A number of low-glycoalkaloids potato cultivars were developed by Rumafeed (Mishmar HaShivaa, Israel) using CRISPR/Cas9 gene editing technique (Johansen et al., 2019) for precise knock down of genes coding for GAs synthesis in the tetraploid plants (Krits et al., 2007; Sawai et al., 2014). Following tissue culturing, hardening (Mohapatra and Batra, 2017) and cultivation in a closed air-conditioned greenhouse, and MS-GC assays for GAs, post-storage tubers from selected mutants were planted and plants grown for more than 100 d in 7 L pots containing standard growing medium. Standard agricultural management was practiced throughout. Following dehaulming a fortnight prior to tuber harvesting, the fresh cut haulm was dried at 60°C for 48 h in an air-forced oven to make LGPH hay which is then stored in a polythene bag for experimental diet formulation.

Using total mixed ration (TMR, Nahlal feed center, Nahlal, Israel) and pellets (25.5% CP #16100; Ambar feed mills, Hadera, Israel), combined with LGPH hay, the experimental diets were formulated to satisfy the nutritional requirements for small ruminants as outlined by the national research council (National Research Council, 2007). The constituents of TMR and the chemical composition of the ingredients used for dietary formulation are presented in Table 1 .

Control diet (CON) consisted of a maintenance diet made of TMR and pellets (ingredients are presented in Table 1 ) without addition of LGPH. Whereas treatment diets were formulated by addition of 10%, 15%, and 20% LGPH to make diets P10, P15, and P20, respectively on a DM basis ( Table 2 ). All ingredients (TMR, pellets, and LGPH) diets were mixed manually by hand every morning to create the respected dietary treatments. The particle size distribution of the experimental diets was determined using a Penn State Particle Separator (PPS) with a 19-mm screen (Upper), an 8-mm screen (middle), a 4-mm screen (lower) and a pan (fine) as described by Lammers et al. (1996).

The experiment was approved by institutional ethics committee of the Hebrew University of Jerusalem (AG-16738) according to regulations of animal use for scientific purposes-directive 2010/63/EU and Israeli law.

The study was conducted at the metabolic experimental farm located at The Robert H. Smith Faculty of Agriculture, Food, and Environment, Rehovot campus (FAHU). The experimental environment was carefully controlled to maintain an ambient temperature between 21 and 25°C, relative humidity levels between 55 to 60% and a 12-hour light/dark cycle daily.

Four Asaf female lambs (4 months old) were purchased from a local trustworthy farmer in Israel and transported to the experimental facility at the FAHU. Upon arrival the facility, the sheep were dewormed and vaccinated according to the recommendations of Israeli Ministry of Agriculture and Rural Development and then allowed 1 month of acclimatization before the start of experiment.

The average sheep body weight at the start of the experiment was 44.1 ±1.7 kg. Each sheep was housed in an individual metabolic cage (1.5m x 0.8m) equipped with a drinker to provided ad libitum access to water, as well as an automatic feeder (Ankom Technology, Macedon, NY) set to deliver 12 equal meals per day. Each sheep was offered additional feed allowance to reach up to 10% refusal. A hard plastic net underneath each cage was used for collection of faecal samples.

The study utilized a 4 × 4 Latin square design with four experimental periods, each lasting a total of 21 days, subdivided into 14 days of adaptation followed by 7 days of sampling. During the adaptation period, sheep were put down in a free stall yard to socialize and stretch for a period of 3-4 hours every day. The sheep were randomly and concurrently assigned to one of the four experimental diets: CON, P10, P15, and P20 ( Table 2 ). The dietary treatments were rotated after every 21 days while ensuring that each treatment was allocated once per row and column in the Latin square matrix. During the research, the animals were routinely monitored by a veterinarian for any potential clinical signs of GAs poisoning and other health problems. The lambs were weighed at both the start and end of each experimental period to monitor the average daily weight gain associated with each experimental diet. However, it is crucial to acknowledge that the Latin square design used in this study has its limitations because of the short duration. Therefore the experiments results do not comprehensively describe growth performance compared with continuous design (Hristov et al., 2019).

The digestibility experiment was performed according to Omar (2002); Al-zubiadi (2016) and Al-Baadani et al. (2022). During the 7 days of sample collection, the daily amount of feed offered and refused for each animal was recorded, and the daily dry matter intake (DMI) was calculated. Feed samples (both the diet offered and the refusals), and faeces for each sheep were collected daily, weighed, and stored in a labelled plastic bag at -25^oC for chemical analysis.

On the 6^th day, an hour after the first morning feeding, ≈50 mL of rumen liquid was collected using a vacuum pump (MRC, Holon, Israel) connected to an oro-ruminal tube (VetMarket, Modiin, Israel). To avoid contamination with saliva, the first 50 ml were discarded, and the ruminal tube was inserted to reach the ventral region of the rumen at a predetermined tube length. The pH of the rumen fluid was measured immediately using a pH meter (Satorius Ag-Gottingen, Germany). The concentration of NH₃-N in rumen fluid was determined using a spectrophotometer after sample preparation by reacting ammonia with alkaline hypochlorite and phenol in the presence of sodium nitroprusside as a catalyst to create indophenol (Bower and Holm-Hansen, 1980).

Concomitantly, blood samples were collected after the first morning feeding through jugular puncture and quickly transferred into vacutainers (one with EDTA and another with clot activator). The blood samples were then immediately sent to Hebrew University Veterinary Hospital (Bet-Dagan, Rishon Letsiyon, Israel) for quantification of haematological and biochemical parameters using an autoanalyzer spectrophotometer (Roba et al., 2022).

On the last day of every experimental period, urine sample was collected using catheters and stored in urine bags (1.5 Litres; VetMarket, Modiin, Israel) filled with 20 mL of 10% sulphuric acid, (Foley catheter 16 Fr.; Degania Silicone Ltd., Hatzor HaGlili, Israel). The urine excreted by each animal over the duration of 24 hours was pooled, mixed thoroughly, recorded, and 150 mls sample/sheep were stored at -25˚C for determination of urinary nitrogen excretion and energy loss.

At the end of each sampling period, the faeces, feeds, and refusals from each animal were thawed, pooled per animal, thoroughly mixed, and 10% (w/w) of the total samples were separately dried in an air-forced oven at 60°C for 48 h. The samples were weighed and ground to pass through a 2 mm screen using a knife mill (Thomas–Willey Laboratory Mill, model 4, Arthur H. Thomas Company, Philadelphia, PA, USA), and then stored in labelled paper bags awaiting analysis.

The chemical analyses were conducted according to AOAC methods (1980). Samples were dried overnight at 105°C in an air-forced oven (WTC Binder, Germany) to measure their dry matter (DM) content. Subsequently, the dried samples were burned in a muffle furnace at 600°C for 5 h to determine their organic matter (OM) content. The ether extract (EE) was quantified by extraction in a Soxhlet apparatus for 6 hours using petroleum ether (40-60˚C; Merck, Rehovot, Israel). The crude protein (CP) content was determined using an automatic Kjeldahl machine (KjelMaster K-375-BUTCHI, Switzerland) and starch was quantified by an enzymatic-colourimetric method (Hall et al., 2015). Neutral detergent fiber (NDF), acid detergent fiber (ADF), cellulose and lignin measurements were performed using the Ankom fiber analyzer (Ankom²²⁰ Technology, Macedon, NY) in accordance with Van Soest et al. (1991). A bomb calorimeter (6100, Parr Instrument Company, Moline, IL) was used to measure the gross energy (GE) of samples of experimental diets, refusals, feces, and urine. Benzoic acid was used as the reference standard. Urinary energy was performed on dried urine sample with the aid of paraffin mineral oil. Volatile fatty acids (VFAs) concentration in the rumen liquid samples were determined by gas chromatography (Erwin et al., 1961). Metabolic energy (ME) and combustible gas energy (GasE) were estimated in accordance with the National Research Council recommended protocol (NRC, 2001; National Research Council, 2007).

The daily dry matter intake (DMI) was calculated on a DM basis using formula: DMI = (amount of feed offered–refused feed). The apparent digestibility coefficient of nutrients was computed as the difference between the nutrients consumed in the diet and those excreted in faeces, expressed as a percentage of intake.

Data for daily dietary intake, nutrient digestibility, ruminal fermentation parameters and blood parameters were analysed using ANOVA on JMP Pro software (SAS Inc.), (2016) employing the Latin square design with diet and period as fixed effects and sheep as random effect. The statistical differences among the diets were determined using Tukey’s Honestly Significant Difference (HSD) for all pairwise experimental diet comparisons. The level of significance was established at P< 0.05. Furthermore, orthogonal contrasts were used to detect potential quadratic effects of LGPH inclusion in the experimental diets. Accordingly, respective equations (either linear or quadratic) were derived and used to estimate (extract) ME and nutrient digestibility of LGPH (Fonnesbeck et al., 1981).

After estimating the ME and GasE (NRC, 2001; National Research Council, 2007), metabolic energy concentration (Mcal/kg DM) was calculated as the ME intake (MEI) divided by its respective DM intake (DMI).

Freshly harvested LGPH has 9.27% DM content, and its chemical composition is shown in Table 1 . All the experimental diets showed a similar composition of OM, ash, NDF, ADF, hemicellulose, cellulose, lignin, EE, and starch, whereas the DM and CP content increased linearly with the addition of LGPH ( Table 2 ). Thus, demonstrating the high nutritious quality of the haulm.

The inclusion of LGPH had no effect on particle size distribution in the experimental diets ( Table 3 ). Yet, there was a slight variation in the proportion of DM retained on the different sieves of PSPS. On average, the 4-8 mm particle size constituted the highest DM proportion (29.1 ± 2.32%) followed by 8-19 mm and > 19 mm particle sizes respectively, while the< 4mm particle size constituted the lowest DM proportion (21.7 ± 1.89%) on average.

The daily dietary intake of lambs fed on LGPH dietary treatments is shown in Table 4 . The addition of LGPH had no effect on the daily intake of DM, OM, NDF, EE, starch, GE, and DE. Furthermore, LGPH inclusion in the experimental diets had a significant increase (P= 0.001) in daily CP intake, with diet P20 showing the highest CP intake at 281.7 g/day ( Table 4 ). These findings provide additional evidence that potato haulm possesses a high level of nutritional quality.

The study further examined the apparent digestibility of nutrients in diets containing different levels of LGPH ( Table 4 ). The apparent digestibility of ADF showed a linear tendency, while that of DM, OM, CP, and GE in lambs increased (P< 0.05) with the LGPH level in the diets. Hence the DM and CP levels of digestibility were 64.7% and 70.6%, respectively for P20, compared with 61.3% and 63.2% and in the control.

Additionally, LGPH had a quadratic effect on the NDF digestibility, with a peak digestibility (54.7%) occurring at 12.3% LGPH in the diet but had no significant effect on the EE, starch, and ADF digestibility. Furthermore, there was no significant differences in ME concentration among the experimental diets. Once again, our findings demonstrate the nutritional advantages of potato haulm.

Orthogonal contrasts were employed to determine whether the effects of LGPH on the diet were linear or quadratic. Table 5 displays the computed digestibility of DM, OM, CP, NDF, and energy as well as metabolizable energy of LGPH using the linear or quadratic equations.

The equations for dry matter digestibility (DMD), organic matter digestibility (OMD), crude protein digestibility (CPD), gross energy digestibility (GED), and metabolizable energy (ME) displayed a linear relationship, while the equation for NDF digestibility (NDFD) exhibited a quadratic pattern, as follows:

ME = metabolizable energy calculated based on gross energy of LGPH, and NRC equations (NRC, 2001; National Research Council, 2007). NDFD was extracted at proportion of LGPH (12.3%) in the diet which produced the maximum diet digestibility (54.8%) from the derivative equation. Therefore, supplementing diets with LGPH can potentially improve their nutritional value due to its excellent metabolizable energy and nutrient digestibility.

The addition of LGPH to the experimental diets increased the daily N intake linearly (P = 0.001), with diet P20 showing the highest intake of 44.5 g/day compared to 33.6 g/day in control diet ( Table 6 ). Furthermore, a corresponding) rise (P< 0.05 in N retention and the ratio of N retention to N intake were observed, with the diet P20 exhibiting the highest values of 19.1g/d and 0.42, respectively. However, dietary supplementation with LGPH had no significant effects on faecal and urinary nitrogen excretion. Therefore, the inclusion of LGPH in diets can lead to an enhanced efficiency of nitrogen utilization.

We analysed rumen liquids to determine whether addition LGPH to experimental diets affected the rumen fermentation parameters. It was noted that LGPH supplementation had no significant effect on pH, NH₃-N, acetic (C2), propionic (C3), butyric (C4), caproic acid (C6) acids, C2:C3 ratio, and total VFA concentration ( Table 7 ). Although the addition of LGPH had no significant effect on total VFA concentration, there was a tendency of a rise in total VFA concentration with increasing levels of LGPH in the treatment diets. However, it is important to highlight that addition LGPH to the treatment diets resulted in a significant (P< 0.05) decrease in levels of isovaleric (Ci5) and valeric (C5) acids in the rumen of the lambs. Therefore, LGPH supplementation maintains the integrity of rumen parameters appears to increase the concentration of total VFAs.

The results ( Table 8 ) show that the LGPH incorporation in sheep’s diet led to an increase in the concentrations of total proteins (TP) and blood urea nitrogen (BUN) in sheep blood. Diet P15 showed a maximum TP level of 6.49 g/dL, whereas diet P20 exhibited the highest BUN concentration of 39.3 mg/dL compared with 5.22 g/dL and 26.6 mg/dL in the control, respectively.

The dietary treatments had no effect on the levels of hepatic enzymes and the various blood parameters that were assessed in this study. The average body weight of the lambs increased from 44.1 ±1.7 kg at the beginning of the trial to 51.0± 2.4 kg at the end of the trial. Routine check by veterinarian didn’t observe any adverse clinical manifestation. Therefore, by maintaining blood biochemistry within the normal physiological range, it can be inferred that LGPH supplementation does not have any detrimental effects on the health of sheep.

In comparison to other potato varieties (Dao and Friedman, 1996; Zarzecka et al., 2013), the LGPH has very low levels of alpha-solanine (0.1-2.5 µg/g) and alpha-chaconine (0.4-5.5 µg/g) versus ~500 mg/100 g reported by (Friedman and Dao, 1992). The LGPH lines employed in our studies were developed using gene-editing techniques to target and knock down the genes which code for potato GAs (Krits et al., 2007).

Assessing the chemical composition of forage plants is critical for establishing their usefulness in animal nutrition (Assefa and Ledin, 2001). This study suggests that LGPH has high-potential application in ruminant diet formulation. Our results show that LGPH has higher CP and ash content but lower NDF and ADF content compared to other potato genotypes (Kaplan et al., 2018) studied in Turkey. This can possibly be attributable to differences in genotypes, physiological age, and growing environment (Nicholson et al., 1978; Maga, 1980; Jadhav et al., 1981; Assefa and Ledin, 2001; Kaplan et al., 2018). Nicholson et al. (1978) reported that the CP content in potato vines varies between 8-21% but decreases with prolonged harvest periods. The high CP content (26.3%) in the LGPH could have resulted from not only genetic and environmental factors but also maturity stage. This quality makes LGPH varieties exceptional since protein is one of the most expensive and limiting factor in ruminant nutrition (Parisi et al., 2020). The linear rise in the CP content noted in the treatment diet possibly resulted from the dietary supplementation with LGPH which has high CP content. Additionally, it’s worth noting that the addition of LGPH hay contributed to the observed linear increase in the DM content of the experimental diets which is in agreement with the earlier report by Koli et al. (2019).

This study shows that the LGPH genotypes employed have higher nutrient digestibility compared with potato haulm from other cultivars (Parfitt et al., 1982; Kaplan et al., 2018). The high digestibility noted in this study could be explained by low NDF, ADF and lignin in the LGPH compared other cultivars. High levels of NDF and ADF in the diet is known to slow down digestion but merely give animals sensation of physical fullness and satiety (Riaz et al., 2014). This occurs when high forage diets accumulate in the reticulo-rumen, triggering the stretch receptors on its muscular wall to signal satiety regions of the brain and cause a feeling of fullness (Allen, 2000). Furthermore, it is also possible that the nutrient digestibility in the present study was not affected by the GAs since LGPH had negligible GAs concentration. Previous studies have indicated that GAs can disrupt the integrity of the digestive system (EFSA, 2020). Therefore, LGPH exhibits not only exceptional nutritional composition but also high nutrient digestibility, making it a desirable quality for forage.

Our results show that LGPH was acceptable by the sheep and didn’t affect intake of DM, OM, NDF, EE, starch, and energy in the experimental diets. These findings supports Parfitt et al. (1982) who studied the nutritional value of pressed potato vine silage and reported uniform voluntary intake in goats. This observation in the current study could be because the experimental diets were well-blended with a uniform particle size distribution, which resulted in a consistent palatability of the diets and reduced feed selection by the lambs. It is worth considering that the blocking of GAs biosynthesis also resulted in the absence of GAs-associated bitter sensations in LGPH thus contributing to the normalcy in the voluntary feed intake in the treatment diets (Baumont, 1996; EFSA, 2020).

The increase in CP intake is attributed to the corresponding linear rise in CP content in the experimental diets. This consequently improved nutrient (DM, OM, CP) and energy digestibility, owing to the stimulatory effect of CP on dietary intake and nutrient utilization (Silanikove et al., 1997). Moreover, supplementing protein from foliage has been shown to improve forage digestion (Bodine et al., 2000; Aregheore and Perera, 2004). It is also possible that the higher CP intake resulting from LGPH inclusion synchronized better with nutrient availability, ultimately leading to optimal rumen fermentation and microbial growth (Tripathi et al., 2007). Therefore, the increased digestibility observed in the treatment diets compared to control could have resulted from contribution of LGPH as a supplemented component (Patra, 2010).

The incorporation of LGPH in the treatment diets resulted in a quadratic increase in NDF digestibility, peaking at 54.8% when LGPH was included at a proportion of 12.3% in the diet. The increase in NDF digestibility could be attributed to the optimal degradability of NDF by the rumen microbiomes (Lavrenčič et al., 1997). However, when LGPH was added above 12.3%, NDF digestibility decreased probably due to a reduction in ruminal mat formation and an increased ruminal passage rate (Patra, 2010). Waldo et al. (1972) proposed a compartmental model to calculate digestibility of potential digestible nutrients (PD) as follows: Digestibility = PD × [kd/(kd + kp)]where; k_d = the rate of digestion, and k_p = rate of passage.

It is generally suggested that an increased k_p limits the extent of digestion of potential digestible components of feeds (Waldo et al., 1972). This could explain the decrease in NDF digestibility observed when LGPH was added above 12.3%, as the high LGPH levels may have led to an increased ruminal passage rate, limiting the extent of NDF digestion. To maximize the digestibility of NDF, the inclusion of LGPH in the diet should be optimized in conjunction with coarse roughages and carefully regulated within appropriate limits.

A synchronized balance between nitrogen and energy availability in the rumen is crucial for attaining maximum microbial protein production, efficient ruminal fermentation, and improved feed utilization in livestock (Cabrita, 2006). Evidently, excess N in the rumen is not utilized for microbial protein production but is added to the ammonia pool, which is then transported to the liver, converted into urea, and largely excreted in as urine (Patra, 2010). This was not the case in the present study where the levels of ruminal NH₃-N and urinary N excretion were not affected by dietary treatment. Hence, the inclusion of LGPH in the diets resulted in an increase (P< 0.01) in N retention (g/day), primarily due to higher CP intake and increased CP digestibility, implying optimal level of amino acids are supplied to match physiological requirements of sheep.

The increase in N retention (NR) in animals could have resulted from optimizing the protein-energy balance (P/E) in the treatment diets (Setyono et al., 2022). Consequently, efficiency of N utilisation, expressed as the ratio of N intake to N retention correspondingly exhibited a linear increase. Similar linear increase in N retention upon inclusion of high CP soya hulls in the forage diets response was reported by De Araujo et al. (2008).

Our results show a positive P/E balance that could potentially enhance productivity, particularly weight gain, as reported by Setyono et al. (2022). The metabolizable energy values recorded in the current study are higher than those reported by Kaplan et al. (2018). This dissimilarity could be attributed to the difference in methodologies applied. The current study was conducted in vivo, whereas the former used 24hr gas production and chemical composition to calculate metabolizable energy. Furthermore, the high energy values reported in our study could be due to the physiological age of the sheep, which were young and fast growing (Costa et al., 2013). As sheep grow at high rates, their basal metabolic rates increase, stimulating and enhancing the activities of both the cardiovascular and digestive systems to digest, absorb, and supply the nutrients necessary for their rapid growth (Yang et al., 2020). Our results demonstrate the beneficial effects of adding LGPH to experimental diets, as it improves both N retention and energy balance in lambs.

Rumen pH, NH3-N, and VFA concentrations are key parameters that reflect the microbial growth and fermentation processes occurring in the rumen. These parameters can be influenced by various factors, such as dietary intake, composition, degradability, fermentation and absorption rate as well as synchrony of available N and energy in the diets (Chumpawadee et al., 2006). To maximise the efficiency of microbial protein synthesis the diets should provide optimum rumen conditions (Cabrita, 2006). For instance, rumen pH as the main variable of fermentation has to remain within the critical range of 6.0-7.0 for optimal nutrient utilisation and microbial growth (Soto et al., 1994). In the current study, rumen pH was not affected by addition of LGPH in the diets and varied between 6.44 and 6.59 probably due to the fact that the diets were mainly made of forage with low proportion of quickly fermentable non-structural carbohydrates that might cause a rapid decline in the rumen pH (Ryle and Ørskov, 1990).

Additionally, VFAs as weak acids that dissociate and release protons in the rumen, potentially contribute to lowering and stabilizing ruminal pH by either eliminating protons via passive diffusion or by secreting bicarbonate via anion exchange pathways (Penner, 2014). Primarily VFAs are synthesised through the fermentation of carbohydrates, as well as by the partial breakdown of proteins and cleavage of lipids (Owens and Basalan, 2016). It is also possible that production of VFAs didn’t exceed the buffering capacity of the saliva and bicarbonate ions (e.g., rumination activity). The lower concentration of total VFAs in our study compared with Ramos et al. (2009) report could be attributed to the difference in the experimental design and timing of rumen liquid collection, as our collection was done in a semi steady-state metabolic conditions, whereas their collection was done two hours after feeding.

Bergman (1990) reported that 75% of metabolizable energy originates from VFAs. Therefore, diets that promote fermentation and VFAs production, also promote productivity. We thus conclude that dietary supplementation with LGPH not only enhanced CP digestibility but also promoted sufficient production of VFAs which potentially improve weight gain and milk production.

The observed decrease (P< 0.05) in isovalerate and valerate levels in the treatment diets compared to the control diets could be attributed to a lower extent of protein breakdown, since the production of these branched-chain VFAs is related to the fermentation of branched-chain amino acids (Ramos et al., 2009).

NH₃-N in rumen fluids is mainly produced from the proteolytic breakdown of dietary CP and Non-Protein Nitrogen (NPN). It is then being utilized by rumen microbes for the synthesis of amino acid, proteins, and nucleic acids (Hidanah et al., 2016). Its concentration in the rumen fluids was not affected by diet supplementation with LGPH and remained within the normal ranges of 8.5 to 30mg//100ml (McDonald et al., 2002). It is concluded that supplementation with LGPH supplied sufficient fermentable energy for optimal capture of the N produced.

Our findings show that all the values of biochemical parameters fall within the normal physiological range of healthy sheep (Radostits et al., 2007; Kaneko and John Harvey, 2008; Roba et al., 2022). The linear increase in blood TP and BUN in the sheep fed with higher LGPH diets (P15, and P20) could be attributed to corresponding linearly increased dietary CP intake and nutrient digestibility (Yang et al., 1999). Kaneko and John Harvey (2008) showed that serum TP and BUN of healthy sheep range between 6-7.9 g/dL and 10-35mg/dL, respectively. Blood TP levels reflect amino acid synthesis and absorption, while BUN levels accurately indicate short-term dietary impacts on the nitrogen cycle in the rumen, urea synthesis in the liver, recycling, or catabolism (Faryabi et al., 2023). The level of TP in our study were within the normal range, however, BUN was relatively higher in P15, and P20 fed sheep, being 37.4 and 39.3 mg/dL, respectively, probably as a reflection of a high dietary N supply (Luo et al., 2022). The normal levels of serum metabolites enzymes such as creatinine, alanine transferase, aspartate transferase, lactate dehydrogenase, and creatine kinase imply that trace levels of GAs in diets with LGPH didn’t cause adverse toxicological effects on the liver, muscles, and kidney functions in sheep. It is important to note that high GAs in potato vines can be deadly to livestock (Pavlista, 2013). In humans, when the concentration of glycoalkaloids in potato tubers exceeds 200 mg/kg fresh weight, it can cause harmful health effects (Rymuza et al., 2020). As a result, the recommended safe limit for new potato varieties is 100 mg/kg fresh weight (Knuthsen et al., 2009).

In the present study, the low levels of GAs detected in LGPH ranged between 0.4-5.5 µg/g dry weight were probably hydrolysed to solanidine by the rumen microbes (King and McQueen, 1981; El – Shinnawy and Eassawy, 2016). This is consistent with the fact that no observable clinical signs were noticed throughout the trial, suggesting that LGPH is safe and has no toxicological effects on sheep. While our experiment did not primarily focus on growth performance, it is worth noting that we found no significant difference in the daily average weight gain (ADG) of sheep between the LGPH and control diets.