AUTHOR=Maritan Andrew J. , Stewart Frank J. 
  
TITLE=Essential nucleic acid omics: a theoretical foundation for early-stage users
  
JOURNAL=Frontiers in Bioinformatics
  
VOLUME=Volume 5 - 2025
  
YEAR=2026
  
URL=https://www.frontiersin.org/journals/bioinformatics/articles/10.3389/fbinf.2025.1721028
  
DOI=10.3389/fbinf.2025.1721028
  
ISSN=2673-7647
  
ABSTRACT=Modern biology often relies on the analysis of entire sets of molecules (omics). A subset of omics uses nucleic acid sequencing to reconstruct genomes and profile gene expression. Novel findings and existing data are contextualized by databases, which have been growing exponentially due to falling sequencing costs and increased computing access. The increasing accessibility of omics has led to rapid adoption and widespread self-training via open-access tools. In this training environment new users (many of whom are students also applying computing for the first time) are confronted with Terabytes of sequence data and an ocean of topic-specific computing guides (often directed at high-level users). This flood of information creates an initial barrier of confusion and frustration, where it is challenging to identify the overarching goals of omics analyses through the details of computing. We believe this confusion is understandable but not pre-destined, as omics is–at its core–simple. This simplicity comes from its modular nature, where any analysis requires familiarity with only a few consistent steps. Here, we identify core elements of all omics analyses–data products, tools, and workflows–using microbiology applications to ground the discussion. This structure is informed by first-hand experience training early-stage omics users, where covering omics theory provides a foundation for practical implementation.