With the rapid development of bioengineering technology over the past few years, the research of biomaterials and compounds for promoting tendine-bone healing has also made rapid progress. Biological products such as Calcium phosphate (Cap), Biological nanofiber scaffolds, magnesian products and hydrogel have been widely used in preclinical studies and have achieved good results (Table 1).

Cap material has the advantage of promoting bone formation and has been widely used to enhance bone regeneration. In order to explore the effect of Cap on tendon-bone healing, MUTSUZAKI H et al. (Mutsuzaki et al., 2016; Mutsuzaki et al., 2017) used Cap to soak tendon grafts to try to improve tendon-bone healing. In the treatment of the goat ACL reconstruction model, Cap hybrid tendon grafts showed significant histological improvement in both bone tunnels and increased in-situ force under tibial preload applied at high flexion angles. This research suggests that this technique may promote tendon-bone healing by enhancing osteogenesis. Liao et al. (2021) recently studied the application of amorphous Cap nanoparticles in tendon-bone healing, and found that amorphous Cap nanoparticles couldn’t only improve the biomechanical strength of the tendon-bone junction by promoting osteogenesis, but also promote angiogenesis, thus promoting tendon-bone healing. Secondly, the effects of Ca-5(PO₄) (Moses et al., 2012) SiO₄ (CPS) and hydroxyapatite (HA) on promoting tendon-bone healing were compared. Zhao et al. (2014a) found that the local application of CPS and HA bioceramics both contributed to cell attachment and proliferation, and promoted the formation of new bone. Compared with HA, CPS bioceramics have a more significant effect on tendon-bone healing, which is expected to improve healing after RC injury repair. Strontium (Sr) has good bone conductivity and is widely used as a bone substitute to treat bone diseases. Strontium-enriched calcium phosphate cement (Sr-CPC) was developed by researchers (Kuang et al., 2013). It is found that Sr-CPC has the potential to enhance the fusion of graft and bone tunnel in ACL reconstruction. Preclinical studies have shown that Sr-CPC promotes osteoblast-like cell proliferation, and increases alkaline phosphatase (ALP) activity and the expression of type I collagen, osteocalcin, and osteoprotetin (Panzavolta et al., 2008; Kuang et al., 2012).

In recent years, researchers have attempted to mimic the tendon-bone healing interface through tissue engineering. However, because the tendon-bone healing interface is a transition of soft to hard tissue, the structure is complex. The interfacial connection of tendon-bone healing was observed in different animal models, and it was found that the interfacial connection was mainly composed of Sharpey-like fibers (Tabuchi et al., 2012; Kawakami et al., 2017). Based on this structural property, biological nanofiber scaffolds prepared by electrospinning technology have been developed and widely used in tissue repair of tendon-bone interface (Zhao et al., 2014b; Han et al., 2015). Biological nanofiber scaffolds have the characteristics of good biocompatibility, high aspect ratio and high porosity (Chen et al., 2009; Zhang et al., 2010). In addition, the morphological characteristics of scaffolds play an important role in the adhesion, proliferation and differentiation of active cells (Huang et al., 2016; Mahmoodinia Maymand et al., 2018). Yin et al. (2010) studied the effect of electrospinning nanoscaffolds on the differentiation of human tendon stem/progenitor cells (hTSPCs), and the results showed that nanoscaffolds could induce the tenogenic and osteogenic differentiation of hTSPCs. Cai et al. (2018) prepared a double-layered aligned-random scaffold (ARS) by electrostatic spinning. In the experiment, ARS was used to wrap the Achilles tendon and transplant it through the bone tunnel. The results showed that ARS could effectively enhance the tendon-bone fusion by increasing the area of fibrocartilage and inducing the formation of new bone. Similarly, researchers have experimented with nanoscaffolds loaded with growth factors to promote tendon-bone healing Zhao et al. (2014b) invented an electrospun polylact-glycol ester (PLGE) fiber membrane loaded with bFGF for repairing rotator cuff tear (RCT). The results showed that electrospinning fiber membrane was conducive to cell attachment and proliferation, and accelerated sineoskeletal reconstruction. PLGE fiber membrane loaded with bFGF had a more obvious effect on sineoskeletal healing. In addition, Bi et al. (2021) prepared silk collagen scaffolds loaded with HA and used HA/sericinogen scaffolds as grafts to replace the original ACL in animal models, and the histological staining after surgery showed that a large number of mature bones were formed at the stens-bone interface. Immunohistochemical staining showed more deposition of type I collagen and osteocalcin than that of the control group. These results indicate that the direct use or drug loading of nanoengineered biological scaffolds is a promising method to promote tendon-bone healing.

Magnesium is a biodegradable biomaterial with good biocompatibility and mechanical properties, which can meet the load requirements during knee implantation and active rehabilitation (Farraro et al., 2014). Studies have shown that Mg ion can promote the growth of transplanted tendon into bone through the osteogenic differentiation pathway of stem cells (Yoshizawa et al., 2014). (Cheng et al., 2016) reported the efficacy of biodegradable high-purity magnesium (HP Mg) screw in promoting tendon-bone healing after ACL reconstruction. Research results showed that HP Mg screw can promote the expression of BMP-2, VEGF and glycosaminoglycan in the early stage of tendon-bone healing. Based on the results of this study, magnesium ions are responsible for promoting fibroblast cartilage regeneration by acting on the above molecules. Wang et al. (2017a) compared the efficacy of titanium screw and magnesium screw in promoting tendon-bone healing. The results showed that Mg screw could significantly improve the healing quality of bone marrow tract by promoting the ossification of tendon endothelial area. It was also found that Mg screw can significantly promote the bone formation in the periscrew area at the early stage of healing, and has good corrosion resistance and doesn’t cause bone tunnel enlargement after degradation. Therefore, Mg screw can be used instead of titanium screw in ACL reconstruction. In addition, Wang J et al. found that the release of calcitonin gene-related peptide played a significant role in promoting osteogenic differentiation by activating the cyclic adenosine monophosphate (cAMP) reaction element binding protein pathway, and this effect was significantly enhanced with the increase of magnesium ion concentration in periosteum (Zhang et al., 2016a; Wang et al., 2020). It has also been demonstrated that a higher magnesium ion concentration enhances cell adhesion strength by activating the integrin/local adhesion kinase pathway (Wang et al., 2017b). Cell migration and adhesion are mediated by periosteum proteins through the integrin/FAK pathway. (Qin et al., 2015). Therefore, Mg ions may also affect the secretion of periosteum-derived stem cells (PDSCs). Recent studies have shown that periostein can control bone regeneration potential by up-regulating PDSCs to promote osteogenic differentiation (Duchamp de Lageneste et al., 2018). Wang et al. (2021) used Mg pretreated periosteum wrapped graft tendon, and found that tendon-bone healing was significantly enhanced. These studies suggest that the mechanism of promoting bone growth through Mg ions may be a practical therapeutic strategy to enhance the healing of the tendon-bone interface.

Currently, local injection of bone cement, growth factors and cell transplantation have been identified as potential therapeutic options for promoting tendon-bone healing (Fu et al., 2014). However, how to deliver the drug at the optimal concentration and speed in the tendon-bone healing area remains one of the clinical challenges. Hydrogel microspheres have the advantages of prolonged drug retention time and high drug loading efficiency, and have become a potential excellent drug carrier in the biomedical field. Franklin et al. (2020) demonstrated that hydrogel alone could also promote tendon-bone healing by inducing ADSCs to gather at the tendon-bone interface. Oka et al. (2013) implanted simvastatin coupled gelatin hydrogel into the bone tunnel reconstructed by ACL. It has been found that simvastatin coupled gelatin hydrogel can promote tendon-bone healing through angiogenesis and osteogenesis in the early stage. Similarly, (Lee et al., 2017) confirmed that collagen gel could be used as an effective carrier of BMP-2 to enhance the healing of the tendon-bone interface. Other researchers have combined hydrogels with collagenase for tendon-bone healing. In addition, (Huang et al., 2020a) studied and prepared chitosan/gelatin/β-glycerophosphoric acid (C/G/GP) hydrogel, which was used in combination with collagenase carrier at the tendon-bone junction. The results showed a significant increase in total bone volume in the model treated with collagenase and hydrogel. The above studies indicate that hydrogel alone or as a drug carrier can promote tendon-bone healing, so hydrogel technology may be a promising avenue for the treatment of tendon injuries in the future.

In addition to self-regeneration, Bone marrow mesenchymal stem cells (BMSCs) can differentiate into a variety of cell types, and are widely used in tissue repair and regeneration (Xu et al., 2021). Conventional BMSCs were collected from the pelvis, femur, tibia, and humerus. Some recent studies have shown that BMSCs can be obtained from synovial and adipose tissues, so these tissues have been widely used in tendon-bone repair (De Bari et al., 2001; Hanahan and Weinberg, 2011). Stem cells from different sources have different differentiation ability, and the direction of stem cell differentiation is also regulated by cell factors. Therefore, with the application of combined cytokine and gene overexpression strategies, various types of stem cells have the ability to improve tendon-bone healing (Table 2).

Human umbilical cord blood MSCs (hUCB-MSCs) are used in the study of promoting tendon-bone healing. Jang et al. (2015) studied the safety of non-autologous hUCB-MSCs in animal models of ACL reconstruction and the efficacy of promoting tendon-bone healing. This study found that non-autologous hUCB-MSCs transplantation could be used for ACL reconstruction without early immune rejection. Secondly, more broad fibrochondrocytes were formed and histological scores were higher during tendon-bone healing, and the bone tunnel width was significantly reduced. This study confirms the therapeutic potential of non-autologous hUCB-MSCs in promoting tendon to bone healing. However, it is difficult for hUCB-MSCs to be widely carried out due to ethical factors. Recently, (Tie et al., 2022) compared the efficacy of dedifferentiated osteoblast BMSCs (De-BMSCs) and BMSCs in ACL reconstruction. In this study, De-BMSCs were shown to increase the early biomechanical strength of ACL reconstructions by stimulating bone formation at the tendon-bone interface. The results of this study suggest that De-BMSC transplantation might be more effective for bone formation in the tunnel during tendon repair and reconstruction.

With the increase of stem cell sources, adipose-derived stem cells (ADSCs) have been gradually applied in the study of tendon repair. It has been demonstrated in rabbit ACL reconstruction models that ADSCs can enhance tendon-bone healing (Kosaka et al., 2016) Both histologically and mechanically, local administration promoted the early healing process of the tendon-bone junction. Similarly, (Matsumoto et al., 2021) studied the effect of ADSCs grafts on the biomechanical strength of the tendon-bone interface after ACL reconstruction. The results displayed that ADSCs tablets could enhance the biomechanical intensity of rabbit model and prevent the enlargement of bone tunnel. However, (Kaizawa et al., 2019) found that hydrogel loaded with ADRCs didn’t significantly improve tendon-bone healing after RC repair in a chronic RCT model. Therefore, more studies are needed to examine the efficacy of ADRCs in promoting tendon-bone healing after RCT repair.

Furthermore, significant progress has been made in the identification of clonability, pluripotency and self-renewal ability of tendon-derive stem cell (TSCs) in humans and mice (Bi et al., 2007). Studies have shown that TSCs have stronger clonability than BMSCs (Tan et al., 2012). These cells not only have universal stem cell properties similar to BMSCs, but also feature high expression of genes and proteins associated with tendons (Bi et al., 2007). In recent years, researchers have also explored the effectiveness of TSCs in the repair of tendon-bone injury. Shen et al. (2012) investigated the effectiveness of knitted silky collagen sponge scaffolds seeded with TSCs. After operation, the growth of fibroblasts increased and lymphocyte infiltration decreased at the implantation site of TSCs allogeneic scaffolds. Moreover, the structural and biomechanical properties of the allogeneic TSCs transplantation group were improved. This study demonstrated that the woven silk-collagen sponge scaffolds of allogeneic TPCs improve RC tendon regeneration by differentiating into tendon cells and secreting anti-inflammatory cytokines. Although MSCs are commonly used for tendon-bone junction repair isolated from various tissues, the use of TSCs to repair tendon-bone junctions may be beneficial because the tendon environment is ideal and familiar, facilitating transplantation and differentiation between transplanted cells (Lui and Wong, 2012). The research of TSCs in the treatment of tendon-bone junction injuries is still in its infancy. Further studies are needed to test the efficacy of tspc application in large animal models and to determine its efficacy in vivo.

Researchers have also investigated the effect of human bone marrow stem cells (hBMSCs) on tendon-bone healing. Sun et al. (2019) explored the effect of hBMSCs on tendon-bone fusion in a rat model. The results demonstrated that hBMSCs could accelerate tendon-bone fusion by enhancing fibroblast proliferation, differentiation and collagen synthesis. Similarly, (Chen et al., 2021) examined the effect of hBMSCs on promoting tendon-bone healing in RC repair models. The results of this study show that hBMSCs are capable of promoting tendon-bone healing following RC injuries in rats. Further studies confirmed that the benefits of hBMSCs on tendon-bone healing are related to the regulation of macrophages, and hBMSCs affect the polarization of macrophages through the Smad2/3 signaling pathway. In addition, (Lu et al., 2021) compared Bone marrow mononuclear cells (BMMNCs) and bone marrow stromal cells (BMSC) to promote tenon-bone healing in ACL reconstruction models. In this study, BMMNCs and BMSC were found to be equally effective in promoting tendon-bone healing. These studies suggest that the collection of hBMSCs or mononuclear cells from peripheral blood may also be a potential therapeutic option for promoting tendon-bone healing.

The differentiation ability of BMSCs varies due to different sources. Therefore, the researchers used genetic engineering to modify MSCs to target differentiation to promote tendon-bone healing. Chen et al. (2016) evaluated the effects of BMP2 and bFGF gene modified BMSCs on tendon-bone healing. This study confirmed that BMSCs genetically modified with BMP2 or bFGF can better promote new bone formation, resulting in higher mechanical properties of bone tissue, and thus more effectively promote tendon-bone healing. The study also found that the combination of overexpression of the two genes was more effective than single overexpression therapy. Similarly, (Wang et al., 2018) modified BMSCs by platelet-derived growth factor subunit B (PDGF-B) on tendon-bone healing after RCT repair. This study also found that BMSCs overexpressing PDGF-B could enhance tendon-bone healing after RCT repair. In addition, a recent study by (Kang et al., 2022) found that RUNt-related transcription factor 1 (RUNX1) gene modified MSCs also produced better efficacy in tendon-bone healing. The above studies indicate that genetic engineering technology can make up for the lack of differentiated ability of BMSCs from different sources.

A growing body of research has focused on promoting tendon-bone healing within bone tunnels through the application of cell factors, such as transforming growth factor (TGF), bone morphogenetic protein (BMP), fibroblast factor (FGF), CXCL13, Hyaluronic acid, etc., (Tian et al., 2015; Honda et al., 2017; Lu et al., 2018a; You et al., 2018; Wei et al., 2020). Local application of cell factors has been studied to promote tendon-bone healing and has shown good efficacy in animal models of tendon repair (Table 3).

Transforming growth factor β (TGF-β) family exerts a crucial function in connective tissue development (Kim et al., 2011). TGFβ1 has been shown to promote the formation of fibrous tissue at the site of tendon healing by inhibiting the matrix metalloproteinase pathway (Arimura et al., 2017), and has also been reported to effectively enhance the biomechanical properties of tendons or ligaments (Anaguchi et al., 2005). You et al. (2018) tried to apply TGFβ1-coated HA to repair RCTs, and found that it could significantly enhance the healing of the tendon-bone interface. Further studies showed that the addition of HA-TGFβ1 ceramic powder at the tendon-bone interface could increase the formation of bone and fibrocartilage and improve the collagen structure. Recently, (Zhu et al., 2022a) explored the efficacy of the combination of TGFβ1, insulin-like growth factor 1 and parathyroid hormone in improving the repaired tendon-bone interface. This study found that the combination of cytokine therapy significantly improved the quality of tendon-bone healing and the formation of a mature tendon-bone interface. These studies suggest that TGFβ1 alone or in combination can improve tendon-bone healing, providing a theoretical basis for further clinical application.

BMP-2, a widely distributed glycoprotein in bone matrix, can induce bone formation in vivo by regulating the recruitment and differentiation of bone progenitor cells (Bessa et al., 2008). Tendon-bone healing has been demonstrated to be enhanced by BMP-2 by increasing the biomechanical strength between the two (Ma et al., 2007). Due to its short half-life and structural instability, BMP-2 requires high doses, resulting in adverse effects (Carragee et al., 2011). KIM J G et al.^[82] evaluated the effect of local application of BMP-2 on bone formation and tendon-bone healing. The results showed that BMP-2 alone could promote new bone formation and bone maturation at the tendon-bone interface, thereby improving the biomechanical strength at the tendon-bone interface. In addition, Huang et al. used Collagen sponge ES (CS) combined with BMP-2 to repair bone defects in rats, and the results showed that using CS as a carrier could effectively promote tendon-bone healing (Huang et al., 2015). Similarly, (Wei et al., 2020) studied the effect of CS as a delivery device of OPG/BMP-2 on tendon-bone healing. It was found that the combination of CS and growth factor OPG/BMP-2 can induce the slow and stable release of OPG/BMP-2, and significantly promote the tendon-bone healing in the ACL model.

As members of the FGF family, both acid fibroblast growth factor (aFGF) and bFGF significantly promoted the mitosis of osteoblasts and chondrocytes, and stimulated the formation of new capillaries (Ide et al., 2009; de Girolamo et al., 2015). Therefore, researchers began to try to use aFGF and bFGF to improve tendon-bone healing. Lu et al. (2018a) applied collagen containing different concentrations of aFGF to the tendon-bone interface to evaluate the influence of aFGF/collagen on the healing of the sineoskeletal interface. According to the results, the application of aFGF/collagen composite could promote the early healing of the tendon-bone interface through the growth of new bone, especially the application of high concentration of aFGF/collagen. In addition, (Ide et al., 2009) also demonstrated that local tendon repair with bFGF-treated tendons showed early improvements in bone growth and biomechanical strength at the tendon-bone interface. The results of these studies suggest that FGF may enhance tendon-bone healing, particularly in the early stages after reconstruction.

In the process of cell migration, CXC chemokine ligand 13 (CXCL13) plays a significant role (Zhou et al., 2013). The inflammatory cytokine IL-6 significantly induces CXCL13 expression in human osteoblasts, while CXCL13 expression is not obvious in osteoclasts (Singh et al., 2009), suggesting that CXCL13 may play a role in tendon-bone healing. Tian et al. (2015) evaluated the effect of CXCL13 on tendon-bone healing in rats. The results demonstrated that CXCL13 acted on MSCs through the activation of ERK1/2 signaling pathway by miR-23a, thus promoting tendon-bone healing. Furthermore, hyaluronic acid belongs to the glycosaminoglycan family and is usually found in the synovial fluid of joints, cartilage and other tissues. There is evidence that HA plays an important role in repairing tissue damage (Neuman et al., 2015). Honda et al. (2017) explored the effect of hyaluronic acid on tendon-bone healing after RCT repair. It was found that hyaluronic acidon accelerated tendon-bone healing, enhanced biomechanical strength, and increased chondroid formation and tendon maturation at the tendon-bone interface in tendon repair models. These studies suggest that both CXCL13 and hyaluronic acidon can promote tendon-bone healing by activating the MSCs pathway, and they are also potential candidate cell factors for promoting tendon-bone healing.

Because many cell factors are involved in tendon repair, cell factors are widely used in tendon repair. PRP, as a source of cell factors, has been shown to improve inflammation, promote cell proliferation, and enhance angiogenesis (Lang et al., 2018; Santos et al., 2018). PRP has also been identified as a potential therapy for accelerating tendon repair. Studies on the application of PRP in the repair of soft tissue wounds have confirmed its efficacy in promoting the healing of tissue wounds (Table 4). PRP is involved in tissue remodeling, chondrogenic differentiation, and wound healing by releasing cell factors (Mehrabani et al., 2019).

There have been numerous studies investigating the efficacy of PRP in reconstructing the ACL and repairing the RCT. Ağır et al. (2017) evaluated the effect of PRP on tendon-bone healing in ACL reconstruction. The results demonstrated that the use of PRP during tendon implantation into the bone tunnel could promote tendon-bone healing by inducing fibrochondral formation early on. Besides, in order to optimize the release of PRP in the joint, the role of PRP combined with drug carriers in promoting tendon-bone healing was also explored.

Zhang et al. (2019a) explored the effect of PRP combined with gelatin sponge (GS) on tendon-bone healing. It has been shown in vitro that GS-loaded PRP can prolong its bioactivity time and promote BMSC proliferation. PRP promotes early healing of tendon-bone junctions in a model of ACL reconstruction. However, (Ersen et al., 2014) previously found that PRP improved the biomechanical properties of RC tendon-bone interface regardless of whether GS was applied. The different models used in the two studies may account for the different results. PRP in combination with other biomaterials has also been studied to promote tendon-bone healing. Graphene oxide (GO) can promote tissue repair by improving the physical properties of tissues. Bao et al. (2021) prepared PRP gel containing different concentrations of GO to promote tendon-bone healing in rabbit tendon reconstruction model after RC injury. The results demonstrated that the addition of GO enhanced the biomechanical properties of the newly formed tendons and made the tendon-bone interface more stable. Therefore, the combination of PRP and GO has great potential in the treatment of RCT. In addition, platelet-rich fibrin (PRF), as a concentrated product of PRP, has also been used in the study of promoting tendon-bone healing. A recent study by (Uno et al., 2022) found that PRF significantly enhanced tendon-bone continuity in the RCT model, and at the same time achieved better tendon-bone maturation. Consequently, PRP and its concentrate have great potential to promote tendon-bone healing.

In recent years, more and more evidence shows that BMSCs are effective in the treatment of joint diseases (Zhang et al., 2019b). BMSCs are involved in many regulatory functions through the paracrine capacity of exosomes (Kourembanas, 2015). Exosomes are bilayer extracellular vesicles containing small RNAs and proteins and are considered to be important mediators for genetic exchange and communication between cells. Recently, researchers have found that stem cell-derived exosomes can improve the healing rate of tendon repair (Zhang et al., 2021). Therefore, many studies have been carried out on exosomes in promoting tendon-bone healing, providing more theoretical basis for promoting tendon-bone healing (Table 5).

The number of studies on exosomes that promote tendon-bone healing has increased significantly in recent years. Huang et al. (2020b) Xu et al. (2022a) explored the role of bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exos) in tendon-bone healing after RC and ACL reconstruction respectively. The results confirmed that BMSC-Exos can increase angiogenesis and bone growth at the tendon-bone interface, which may be related to immune regulation of macrophage polarization. Further studies by (Shi et al., 2020) found that local administration of BMSC-Exos promoted the formation of fibrocartilage by mediating the polarization of M2 macrophages, thus improving the biomechanical properties of the tendon-bone interface. In addition, LI Z et al.^[117] observed the effect of BMSCs-Exos on ACL reconstruction posterior tendon bone healing and its possible mechanism in vitro and in vivo. According to the results, BMSC-Exos promoted the polarization of M1 macrophages to M2 macrophages through miR-23a-3p, reduced the early inflammatory response at the tendon-bone interface, and promoted early healing after ACL reconstruction. BMSC-Exos could be used in the repair of tendons and bones based on the findings of this study. In conclusion, BMSCs-Exos has been shown to be an effective and viable natural drug for enhancing tendon-bone healing, suggesting that BMSCs-Exos may be a promising therapeutic strategy.

In recent years, non-invasive physical therapy has been gradually applied to promote tendon-bone healing. Low intensity pulsed ultrasound (LIPUS), as a non-invasive physiotherapy technique, has been shown to promote recovery from musculoskeletal injuries. LIPUS transmits high frequency acoustic pressure waves and mechanical stresses through the skin to biological tissues. It has been successfully demonstrated in animal models and clinical trials to stimulate bone growth and thus promote fracture healing (Hannemann et al., 2014). In addition, it has been shown that extracorporeal shock wave (ESW) can promote tendon-bone healing by upregating the expression of fibrochondrogenic cell factors through mechanical signaling. LIPUS and ESW, as non-invasive physical therapy, have also been studied in the field of promoting tendon-bone healing, and have achieved good efficacy (Kuo et al., 2007; Greve et al., 2009) (Table 6).

In 2013, Lovric et al. (2013) conducted the first study to test the effect of LIPUS on the initial tendon-bone healing in RC sheep model. The results suggest that LIPUS may help RC repair in the initial stages of tendon-bone healing. Afterwards, LU H’s team conducted a large number of studies in this field (Lu et al., 2016a; Lu et al., 2016b; Lu et al., 2018b). First, they determined the optimal time for LIPUS treatment, and the study found that LIPUS treatment on the 7th day after surgery had a more significant impact on tendon-bone healing. Further studies showed that LIPUS accelerated bone formation during tendon-bone healing from the second week after treatment, significantly improving the quality of tendon-bone healing. Finally, they evaluated the dose-effect of LIPUS stimulation on tendon-bone healing. The study found that LIPUS stimulation twice a day was better for muscle and bone healing than the treatment once a day. Recently, (Xu et al., 2022b) observed in rats RC tear model that macrophage polarization may be a potential mechanism for LIPUS treatment of TBI repair. In conclusion, LIPUS therapy, as a non-invasive physical therapy, has been thoroughly studied in terms of promoting the mechanism of tendon-bone healing and the selection of treatment time and frequency. These results indicate that LIPUS therapy, as a technique to promote tendon-bone healing, may be widely used in clinic.

ESW is another potential non-invasive form of physical therapy that promotes tendon-bone healing. Chow et al. (2014) explored the effect of ESW on fibrocartilage regeneration during tendon-bone healing. It was found that ESW upregulated the expression of cell factors related to fibrocartilage production by providing mechanical signals, thus accelerating fibrocartilage regeneration at the healing interface. More studies are needed to confirm the efficacy and mechanism of ESW in promoting tendon-bone healing. In addition, intermittent negative pressure and Pulsed electromagnetic field (PEMF) therapy have been preliminarily explored to promote tenoon-bone healing after tendon repair with positive results (Sun et al., 2017; Huegel et al., 2022). More research is needed to confirm their specific efficacy and mechanism.

In recent years, the physiological mechanism of tendon-bone healing has gradually become clear. Researchers are also trying to combine these methods to enhance their efficacy in promoting tendon-bone healing after tendon injury. Stem cells, cell factors, PRP and biomaterials were used in combination with each other, and positive effects were achieved (Table 7).

At present, stem cell and PRP therapy techniques are the mainstream methods to promote tendon-bone healing. The combined application of the two is also widely used in this field. Teng et al. (2016) studied the ability of PRP combined with BMSCs to promote tendon-bone healing. Studies have shown that PRP-BMSCs promote more mature healing of the tendon-bone interface, and the joint shows a higher functional load. Similarly, (Han et al., 2019a) explored the efficacy of combined MSCs and PRP in RC damage repair model. The results showed significantly enhanced signals related to angiogenesis, bone formation, and in situ tendon formation induced by combination therapy. The above studies confirm that the combination of MSCs and PRP can synergistically promote tendon-bone healing, which has great hope for promoting the treatment of tendon-bone healing. In addition, (McGoldrick et al., 2017) prepared extracellular matrix hydrogel loaded with PRP and ADSCs in order to make the drug release in the joint more lasting and better. It showed superior repair ability and biocompatibility in the rat model of Achilles tendon injury repair. Therefore, optimizing the effect of drug release based on the combination of stem cells and PRP may offer greater hope for promoting tendon-bone healing.

It was found that the combination of MSCs and demineralized bone matrix (DBM) can also enhance the role of MSCs in promoting tendon-bone healing. Thangarajah et al. (2016) explored the effect of DBM combined with MSCs on improving the function of the tendon-bone interface. In the treatment of patella tendon injury repair model, it was found that the combination effectively promoted the healing of the tendon-bone interface. Similarly, (Thangarajah et al., 2018) also studied the effect of the combination of DBM and MSCs on RCT healing. The results also showed that the combination of the two methods enhanced RC healing at an early stage and restored bone mineral density in the RC to pre-injury levels. These studies suggest that tendon repair using DBM and MSCs can achieve better tendon-bone healing early after surgery, and further studies are needed to determine its efficacy.

Furthermore, researchers have provided a new strategy for promoting tendon-bone healing. Combining biological scaffolds with tissue-specific stem cells may be a promising protocol for promoting tendon-bone healing. Hu et al. (2018) explored the synergistic therapeutic effect of collagen-filament scaffolds combined with derived stem cells on ACL regeneration. It was found that this treatment strategy significantly improved cartilage degeneration and reduced the severity of joint fibrosis. Similarly, (Chen et al., 2020) developed polylactic acid-glycolic acid (PLGA) scaffolds loaded with BMSCs. In a rabbit RCT repair model, it was found to promote tendon-bone healing by improving the biomechanical properties of the regenerated tendon. Besides, (Chen et al., 2019) studied the efficacy of scaffolds loaded with gene-edited BMSCs in improving RC repair. BMP-12 overexpressed rabbit BMSCs were used in this study. The results demonstrated that the PLGA scaffold loaded with BMSCs overexpressing BMP-12 could promote the healing of the tendon-bone interface, improve collagen tissue and increase fibrocartilage in rabbit RCT repair. In conclusion, biological scaffolds combined with MSCs are an effective strategy to improve tendon-bone healing, especially the combined use of MSCs that can be directed differentiation after gene modification.