Front. Bioeng. Biotechnol. Frontiers in Bioengineering and Biotechnology Front. Bioeng. Biotechnol. 2296-4185 Frontiers Media S.A. 1737950 10.3389/fbioe.2025.1737950 Correction Correction: A multi-organ-on-chip to recapitulate the infiltration and the cytotoxic activity of circulating NK cells in 3D matrix-based tumor model Marzagalli et al. 10.3389/fbioe.2025.1737950 Marzagalli Monica 1 Data curation Methodology Visualization Writing - original draft Pelizzoni Giorgia 2 Data curation Methodology Writing - original draft Fedi Arianna 3 Data curation Methodology Writing - original draft Vitale Chiara 3 4 Writing - original draft Fontana Fabrizio 5 Writing - original draft Bruno Silvia 4 Writing - original draft Poggi Alessandro 6 Writing - original draft Dondero Alessandra 4 7 Writing - original draft Aiello Maurizio 3 Writing - original draft Castriconi Roberta 4 7 Writing - original draft Bottino Cristina 4 7 Writing - original draft Scaglione Silvia 3 * Conceptualization Funding acquisition Methodology Validation Writing - original draft Writing - review and editing React4life S.r.l, Genoa, Italy Department of Biotechnology and Bioscience, University of Milano-Bicocca, Piazza Della Scienza, Milan, Italy National Research Council, CNR-IEIIT, Genoa, Italy Department of Experimental Medicine (DIMES), University of Genoa, Genoa, Italy Department of Pharmacological and Biomolecular Sciences (DiSFeB), University of Milan, Milan, Italy Molecular Oncology and Angiogenesis Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy IRCCS Istituto Giannina Gaslini, Genoa, Italy Correspondence: Silvia Scaglione, silvia.scaglione@cnr.it 22 01 2026 2025 13 1737950 02 11 2025 17 12 2025 Copyright © 2026 Marzagalli, Pelizzoni, Fedi, Vitale, Fontana, Bruno, Poggi, Dondero, Aiello, Castriconi, Bottino and Scaglione. 2026 Marzagalli, Pelizzoni, Fedi, Vitale, Fontana, Bruno, Poggi, Dondero, Aiello, Castriconi, Bottino and Scaglione https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. A Correction on A multi-organ-on-chip to recapitulate the infiltration and the cytotoxic activity of circulating NK cells in 3D matrix-based tumor model by Marzagalli M, Pelizzoni G, Fedi A, Vitale C, Fontana F, Bruno S, Poggi A, Dondero A, Aiello M, Castriconi R, Bottino C and Scaglione S (2022) Front. Bioeng. Biotechnol. 10:945149. doi: 10.3389/fbioe.2022.945149 immune-organ-on-chip 3D human tumor model natural killer cells neuroblastoma cells migration cells infiltration section-at-acceptance Tissue Engineering and Regenerative Medicine

A correction has been made to the section Results, Dynamic culture and assessment of natural killer cell “extravasation”, Paragraph 3:

The authors described that they used 40% FBS as a chemoattractant: “To this purpose, additional experimental groups included 1) a positive control of cell migration (“empty” 3D alginate scaffolds, without tumor cells, in culture medium enriched with 40% FBS as a chemoattractant), and 2) a negative control (without alginate scaffolds, with culture medium without any chemoattracting supplement).”

However, the authors used 30%. The corrected paragraph is:

“To this purpose, additional experimental groups included 1) a positive control of cell migration (“empty” 3D alginate scaffolds, without tumor cells, in culture medium enriched with 30% FBS as a chemoattractant), and 2) a negative control (without alginate scaffolds, with culture medium without any chemoattracting supplement).”

A correction has been made to the section Results, Dynamic culture and assessment of natural killer cell “extravasation”, Paragraph 3:

“the stimulation does not report cells “extravasation” events from the bottom chamber towards the tumor, because of the fluid motion alone, as shown in Figure 6C.”

“Figure 6C” should be “Figure 6A.” The corrected paragraph is:

“the stimulation does not report cells “extravasation” events from the bottom chamber towards the tumor, because of the fluid motion alone, as shown in Figure 6A”.

A correction has been made to the section Results, Natural killer cell infiltration, Paragraph 1:

“Figure 6D” is mentioned incorrectly and should be changed to “Figure 6C.” The corrected paragraph is:

“After co-culturing the cells for 4 h under microcirculation, the 3D alginate cultures were recovered, fixed with 4% PFA, NK cells stained for the DNAM-1 marker (Castriconi et al., 2004), and observed under a fluorescence microscopy. As shown in Figure 6C, DNAM-1+ cells were mostly located along the border of the gels. This is indicative of the ability of NK cells not only to specifically migrate toward the tumor culture, but also to infiltrate the alginate matrix.”

The original article has been updated.

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Edited and reviewed by: George Alexander Truskey, Duke University, United States