AUTHOR=Horiguchi Akio , Vaddi Surya Prakash , Rajappa Senthilkumar , Preethy Senthilkumar , Abraham Samuel J. K. 

TITLE=BEES-HAUS preventing urethral stricture recurrence by restoring the integrity of urothelium and its further simplified version, the BHES-HAUS

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 13 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2025.1687741

DOI=10.3389/fbioe.2025.1687741

ISSN=2296-4185

ABSTRACT=Graphical AbstractDiagram illustrating interventional treatment approaches for urethral stricture, emphasizing urothelial integrity in preventing stricture recurrence. It compares procedures such as balloon dilatation and urethrotomy which leave the sub-urothelium exposed to urine leading to inflammation to that of procedures such as BEES-HAUS in which transplanted buccal mucosal cells and in BHES-HAUS transplanted buccal tissue bits, both mixed with Festigel, engraft and restore the urothelial integrity. BEES-HAUS involves a two-week in vitro culture, while BHES-HAUS processes tissue in the operating room in single sitting. Both methods aim for effective urethral treatment using autologous buccal mucosal epithelium.Diagram illustrating interventional treatment approaches for urethral stricture, emphasizing urothelial integrity in preventing stricture recurrence. It compares procedures such as balloon dilatation and urethrotomy which leave the sub-urothelium exposed to urine leading to inflammation to that of procedures such as BEES-HAUS in which transplanted buccal mucosal cells and in BHES-HAUS transplanted buccal tissue bits, both mixed with Festigel, engraft and restore the urothelial integrity. BEES-HAUS involves a two-week in vitro culture, while BHES-HAUS processes tissue in the operating room in single sitting. Both methods aim for effective urethral treatment using autologous buccal mucosal epithelium.