AUTHOR=Serrano-Jara Daniel , Agea Iván , Romero Gema , Argente María José , Santacreu María Antonia , García María de la Luz 

TITLE=Genetic correlated responses to selection for resilience in key inflammatory and stress biomarkers under heat stress of rabbits

JOURNAL=Frontiers in Animal Science

VOLUME=Volume 6 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/animal-science/articles/10.3389/fanim.2025.1694508

DOI=10.3389/fanim.2025.1694508

ISSN=2673-6225

ABSTRACT=Heat stress is a major challenge for rabbit production, due to the species’ limited thermoregulatory capacity. Two rabbit lines were divergently selected over 17 generations for environmental variance in litter size: the Low line, with greater resilience, and the High line, with less resilience. This study aimed to compare acute stress and inflammatory responses in males from both lines under heat stress (temperature–humidity index, THI ≥ 27.8) and thermoneutral (THI< 27.8) conditions. Forty males (20 per line) were evaluated for eyeball temperature via infra-red thermography before and after a semen collection stressor, and for plasma cortisol, C-reactive protein (CRP), and tumor necrosis factor-α (TNF-α) by ELISA. Bayesian methodology was used for statistical analysis. The Low line maintained lower eyeball temperatures than the High line under both thermal conditions (P > 90%), while retaining normal acute stress responses. Under heat stress, the Low line also showed lower TNF-α levels, and under both conditions, lower cortisol levels (P > 90%). CRP did not differ between lines or thermal conditions (P< 90%). These results indicate that selection for reduced litter size variability improves thermal regulation and attenuates stress-associated physiological responses, supporting its use to enhance resilience, welfare, and sustainability in rabbit production.