AUTHOR=Huang Juntao , Lin Yanfa , Huang Zifeng , Li Tiantian , Ge Xiaodong , Liu Bin , Huang Zirui 

TITLE=Anti-diabetic effects of Dachaihu decoction combined with Sanghuangporus vaninii: insights from network pharmacology, intestinal flora, and liver metabolomics

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=Volume 13 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2025.1707392

DOI=10.3389/fcell.2025.1707392

ISSN=2296-634X

ABSTRACT=Numerous studies have demonstrated the therapeutic effects of the traditional Chinese medicinal fungus Sanghuangporus vaninii on type 2 diabetes mellitus (T2DM), yet the underlying mechanisms remain unclear. This study aimed to investigate the anti-diabetic and hypolipidemic effects of the S. vaninii combined with Dachaihu decoction formulation (DCHD-SV) in T2DM mice and deeply explore its action mechanisms through network pharmacology, 16S RNA sequencing, and liver metabolomics. The results showed that DCHD-SV can effectively ameliorate relevant symptoms of T2DM in mice, including hyperglycemia, dyslipidemia, insulin resistance, and organ damage. In addition, DCHD-SV modulates the intestinal flora and liver metabolites of T2DM mice, and may exert its anti-diabetic effects through the PPARγ/AMPK/mTOR signaling pathway. These findings underscore DCHD-SV as a promising candidate for T2DM therapy and lay a theoretical foundation for further investigating its mechanisms.