AUTHOR=Li Xiaogang , Zhao Zeqing , Chen Shi , Guo Ye , Pan Hui , Yang Xiao 

TITLE=Quantitative absorptive micro-sampling for decentralized monitoring of insulin-like growth factor 1

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 13 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2025.1648347

DOI=10.3389/fbioe.2025.1648347

ISSN=2296-4185

ABSTRACT=IntroductionThis study establishes a novel quantitative acoustic mass spectrometry (QAMS) methodology for insulin-like growth factor 1 (IGF-1) detection.MethodsChromatographic separation utilized a Peptide C18 column (1.8 μm, 50 mm) with 0.1% formic acid/acetonitrile gradient elution, coupled to tandem mass spectrometry operated in scheduled multiple reaction monitoring (sMRM) mode.ResultsThe method demonstrated a lower limit of quantification (LOQ) of 10 ng/mL with linear dynamic range spanning 10-500 ng/mL. Comparative analysis of 74 paired plasma specimens revealed strong inter-matrix correlation with quantifiable bias.DiscussionThese advancements position QAMS- as a robust tool for decentralized IGF-1 monitoring, particularly valuable in pediatric growth disorder studies and resource-limited settings. Longitudinal stability validation and isoform differentiation remain focal points for future optimization.