The main limitations in lipase-based biodiesel production are the high cost of the biocatalyst (Lv et al., 2021) along with the fact that native lipases are not optimized to function under harsh industrial conditions, e.g., high temperature, wide range of pH and presence of organic solvents (Ren et al., 2011; Zhong et al., 2021). To overcome these challenges, extensive studies are conducted on lipase immobilization, proving that immobilized lipases have great industrial potential as they overcome the free-lipases associated limitations and facilitate enzyme recovery (Venkatesagowda et al., 2018). Immobilization can increase the overall efficiency and selectivity of the enzymes by providing a controlled environment for the reaction. This results in improved substrate accessibility and facilitates better interaction between the enzyme and the reactants, leading to higher conversion rates. Upon interaction with interfaces of water and oil, lipases undergo interfacial activation, a phenomenon that involves the transition from their inactive (closed) form to their active (open) one, exposing thus hydrophobic areas of the protein to the surface. Thus, in the presence of hydrophobic materials like some immobilization carriers, lipases are usually hyperactivated when immobilized on hydrophobic carriers (Bastida et al., 1998). Strategies such as careful selection of immobilization methods, engineering of active sites, and the use of hybrid enzyme systems (Wang et al., 2023; Xu et al., 2020; dos Santos et al., 2014) can contribute to improved specificity and overall performance in the biodiesel synthesis process (Tan et al., 2023). Immobilized enzymes can be used for more than one reaction cycle with little loss in activity. Moreover, the immobilized catalyst can be easily separated from the reaction media (Marín-Suárez, et al., 2019). Immobilization processes are generally based on covalent bonding, physical adsorption, entrapment, and encapsulation (Filho et al., 2019), using an extended set of supports, varying from naturally occurring carriers (olive kernel, rice husk, diatomite, etc.) (Yücel, 2011; Meunier and Legge, 2012; Cespugli et al., 2018) to synthetic carriers (nanomaterials, alginate, activated carbon, etc.) (Pinto Brito et al., 2020). Herein, we review selected representative works on the utilization of immobilized lipases to produce biodiesel from low quality oils.

Wang et al. (2017) used MAS1 lipase from marine Streptomyces sp. strain W007 immobilized onto XAD1180 resin to convert waste cooking oil, achieving 95.45% yield in 24 h. The optimal reaction conditions were 30°C, methanol to oil ratio (MOR) 3:1 (with one-step addition of methanol), enzyme loading of 80 U/g substrate, and 200 rpm agitation. This biocatalyst lost 30% of its initial activity after four cycles. When compared with commercial immobilized enzymes, namely, Novozym 435, Lipozyme RM IM and Lipozyme TL IM, it performed significantly better, when all methanol was added in one step in the start of the process.

Teixeira et al. (2017) used two acidic oils of macauba (Acrocomia aculeata) as substrates to produce biodiesel with Lipozyme 435. The first acidic oil had 42.6% acidity and 5,000 ppm water content (WC), while the second oil had 36.9% acidity and 3,484 ppm WC. They reported that 5% w/w enzyme, MOR 2:1 and incubation time at 30°C lead to 91.7% yield in 4 h (Teixeira et al., 2017).

Lipase from Burkholderia cepacia immobilized onto mesoporous silica/iron oxide magnetic coreshell nanoparticles, using canola waste cooking oil, provided 85.2% yield in 25 h (Khoobbakht, et al., 2020). In this case, the optimal reaction conditions were 34°C, 36% biocatalyst, and MOR 3:1. The performance of the immobilized B. cepacia lipase was decreasing already from the third cycle, losing 11% of its initial activity. In another work, lipase from porcine pancreas immobilized on genipin cross-linked chitosan successfully converted waste cooking oil to biodiesel with 92.33% yield in 10 h, performing equally efficiently for six cycles (Khan et al., 2020). The optimal reaction conditions were, 40°C, 6% v/v WC, catalyst loading 7.5% wt immobilized enzyme, MOR 9:1, and 150 rpm agitation. Aspergillus niger lipase immobilized on mesoporous silica material SBA-15 was very recently investigated as biocatalyst for biodiesel production, using Calophyllum inophyllum oil as a non-edible renewable resource (Arumugam and Ponnusami, 2023). Its application at 30°C, MOR 6:1, 15% v/v WC (pH 7.0) and 500 rpm agitation led to 97% yield in 8 h.

In case of combi-enzymatic systems, Poppe et al. (2018a) suggested one with lipase B from Candida antarctica (CALB), lipase from Thermomyces lanuginosus (TLL), and RML. The substrates used for the transesterification reactions were waste oil and soybean oil. Using soybean oil as substrate, molar ratio of ethanol:soybean oil was 8.09:1 and combi-lipase composition of 22.5% TLL, 50% CALB, and 27.5% RML. Using waste oil as substrate, molar ratio of ethanol:waste oil was 9:1, and the combi-lipase composition was 40% TLL, 35% CALB, and 25% RML. The reactions were performed at 40°C under agitation for both substrates. Yield, was about 50%, with average productivity of 1.94 g_{ethyl esters}/g_substrate/h, for both oils (Poppe et al., 2018a). The group evaluated the same system but after ultrasound-assisting the reactions (Poppe et al., 2018b). The reactions were carried out at 40°C for 18 h, with a 6:1 ethanol:oil molar ratio and 15% biocatalyst (by oil mass). The highest yield achieved was 90%, using soybean oil, and 70% when using the waste oil. Concerning the effect of the lipases in the reactions with waste oil, the greatest yield was obtained using pure CALB as biocatalyst, whereas for soybean oil, the highest yield was obtained with a combination 50% CALB and 50% RML.

Binhayeeding et al. (2020a), produced biodiesel (96.5% yield) from waste cooking oil in 24 h, by using Candida rugosa and Rhizomucor miehei lipase (CRL and RML, respectively) immobilized on poly (3-hydroxybutyrate) beads. The optimal reaction conditions were 45°C, 5% w/w WC, 1% of combi-lipase (ratio 1:1), MOR 6:1 and 250 rpm agitation. This combi-lipase system could be reused in six reaction cycles without losing activity (Binhayeeding et al., 2020b). The same combi-lipase system was used for biodiesel synthesis from by-products of chicken industry. Fats and skin of chicken could be converted into biodiesel (97.1% yield) in 12 h, by a mixture of CRL (1.5% wt) and RML (1% wt), immobilized on polyhydroxybutyrate (PHB) beads (Binhayeeding et al., 2020a). Here, the best conversion conditions were 40°C, 2.5% wt total enzyme loading, 5% w/w WC, MOR 6:1, and agitation 200 rpm. The combi-lipases could retain 50% of its initial activity for seven reaction cycles.

As stated above, lipases have great potential in industrial and biotechnological processes, like detergents (de Oliveira et al., 2020), flavour enchanters (Kendirci et al., 2020), oleochemicals (Abdelmoez et al., 2013), and pharmaceuticals (Contesini et al., 2020) manufacturing. However, the major drawback of the lipase-based processes is the high cost of the biocatalyst. Development of recovery and reuse technologies, mainly through their physical or chemical immobilization in suitable carriers, is mandatory to alleviate this downside. Apart from recovery and reuse, immobilization also facilitates the stabilization of enzymes under conditions that would not be tolerated by their free counterpart (Bilal et al., 2021). Innumerable materials categorized in organic, inorganic, hybrid, physical or artificial polymers and nanostructured matrices are under evaluation as potent immobilization materials (Pavlidis et al., 2014; Ismael and Baek, 2020). Various renewable materials are employed as immobilization matrices for enzymes, providing a supportive environment for their catalytic activity. Some common types include cellulose-based material, agarose (Arana-Peña et al., 2020), alginate, chitosan (de Oliveira et al., 2017), silica-based materials, polymeric materials, nanoparticles (de Oliveira et al., 2020) and magnetic nanoparticles (López et al., 2014; Gireli and Chiappini, 2023). Considerations such as biocompatibility, pore size, chemical stability, ease of preparation, mechanical strength, and recyclability (Mohidem et al., 2023), all contribute to the successful application of immobilized enzymes in various industrial processes (Mokhtar et al., 2020). This paragraph outlines indicative novel, eco-friendly, and low-cost materials as carrier matrices for lipase immobilization.

One of the major agricultural wastes is the rice husk produced during the milling of rice (Oryza sativa). According to Food and Agricultural Organization, the forecasted global production of rice in 2022/2023 would be 519.5 million metric tons, and for every 1 kg rice produced, approximately 0.28 kg rice husk is generated. Several groups have investigated its application as a matrix for enzyme immobilization. Costa-Silva et al. (2016) tested the production of biodiesel using a lipase from a fungal plant pathogen, named Cercospora kikuchii. The lipase was immobilized by covalent binding on rice husk, resulting in a loading of 5.5 mg_protein/g_support (75%–80% immobilization efficiency). The residual lipase activity of the immobilizate was 75%, while in its free form was 12%, indicating a significant stabilization effect of the immobilization process to the enzyme. As for the reusability of the immobilized lipase, after five cycles of use the immobilizate maintained 87.5% of its initial activity. This preparation was used for the production of biodiesel from coconut oil, transesterified with anhydrous ethanol in a molar ratio of 1:12, using tert-butanol as a solvent. The reaction was performed at 50°C for 120 h under 150 rpm agitation. Results showed 97.1% FAEE yield after 72 h, meeting the requirements of ASTM D6751, and the potential to be used as alternative biofuel (Costa-Silva et al., 2016). In another work, Bonet-Ragel and coworkers (2018) investigated the application of rice husk ash in the immobilization of a recombinant Rhizopus oryzae lipase (rROL). The researchers compared the physical adsorption of the lipase on rice husk ash (a waste product of the use of rice husk for power generation via gasification) to a commercial hydrophobic support (RelOD). However, in this case, the lipolytic activity was found 50% lower in rice husk ash than in RelOD (Bonet-Ragel et al., 2018), showcasing that the effect of immobilization matrix on the enzyme should be studied case by case. Cespugli et al. (2018) also suggested high immobilization yields of lipase B from C. antarctica and two other commercial asparaginases. Rice husk was first oxidized and functionalized with di-amino spacer for covalent immobilization. After 48 h, the immobilization of CALB on the oxidized rice husk reached 72% compared to 95% immobilization on a commercial methacrylic resin after 24 h. The hydrolytic activity of CALB on the functionalized rice husk was 316 U/g. The immobilized enzyme was used in a solvent free poly-condensation reaction.

Another significant agricultural bio-waste is the olive pomace, a side-stream of the olive oil industry. The TLL was covalently immobilized onto olive pomace, leading to a maximum loading of TLL of 18.67 mg_protein/g_support (Yücel, 2011). Using p-nitrophenyl palmitate (pNPP) as substrate, the specific hydrolytic activity of the immobilized enzyme reached 10.31 U/mg_protein and was retained for at least ten batch reactions. When olive pomace oil was used as a substrate for biodiesel production, and with a molar ratio of MOR 6:1, biodiesel yield was 93% at 25°C, 125 rpm agitation, in a 24 h reaction, almost achieving the required product purity (Yücel, 2011).

De Castro et al. (2022) suggested the immobilization through physical adsorption of the Botryosphaeria ribis EC-01 lipase on a low-cost and eco-friendly film, composed by cassava starch, polyvinyl alcohol, and sericin blend (CS–PVA–SS). Similarly, using a pNPP hydrolytic assay, they determined a 98.7% activity retention of the immobilized lipase. Their biocatalyst led to 95% esterification of oleic acid to ethyl oleate at 49°C in n-heptane, molar ratio ethanol:oleic acid of 3:1, 1.25 g lipase film after 30 h with 150 rpm agitation. However, the immobilization only slightly protected the protein, as after seven cycles of use the yield halved (de Castro et al., 2022).

Linsha et al. (2016), using pollen grains of Hibiscus rosa-sinensis to construct hierarchically porous alumino-siloxane aerogel, investigated the biocatalytic conversion of oleic acid to methyl oleate by immobilized steapsin lipase. The rare structural architecture was developed through a bio-templating method. The highest immobilization efficiency (∼95%) was obtained after the carrier was functionalized with amino propyl groups. This is the case with most biological materials, as specific functional groups are required for covalent immobilization, and their abundance is directly correlated with the maximum load. Concerning the potential application in biodiesel synthesis, methyl oleate yield reached 59% in a reaction that was held at 40°C, in n-hexane, with a molar ratio of alcohol:oleic acid was 6:1 (Linsha et al., 2016).

Tan et al. (2006) introduced the production of biodiesel using an immobilized Candida sp. lipase on a cheap cotton membrane at high WC. The biocatalyst performed at least six cycles at a WC of 15%–20%, yielding high conversion rates, up to 90%. The half-life of the immobilized lipase was more than 200 h. This immobilizate could be efficiently used for biodiesel production as after 30 h at 40°C, WC 15% (w/w), a MOR of 3:1, the conversion was up to 98% (Tan et al., 2006).

Khosla et al. (2017) suggested the immobilization of Pseudomonas sp. ISTPL3 lipase on biochar. Biochar is carbonized biomass produced from the pyrolysis of waste biomass, which due to the presence of surface functional groups, porosity, and moderate surface area, is an appealing material for enzyme immobilization (Pandey et al., 2020). The ISTPL3 lipase was immobilized through physical adsorption and covalent binding after biochar activation with phosphoric acid. Immobilization yield and efficiency were 83.04% and 62.86%, respectively, with 21.4% leaching in the first case (non-covalent binding) (Khosla et al., 2017). Reactions were performed with the lipids from Serratia ISTD04 with MOR 6:1, for 3 h at 300 rpm. The immobilized lipase gave the highest yield of FAMEs (92.23%) followed by non-immobilized lipase (87.81%), while the alkaline transesterification with NaOH yielded the lower amount of FAMEs (81.12%). The immobilized enzyme could be used for three subsequent biodiesel reaction cycles, retaining 75% of its initial activity (Khosla et al., 2017).

Besides lipases, cutinases appear very promising for biodiesel production (Serralha et al., 1998). Cutinases (EC 3.1.1.74) belong to the class of serine esterases, and to the superfamily of α/β hydrolases. They catalyze the hydrolysis, esterification, and transesterification of short and long chain esters, thus being very useful in the industry of detergents and biodiesel (Castro-Ochoa et al., 2012). The substrate of cutinases is a structural polymer lipid called cutin, which covers the plant epidermis and has a protective role. Cutin has a waxy texture and is made of long-chain fatty acids like palmitic acid (16:0) and oleic acid (18:1) joined by ester bonding, forming a steady three-dimensional structure (Bermúdez-García et al., 2017). Cutinases compete with lipases also for the enantioselective synthesis of chemicals and pharmaceuticals, as stated by Su et al. (2020). Lipases have been proven efficient for this task, given the resemblance of their active sites to tunnels, facilitating distinct orientation and binding of enantiomers. Unlike most lipases, cutinases have catalytic triads exposed to solvents, providing potential benefits in reactions by improving substrate accessibility and promoting product diffusion from active site. This characteristic might also enable catalytic reactions with sterically hindered substrates that struggle to reach confined active site clefts; thus, offering great potential in biodiesel industry.

Badenes et al. (2010) worked with the cutinase from the phytopathogenic fungus Fusarium solani pisi using TAGs as substrate. They reported as optimal conditions the 30°C, 600 rpm, 24 h reaction time and MOR of 3:1. In their case, methanolysis gave 75% conversion after 24 h. They could also show that at the same condition, the conversion could be increased, achieving 90% and 80% conversion, using 1-butanol or ethanol as alkyl donor, respectively. Bermúdez-García et al. (2017) observed similar behavior of a thermo-alkaline cutinase from Aspergillus nidulans when used to synthesize FAMEs from sesame oil. After optimizing the conditions (MOR 6:1, 2% w/v of lyophilized cutinase, 7% v/v WC, incubation at 60°C for 72 h at 250 rpm), and even adding methanol stepwise (every 24 h), they could only detect 2% conversion (Bermúdez-García et al., 2017).

The research team of Badenes and coworkers performed another work with a variant of F. solani pisi cutinase (mutant T179C), using a membrane reactor to produce biodiesel in organic media (Badenes et al., 2011a). They managed to produce up to 500 g_product/day/g_enzyme from TAGs. After a 24 h reaction, with a 1.6 ratio of alcohol to fatty acid chains and enzyme concentration varying from 0.5 mg/L to 1 mg/mL they determined 90% conversion of TAGs to alkyl esters, using methanol, ethanol, and butanol (Badenes et al., 2011a). The same enzyme was also used in a reversed micellar system for alkyl esters production using acid oil (Badenes et al., 2011b). The optimum reaction conditions were 30°C, 600 rpm stirring and molar ratio of alcohol to fatty acids of 1.6. After 24 h reaction time, the methyl ester conversion achieved was 64%–78%. They observed a 45% loss of cutinase activity when incubated in the micellar system for 3 h. In addition, there was a 90% activity loss in the presence of methanol within 10 min of incubation. In contrast, an improvement of cutinase performance was achieved when incubated with ethanol or butanol, indicating that these alcohols act protectively. Mutant T179C displayed high stability in the presence of methanol, with an activity loss of only 16% (Badenes et al., 2011b).

Acyltransferases also emerged as promising candidates for biodiesel production. For instance, Rodrigues et al. (2016) investigated the biocatalytic production of biodiesel using a lipase/acyltransferase from Candida parapsilosis (CpLIP2). This biocatalyst preferably catalyses alcoholysis over hydrolysis when in aqueous or in biphasic (aqueous/organic) media. In this work, CpLIP2 was immobilized with physical adsorption and subsequent cross-linked with glutaraldehyde (GA) on two synthetic resins, polypropylene (Accurel MP 1000) and divinyl-benzene crosslinked methacrylate polymer (Lewatit VP OC 1600). The immobilization yield of CpLIP2 on Lewatit VP OC 1600 and Accurel MP 1000 was 77% and 80%, respectively. The substrate used for biodiesel production was jatropha oil in a lipid/aqueous system. The oil acidity was 3.7%. Transesterification reactions were held at 30°C, under agitation, using 10% w/w of immobilized enzyme in relation to oil. The molar ratio of methanol:TAG was 6:1. After 8 h reaction time FAME yield reached 80.5%, for CpLIP2 immobilized on Accurel MP 1000% and 93.8% for the enzyme immobilized on Lewatit VP OC 1600 (Rodrigues et al., 2016).

In another work, Mandal et al. (2020) induced the expression of acyltransferases in Graesiella emersonii NC-M1 and Chlorophyta sp. NC-M5 by the addition of phytohormones under nitrogen-limited (NL) conditions. More specifically, they used indole acetic acid and kinetin to enhance biomass and lipid production; nevertheless, this had also an effect on the expression of GPAT and DGAT (glycerol-3-phosphate acyltransferase and diacylglycerol acyltransferase) under NL conditions. The whole cells were used as biocatalysts for biodiesel production. The FAMEs profile of G. emersonii NC-M1 grown under NL and in the presence of phytohormones showed a 44.8% decline in saturated fatty acid (SFAs) content, whereas 179% increase in monounsaturated fatty acid (MUFAs), in comparison to the standard conditions. The FAMEs profile of Chlorophyta sp. NC-M5 showed 96.5% polyunsaturated fatty acid (PUFAs) contents respectively, while 52.2% drop in SFAs and MUFAs under the same conditions. Mandal and co-workers suggested that these acyltransferases could be potentially used for biodiesel production; however, until now, no follow-up work has been published.

Immobilized lipases and whole-cell lipases are the most studied biocatalysts for biodiesel production. However, when whole cells are employed, the indirect enzyme immobilization within or on the cells, bypasses the isolation, purification, and immobilization steps; thus, simplifying the time-consuming and material-intensive upstream processing. Moreover, whole-cell biocatalysis allows significantly better product recovery rates, simplifying also downstream processing, and further decreasing environmental and economic costs (de Carvalho, 2017; Lin and Tao, 2017). Two types of whole-cell catalysts are used for biodiesel production, i.e., whole cells producing cell-bound or intracellular lipases encoded by either native or heterologous genes (Hwang et al., 2014; Borrelli and Trono, 2015; Chandra et al., 2020) and recombinant cells displaying lipases on their surface (Smith et al., 2015; Tanaka and Kondo, 2015; Han et al., 2018; Kuroda and Ueda, 2022b; Lotti et al., 2018; Ye et al., 2021; Kuroda and Ueda, 2022a; Teymennet-Ramirez et al., 2022).

The most commonly used native lipase producers belong to the fungal species Mucor, Rhizopus (e.g., R. oryzae, R. chinensis, R. miehei), Geotrichum, Rhizomucor, Aspergillus (e.g., A. oryzae), Penicillium, Thermomyces, Fusarium. However, R. oryzae lipase (ROL) is in the spotlight, due to its remarkable 1,3-regiospecificity that yields 2-monoacylglycerol instead of glycerol, a compound that lubricates and upgrades biodiesel characteristics (López-Fernández et al., 2020). Both lipase producing R. oryzae cells and purified ROL are employed for biodiesel production usually after immobilization using various materials with biomass support particles (BSPs) being the most commonly used (Table 1). Other native lipase producers belong to the bacterial Pseudomonas, Bacillus sp., and yeast Yarrowia lipolytica, Candida antarctica and Rhodotorula sp. (Table 1). Heterologous lipase encoding genes most commonly are overexpressed in hosts such as Pichia pastoris, Saccharomyces cerevisiae, Aspergillus oryzae, and Escherichia coli (Table 2). In the case of cell-bound or intracellular lipases, low mass transfer rate of high molecular weight substrates from the solvent to the biocatalyst, cellular metabolism, protein synthesis, and also the toxicity of the alcohol used, can be the rate-limiting steps for the whole-cell bioconversion of the substrate (Schrewe et al., 2013; Aarthy et al., 2014; Wachtmeister and Rother, 2016). Permeabilization of the cells using chemical (detergents and solvents) or physical (e.g., air-drying, temperature shock) means is often applied to improve limited substrate transfer across cell walls and membranes (Ni and Chen, 2004; Aguieiras et al., 2015; Lin and Tao, 2017; Sakkow et al., 2019). While permeabilization via chemical or physical treatment of cell membrane is effective in small-scale process, large-scale implementation is problematic. Molecular engineering approaches to enhance mass transfer recently emerged as a much better alternative (Chen, 2007). The co-expression of membrane transporters can be such strategy, as demonstrated for the biocatalytic conversion of C7-C16 n-alkanes by Grant et al. (2014), who improved the specific yields of bioxidation of > C12 alkanes to fatty alcohols and acids by up to 100-fold, when co-expressed in E. coli the alkL gene from Pseudomonas putida, encoding an alkane import protein. Immobilizing the lipase on the cell surface by yeast cell surface display (YCSD) is another molecular engineering strategy that can be employed to overcome mass transfer related limitations (Smith et al., 2015; Tanaka and Kondo, 2015; Han et al., 2018; Kuroda and Ueda, 2022b; Ye et al., 2021; Kuroda and Ueda, 2022a; Teymennet-Ramirez et al., 2022). CSD has been one of the most valuable tools to not only study and comprehend protein functions, but also to bestow yeast cells with novel properties, such as novel catalytic functions, affinity binding to ligands, bioremediation or bio-monitoring properties, library screening purposes, whole-proteome studies, vaccine and antibiotics development, biosensors production, etc. (Shibasaki et al., 2009). With CSD, target peptides or proteins are displayed on the cell surface after fusion with an anchoring protein system, which in yeast typically comprises a cell wall protein (CWP) linked to glycosylphosphatidylinositol (GPI) (Tanaka and Kondo, 2015; Chen, 2017). Apart from the target enzyme and the anchor protein, the linker sequence and the host microbial cell are also structural units playing crucial role in CSD. However, the interplay between these units and lipase conformation and activity are not yet fully understood.

“Arming” the yeast cell surface with enzymes, is nevertheless one of the most attractive applications of molecular display technology, given that yeast cells obtain novel potentials as whole-cell biocatalysts and innovative bioprocesses can be conceptualized (Shibasaki et al., 2009; Tabañag et al., 2018; Fan et al., 2020). The maturation of CSD technology occurred at the turn of the second (i.e., structural based biocatalyst engineering by rational design) and third (i.e., biocatalyst engineering by directed evolution) waves of biocatalysis evolution and enabled researchers to exploit its natural ability to link genotype and phenotype as powerful library screening tool for protein engineering (Bornscheuer et al., 2012; Smith et al., 2015). The construction of whole-cell biocatalysts using the YCSD technology has the benefits of combined fine-tuning of gene expression and “natural” enzyme immobilization; thus, generating renewable self-immobilized biocatalysts (Teymennet-Ramírez, 2022). Apart from mass transfer limitations, YCSD can overcome other serious drawbacks of the conventional immobilization techniques, such as structural and functional alterations, low enzyme loading, dissociation, costs of immobilization materials and process, etc. (Ueda and Tanaka, 2000). In addition, CSD enzyme allows whole cells to readily access soluble substrates while retaining the metabolic potential of their intracellular enzyme systems (e.g., extracellular degradation of cellulose and internalization of the major product, glucose, to produce ethanol) expanding the industrial applications of engineered biocatalysts in (Smith et al., 2015). CSD of Pichia pastoris, Yarrowia lipolytica, and Saccharomyces cerevisiae is widely used to construct whole-cell biocatalysts by expressing various heterologous lipase-encoding genes (Table 3). These systems display many advantages associated with safety, simplicity of genetic manipulation and rigidity of the cell-wall structure, standardized bioproduction of target proteins, use of the biocatalyst without purification and immobilization. Compared to S. cerevisiae, P. pastoris can achieve a much higher cell density in fermentation (Dong et al., 2020) and additionally, an indirect CSD method was developed that simply displays various enzymes with an average efficiency ten times higher than that of commonly used S. cerevisiae CSD methods (Li et al., 2019a). However, the exploitation of CSD lipases on yeast cells as whole-cell biocatalysts for biodiesel production is still very limited due to poor operational stability (Fu and Vasudevan, 2010; Liu et al., 2018). One of the main hurdles to increase the use of lipases in biodiesel industry is their low stability and tolerance under high methanol concentrations that are required. Since the stoichiometric ratio for the transesterification reaction requires 3 mol methanol and 1 mol TAGs to yield 3 mol FAMEs and 1 mol glycerol, the optimal methanol to oil ratios to promote the transesterification reaction should be higher than 3:1. A higher molar ratio though, can inactivate the enzyme, especially when the alcohol is insoluble in the reaction mixture (Huang et al., 2012; Lotti et al., 2018). Stepwise addition of methanol during the transesterification to keep its concentration at a relatively low level is generally applied to circumvent this limitation. Nonetheless, apart from the methanol to oil ratio, various other parameters have to be fine-tuned to make the transesterification reaction competitive. These parameters can be related to the overall process design (e.g., solvent quantity, solvent to oil ratio, temperature, biocatalyst concentration, WC to ensure active enzyme conformation), upstream strategies (e.g., type of raw materials used, pre-treatment, plant genetic engineering) and the biocatalyst performance (e.g., robustness, natural alcohol tolerance, protein engineering) (Hasheminejad et al., 2011; Lotti et al., 2018).

Even though many obstacles remain to overcome, the elimination of separation and purification steps that are bypassed and the utilization of cheap waste materials for cell cultivation (thus, further reducing process costs), make whole-cell lipases very attractive catalysts for biodiesel production. In addition, both protein and metabolic engineering can facilitate the creation of tailor-made biocatalysts with desirable properties. Novel engineered lipases or even combination of lipases (compi-lipases) displaying different or complementary activities and enhanced methanol tolerance can be employed to optimize production yields, decrease unwanted side-products (unconverted intermediates such as monoglycerols, diacylglycerols, free fatty acids), and also improve biodiesel quality (Ogino and Amoah, 2019). The bioprospecting of novel native lipase producing microbial strains can also contribute to expand the spectrum of potential lipolytic enzymes and inspire novel synthetic processes (Escobar-Niño et al., 2014; Sahoo et al., 2017; Hama et al., 2018; Chow et al., 2021). On the other hand, metabolic engineering tools can be employed to design and develop biodiesel-producing microbial cell factories capable, not only of in vitro biotransformation of various common oil feedstocks to biodiesel, but also of de novo biosynthesis of biodiesel from glucose, glycerol or even cellulosic biomass (Yan et al., 2017).

Enzymatic transesterification processes face similar challenges to conventional transesterification processes, namely, mass transfer limitation due to the biphasic reaction mixture, thermodynamic limitations due to the reaction reversibility in the absence of product removal, scale-up and mixing issues, and technical constrains when operating at continuous mode (Athar and Zaidi, 2020). Membrane technology is a process that uses semi-permeable membranes to separate different substances in a mixture. Membrane performance is usually driven by selectivity and permeability parameters, while membrane acts as a physical barrier in liquid-liquid and solid-liquid systems (Hajilary et al., 2019). Membrane integration in enzymatic transesterification (Figure 2) may be a promising alternative to conventional processes, since they may overcome the aforementioned challenges given that they offer an unconventional immobilization option; they achieve simultaneous transesterification and biodiesel separation; and they facilitate downstream processing of enzymatically produced biodiesel and biocatalyst’s reuse.

The biocatalytic approaches in biodiesel synthesis have been proven, in many cases, advantageous to the alkaline or acidic synthesis, as the reaction can be performed in low quality, non-edible oils, with high acidity levels, resulting in reduced process cost (Sales et al., 2022). Biocatalytic esterification/transesterification can even take place in the presence of water if, for instance, acyltransferases are employed. However, the methanol-induced enzymatic inhibition is one of the most challenging bottlenecks of the field so far. To overcome that, different bioreactor setups and processing modes, or even longer alcohols, such as ethanol or 1-butanol, have been evaluated. Protein engineering of lipases or other enzymes of interest, like cutinases, which seem to be affected the most, could provide variants that are more stable and do not suffer from methanol inhibition. The combination of these variants with whole-cell systems is expected to provide sustainable processes for the production of second-generation biodiesel.

Concerning the bioreactor setup, membrane reactors membranes may play several roles during biodiesel production and purification. First, membranes may act as a selective barrier for biodiesel impurities, such as soap, catalysts, and other contaminants. Also, the removal of glycerol, which is a by-product of transesterification process, either operating in batch or in continuous mode, leads in higher reaction and conversion rates of the biodiesel produced. UF membranes showed the best results for refining biodiesel, using mostly polymeric materials such as PES, PAN and regenerated cellulose. Another key role that the membranes can achieve is the concentration of liquid lipase mixtures after transesterification for their subsequent reuse, making lipase catalyzed production of biodiesel more economical. It should be noted also that bioreactors are often integrated with automation systems, allowing for the control and optimization of important parameters such as temperature, agitation, and pH. Thus, the application of such systems in biodiesel production is expected to increase significantly in the coming years.

Furthermore, in the last few years, researchers focused on the lipase immobilization on commercial or modified membranes. This procedure shows promising results even after multiple transesterification cycles due to enhanced stability of the immobilized enzyme. Membrane immobilization could be an effective way of enzyme’s activity protection during biodiesel production. This direction of the formulation of biocatalyst could be especially beneficial for the simultaneous transesterification reaction and separation of by-products, as well as for the scale-up of the biodiesel production process. However, in order to enhance the use of immobilized enzymes in industrial processes, more attention is required to the immobilization techniques and operational conditions depending on the specific characteristics of each transesterification process case.