AUTHOR=Al Shalabi Ammar A. , Malik Shaima , Kim Hoon , Alhotan Abdulaziz , Ghoneima Ahmed , Elshazly Tarek M. TITLE=Preclinical evaluation of 3D-Printed orthodontic aligners using an electro-typodont model JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=Volume 13 - 2025 YEAR=2025 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2025.1650447 DOI=10.3389/fbioe.2025.1650447 ISSN=2296-4185 ABSTRACT=ObjectivesThe use of 3D printing in orthodontic aligner production addresses several limitations of conventional thermoforming. However, existing experimental techniques for evaluating aligner efficacy remain restricted. This study aims to introduce a novel experimental approach employing an electric typodont model to assess the effectiveness of 3D-printed orthodontic aligners in correcting rotation of the maxillary right central incisor (Tooth 11).Materials and MethodsAn electric typodont, equipped with heat-activated wax blocks, simulated four rotational severities of Tooth 11: 22°, 32°, 42°, and 52°. Digital scans were processed in Maestro 3D software to design virtual treatment plans, from which four sequential aligners were fabricated per severity level. In total, 240 aligners were 3D-printed, using Tera Harz TC-85 resin, in three thicknesses: 0.50, 0.75, and 1.00 mm. Each aligner underwent a 10-minute heating cycle, followed by a 10-min cooling period. Tooth rotation was measured manually using a protractor relative to a fixed baseline. The procedure was repeated five times per subgroup, with repositioning guided by custom guiding stents.ResultsAcross all aligner thicknesses, 80.0%–93.1% of the planned rotational correction was achieved by the fourth aligner, leaving residual rotations of approximately 4°–5°. Higher initial rotations resulted in a greater percentage of corrections (p < 0.001). The 0.50-mm and 1.00-mm aligners demonstrated faster early-stage correction, whereas the 0.75-mm aligner exhibited a more gradual and consistent derotation pattern throughout the treatment stages (p < 0.001).ConclusionThe electric typodont appears to be a reliable pre-clinical tool for evaluating the effectiveness of aligners. Furthermore, 3D-printed aligners successfully achieved incisor derotation without the use of attachments. Furthermore, while variations in aligner thickness influenced the dynamics of derotation, they did not alter the ultimate correction outcome.