (av. = 58, n = 30) µm in diameter. Locules numerous, circular to irregular with common wall generally invaginated. Conidiophores unbranched, barely branched, hyaline, approximately cylindrical, 9.5–14.0 × 1.0–1.5 (av. = 12.5 ± 1.3 × 1.1 ± 0.3, n = 30) µm, sometimes reduced to conidiogenous cells. Conidiogenous cells enteroblastic, phialidic, subcylindrical to cylindrical. Conidia hyaline, elongate­allantoid to falcate, smooth, aseptate, thin-walled, 4.5–8.5 × 1.0–1.5 (av. = 6.7 ± 1.0 × 1.3 ± 0.2, n = 50) µm, L/W ratio 4.88–6.04 (av. = 5.19 ± 0.22).

Culture characteristics: Cultures are initially white, thin, reaching 70 mm after three days, turning slightly honey after 30 days and deepened continuously. Colonies are uniform, aerial mycelium lacked. Conidiomata distributed irregularly on PDA surface ( Figure 9B ).

Cultures on potato dextrose agar (PDA). (A) Cytospora corylina. (B) Cytospora curvispora. (C) Cytospora leucostoma. (D, E) Diaporthe corylicola. (F) Diaporthe eres. Days of incubation: three days at left and 30 days at right (A–C), (F); three days at left and seven days at right (D); 15 days at left and 30 days at right (E).

Other specimens examined: CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26'28.24"E, 40°52'48.73"N, from branches of Corylus heterophylla, June, 2019, H. Gao & X.L. Fan (BJFC CF20210111), living culture CFCC 54001; CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26'22.83"E, 40°52'50.57"N, from branches of C. heterophylla, June, 2019, H. Gao & X.L. Fan (BJFC CF20210118), living culture CFCC 54676 and CFCC 54677; CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26'20.22"E, 40°52'41.15"N, from stems of C. heterophylla, June, 2019, H. Gao & X.L. Fan (BJFC CF20210113), living culture CFCC 54678 and CFCC 54679.

Notes: Cytospora curvispora was isolated from Corylus heterophylla in Beijing, China. We can distinguish it from C. coryli by its curved conidia and conical conidiomata. In the phylogeny analysis, Cytospora curvispora clustered with C. elaeagnicola, C. spiraeae, and C. spiraeicola with support values of MP/ML/BI = 84/88/0.99. However, differences in their distribution and morphology were identified. Cytospora spiraeae and C. spiraeicola were isolated from Spiraea salicifolia, collected from Gansu Province and Beijing, respectively. Cytospora elaeagnicola was collected from branches of Elaeagnus angustifolia in the Xinjiang Uygur Autonomous Region. Morphologically, the conidia of C. curvispora are more curved and with a smaller width (1.0–1.5 µm for C. curvispora, 1.5–2.0 µm for C. elaeagnicola, 2.0–2.5 µm for C. spiraeae, 2.5–3.5 µm for C. spiraeicola). In addition, C. elaeagnicola colonies are white and have a thick texture at the center, becoming thinner at the edges; C. spiraeae is fawn and felt-like and C. spiraeicola is buff to hazel with a heterogeneous texture (Zhu et al., 2018; Zhang et al., 2019; Zhu et al., 2020). Thus, we described this finding as novel based on morphological and phylogenetic analyses.

Cytospora leucostoma (Pers.) Sacc., Michelia, 2: 264, 1881. Figure 6 .

Cytospora leucostoma from Corylus heterophylla. (A) Habit of ascomata on twig. (B) Transverse section of ascomata. (C) Longitudinal section through ascomata. Scale bars: 2 mm (A); 1 mm (B, C).

Description: see Fan et al. (2020).

Culture characteristics: Colonies are white initially and change to dark green to dark after seven days. Growing up to 80 mm after three days. Colonies have a uniform texture, thick, aerial mycelium lacked ( Figure 9C ).

Specimens examined: CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26′15.67″E, 40°52′40.18″N, from branches of Corylus heterophylla, June, 2019, H. Gao & X.L. Fan (BJFC CF20210117), living culture CFCC 54680 and CFCC 54681; CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26′36.65″E, 40°52′32.14″N, from branches of C. heterophylla, June, 2019, H. Gao & X.L. Fan (BJFC CF2021118), living culture CFCC 54682 and CFCC 54683.

Notes: Cytospora leucostoma commonly causes branch canker in Rosaceae in China (Fan et al., 2020; Pan et al., 2020). In the current study, two specimens were collected from the infected branches of the Chinese hazelnut, and fruiting bodies were observed. However, the specimens were not fresh, and little information is available on the micromorphology. Thus, we identified the strains as C. leucostoma, based on ascostromata with conceptacle, culture characteristics, and DNA data.

Diaporthe corylicola H. Gao & X.L. Fan, sp. nov. Figure 7 .

Diaporthe corylicola from Corylus heterophylla. (A, B) Habit of conidiomata on twig. (C) Transverse section of conidiomata. (D) Longitudinal section through conidioma. (E, F) Conidiogenous cells and conidia. (G, H) Alpha and gamma conidia. Scale bars: 3 mm (A); 500 µm (B–D); 10 µm (E–H).

MycoBank MB 838644.

Holotype: CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26′18.55″E, 40°52′38.27″N, from stems of Corylus heterophylla, June, 2019, H. Gao & X.L. Fan (holotype BJFC CF20210121), ex-type living culture CFCC 53986.

Etymology: Named after the host genus on which it was collected, Corylus.

Description: Necrotic tissues on stems and branches of Corylus heterophylla. Asexual morph: Stromata immersed in bark. Pycnidial stromata ostiolated, scattered, discoid to conical, erumpent slightly through the surface of bark at maturity, with single locule. Conceptacle absent, 750–1,300 (av. = 991, n = 30) µm in diameter. Ectostromatic disc buff or orange with only one ostiole in center, covered by orange discharged conidial masses, discoid, circular to ovoid, 175–270 (av. = 206, n = 30) µm in diameter. Ostiole dark-brown, conspicuous, at the same level as the disc surface, 130–150 (av. = 141, n = 30) µm in diameter. Conidiogenous cells enteroblastic, phialidic, subcylindrical to cylindrical, 16.0–24.0 × 1.5–2.5 (av. = 19.4 ± 2.5 × 2.0 ± 0.2, n = 30) µm. Alpha conidia are aseptate, hyaline, fusiform, multi-guttulate, rarely 2 guttulate and smooth, 11.0–16.5 × 2.0–3.5 (av. = 13.8 ± 1.3 × 2.8 ± 0.3, n = 50) µm, L/W ratio 4.58–5.41 (av. = 4.87 ± 0.19). Gamma conidia hyaline, multi-guttulate, subcylindrical with a nearly rounded apex, 13.0–19.5 × 1.5–2.5 (15.3 ± 1.1 × 1.7 ± 0.2, n = 50) µm, L/W ratio 6.68–12.61 (av. = 8.92 ± 1.28). Beta conidia undetermined.

Culture characteristics: Colonies are white initially, only 25 mm after three days and going to buff after 15 days. Colonies are felty with thick texture, aerial mycelium lacked and conidiomata are randomly distributed fat the marginal area, with orange conidial drops oozing out of the ostioles ( Figures 9D, E ).

Other specimens examined: CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26′13.81″E, 40°52′58.11″N, from stems of Corylus heterophylla, June, 2019, H. Gao & X.L. Fan (BJFC CF20210122), living culture CFCC 53987; ibid. BJFC CF20210123, living culture CFCC 53988 to 53990; BJFC CF20210134, living culture CFCC 54944 and CFCC 54945; CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26′25.25″E, 40°52′29.83″N, from stems of C. heterophylla, June, 2019, H. Gao & X.L. Fan (BJFC CF20210124), living culture CFCC 53991 to CFCC 53993; ibid. BJFC CF20210125, living culture CFCC 53994 and CFCC 54704; BJFC CF20210126, living culture CFCC 53995; CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26′21.21″E, 40°52′38.02″N, from branches of C. heterophylla, June, 2019, H. Gao & X.L. Fan (BJFC CF20210127), living culture CFCC 53996 to 53998; ibid. BJFC CF20210128, living culture CFCC 554696 and CFCC 54697; BJFC CF20210129, living culture CFCC 54698 to CFCC 54701; BJFC CF20210130, living culture CFCC 54702 to CFCC 54705; CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26′28.67″E, 40°52′36.72″N, from stems of C. heterophylla, June, 2019, H. Gao & X.L. Fan (BJFC CF20210131), living culture CFCC 54706 to CFCC 54708; ibid. BJFC CF20210132, living culture CFCC 54709 to CFCC 54712; BJFC CF20210133, living culture CFCC 54713.

Notes: Diaporthe corylicola was isolated from Corylus heterophylla in Beijing, China. The cluster represented a single lineage with high support (MP/ML/BI = 100/100/1). The culture morphology was similar to that of D. coryli, which was isolated from Corylus mandshurica, but alpha conidia were longer and thinner (11.0–16.5 × 2.0–3.5 vs. 11.5–13 × 3–3.5 µm) (Yang et al., 2020). Phylogenetically, there was high contrast between Diaporthe coryli and D. corylicola, with 36/610 for ITS, 77/584 for cal, 63/575 for his3, 61/642 for tef1-α, and 61/556 for tub2. Thus, we describe this species as novel.

Diaporthe eres Nitschke, Pyrenomyc. Germ. 2: 245, 1870. Figure 8 .

Diaprthe eres from Corylus heterophylla. (A, B) Habit of conidiomata on twig. (C) Transverse section of conidioma. (D) Longitudinal section through conidioma. (E) Conidiogenous cells and conidia. (F) Alpha and beta conidia. Scale bars: 2 mm (A); 500 µm (B–D); 10 µm (E, F).

Synonyms are listed in Yang et al. (2018).

Description: Necrotic tissues on stems and branches of Corylus heterophylla. Sexual morph: not observed. Asexual morph: Stromata immersed in bark. Pycnidial stromata ostiolated, scattered or serried, discoid to conical, erumpent slightly through the bark surface at maturity, with single locule. Conceptacle absent. 140–380 (av. = 221, n = 30) µm in diameter. Ectostromatic disc brown to black with only one ostiole in center, discoid, circular to ovoid, 120–270 (av. = 163, n = 30) µm in diameter. Ostiole dark-grey, conspicuous, at the same level as the disc surface, 20–80 (av. = 56, n = 30) µm in diameter. Conidiogenous cells phialidic, cylindrical, terminal, 7.5–16.0 × 2.0–3.0 (av. = 12.2 ± 2.5 × 2.6 ± 0.3, n = 30) mm. Alpha conidia hyaline, aseptate, ellipsoidal, one guttulate at each end, 5.5–8.5 × 1.5–2.5 (av. = 6.5 ± 0.6 × 2.1 ± 0.2, n = 50) μm, L/W ratio, 2.58–4.75 (av. = 3.14 ± 0.41). Beta conidia hyaline, lanceolate to linear, 12.5–30.5 × 1.0–1.5 (av. = 23.9 ± 4.0 × 1.2 ± 0.2, n = 50) μm, L/W ratio 8.99–32.06 (av. = 19.91 ± 4.23).

Culture characteristics: Colonies with felty aerial mycelium, white, changing to compact at center later and sparse at surrounding. Growing up to 45 mm after three days incubation. Conidiomata sparse, black, distributed irregularly ( Figure 9F ).

Specimens examined: CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26′35.53″E, 40°52′47.18″N, from branches of Corylus heterophylla, June, 2019, H. Gao & X.L. Fan (BJFC CF20210119), living culture CFCC 53999 and CFCC 54714; CHINA, Beijing City, Huairou District, Forestry Center of Beijing University of Agriculture, 116°26′41.03″E, 40°52′29.63″N, from stems of C. heterophylla, June, 2019, H. Gao & X.L. Fan (BJFC CF20210120), living culture CFCC 54715 to 54716.

Notes: Diaporthe eres were defined first by Nitschke (1870), collected from Ulmus sp. in Germany. Udayanga et al. (2014b) described them as a complex and provided a phylogram of seven genes. Phenotypic plasticity and host affiliations have been used for species identification of this complex, but have shown little significance (Udayanga et al., 2014a; Udayanga et al., 2014b; Udayanga et al., 2015; Du et al., 2016; Gao et al., 2016; Gao et al., 2017; Dissanayake et al., 2017). Fan et al. (2018) investigated this complex using a three (cal, tef1-α, and tub2) data matrix and further identified the species. Diaporthe eres were identified as pathogens on hazel trees in Oregon (Battilani et al., 2018). The current results indicate four strains of D. eres from hazel trees in China.

 Effects of Temperature, pH, and Carbon Source on Mycelial Growth Effects on Mycelial Growth of <italic>Cytospora corylina</italic>

Cytospora corylina (CFCC 54684) expressed high adaptability to the three conditions and fast growth rate. Colonies grew from 5 to 30°C but not at 0, 35, or 40°C after 96 h of incubation ( Figure 10A ). The maximal growth occurred at 25°C after 24 h, at which point colonies grew up to 45 mm diameter, reaching 90 mm after 48 h. After 72 h, colonies reached 90 mm at 20°C and 70 mm at 30°C, and after 96 h, the colony diameter reached 81 mm. Colonies grew on PDA in the pH range 3.0–11.0 but not at 2.0 or 12.0 ( Figure 10B ). Mycelium had the highest growth rate at pH 6.0, reaching diameters of 25 mm after 24 h and 90 mm after 72 h, followed by pH 4.0 and 8.0, which resulted in colonies of 75 mm and 51 mm diameters, respectively, after 72 h. However, on the fourth day, colonies showed fast growth in pH 5.0, reaching up to 90 mm diameter. The slowest growth occurred at pH 11.0, with colonies reaching only seven millimeters in diameter after four days of incubation. In general, C. corylina is more suitable for weakly acidic conditions ranging from pH 4.0 to 7.0.

Cytospora corylina grew on all six tested carbon sources ( Figure 10C ). The mycelia grew faster in dextrose- and fructose-supplemented media after 24 h than in media supplemented with the other carbon sources. After 48 h, mycelium reached 90 mm diameter on dextrose-supplemented medium, followed by fructose-supplemented medium; maltose-supplemented medium showed the least efficiency in terms of growth. The difference in the utilization of sucrose and other carbon sources gradually became apparent after 72 h: growth in sucrose-supplemented medium was significantly lower than that in media supplemented with the other carbon sources, all of which resulted in mycelium reaching 90 mm diameter after 96 h.

 Effects on Mycelial Growth of <italic>Cytospora curvispora</italic>

Colonies of Cytospora curvispora (CFCC 54000) in the current study grew from 5 to 30°C but not at 0, 35, or 40°C after four days of dark incubation ( Figure 10D ). We observed the maximum total growth at 25°C after 24 h, and colonies grew up to 29 mm, followed by growth at 20 and 30°C. After 72 h, colonies reached 73 mm at 20 and 25°C and showed colony diameters of 90 mm after 96 h. Cytospora curvispora was highly adaptable to pH, the colonies of which could grow at pH 3.0–11.0 but not at 2.0 or 12.0 ( Figure 10E ). Growth was the fastest at pH 6.0, where mycelium reached 48 mm after 48 h and 90 mm after 96 h, followed by pH 7.0 in which a colony diameter of 78 mm was achieved. After 96 h, mycelial growth was the slowest at pH 11.0, reaching only 27 mm in diameter after four days of incubation.

(A) Effects of temperature on growth of Cytospora corylina. (B) Effects of pH on growth of Cytospora corylina. (C) Effects of carbon source on growth of Cytospora corylina. (D) Effects of temperature on growth of Cytospora curvispora. (E) Effects of pH on growth of Cytospora curvispora. (F) Effects of carbon source on growth of Cytospora curvispora. (G) Effects of temperature on growth of Cytospora leucostoma. (H) Effects of pH on growth of Cytospora leucostoma. (I) Effects of carbon source on growth of Cytospora leucostoma. Bars represent ± SE. Mycelium length not connected by the same letter in a group are apparently different (p <0.05) for that condition.

Cytospora curvispora could utilize all six carbon sources ( Figure 10F ). After 24 h, the utility of the six different carbon sources was almost the same, except for sucrose. However, the difference in the utilization of these carbon sources gradually became apparent after 48 h. Growth on xylose- and fructose-supplemented media were significantly greater than that on the other carbon source-supplemented media, showing no difference after 96 h, with colonies of 90 mm diameter.

 Effects on Mycelial Growth of <italic>Cytospora leucostoma</italic>

Cytospora leucostoma (CFCC 54680) expressed high adaptability to the three conditions. Colonies of it in the current study grew from 5–30°C but not at 0, 35, or 40°C after four days of incubation ( Figure 10G ). The maximum growth rate was observed at 25°C. Colonies reached 90 mm diameter at 25 and 30°C and nearly 80 mm in diameter after 72 h. At 20, 25, and 30°C, mycelium grew up to 90 mm after 96 h. Colonies could grow at a pH range of 3.0–11.0 but not at 2.0 and 12.0 ( Figure 10H ). We observed the maximum growth rate at pH 6.0, with diameters reaching 35 mm after 24 h and 72 mm after 48 h, followed by pH 5.0 and 7.0, both of which resulted in diameters of 45 mm after 48 h. After 96 h, colonies grew up to 90 mm diameter under pH 4.0, 5.0, 6.0, and 7.0 and reached 88 mm at pH 8.0.

Cytospora leucostoma could utilize six carbon sources ( Figure 10I ). The utility of dextrose, galactose, and fructose was greater than that of sucrose, xylose, and maltose after 96 h of incubation. After 72 h, mycelia growing on dextrose- and galactose-supplemented media were the first to reach 90 mm diameter, followed by colonies growing on fructose- and sucrose-supplemented media. Colonies grew slower on maltose-supplemented medium, reaching only 63 mm after four days.

 Effects on Mycelial Growth of <italic>Diaporthe corylicola</italic>

Colonies of Diaporthe corylicola (CFCC 53986) in the current study grew on 10–30°C ( Figure 11A ). The maximum growth rate occurred at 25°C, but almost no growth was observed on the first day. Diaporthe corylicola grew slowly, and the colony diameter only reached 35 mm at 25°C after 96 h, followed by 18 mm diameter at 20°C. Colonies grew on PDA in the pH range of 3.0–11.0 but not at 2.0 or 12.0 ( Figure 11B ). The maximum mycelial growth was achieved at pH 6.0, reaching 35 mm after 96 h, followed by pH 5.0 and 4.0, and growth was slower at the other pH conditions. All six carbon sources tested could be metabolized by D. corylicola ( Figure 11C ). In the first three days, no significant difference was observed among the growth of colonies in media supplemented with all carbon sources, except sucrose. After 96 h of incubation, the utilization of dextrose and galactose was apparently greater, while that of the other four carbon sources reached nearly the same level.

(A) Effects of temperature on growth of Diaporthe corylicola. (B) Effects of pH on growth of Diaporthe corylicola. (C) Effects of carbon source on growth of Diaporthe corylicola. (D) Effects of temperature on growth of Diaporthe eres. (E) Effects of pH on growth of Diaporthe eres. (F) Effects of carbon source on growth of Diaporthe eres. Bars represent ± SE. Mycelium length not connected by the same letter in a group are apparently different (p <0.05) for that condition.

 Effects on Mycelial Growth of <italic>Diaporthe eres</italic>

Colonies of Diaporthe eres (CFCC 53999) grew at 5–30°C but not at 0°C, 35°C, or 40°C after four days. At 25°C, the maximum growth rate was achieved at 24 h, while at 30°C, the maximum growth rate was achieved at 96 h ( Figure 11D ). Colony growth was comparable at 20–30°C. After 96 h, colonies reached 65 mm diameter at 30°C, followed by 61 mm and 56 mm, respectively, at 25 and 20°C, while at the other temperatures, a slow growth rate was observed with no more than 25 mm diameter. Diaporthe eres were highly adaptable to pH, the colonies of which grew on PDA at a pH range of 3.0–11.0 but not at 2.0 or 12.0 ( Figure 11E ). Mycelium grew the fastest at pH 6.0, reaching 61 mm after 96 h, followed by pH 7.0, reaching 43 mm diameter after 96 h. In the other pH environments, although the growth rate was quite different from that at pH 6.0 and 7.0, it was maintained at a relatively high level.

All six carbon sources were utilized by D. eres ( Figure 11F ). After 24 h, the mycelial length was the same for colonies grown on media containing the six different carbon sources and was maintained at a similar level over 96 h. The medium containing xylose was utilized the best, and that containing sucrose was utilized slightly less than media containing the other carbon sources.

 Discussion Identification of <italic>Cytospora</italic> Species

Members of Cytospora have been reported as plant pathogens in forest and urban trees, including Anacardiaceae, Elaeagnaceae, Fabaceae, Juglandaceae, Myrtaceae, Rosaceae, Salicaceae, and Ulmaceae (Ehrenberg, 1818; Adams et al., 2002; Adams et al., 2005; Adams et al., 2006; Mehrabi et al., 2011; Wang et al., 2011; Fan et al., 2014a; Fan et al., 2014b; Fan et al., 2015a; Fan et al., 2015b; Lawrence et al., 2017; Fan et al., 2020). Previously, the identification of Cytospora relied on morphology and host affiliation. However, host affiliation is not always stable, and some species have similar morphology and uninformative illustrations and descriptions (Teng, 1963; Tai, 1979; Wei, 1979; Adams et al., 2002; Adams et al., 2005; Wang et al., 2011; Fan et al., 2014a; Fan et al., 2014b; Lawrence et al., 2017; Fan et al., 2020; Pan et al., 2020). ITS sequences were first used in Cytospora species identification by Adams et al. (2002), with six Cytospora groups suggested. Twenty-eight Cytospora species have been described from Eucalyptus, and 144 strains representing 20 species of Cytospora were subsequently collected from Iran (Adams et al., 2005; Fotouhifar et al., 2010). Recently, morphology combined with multi-locus phylogeny has been used for species identification, and has revealed many cryptic species (Fan et al., 2014a; Fan et al., 2015a; Fan et al., 2015b; Lawrence et al., 2017; Lawrence et al., 2018; Norphanphoun et al., 2018; Shang et al., 2020).

Fan et al. (2020) used six genes (ITS, LSU, act, rpb2, tef1-α, and tub2) to summarize 52 Cytospora species in China. Nevertheless, hidden fungal diversity has been revealed continuously in some special plant hosts (Pan et al., 2020; Pan et al., 2021). The present study revealed three species associated with Corylus heterophylla, i.e., Cytospora corylina, C. curvispora, and C. leucostoma. As the LSU gene is only available for a few species, we adapted a five-gene sequence of ITS, act, rpb2, tef1-α, and tub2 in the phylogeny analyses. Moreover, although C. leucostoma has been reported as a common species that causes canker in plants of Rosaceae, we have a poor understanding of its host specificity and pathogenicity (Pan et al., 2020). This study represents an attempt to enrich the study of Cytospora in China.

 Identification of <italic>Diaporthe</italic> Species

Diaporthe was established by Nitschke (1870) and has been extensively studied by Udayanga et al. (2011; 2012a) in recent years. Species of this genus, including endophytes, saprobes, and plant pathogens, are widely distributed in natural ecosystems (Udayanga et al., 2011; Udayanga et al., 2012a).

The species were initially determined based on host affiliations and morphological features (Aa et al., 1990). However, in terms of phylogenetic relationships, morphology and host association usually showed little siginificance (Brayford, 1990; Rehner and Uecker, 1994; Udayanga et al., 2014a; Udayanga et al., 2014b; Udayanga et al., 2015). Molecular techniques have been utilized in the latest taxonomic methods to define Diaporthe species (Santos and Phillips, 2009; Santos et al., 2010; Udayanga et al., 2011; Udayanga et al., 2012a; Udayanga et al., 2012b; Gomes et al., 2013; Udayanga et al., 2014a; Udayanga et al., 2014b; Udayanga et al., 2015). Since these revolutionary studies, more than 50 novel Diaporthe species have been identified in China (Huang et al., 2013; Huang et al., 2015; Gao et al., 2016; Gao et al., 2017; Yang et al., 2017a; Yang et al., 2017b; Yang et al., 2018; Fan et al., 2018; Yang et al., 2020). New records and species have been reported on the basis of molecular evidence (Rossman et al., 2015; Dissanayake et al., 2017; Guarnaccia and Crous, 2017; Perera et al., 2018; Tibpromma et al., 2018; Wanasinghe et al., 2018; Wrona et al., 2020).

In the current study, based on multi-locus sequences (ITS, cal, his3, tef1-α, and tub2), we identified two Diaporthe species associated with hazelnut. The known species, D. eres, has been widely reported as a plant pathogen. The other was identified as a new species, D. corylicola, with highly supported clades and holomorphic morphology.

 Fungal Diversity Associated With Hazelnut

A great number of fungal pathogens associated with many Corylus species, especially C. avellana, have been identified based on molecular data and morphological characteristics in recent studies (Pinkerton et al., 1992; Chen et al., 2007; Guerrero and Pérez, 2013a; Guerrero and Pérez, 2013b; Linaldeddu et al., 2016; Wiman et al., 2019; Yang et al., 2020; Zhu et al., 2020). A broad list of fungi has been reported on different parts of hazel trees. For example, Anisogramma, Anthostoma, Diaporthe, Diaporthella, Diplodia, Dothiorella, and Gnomoniopsis are common fungi that inhabit branches in hazel trees in America, Chile, Italy, and Turkey (Gottwald and Cameron, 1980; Guerrero and Pérez, 2013a; Linaldeddu et al., 2016). Alternaria, Aspergillus, Botryosphaeria, Colletotrichum, Diaporthe, Fusarium, Pestalotiopsis, and Phoma are often isolated from fruits, especially in Turkey and the Caucasus region (Sezer and Dolar, 2016; Battilani et al., 2018; Arciuolo et al., 2020; Arciuolo et al., 2021). Although little information is available, Cytospora corylicola generally occurs in hazelnut growing areas in Europe (Salerno, 1961). These studies indicate great similarities among fungi in hazelnut cultivating areas in different geographical locations and environmental conditions. A recent report introduced Elsinoë coryli (Sphaceloma coryli) as a re-emerging pathogen on hazel trees in southern Italy, and nine other species were collected from symptomatic branches in Sardinia (Italy), causing serious economic losses (Lamichhane et al., 2014; Linaldeddu et al., 2016; Minutolo et al., 2016; Fan et al., 2017). Thus, several studies have confirmed the high fungal diversity associated with European hazelnut.

In the current study, 51 strains were isolated from the stems and branches of Corylus heterophylla collected from Beijing, China. Among them, 14 were identified as Cytospora and the other 37 as Diaporthe. Among them, Cytospora corylina and C. leucostoma were only isolated from branches and other three species were observed both on stems and branches. These results indicate that the diversity of fungi associated with hazelnut canker and dieback disease is greater than previously recognized. All species in this study, except Diaporthe eres, were discovered for the first time on hazel trees. Previously, Cytospora coryli and Diaporthe coryli were reported as pathogens on branches of Corylus mandshurica, which is related to and generally grows naturally alongside C. heterophylla (Yang et al., 2020; Zhu et al., 2020). Thus, we speculate that C. heterophylla has great potential to be infected by Cytospora coryli and Diaporthe coryli. Although many Cytospora and Diaporthe species are endophytes and saprobes, it is still unclear whether opportunistic species would transform into pathogens on new hosts or in different environmental conditions, or adapt to climate change (Wang et al., 2020). Furthermore, Cytospora leucostoma and Diaporthe eres are plant pathogens that have been collected from a wide range of woody hosts, and Diaporthe species have been reported as the main cause of hazelnut defects in the Caucasus region (Battilani et al., 2018; Wiman et al., 2019). Thus, extensive investigations and pathogenicity tests on the five species need a further study.

 Optimum Environment for Culturing Isolates

Most fungi can grow at 10–35°C, with the most suitable range between 20 and 30°C. The most suitable pH for mycelial growth is 5.0–6.5 (Elfar et al., 2013). For instance, the optimal growth temperature of Diaporthe sp. is 22°C (Strausbaugh and Dugan, 2017), and that of Cytospora hadianensis is 19.8°C (Zhou et al., 2020). Helton and Konicek (1962) isolated six Cytospora species and indicated that the optimum pH is approximately 4.5, with a temperature of 20–35°C. Similar results were obtained in this study; all five species tested grew on PDA at 5–30°C and a pH of 3.0–11.0, with an optimum temperature of 20–30°C and pH value of 4.0–7.0. However, Cytospora corylina incubated at 15 and 20°C showed special growth characteristics on PDA. It formed very fine mycelial strands, which radiated outward from the central portion of the plate, and did not have the same physical appearance as the rest of the mycelium. The special growth characteristics of Cytospora species have been observed by Konicek and Helton (1962a), who provided a reference to include these fine strands in the measurement. As a result, the optimum conditions for Cytospora species were 20–30°C and pH 4.0–7.0, and those for Diaporthe species were 20–30°C and pH 5.0–7.0. If one unusual factor alone is removed, such as the aberrant marginal growth of C. corylina on PDA, optimum temperature for Cytospora is 25–30°C.

The six carbon sources tested in the current study were efficiently utilized, although the utilization of sucrose by Cytospora corylina, C. curvispora, and Diaporthe eres was less than that of the other sources. Zhou et al. (2020) reported that utilization of galactose by Cytospora hadianensis is low, and Zhao et al. (2019) reported that the utilization of xylose by Lasiodiplodia vaccinii and L. theobromae is the lowest. The best overall growth results of the six Cytospora species tested were obtained with maltose. However, none of the species in our study had the best overall growth on maltose media, but all had a high ability to use galactose and xylose, except Cytospora leucostoma for xylose.

Cultured isolates of different species differ subtly in mycelial growth and pathogenicity under a given condition, and one species may have different characteristics under different conditions (Konicek and Helton, 1962a; Konicek and Helton, 1962b; Wang et al., 2020). The different growth characteristics and high adaptability to the environmental conditions of these Cytospora and Diaporthe species seem to justify their widespread occurrence. Biological characterization of these strains, combined with their host distribution, may be used for species identification and distribution prediction. However, as there are currently few details regarding pathogen biology, it is necessary to evaluate the effects of environmental conditions, such as temperature, pH, and carbon sources, on mycelium growth and pathogenicity.

 Data Availability Statement

The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/ Supplementary Material .

 Author Contributions

All authors have made extensive contributions to the work presented in the article. XF and CT: contributed to conception of the experiment. HG and MP: completed the experiment. HG: conducted the data analyses. HG: wrote the original manuscript. XF: reviewed and edited the draft. All authors contributed to the article and approved the submitted version.

 Funding

This research was funded by the National Natural Science Foundation of China (31670647), and College Student Research and Career-Creation Program of Beijing (202010022256).

 Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

 Acknowledgments

We are grateful to Xiaohong Liang and Jing Han (the Experimental Teaching Centre, College of Forestry, Beijing Forestry University) for providing installed scientific equipment in the whole process. XF would like to acknowledge the supporting of strain preservation of Chungen Piao and Minwei Guo (China Forestry Culture Collection Center, Chinese Academy of Forestry, Beijing). HG thanks Xinwei Zhu (Beijing Forestry University) for the assistance during this study.

 Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021.664366/full#supplementary-material

References Aa H. A. V. D. Noordeloos M. E. Gruyter J. D. (1990). Species Concepts in Some Larger Genera of the Coelomycetes. Stud. Mycol. 32, 3–19. Adams G. C. Roux J. Wingfield M. J. (2006). Cytospora Species (Ascomycota, Diaporthales, Valsaceae): Introduced and Native Pathogens of Trees in South Africa. Australas. Plant Pathol. 35, 521–548. doi: 10.1071/AP06058 Adams G. C. Roux J. Wingfield M. J. Common R. (2005). Phylogenetic Relationships and Morphology of Cytospora Species and Related Teleomorphs (Ascomycota, Diaporthales, Valsaceae) from Eucalyptus . Stud. Mycol. 52, 1–144. Adams G. C. Surve-Iyer R. S. Iezzoni A. (2002). Ribosomal DNA Sequence Divergence and Group I Introns within Leucostoma Species, L. Cinctum, L. Persoonii and L. parapersoonii sp. nov., Ascomycetes that Cause Cytospora Canker of Fruit Trees. Mycologia 94, 947–967. doi: 10.1080/15572536.2003.11833153 Adams G. C. Taylor J. W. (1993). Phylogenetic Utility of the Internal Transcribed Spacer of Nuclear Ribosomal DNA in Leucostoma and Valsa . Inoculum 44, 1. Arciuolo R. Camardo L. M. Chiusa G. Castello G. Genova G. Spigolon N. . (2021). Ecology of Diaporthe eres, the Causal Agent of Hazelnut Defects. PloS One 16, e0247563. doi: 10.1371/journal.pone.0247563 Arciuolo R. Santos C. Soares C. Castello G. Spigolon N. Chiusa G. . (2020). Molecular Characterization of Diaporthe Species Associated with Hazelnut Defects. Front. Plant Sci. 11, 611655. doi: 10.3389/fpls.2020.611655 Battilani P. Chiusa G. Arciuolo R. Somenzi M. Fontana M. (2018). Diaporthe as the Main Cause of Hazelnut Defects in the Caucasus Region. Phytopathol. Mediterr. 57, 320–333. doi: 10.14601/Phytopathol-Mediterr-22872 Brayford D. (1990). Variation in Phomopsis Isolates from Ulmus Species in the British Isles and Italy. Mycol. Res. 94, 691–697. doi: 10.1016/S0953-7562(09)80670-9 Carbone I. Kohn L. (1999). A Method for Designing Primer Sets for Speciation Studies in Filamentous Ascomycetes. Mycologia 91, 553–556. doi: 10.2307/3761358 Chen H. Mehlenbacher S. A. Smith D. C. (2007). Hazelnut Accessions Provide New Sources of Resistance to Eastern Filbert Blight. Hortscience 42, 466–469. doi: 10.21273/HORTSCI.42.3.466 Crous P. W. Gams W. Stalpers J. A. Robert V. Stegehuis G. (2004). MycoBank: An Online Initiative to Launch Mycology into the 21st Century. Stud. Mycol. 50, 19–22. Dissanayake A. J. Phillips A. J. L. Hyde K. D. Yan J. Y. Li X. H. (2017). The Current Status of Species in Diaporthe . Mycosphere 8, 1106–1156. doi: 10.5943/mycosphere/8/5/5 Doyle J. J. Doyle J. L. (1990). Isolation of Plant DNA from Fresh Tissue. Focus 12, 13–15. Du Z. Fan X. L. Hyde K. D. Yang Q. Liang Y. M. Tian C. M. (2016). Phylogeny and Morphology Reveal Two New Species of Diaporthe from Betula spp. in China. Phytotaxa 269, 90–102. doi: 10.11646/phytotaxa.269.2 Ehrenberg C. G. (1818). Sylvae Mycologicae Berolinenses (Berlin, Germany: Formis Teophili Bruschcke). Elfar K. Torres R. Díaz G. A. Latorre B. A. (2013). Characterization of Diaporthe Australafricana and Diaporthe spp. Associated with Stem Canker of Blueberry in Chile. Plant Dis. 97, 1042–1050. doi: 10.1094/PDIS-11-12-1030-RE Fan X. L. Barreto R. W. Groenewald J. Z. Bezerra J. D. P. Pereira O. L. Cheewangkoon R. . (2017). Phylogeny and Taxonomy of the Scab and Spot Anthracnose Fungus Elsinoë (Myriangiales, Dothideomycetes). Stud. Mycol. 87, 1–41. doi: 10.1016/j.simyco.2017.02.001 Fan X. L. Bezerra J. D. P. Tian C. M. Crous P. W. (2018). Families and Genera of Diaporthalean Fungi Associated with Canker and Dieback of Tree Hosts. Persoonia 40, 119–134. doi: 10.3767/persoonia.2018.40.05 Fan X. L. Bezerra J. D. P. Tian C. M. Crous P. W. (2020). Cytospora (Diaporthales) in China. Persoonia 45, 1–45. doi: 10.3767/persoonia.2020.45.01 Fan X. L. Hyde K. D. Liu M. Liang Y. M. Tian C. M. (2015a). Cytospora Species Associated with Walnut Canker Disease in China, Description of a New Species C. Gigalocus . Fungal Biol. 119, 310–319. doi: 10.1016/j.funbio.2014.12.011 Fan X. L. Hyde K. D. Yang Q. Liang Y. M. Ma R. Tian C. M. (2015b). Cytospora Species Associated with Canker Disease of Three Anti-Desertification Plants in Northwestern China. Phytotaxa 197, 227–244. doi: 10.11646/phytotaxa.197.4.1 Fan X. L. Liang Y. M. Ma R. Tian C. M. (2014a). Morphological and Phylogenetic Studies of Cytospora (Valsaceae, Diaporthales) Isolates from Chinese Scholar Tree, with Description of A New Species. Mycoscience 55, 252–259. doi: 10.1016/j.myc.2013.10.001 Fan X. L. Tian C. M. Yang Q. Liang Y. M. You C. J. Zhang Y. B. (2014b). Cytospora from Salix in Northern China. Mycotaxon 129, 303–315. doi: 10.5248/129.303 Fang Z. D. (1979). Research Methods of Plant Diseases. (Beijing: Chinese Agriculture Press). Fotouhifar K. B. Hedjaroude G. A. Leuchtmann A. (2010). ITS rDNA Phylogeny of Iranian Strains of Cytospora and Associated Teleomorphs. Mycologia 102, 1369–1382. doi: 10.2307/27920441 Gao Y. H. Liu F. Cai L. (2016). Unravelling Diaporthe Species Associated with Camellia . Syst. Biodivers. 14, 102–117. doi: 10.1080/14772000.2015.1101027 Gao Y. H. Liu F. Duan W. Crous P. W. Cai L. (2017). Diaporthe Is Paraphyletic. IMA Fungus 8, 153–187. doi: 10.5598/imafungus.2017.08.01.11 Glass N. L. Donaldson G. C. (1995). Development of Primer Sets Designed for Use with the PCR to Amplify Conserved Genes from Filamentous Ascomycetes. Appl. Environ. Microbiol. 61, 1323–1330. doi: 10.1128/AEM.61.4.1323-1330.1995 Gomes R. R. Glienke C. Videira S. I. R. Lombard L. Groenewald J. Z. Crous P. W. (2013). Diaporthe: A Genus of Endophytic, Saprobic and Plant Pathogenic Fungi. Persoonia 31, 1–41. doi: 10.3767/003158513X666844 Gottwald T. R. Cameron H. R. (1980). Disease Increase and the Dynamics of Spread of Canker Caused by Anisogramma anomala in European Filbert in the Pacific Northwest. Phytopathology 70, 1087–1092. doi: 10.1094/Phyto-70-1087 Graniti A. (1957). Risultati Di Inoculazioni Artificiali Conceppidi Cytospora Corylicola Sacc., Isolati Da Noccioli Colpiti Da Maldello Stacco in Sicilia. L’Italia. Forestale. e. Montana. 12, 93–98. Guarnaccia V. Crous P. W. (2017). Emerging Citrus Diseases in Europe Caused by Species of Diaporthe . IMA Fungus 8, 317–334. doi: 10.5598/imafungus.2017.08.02.07 Guerrero J. A. Pérez S. M. (2013a). First Report of Shoot Blight and Canker Caused by Diplodia Coryli in Hazelnut Trees in Chile. Plant Dis. 97, 144–144. doi: 10.1094/PDIS-07-12-0667-PDN Guerrero J. A. Pérez S. M. (2013b). First Report of Diaporthe Australafricana Caused Stem Canker and Dieback in European Hazelnut (Corylus Avellana L.) in Chile. Plant Dis. 97, 1657–1657. doi: 10.1094/PDIS-03-13-0286-PDN Harrington T. C. Rizzo D. M. (1999). “Structure and Dynamics of Fungal Populations,” in Defining Species in the Fungi. Ed. Worrall J. J. (Dordrecht: Springer), 43–71. Helton A. W. Konicek D. E. (1962). An Optimum Environment for the Culturing of Cytospora Isolates from Stone Fruits. I. Temperature. Mycopathol. Mycol. Appl. 16, 19–26. doi: 10.1007/BF02136177 Hu Y. H. (2016a). Corylus Heterophylla Fisch. Afforestation by Sowing. Shaanxi. For. Sci. Technol. 1, 21–29. Hu Y. H. (2016b). Occurrence and Control of Main Harmful Organisms in Liaoning Province. J. Liaoning. Forestry. Sci. Technol. 2, 76–78. Huang F. Hou X. Dewdney M. M. Fu Y. Chen G. Hyde K. D. . (2013). Diaporthe Species Occurring on Citrus in China. Fungal Divers. 61, 237–250. doi: 10.1007/s13225-013-0245-6 Huang F. Udayanga D. Wang X. Hou X. Mei X. Fu Y. . (2015). Endophytic Diaporthe Associated with Citrus: A Phylogenetic Reassessment with Seven New Species from China. Fungal Biol. 119, 331–347. doi: 10.1016/j.funbio.2015.02.006 Jiang N. Yang Q. Fan X. L. Tian C. M. (2020). Identification of Six Cytospora Species on Chinese Chestnut in China. MycoKeys 62, 1–25. doi: 10.3897/mycokeys.62.47425 Katoh K. Standley D. M. (2013). MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. Mol. Biol. Evol. 30, 772–780. doi: 10.1093/molbev/mst010 Kirk P. M. Canoon P. F. Minter D. W. Stalpers J. A. (2008). Ainsworth & Bisby’s Dictionary of the Fungi. 10rd Edn (Wallingford: CABI). doi: 10.1079/9780851998268.0000 Konicek D. E. Helton A. W. (1962a). An Optimum Environment for the Culturing of Cytospora Isolates from Stone Fruits. II. Carbon Sources. Mycopathol. Mycol. Appl. 16, 27–34. doi: 10.1007/BF02136178 Konicek D. E. Helton A. W. (1962b). An Optimum Environment for the Culturing of Cytospora Isolates from Stone Fruits. IV. Hydrogen-Ion Concentration. Mycopathol. Mycol. Appl. 16, 243–248. doi: 10.1007/BF02145736 Lamichhane J. R. Fabi A. Varvaro L. (2014). Summer Heat and Low Soil Organic Matter Influence Severity of Hazelnut Cytospora Canker. Phytopathology 104, 387–395. doi: 10.1094/PHYTO-05-13-0136-R Lawrence D. P. Holland L. A. Nouri M. T. Travadon R. Abramians A. Michailides T. J. . (2018). Molecular Phylogeny of Cytospora Species Associated with Canker Diseases of Fruit and Nut Crops in California, with the Descriptions of Ten New Species and One New Combination. IMA Fungus 9, 333–370. doi: 10.5598/imafungus.2018.09.02.07 Lawrence D. P. Travadon R. Pouzoulet J. Rolshausen P. E. Wilcox W. F. Baumgartner K. (2017). Characterization of Cytospora Isolates from Wood Cankers of Declining Grapevine in North America, with the Descriptions of Two New Cytospora Species. Plant Pathol. 5, 713–725. doi: 10.1111/ppa.12621 Linaldeddu B. T. Deidda A. Scanu B. Franceschin A. Alves A. Abdollahzadeh J. . (2016). Phylogeny, Morphology and Pathogenicity of Botryosphaeriaceae, Diatrypaceae and Gnomoniaceae Associated with Branch Diseases of Hazelnut in Sardinia (Italy). Eur. J. Plant Pathol. 146, 259–279. doi: 10.1007/s10658-016-0912-z Liu Y. L. Whelen S. Hall B. D. (1999). Phylogenetic Relationships Among Ascomycetes: Evidence from an RNA Polymerse II Subunit. Mol. Biol. Evol. 16, 1799–1808. doi: 10.1093/oxfordjournals.molbev.a026092 Liu J. Zhang H. Cheng Y. Kafkas S. Güney M. (2014a). Pistillate Flower Development and Pollen-Tube Growth Mode During the Delayed Fertilization Stage in Corylus Heterophylla Fisch. Plant Reprod. 27, 145–152. doi: 10.1007/s00497-014-0248-9 Liu J. Zhang H. Cheng Y. Wang J. Zhao Y. Geng W. (2014b). Comparison of Ultrastructure, Pollen Tube Growth Pattern and Starch Content in Developing and Abortive Ovaries During the Progamic Phase in Hazel. Front. Plant Sci. 5, 528. doi: 10.3389/fpls.2014.00528 Mehrabi M. E. Mohammadi G. E. Fotouhifar K. B. (2011). Studies on Cytospora Canker Disease of Apple Trees in Semirom Region of Iran. J. Agric. Technol. 7, 967–982. Minutolo M. Nanni B. Scala F. Alioto D. (2016). Sphaceloma Coryli: A Reemerging Pathogen Causing Heavy Losses on Hazelnut in Southern Italy. Plant Dis. 100, 548–554. doi: 10.1094/PDIS-06-15-0664-RE Mostert L. Crous P. W. Kang J. C. Phillips A. J. (2001). Species of Phomopsis and A Libertella sp. Occurring on Grapevines with Specific Reference to South Africa: Morphological, Cultural, Molecular and Pathological Characterization. Mycologia 93, 146–167. doi: 10.2307/3761612 Nitschke T. (1870). Pyrenomycetes Germanici 2 (Breslau, Germany: Verlag Von Eduard Trewendt). Norphanphoun C. Doilom M. Daranagama D. A. Phookamsak R. Wen T. C. Bulgakov T. S. . (2017). Revisiting the Genus Cytospora and Allied Species. Mycosphere 8, 51–97. doi: 10.5943/mycosphere/8/1/7 Norphanphoun C. Raspé O. Jeewon R. Wen T. C. Hyde K. D. (2018). Morphological and Phylogenetic Characterisation of Novel Cytospora Species Associated with Mangroves. MycoKeys 38, 93–120. doi: 10.3897/mycokeys.38.28011 Özdemir M. Seyhan C. D. Bakan A. K. I̊lter S. Özay G. Devres O. (2001). Analysis of Internal Browning of Roasted Hazelnuts. Food Chem. 73, 191–196. doi: 10.1016/S0308-8146(00)00273-9 Pan M. Zhu H. Y. Bonthond G. Tian C. M. Fan X. L. (2020). High Diversity of Cytospora Associated with Canker and Dieback of Rosaceae in China, with 10 New Species Described. Front. Plant Sci. 11, 690. doi: 10.3389/fpls.2020.00690 Pan M. Zhu H. Y. Tian C. M. Huang M. R. Fan X. L. (2021). Assessment of Cytospora Isolates from Conifer Cankers in China, with the Descriptions of Four New Cytospora Species. Front. Plant Sci. 12, 636460. doi: 10.3389/fpls.2021.636460 Perera R. H. Hyde K. D. Dissanayake A. J. Jones E. B. G. Liu J. K. Wei D. . (2018). Diaporthe Collariana sp. nov., with Prominent Collarettes Associated with Magnolia Champaca Fruits in Thailand. Stud. Fungi 3, 141–151. doi: 10.5943/sif/3/1/16 Pinkerton J. N. Johnson K. B. Theiling K. M. Griesbach J. A. (1992). Distribution and Characterization of the Eastern Filbert Blight Epidemic in Western Oregon. Plant Dis. 76, 1179–1182. doi: 10.1007/BF00037702 Rayner R. W. (1970). A Mycological Colour Chart. (Kew: Commonwealth Mycological Institute). Rehner S. A. Uecker F. A. (1994). Nuclear Ribosomal Internal Transcribed Spacer Phylogeny and Host Diversity in the Coelomycete Phomopsis . Canndian. J. Botany-revue. Can. Bot. 72, 1666–1674. doi: 10.1139/b94-204 Ronquist F. Huelsenbeck J. P. (2003). MrBayes 3: Bayesian Phylogenetic Inference Under Mixed Models. Bioinformatics 19, 1572–1574. doi: 10.1093/bioinformatics/btg180 Rossman A. Y. Adams G. C. Cannon P. F. Castlebury L. A. Crous P. W. Gryzenhout M. . (2015). Recommendations of Generic Names in Diaporthales Competing for Protection or Use. IMA Fungus 6, 145–154. doi: 10.5598/imafungus.2015.06.01.09 Rossman A. Y. Farr D. F. Castlebury L. A. (2007). A Review of the Phylogeny and Biology of the Diaporthales. Mycoscience 48, 135–144. doi: 10.1007/S10267-007-0347-7 Salerno M. (1961). Cytospora Corylicola Sacc. E Patogenesi Del «Mal Dello Stacco» Del Nocciolo (Corylus avellana L.) in Sicilia. Rivista. Di Patol. Vegetale. 1, 38–64. Santos J. M. Correia V. G. Phillips A. J. L. (2010). Primers for Matingtypediagnosis in Diaporthe and Phomopsis: Their Use in Teleomorphinduction in Vitro and Biological Species Definition. Fungal Biol. 114, 255–270. doi: 10.1016/j.funbio.2010.01.007 Santos J. M. Phillips A. J. L. (2009). Resolving the Complex of Diaporthe (Phomopsis) Species Occurring on Foeniculum Vulgare in Portugal. Fungal Divers. 34, 111–125. doi: 10.1002/yea.1657 Servazzi O. (1950). Brevi Notizie Sulla «Moria» O «Seccume» Del Nocciolo Gentile Delle Langhe. Nuovo. Giornale. Bot. Italiano. 57, 679–682. Sezer A. Dolar F. S. (2016). “Hazelnut Kernel Defects and Associated Fungi in Three Provinces in Turkey. VII International Scientific Agriculture Symposium “Agrosym 2016”, in Book of Proceeding, VII. Ed. Kovačević D. , 1312–1318. Shang Q. J. Hyde K. D. Camporesi E. Maharachchikumbura S. S. N. Norphanphoun C. Brooks S. . (2020). Additions to the Genus Cytospora with Sexual Morph in Cytosporaceae. Mycosphere 11, 189–224. doi: 10.5943/mycosphere/11/1/2 Spielman L. J. (1985). A Monograph of Valsa on Hardwoods in North America. Can. J. Bot. 63, 1355–1378. doi: 10.1139/b85-190 Stamatakis A. (2006). Raxml-VI-HPC: Maximum Likelihood-Based Phylogenetic Analyses with Thousands of Taxa and Mixed Models. Bioinformatics 22, 2688–2690. doi: 10.1093/bioinformatics/btl446 Strausbaugh C. A. Dugan F. (2017). A Novel Penicillium sp. Causes Rot in Stored Sugar Beet Roots in Idaho. Plant Dis. 101, 1781–1787. doi: 10.1094/PDIS-03-17-0410-RE Sun J. (2013). Preliminary Report on Pathogen Identification of a New Disease of Corylus heterophylla in Liaoning Province. China Fruits. 86, 62–63. Swofford D. L. (2003). Paup*: Phylogenetic Analysis Using Parsimony (*and Other Methods) Version 4.0b10 (Sunderland, UK: Sinauer Associates). Tai F. L. (1979). Sylloge Fungorum Sinicorum (Beijing: Science Press). Tamura K. Stecher G. Peterson D. Filipski A. Kumar S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol. 30, 2725–2729. doi: 10.1093/molbev/mst197 Teng S. C. (1963). Fungi of China (Beijing: Science Press). Tibpromma S. Hyde K. D. Bhat J. D. Mortimer P. E. Xu J. C. Promputtha I. . (2018). Identification of Endophytic Fungi from Leaves of Pandanaceae Based on Their Morphotypes and DNA Sequence Data from Southern Thailand. Mycokeys 33, 25–67. doi: 10.3897/mycokeys.32.23670 Udayanga D. Castlebury L. A. Rossman A. Y. Chukeatirote E. Hyde K. D. (2014b). Insights into the Genus Diaporthe: Phylogenetic Species Delimitation in the D. Eres Species Complex. Fungal Divers. 67, 203–229. doi: 10.1007/s13225-014-0297-2 Udayanga D. Castlebury L. A. Rossman A. Y. Chukeatirote E. Hyde K. D. (2015). The Diaporthe Sojae Species Complex: Phylogenetic Re–Assessment of Pathogens Associated with Soybean, Cucurbits and Other Field Crops. Fungal Biol. 119, 383–407. doi: 10.1016/j.funbio.2014.10.009 Udayanga D. Castlebury L. A. Rossman A. Y. Hyde K. D. (2014a). Species Limits in Diaporthe: Molecular Re-Assessment of D. citri, D. cytosporella, D. foeniculina and D. rudis . Persoonia 32, 83–101. doi: 10.3767/003158514X679984 Udayanga D. Liu X. Crous P. W. McKenzie E. H. Chukeatirote E. Hyde K. D. (2012b). A Multi-Locus Phylogenetic Evaluation of Diaporthe (Phomopsis). Fungal Divers. 56, 157–171. doi: 10.1007/s13225-012-0190-9 Udayanga D. Liu X. McKenzie E. H. Chukeatirote E. Bahkali A. H. Hyde K. D. (2011). The Genus Phomopsis: Biology, Applications, Species Concepts and Names of Common Phytopathogens. Fungal Divers. 50, 189–225. doi: 10.1007/s13225-011-0126-9 Udayanga D. Liu X. Mckenzie E. H. Chukeatirote E. Hyde K. D. (2012a). Multi-Locus Phylogeny Reveals Three New Species of Diaporthe from Thailand. Cryptogam. Mycol. 33, 295–309. doi: 10.7872/crym.v33.iss3.2012.295 Wanasinghe D. N. Phukhamsakda C. Hyde K. D. Jeewon R. Lee H. B. Jones E. B. G. . (2018). Fungal Diversity Notes 709–839: Taxonomic and Phylogenetic Contributions to Fungal Taxa with an Emphasis on Fungi on Rosaceae. Fungal Divers. 89, 1–236. doi: 10.1007/s13225-018-0395-7 Wang X. Shi C. M. Gleason M. L. Huang L. (2020). Fungal Species Associated with Apple Valsa Canker in East Asia. Phytopathol. Res. 2, 35. doi: 10.1186/s42483-020-00076-5 Wang X. L. Wei J. Huang L. L. Kang Z. (2011). Re-Evaluation of Pathogens Causing Valsa Canker on Apple in China. Mycologia 103, 317–324. doi: 10.3852/09-165 Wei J. C. (1979). Identifcation of Fungus Handbook (Shanghai: Science Press). White T. J. Bruns T. Lee S. Taylor J. (1990). Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. PCR Protoc.: Guide. to Methods Appl. 18, 315–322. doi: 10.1016/B978-0-12-372180-8.50042-1 Wiman N. G. Webber J. B. Wiseman M. Merlet L. (2019). Identity and Pathogenicity of Some Fungi Associated with Hazelnut (Corylus Avellana L.) Trunk Cankers in Oregon. PloS One 14, e0223500. doi: 10.1371/journal.pone.0223500 Wrona C. J. Mohankumar V. Schoeman M. H. Tan Y. P. Shivas R. G. Jrff-Ego O. S. . (2020). Phomopsis Husk Rot of Macadamia in Australia and South Africa Caused by Novel Diaporthe Species. Plant Pathol. 69, 911–921. doi: 10.1111/ppa.13170 Yang Q. Fan X. L. Du Z. Tian C. M. (2017a). Diaporthe Species Occurring on Senna Bicapsularis in Southern China, with Descriptions of Two New Species. Phytotaxa 302, 145–155. doi: 10.11646/phytotaxa.302.2.4 Yang Q. Fan X. L. Du Z. Tian C. M. (2017b). Diaporthe Juglandicola sp. nov. (Diaporthales, Ascomycetes), Evidenced by Morphological Characters and Phylogenetic Analysis. Mycosphere 8, 817–826. doi: 10.5943/mycosphere/8/5/3 Yang Q. Fan X. L. Guarnaccia V. Tian C. M. (2018). High Diversity of Diaporthe Species Associated with Dieback Diseases in China, with Twelve New Species Described. MycoKeys 39, 97–149. doi: 10.3897/mycokeys.39.26914 Yang Q. Jiang N. Tian C. M. (2020). Three New Diaporthe Species from Shaanxi Province, China. MycoKeys 67, 1–18. doi: 10.3897/myookeys.67.49483 Zhang L. Alvarez L. V. Bonthond G. Tian C. M. Fan X. L. (2019). Cytospora Elaeagnicola sp. nov. Associated with Narrow-Leaved Oleaster Canker Disease in China. Mycobiology 47 (3), 1–10. doi: 10.1080/12298093.2019.1633902 Zhang Y. Li F. Tao R. Li Z. Liang Y. (2007). An Investigation of Wild Corylus Resource At Changbai Mountains. J. Jilin. Agric. Sci. 32, 56–57. Zhao L. Wang Y. He W. Zhang Y. (2019). Stem Blight of Blueberry Caused by Lasiodiplodia vaccinii sp. nov. in China. Plant Dis. 103, 2041–2050. doi: 10.1094/PDIS-01-19-0079-RE Zhou X. Pan M. Li H. Y. Tian C. M. Fan X. L. (2020). Dieback of Euonymus Alatus (Celastraceae) Caused by Cytospora haidianensis sp. nov. in China. Forests 11, 524. doi: 10.3390/f11050524 Zhu H. Y. Pan M. Bezerra J. D. P. Tian C. M. Fan X. L. (2020). Discovery of Cytospora Species Associated with Canker Disease of Tree Hosts from Mount Dongling of China. Mycokeys 62, 97–121. doi: 10.3897/mycokeys.62.47854 Zhu H. Y. Tian C. M. Fan X. L. (2018). Multigene Phylogeny and Morphology Reveal Cytospora spiraeae sp. nov. (Diaporthales, Ascomycota) in China. Phytotaxa 338, 49–62. doi: 10.11646/phytotaxa.338.1.4