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<front>
<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Agron.</journal-id>
<journal-title>Frontiers in Agronomy</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Agron.</abbrev-journal-title>
<issn pub-type="epub">2673-3218</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="doi">10.3389/fagro.2025.1536935</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Agronomy</subject>
<subj-group>
<subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Assessment of land use and slope influence on soil organic carbon stocks and soil properties in Southern Ethiopia</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author" corresp="yes">
<name>
<surname>Wachu</surname>
<given-names>Cherinet Miju</given-names>
</name>
<xref ref-type="author-notes" rid="fn001">
<sup>*</sup>
</xref>
<uri xlink:href="https://loop.frontiersin.org/people/2863998/overview"/>
<role content-type="https://credit.niso.org/contributor-roles/writing-original-draft/"/>
<role content-type="https://credit.niso.org/contributor-roles/writing-review-editing/"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Ibrahim</surname>
<given-names>Araba Jemal</given-names>
</name>
<uri xlink:href="https://loop.frontiersin.org/people/2987945/overview"/>
<role content-type="https://credit.niso.org/contributor-roles/supervision/"/>
<role content-type="https://credit.niso.org/contributor-roles/visualization/"/>
<role content-type="https://credit.niso.org/contributor-roles/writing-review-editing/"/>
</contrib>
</contrib-group>
<aff id="aff1">
<institution>Department of Soil Resource and Watershed Management, Oda Bultum University</institution>, <addr-line>Chiro</addr-line>,&#xa0;<country>Ethiopia</country>
</aff>
<author-notes>
<fn fn-type="edited-by">
<p>Edited by: Naser A. Anjum, Aligarh Muslim University, India</p>
</fn>
<fn fn-type="edited-by">
<p>Reviewed by: Vikas Sharma, Sant Baba Bhag Singh University, India</p>
<p>Sumit Chakravarty, Uttar Banga Krishi Viswavidyalaya, India</p>
</fn>
<fn fn-type="corresp" id="fn001">
<p>*Correspondence: Cherinet Miju Wachu, <email xlink:href="mailto:cherinetmiju@gmail.com">cherinetmiju@gmail.com</email>
</p>
</fn>
</author-notes>
<pub-date pub-type="epub">
<day>01</day>
<month>07</month>
<year>2025</year>
</pub-date>
<pub-date pub-type="collection">
<year>2025</year>
</pub-date>
<volume>7</volume>
<elocation-id>1536935</elocation-id>
<history>
<date date-type="received">
<day>29</day>
<month>11</month>
<year>2024</year>
</date>
<date date-type="accepted">
<day>02</day>
<month>06</month>
<year>2025</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#xa9; 2025 Wachu and Ibrahim</copyright-statement>
<copyright-year>2025</copyright-year>
<copyright-holder>Wachu and Ibrahim</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/">
<p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p>
</license>
</permissions>
<abstract>
<p>Understanding the impact of land use on soil organic carbon stocks (SOCS) and soil properties is crucial for effective soil management and mitigating climate change. This study aimed to evaluate the effects of land use and slope on SOCS and soil properties in Southern Ethiopia. Soil samples were collected from the upper, middle, and lower slopes, with a total of 54 composite samples representing different land uses. The result revealed that total nitrogen, potassium, and organic carbon in land use and slope position were significantly affected. The highest soil organic carbon stocks, total nitrogen, and organic carbon values were observed in the enset-based land-use system, which also recorded. In lower slope showed the lowest levels of organic carbon (3.27%), total nitrogen (0.28%), available phosphorus (3.58 ppm), and SOCS (73.38 mg ha<sup>&#x2212;1</sup>). The results indicated that different land-use systems influence the soil&#x2019;s physicochemical properties and the variation of slope varies in forestry and enset land use system results of the soil properties studied. Based on these studies forestry and enset land system tremendous potential raise soil fertility capacity and diminish loss of soil nutrient and more conservation minded. Therefore, we concluded that using different plant residual materials and mixed farming methods, such as agroforestry, promotes the sustainable maintenance of soil physicochemical properties for both the present and the future. This underscores the need for integrated land management to boost carbon storage, enhance soil fertility, and combat climate change.</p>
</abstract>
<kwd-group>
<kwd>land use impact</kwd>
<kwd>soil organic carbon stocks</kwd>
<kwd>slop variation</kwd>
<kwd>soil fertility conservation</kwd>
<kwd>sustainable land management</kwd>
</kwd-group>
<counts>
<fig-count count="1"/>
<table-count count="3"/>
<equation-count count="2"/>
<ref-count count="67"/>
<page-count count="10"/>
<word-count count="5467"/>
</counts>
<custom-meta-wrap>
<custom-meta>
<meta-name>section-in-acceptance</meta-name>
<meta-value>Plant-Soil Interactions</meta-value>
</custom-meta>
</custom-meta-wrap>
</article-meta>
</front>
<body>
<sec id="s1" sec-type="intro">
<label>1</label>
<title>Introduction</title>
<p>Soil is related to land use, and the interaction between physical and chemical properties makes it a functionally complete resource. This resource is utilized for various purposes, such as agricultural, forestry, and urban development. Conversely, different land-use practices can affect soil health, diminishing productivity, the capacity of erosion resistance, and overall sustainability. Increasing human activity, particularly in land use, is a major cause of variations in soil properties and soil organic carbon stocks (SOCS) on a global scale (<xref ref-type="bibr" rid="B4">Agidew and Singh, 2018</xref>). In addition, anthropogenic activities and land-use changes may lead to reduced soil structure stability, loss of organic matter (OM), and a decline in nutrient stocks (<xref ref-type="bibr" rid="B47">Mohammad et&#xa0;al., 2005</xref>). Currently, approximately 33% of global soils are moderately to highly degraded. Of these degraded soils, 40% are located in Africa, primarily in regions affected by poverty and food insecurity (<xref ref-type="bibr" rid="B37">Karlen and Rice, 2018</xref>; <xref ref-type="bibr" rid="B55">Shaikh, 2018</xref>). According to global assessments, the proportion of severely degraded land grew from 15% in 1991 to 25% in 2011. In 2008, degradation affected over 20% of intensively used agricultural land, 30% of virgin forests, and 25% of grazing land (<xref ref-type="bibr" rid="B62">UNCCD, 2013</xref>; <xref ref-type="bibr" rid="B40">Kimura, 2018</xref>). Various land-use practices and their associated management techniques have altered soil physical and chemical properties.</p>
<p>The type of land-use system is a significant factor influencing soil organic carbon (SOC) levels, as it affects the quantity and quality of litter inputs, the decomposition rate, and the stabilization processes of OM in soil (<xref ref-type="bibr" rid="B21">C&#xf3;rdova et&#xa0;al., 2018</xref>). SOC losses are influenced by variations in land-use practices and management techniques (<xref ref-type="bibr" rid="B5">Alemayehu and Sheleme, 2013</xref>). Both biotic and abiotic factors determine the spatial and temporal variability of SOC storage (<xref ref-type="bibr" rid="B64">Weissert et&#xa0;al., 2016</xref>). Globally, hotspots like wetlands and peatlands&#x2014;mainly located in arctic and tropical regions&#x2014;contain the largest SOC reserves (<xref ref-type="bibr" rid="B31">Gougoulias et&#xa0;al., 2014</xref>; <xref ref-type="bibr" rid="B41">K&#xf6;chy et&#xa0;al., 2015</xref>). Additional high SOC concentrations are found in deep soil horizons (&gt;1 m). Global estimates suggest that SOCS in the topsoil layer are approximately 1,500 PgC (<xref ref-type="bibr" rid="B27">FAO, 2015</xref>).</p>
<p>Land-use changes significantly impact net primary production, species composition, stand age, root distribution, and litter quantity. Low SOC levels and other poor soil properties are key drivers of soil erosion. Substantial SOC losses from surface and subsurface soil layers, caused by accelerated soil erosion, can disrupt the global carbon cycle, as even slight changes in SOC pools can significantly affect carbon dynamics (<xref ref-type="bibr" rid="B66">Zhang et&#xa0;al., 2015</xref>). These changes in SOC storage can either exacerbate or mitigate climate change (<xref ref-type="bibr" rid="B30">Girma et&#xa0;al., 2020</xref>).</p>
<p>Variations in land use&#x2014;particularly in natural versus managed systems, such as agroforestry, cultivated lands, grasslands, and grazing lands&#x2014;alter soil properties and carbon balance (<xref ref-type="bibr" rid="B12">Berry, 2011</xref>). These changes have both positive and negative impacts on agricultural productivity and carbon stocks. Research has shown that natural ecosystems and managed agroecosystems exhibit significant differences in soil property status (<xref ref-type="bibr" rid="B49">Mulugeta et&#xa0;al., 2005</xref>; <xref ref-type="bibr" rid="B50">Nega and Heluf, 2013</xref>). Factors such as land-use type, vegetation cover, cultivation intensity, and fertilizer sources are key drivers of soil physical and chemical property variations (<xref ref-type="bibr" rid="B2">Abera and Wolde-Meskel, 2013</xref>).</p>
<p>Recent studies in Southern Ethiopia highlight that variations in soil properties, SOCS, and soil fertility are closely related to land-use management practices (<xref ref-type="bibr" rid="B8">Assefa et&#xa0;al., 2020</xref>). For instance, higher concentrations of SOC, total nitrogen (TN), cation exchange capacity (CEC), available phosphorus (Av. P), and exchangeable potassium (K) have been recorded in enset-based farming systems in Southern Ethiopia (<xref ref-type="bibr" rid="B58">Shrestha and Kafle, 2020</xref>; <xref ref-type="bibr" rid="B65">Wolka et&#xa0;al., 2021</xref>).</p>
<p>This study aims to evaluate the effects of land use and topography on soil properties. The landscape of the study area manifests complex land use and soil conservation practices; in this case, it may be the factor that affects soil properties and reduces productivity of soil. Understanding these influences of land use and slope is essential for sustainable land management and climate change mitigation.</p>
</sec>
<sec id="s2" sec-type="materials|methods">
<label>2</label>
<title>Materials and methods</title>
<sec id="s2_1">
<label>2.1</label>
<title>Description of the study area</title>
<p>This study was conducted in the Gedeo zone in the Kochore district of the southern part of Ethiopia (<xref ref-type="fig" rid="f1">
<bold>Figure&#xa0;1</bold>
</xref>). The study area is located 410 km away from Addis Ababa toward the south and approximately 120 km south of Hawassa, the capital city of the Sidamo region. The absolute location of the study area lies in 6&#x2da; 8&#x2032; 0&#x2033;&#x2013;5&#x2da; 55&#x2032; 5&#x2033; N latitude and 38&#x2da; 4&#x2032; 0&#x2033;&#x2013;38&#x2da; 20&#x2032; 0&#x2033; E longitude, and the area spans an altitude range of 1,500 to 2,200 m above sea level. The study area categorizes wone-dege based on the local climate. The study area with a slope ranging from gentle and medium. The area experiences a bimodal rainfall pattern, with the major rain season (kiremt or mehar) beginning in July up to November and the short rainy season from February to April (&#x201c;Belg&#x201d;) with the area characterized by a humid climate with an average yearly rainfall of 1,100 to 1,800 mm (<xref ref-type="bibr" rid="B33">Haile et&#xa0;al., 2024</xref>). The average monthly temperature of the study site ranges from 17&#xb0;C to 21&#xb0;C (<xref ref-type="bibr" rid="B33">Haile et&#xa0;al., 2024</xref>).</p>
<fig id="f1" position="float">
<label>Figure&#xa0;1</label>
<caption>
<p>Map of study area (ArcGIS).</p>
</caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fagro-07-1536935-g001.tif">
<alt-text content-type="machine-generated">Map illustrating the study area in South Ethiopia, highlighting Gedeo Zone. The map includes separate panels for Study Area Map, South Ethiopia, Gedeo Zone, and Ethiopia Regions. A legend indicates regions with colors: South Region in yellow, Kochore Woreda in light yellow, Gedeo Zone in green, and Ethio Regions in light blue. A north arrow is at the top right.</alt-text>
</graphic>
</fig>
<p>The major soil types of the study area are classified. The soil types dominant in the area include <italic>Eutric Nitosol</italic> (58%), <italic>Eutric Fluvisols</italic> (30%), and <italic>Dystric Nitosol</italic> (10%) (<xref ref-type="bibr" rid="B28">FAO/IAEA, 2018</xref>). The total population in the study area is 188,323, of whom 93,050 were male and 95,273 were female (<xref ref-type="bibr" rid="B25">StatsEthiopia.gov.et/wp-content/uploads/2023/08/Population-of-Zones-and-WeredasProjected-as-of-July-2023.pdf</xref>). Forestry, cultivated, and enset are the predominant land use in the study area (<xref ref-type="bibr" rid="B65">Wolka et&#xa0;al., 2021</xref>). A variety of tree species&#x2014;<italic>Croton macrostachyus</italic>, Wanza (<italic>Cordia africana</italic>), Ficus sycomours, birbira (<italic>Millettia ferruginea</italic>), and <italic>Erythrina abyssinica</italic>&#x2014;are found in the forest. The major crops grown are maize (<italic>Zea mays</italic>), coffee (<italic>Coffee arabica</italic>), enset, wheat, cabbage (<italic>Brassica oleracca</italic>), teff, and tubers (root crop), all grown in cultivated land. People depend on agricultural production and processing for the domestic market, especially when it comes to coffee and enset. Eighty-five percent (21,890 ha) of the land is utilized for growing both annual and perennial crops.</p>
</sec>
<sec id="s2_2">
<label>2.2</label>
<title>Soil sampling and preparation</title>
<p>The land-use types were chosen in an area adjacent to the study area to minimize variations of error. According to <xref ref-type="bibr" rid="B26">FAO (2006)</xref>, there are three slope classifications in the study site: lower (5%&#x2013;10%), middle (10%&#x2013;15%), and upper slope class (&gt;15%), and three land-use types (enset, forest, and cultivated land) were selected. Enset crops dominate the land cover because farmers plant these crops to sustain food security. The government encourages the planting of enset crops. Each farmer is required to plant enset crop on their land to ensure food sustainability, as mandated by the regional and zonal governments in south Ethiopia. Hence, total composite samples are 54 disturbed soil sample collected from three slopes. However, the other undisturbed 54 soil samples were taken to determine bulk density. The soil samples were meticulously separated from undesired material such as plant and animal remains, gravel, and soil. In addition, soil samples taken from four corners and one center of square plots were used to collect both disturbed and undisturbed soil, at depths of 0&#x2013;20 cm, and while a sample of disturbed soil was collected using an auger to analyze the physicochemical properties of the soil, a sample of undisturbed soil were gathered using a core sampler for determine soil bulk density (<xref ref-type="disp-formula" rid="eq1">Equation 1</xref>).</p>
<disp-formula id="eq1">
<label>(1)</label>
<mml:math display="block" id="M1">
<mml:mrow>
<mml:mi>T</mml:mi>
<mml:mi>P</mml:mi>
<mml:mo stretchy="false">(</mml:mo>
<mml:mo>%</mml:mo>
<mml:mo stretchy="false">)</mml:mo>
<mml:mo>=</mml:mo>
<mml:mn>1</mml:mn>
<mml:mo>&#x2212;</mml:mo>
<mml:mfrac>
<mml:mrow>
<mml:mi>b</mml:mi>
<mml:mi>u</mml:mi>
<mml:mi>l</mml:mi>
<mml:mi>k</mml:mi>
<mml:mo>&#xa0;</mml:mo>
<mml:mi>d</mml:mi>
<mml:mi>e</mml:mi>
<mml:mi>n</mml:mi>
<mml:mi>s</mml:mi>
<mml:mi>i</mml:mi>
<mml:mi>t</mml:mi>
<mml:mi>y</mml:mi>
</mml:mrow>
<mml:mrow>
<mml:mi>p</mml:mi>
<mml:mi>a</mml:mi>
<mml:mi>r</mml:mi>
<mml:mi>t</mml:mi>
<mml:mi>i</mml:mi>
<mml:mi>c</mml:mi>
<mml:mi>l</mml:mi>
<mml:mi>e</mml:mi>
<mml:mo>&#xa0;</mml:mo>
<mml:mi>d</mml:mi>
<mml:mi>e</mml:mi>
<mml:mi>n</mml:mi>
<mml:mi>s</mml:mi>
<mml:mi>i</mml:mi>
<mml:mi>t</mml:mi>
<mml:mi>y</mml:mi>
</mml:mrow>
</mml:mfrac>
<mml:mo>&#xd7;</mml:mo>
<mml:mn>100</mml:mn>
</mml:mrow>
</mml:math>
</disp-formula>
</sec>
<sec id="s2_3">
<label>2.3</label>
<title>Soil laboratory analysis</title>
<p>Soil samples were air-dried at room temperature, then sieved through a 2-mm sieve and prepared for the determination of soil physical and chemical properties. Soil samples were also sieved through a 0.5-mm sieve for the determination of SOC. The analysis was conducted using the oxidation method to determine the organic carbon (OC) value (<xref ref-type="bibr" rid="B13">Blake, 1965</xref>). The exchangeable basic cations (K<sup>+</sup>, Ca<sup>2+</sup>, Mg<sup>2+</sup>, and Na<sup>+</sup>), have been extracted with 1 M ammonium acetate at pH 7.0. The CEC of the soil was determined from ammonium acetate saturate sample. The excess ammonium acetate is removed by washing with ethanol. Finally, the exchangeable K<sup>+</sup> and Na<sup>+</sup> determined with flame photometer Mg<sup>2+</sup> and Ca<sup>2+</sup> have been determined by atomic absorption spectra. The hydrometer method was used for the determination of soil particle size distribution (<xref ref-type="bibr" rid="B16">Bouyoucos, 1962</xref>). The soil pH was measured using a glass combination pH meter in the supernatant solution of 1:2.5 soil-to-water solution ratio (<xref ref-type="bibr" rid="B63">Van Reeuwijk, 1992</xref>). TN was conducted using Kjeldah (<xref ref-type="bibr" rid="B13">Blake, 1965</xref>), and available phosphorus (P) (<xref ref-type="bibr" rid="B51">Olsen et&#xa0;al., 1954</xref>), potassium (K) (<xref ref-type="bibr" rid="B63">Van Reeuwijk, 1992</xref>), and bulk density (B d) (<xref ref-type="bibr" rid="B13">Blake, 1965</xref>) were analyzed.</p>
</sec>
<sec id="s2_4">
<label>2.4</label>
<title>Calculation of soil organic carbon stock</title>
<p>Soil organic carbon content obtained from laboratory analysis was used to calculate carbon stock per unit area of land. Soil organic carbon stock was determined for each soil depth under different land use types according to <xref ref-type="bibr" rid="B67">Zhang et&#xa0;al., 2008</xref> (<xref ref-type="disp-formula" rid="eq2">Equation 2</xref>) </p>
<disp-formula id="eq2">
<label>(2)</label>
<mml:math display="block" id="M2">
<mml:mrow>
<mml:mi>S</mml:mi>
<mml:mi>O</mml:mi>
<mml:mi>C</mml:mi>
<mml:mi>S</mml:mi>
<mml:mo>=</mml:mo>
<mml:mi>D</mml:mi>
<mml:mi>i</mml:mi>
<mml:mo>&#xa0;</mml:mo>
<mml:mo>&#xd7;</mml:mo>
<mml:mi>B</mml:mi>
<mml:mi>D</mml:mi>
<mml:mo>&#xd7;</mml:mo>
<mml:mo>%</mml:mo>
<mml:mi>S</mml:mi>
<mml:mi>O</mml:mi>
<mml:mi>C</mml:mi>
<mml:mo>&#xa0;</mml:mo>
<mml:mo>&#xd7;</mml:mo>
<mml:mrow>
<mml:mo stretchy="false">(</mml:mo>
<mml:mrow>
<mml:mn>1</mml:mn>
<mml:mo>&#x2212;</mml:mo>
<mml:mi>C</mml:mi>
<mml:mi>F</mml:mi>
</mml:mrow>
<mml:mo stretchy="false">)</mml:mo>
</mml:mrow>
<mml:mo>&#xd7;</mml:mo>
<mml:mn>100</mml:mn>
</mml:mrow>
</mml:math>
</disp-formula>
<p>where</p>
<p>SOCS = soil organic carbon stock of the layer (t/ha),</p>
<p>Di = the depth of the soil sample thickness of the sampled soil layer (cm),</p>
<p>BD = soil bulk density of the soil layer (g cm<sup>&#x2212;3</sup>),</p>
<p>% SOCi = soil organic carbon content in the soil layer (%),</p>
<p>C Fi =coarse fragment content of the soil layer&gt; 2 mm (%), and</p>
<p>100 is the conversion factor from g/cm<sup>2</sup> to t/ha.</p>
<p>A coarse fraction was determined during sample preparation after the repeated crushing of clods by hand, mechanical grinding, drying, and sieving until the sample was passed through a 2-mm sieve. However, most of the fraction of fragments were below 2 mm, which are fine particles and excluded from the calculation.</p>
</sec>
<sec id="s2_5">
<label>2.5</label>
<title>Statistical analysis</title>
<p>Statistical analysis of the soil data was performed on replicated sample using General Linear Model( GLM) procedures to determine significant differences among parameters related to slope and land use. Mean separation was done by least significance difference (LSD) for those attributes that are significantly different. All statistical analyses were conducted using statistical software (SAS, 2004) version 9.4 following the two-way ANOVA procedure.</p>
</sec>
</sec>
<sec id="s3" sec-type="results">
<label>3</label>
<title>Results and discussion</title>
<sec id="s3_1">
<label>3.1</label>
<title>Effects of land use and slope position on soil physical properties</title>
<sec id="s3_1_1">
<label>3.1.1</label>
<title>Soil particle size</title>
<p>Clay particle size varied significantly (<italic>p</italic> &lt; 0.001) and was affected by land use (<xref ref-type="table" rid="T1">
<bold>Table&#xa0;1</bold>
</xref>). Relatively, the highest clay content, 55.2%, was found in cultivated land and the lowest, 50%, was observed in forestry land. Similarly, clay particle size was significantly influenced by slope position. The average sand fraction was the lowest (21.39%) in the upper slope position and the highest (25.33%) in the lower slope position.</p>
<table-wrap id="T1" position="float">
<label>Table&#xa0;1</label>
<caption>
<p>Effects of land use and slope on soil physical properties.</p>
</caption>
<table frame="hsides">
<thead>
<tr>
<th valign="top" align="left">Land uses</th>
<th valign="top" align="left">Clay</th>
<th valign="top" align="left">Silt</th>
<th valign="top" align="left">Sand</th>
<th valign="top" align="left">Bulk density (g cm<sup>&#x2212;1</sup>)</th>
<th valign="top" align="left">Total porosity (%)</th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">Forestry</td>
<td valign="top" align="left">50<sup>b</sup>
</td>
<td valign="top" align="left">20.61<sup>b</sup>
</td>
<td valign="top" align="left">28.38<sup>a</sup>
</td>
<td valign="top" align="left">1.00<sup>b</sup>
</td>
<td valign="top" align="left">62.22<sup>a</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">Enset</td>
<td valign="top" align="left">50.5<sup>b</sup>
</td>
<td valign="top" align="left">30.44<sup>a</sup>
</td>
<td valign="top" align="left">21.5<sup>b</sup>
</td>
<td valign="top" align="left">1.03<sup>b</sup>
</td>
<td valign="top" align="left">60.96<sup>a</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">Cultivated</td>
<td valign="top" align="left">55.2a</td>
<td valign="top" align="left">23.28<sup>b</sup>
</td>
<td valign="top" align="left">19.06<sup>b</sup>
</td>
<td valign="top" align="left">1.13<sup>a</sup>
</td>
<td valign="top" align="left">57.48<sup>b</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">CV (%)</td>
<td valign="top" align="left">9.01</td>
<td valign="top" align="left">16.4</td>
<td valign="top" align="left">13.4</td>
<td valign="top" align="left">7.34</td>
<td valign="top" align="left">4.85</td>
</tr>
<tr>
<td valign="top" align="left">LSD</td>
<td valign="top" align="left">3.16</td>
<td valign="top" align="left">2.78</td>
<td valign="top" align="left">2.51</td>
<td valign="top" align="left">0.06</td>
<td valign="top" align="left">2.38</td>
</tr>
<tr>
<td valign="top" align="left">
<italic>p</italic>-value</td>
<td valign="top" align="left">**</td>
<td valign="top" align="left">***</td>
<td valign="top" align="left">***</td>
<td valign="top" align="left">**</td>
<td valign="top" align="left">**</td>
</tr>
<tr>
<th valign="top" colspan="6" align="center">Slope</th>
</tr>
<tr>
<td valign="top" align="left">Lower 5&#x2013;10</td>
<td valign="top" align="left">60.89<sup>a</sup>
</td>
<td valign="top" align="left">17.72<sup>c</sup>
</td>
<td valign="top" align="left">21.39<sup>b</sup>
</td>
<td valign="top" align="left">1.15<sup>a</sup>
</td>
<td valign="top" align="left">56.58<sup>c</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">Middle 10&#x2013;15</td>
<td valign="top" align="left">52.44<sup>b</sup>
</td>
<td valign="top" align="left">25.33<sup>b</sup>
</td>
<td valign="top" align="left">22.22<sup>b</sup>
</td>
<td valign="top" align="left">1.07<sup>b</sup>
</td>
<td valign="top" align="left">59.5<sup>b</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">Upper &gt;15</td>
<td valign="top" align="left">42.39<sup>c</sup>
</td>
<td valign="top" align="left">32.28<sup>a</sup>
</td>
<td valign="top" align="left">25.33<sup>a</sup>
</td>
<td valign="top" align="left">0.94<sup>c</sup>
</td>
<td valign="top" align="left">64.59<sup>a</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">LSD</td>
<td valign="top" align="left">3.16</td>
<td valign="top" align="left">2.78</td>
<td valign="top" align="left">2.51</td>
<td valign="top" align="left">0.06</td>
<td valign="top" align="left">2.38</td>
</tr>
<tr>
<td valign="top" align="left">
<italic>p</italic>-value</td>
<td valign="top" align="left">***</td>
<td valign="top" align="left">***</td>
<td valign="top" align="left">**</td>
<td valign="top" align="left">***</td>
<td valign="top" align="left">***</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn>
<p>Interaction effect means within a row (land use with soil depth of a given parameter) followed by the same letter(s) are not significantly different from each other at <italic>p</italic> &#x2264; 0.05. LSD, Least significant difference.</p>
</fn>
<fn>
<p>** medium significant difference. ***- highly significant difference.</p>
</fn>
</table-wrap-foot>
</table-wrap>
<p>This may be attributed to continuous plowing, soil disturbance, and the removal of topsoil in cultivated areas, which accelerate weathering processes and transform silt and sand into clay. Additionally, the relatively high clay content in cultivated land may result from tillage and deforestation, as these practices increase clay formation through accelerated weathering. Years of continuous tillage can enhance the clay content in the plow layer by altering the soil&#x2019;s moisture, aeration, and temperature regimes, which, in turn, influence the rate of chemical reactions. These results align with previous studies (<xref ref-type="bibr" rid="B20">Chimdi and Gurmessa, 2023</xref>; <xref ref-type="bibr" rid="B60">Suh and Tsheko, 2024</xref>), which found that cultivated land has a higher clay content compared to other land uses.</p>
<p>We observed the highest clay content at lower slope positions. This increased clay content can significantly influence soil properties, affecting nutrient availability, drainage, and overall soil fertility. As a result, the lower slope areas often support different vegetation and agricultural practices compared to the upper slopes (<xref ref-type="bibr" rid="B33">Haile et&#xa0;al., 2024</xref>). However, it is consistent with other studies (<xref ref-type="bibr" rid="B14">Bojago et&#xa0;al., 2023</xref>; <xref ref-type="bibr" rid="B9">Atinafu et&#xa0;al., 2024</xref>), which found that the highest clay content was recorded at the lower slope of the gradient. It may be because the lower slope is composed of finer-textured parent material, or if there is prolonged weathering in that area, clay content may naturally be higher.</p>
<p>Forestry (20.61%) and enset (30.44%) systems show contrasting silt contents, possibly due to differences in vegetation cover and soil disturbance. The higher silt in enset could be attributed to OM retention and reduced erosion compared to cultivated systems (<xref ref-type="bibr" rid="B22">Demeku et&#xa0;al., 2022</xref>). Silt distribution is strongly influenced by slope-induced erosion higher on steep slopes and land management reduced in cultivated soils.</p>
<p>The sand fraction size was also significantly (<italic>p</italic> &lt; 0.05) affected by land use (<xref ref-type="table" rid="T2">
<bold>Table&#xa0;2</bold>
</xref>). The highest sand content at the lower slope position may be due to the lack of movement in this area, as well as the absence of sand particle translocation within the lower slope soil.</p>
<table-wrap id="T2" position="float">
<label>Table&#xa0;2</label>
<caption>
<p>Effects of land use and slope position on soil chemical properties.</p>
</caption>
<table frame="hsides">
<thead>
<tr>
<th valign="top" align="left">Land uses</th>
<th valign="top" align="left">pH</th>
<th valign="top" align="left">SOC%</th>
<th valign="top" align="left">TN%</th>
<th valign="top" align="left">Av. <italic>P</italic> (ppm)</th>
<th valign="top" align="left">SOCS (mg ha<sup>&#x2212;1</sup>)</th>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left">Forestry</td>
<td valign="top" align="left">6.5<sup>a</sup>
</td>
<td valign="top" align="left">4.83<sup>a</sup>
</td>
<td valign="top" align="left">0.21<sup>b</sup>
</td>
<td valign="top" align="left">3.6<sup>b</sup>
</td>
<td valign="top" align="left">95.52<sup>a</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">Enset</td>
<td valign="top" align="left">6.3<sup>a</sup>
</td>
<td valign="top" align="left">4.60<sup>a</sup>
</td>
<td valign="top" align="left">0.39<sup>a</sup>
</td>
<td valign="top" align="left">4.64<sup>a</sup>
</td>
<td valign="top" align="left">94.73<sup>a</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">Cultivated</td>
<td valign="top" align="left">5.8<sup>b</sup>
</td>
<td valign="top" align="left">2.55<sup>b</sup>
</td>
<td valign="top" align="left">0.41<sup>a</sup>
</td>
<td valign="top" align="left">2.9<sup>c</sup>
</td>
<td valign="top" align="left">54.68<sup>b</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">CV (%)</td>
<td valign="top" align="left">4.74</td>
<td valign="top" align="left">14.69</td>
<td valign="top" align="left">14.69</td>
<td valign="top" align="left">16.3</td>
<td valign="top" align="left">14.47</td>
</tr>
<tr>
<td valign="top" align="left">LSD</td>
<td valign="top" align="left">0.24</td>
<td valign="top" align="left">0.48</td>
<td valign="top" align="left">0.04</td>
<td valign="top" align="left">0.5</td>
<td valign="top" align="left">9.63</td>
</tr>
<tr>
<td valign="top" align="left">
<italic>p</italic>-value</td>
<td valign="top" align="left">**</td>
<td valign="top" align="left">**</td>
<td valign="top" align="left">**</td>
<td valign="top" align="left">***</td>
<td valign="top" align="left">**</td>
</tr>
<tr>
<th valign="top" colspan="6" align="center">Slope position</th>
</tr>
<tr>
<td valign="top" align="left">Lower 5&#x2013;10</td>
<td valign="top" align="left">6.2<sup>a</sup>
</td>
<td valign="top" align="left">4.67<sup>a</sup>
</td>
<td valign="top" align="left">0.40<sup>a</sup>
</td>
<td valign="top" align="left">4.05<sup>a</sup>
</td>
<td valign="top" align="left">87.03<sup>b</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">Middle 10&#x2013;15</td>
<td valign="top" align="left">5.9<sup>a</sup>
</td>
<td valign="top" align="left">4.05<sup>b</sup>
</td>
<td valign="top" align="left">0.34<sup>b</sup>
</td>
<td valign="top" align="left">3.64<sup>ab</sup>
</td>
<td valign="top" align="left">84.52<sup>a</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">Upper &gt;15</td>
<td valign="top" align="left">5.4<sup>a</sup>
</td>
<td valign="top" align="left">3.27<sup>c</sup>
</td>
<td valign="top" align="left">0.28<sup>c</sup>
</td>
<td valign="top" align="left">3.53<sup>b</sup>
</td>
<td valign="top" align="left">73.38<sup>b</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">LSD</td>
<td valign="top" align="left">2.24</td>
<td valign="top" align="left">0.48</td>
<td valign="top" align="left">0.04</td>
<td valign="top" align="left">0.5</td>
<td valign="top" align="left">9.63</td>
</tr>
<tr>
<td valign="top" align="left">
<italic>p</italic>-value</td>
<td valign="top" align="left">Ns</td>
<td valign="top" align="left">***</td>
<td valign="top" align="left">***</td>
<td valign="top" align="left">*</td>
<td valign="top" align="left">*</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn>
<p>
<italic>*</italic>Interaction effect means within a row (land use with soil depth of a given parameter) followed by the same letter(s) are not significantly different from each other at <italic>p</italic> &#x2264; 0.05. LSD, Least significant difference.</p>
</fn>
<fn>
<p>*- significant difference. ** medium significant difference. ***- highly significant difference.</p>
</fn>
</table-wrap-foot>
</table-wrap>
<p>Additionally, the lack of clear translocation and accumulation of finer fractions at lower parts of slopes has been attributed to the length of slopes in young glacial landscapes. In such terrains, soil material is translocated due to anthropogenic denudation, with more pronounced changes observed at the lower slope and in depressions (<xref ref-type="bibr" rid="B55">Shaikh, 2018</xref>).</p>
</sec>
<sec id="s3_1_2">
<label>3.1.2</label>
<title>Bulk density</title>
<p>The highest BD was recorded in cultivated land and the lowest was recorded in forestry land. BD of the soils varied significantly (<italic>p</italic> &lt; 0.05) across different land uses (<xref ref-type="table" rid="T1">
<bold>Table&#xa0;1</bold>
</xref>). Several studies have observed that cultivated lands often exhibit higher bulk density due to intensive agricultural practices. For instance, research indicates that cultivated land increases soil bulk density as a result of animal trampling, which leads to soil compaction (<xref ref-type="bibr" rid="B47">Mohammad et&#xa0;al., 2005</xref>; <xref ref-type="bibr" rid="B59">Spohn et&#xa0;al., 2023</xref>; <xref ref-type="bibr" rid="B1">Abera et&#xa0;al., 2024</xref>; <xref ref-type="bibr" rid="B9">Atinafu et&#xa0;al., 2024</xref>). Additionally, the removal of crop residues and continuous cultivation have been associated with increased soil compaction, contributing to higher BD.</p>
<p>The lowest BD (1 g/cm&#xb3;) was observed in forestry land (<xref ref-type="table" rid="T1">
<bold>Table&#xa0;1</bold>
</xref>), which could be attributed to farming methods that increase OM and subsequently lower BD. This finding aligns with the results of Rigal et&#xa0;al (<xref ref-type="bibr" rid="B52">Rigal et&#xa0;al., 2019</xref>), which highlighted that continuous animal and household waste additions, along with increased litter deposition from plant cover, enhance soil OM storage and reduce BD.</p>
<p>This study indicates that BD varies with slope position, often showing lower values at upper slope positions and higher values at lower slope positions. Factors including soil compaction, OM content, and erosion processes all have an impact on this pattern. For instance, a study observed that the upper slope position, which had a higher clay content, exhibited a lower bulk density compared to lower slope positions (<xref ref-type="bibr" rid="B35">Ivan et&#xa0;al., 2022</xref>; <xref ref-type="bibr" rid="B34">Hailu et&#xa0;al., 2024</xref>).</p>
<p>However, it is important to note that other studies have reported different findings (<xref ref-type="bibr" rid="B42">Kucuk, 2019</xref>; <xref ref-type="bibr" rid="B11">Bayle et&#xa0;al., 2023</xref>). Research has shown that lower slope positions recorded the highest values for clay content and other soil properties, while BD was higher in the upper slope position.</p>
</sec>
<sec id="s3_1_3">
<label>3.1.3</label>
<title>Total porosity</title>
<p>The total porosity (TP) of the soil was highest at 57.48% in cultivated land and at 62.22% in forestry land (<xref ref-type="table" rid="T1">
<bold>Table&#xa0;1</bold>
</xref>). The higher TP in forestry land soil may be attributed to crop residues, earthworms, and OM, which improve soil porosity. This finding is consistent with the work of <xref ref-type="bibr" rid="B36">Jemal and Tesfaye (2020)</xref>, which observed that forest land utilization exhibited higher TP compared to other land uses due to high OC content and the role of earthworms in decomposing plant leaves. In addition, studies (<xref ref-type="bibr" rid="B3">Abukari et&#xa0;al., 2021</xref>; <xref ref-type="bibr" rid="B9">Atinafu et&#xa0;al., 2024</xref>) show that vegetation leaf decomposition enhances microbial activity, which reduces bulk density, increases soil organic matter (SOM), and improves soil porosity. TP varied significantly (<italic>p</italic> &lt; 0.001) with slope position. Among slope positions, the highest TP (64.59%) was recorded at the upper slope, while the lowest TP (56.58%) was observed at the lower slope (<xref ref-type="table" rid="T1">
<bold>Table&#xa0;1</bold>
</xref>). Soil compaction, erosion, and the distribution of OM are some of the factors contributing to differences in total soil porosity across slope positions (<xref ref-type="bibr" rid="B49">Mulugeta et&#xa0;al., 2005</xref>). Lower slopes absorb eroded materials from higher places, including finer particles that diminish macropore space and increase bulk density, leading to lower porosity (<xref ref-type="bibr" rid="B48">Morgan and Royston, 2006</xref>). Upper slope positions have less foot traffic and machinery impact than lower slope sites, decreasing soil compaction and retaining higher porosity (<xref ref-type="bibr" rid="B61">Tetengi et&#xa0;al., 2022</xref>).</p>
</sec>
</sec>
<sec id="s3_2">
<label>3.2</label>
<title>Effects of land use and slope position on soil chemical properties and soil organic carbon stocks</title>
<sec id="s3_2_1">
<label>3.2.1</label>
<title>Soil reactions</title>
<p>The highest pH value was recorded in forestry land, while the lowest pH value was observed in cultivated land (<xref ref-type="table" rid="T2">
<bold>Table&#xa0;2</bold>
</xref>). Soil pH (H<sub>2</sub>O) varied significantly (<italic>p</italic> &lt; 0.001) across land uses. In this study, cultivated land is subject to various land-use practices. Farmers apply different inorganic fertilizers, which may contribute to the lower pH. In addition, lower pH in cultivated land may be due to the fact that the application of nitrogen-based fertilizers (e.g., urea and ammonium sulfate) contributes to soil acidification by increasing hydrogen ion concentration (<xref ref-type="bibr" rid="B32">Guo et&#xa0;al., 2010</xref>; <xref ref-type="bibr" rid="B36">Jemal and Tesfaye, 2020</xref>; <xref ref-type="bibr" rid="B33">Haile et&#xa0;al., 2024</xref>).</p>
<p>On the other hand, soil pH beneath shade trees such as <italic>Croton macrostachyus</italic> (commonly known as &#x201c;Wanza&#x201d;), <italic>Cordia africana</italic> (&#x201c;Grare&#x201d; in local name), <italic>Ficus vasta</italic> (&#x201c;Warka&#x201d;), and <italic>Millettia ferruginea</italic> (&#x201c;Birbira&#x201d;) significantly differed from the pH in open-field areas (<xref ref-type="bibr" rid="B7">Aschalew and Zebene, 2018</xref>). Various studies support this phenomenon, showing that the soil under tree-shaded areas has a noticeably higher pH compared to open-field soil (<xref ref-type="bibr" rid="B15">Bore and Bedad, 2016</xref>; <xref ref-type="bibr" rid="B36">Jemal and Tesfaye, 2020</xref>). Forest land has a higher pH due to its high OM content, limited soil disturbance, and cation buildup, whereas cultivated land experience acidification due to intensive farming, fertilizer application, and cation leaching (<xref ref-type="bibr" rid="B24">Dessalegn et&#xa0;al., 2014</xref>).</p>
</sec>
<sec id="s3_2_2">
<label>3.2.2</label>
<title>Organic carbon</title>
<p>SOC was significantly affected (<italic>p</italic> &lt; 0.001) by land-use types, including cultivated, forestry, and enset land. The highest SOC was recorded in forestry land and the lowest was recorded in cultivated land, likely due to tree canopies, vegetation cover, and cro p residues, which contribute to the accumulation of OC in forested areas. The highest SOC in forestry land (4.83%), despite its higher pH (6.5) compared to cultivated land (pH 5.8, SOC 2.55%), can be attributed to greater OM inputs (leaf litter and root exudates) and minimal soil disturbance, which enhance carbon sequestration while maintaining near-neutral pH due to buffering by base cations (Ca&#xb2;<sup>+</sup> and Mg&#xb2;<sup>+</sup>) from decomposing biomass (<xref ref-type="bibr" rid="B18">Cherinet et&#xa0;al., 2025</xref>). Similar findings from various regions in Ethiopia have demonstrated higher SOC levels under the canopies of coffee and shade trees such as Wanza (<italic>Cordia africana</italic>), Warka (<italic>Ficus vasta</italic>), and Birbira (<italic>Millettia ferruginea</italic>) compared to areas outside their canopies (<xref ref-type="bibr" rid="B7">Aschalew and Zebene, 2018</xref>; <xref ref-type="bibr" rid="B65">Wolka et&#xa0;al., 2021</xref>).</p>
<p>The highest SOC value of 4.67% was recorded in the lower slope, while the lowest value of 3.27% was observed in the upper slope (<xref ref-type="table" rid="T2">
<bold>Table&#xa0;2</bold>
</xref>). Therefore, slope position significantly influenced SOC (<italic>p</italic> &lt; 0.001). Upper slope locations are more prone to soil erosion caused by gravity and surface runoff, leading to the removal of organic-rich topsoil. Eroded materials, including OM, are carried downhill and deposited in lower slope locations. This buildup increases SOC levels in certain locations (<xref ref-type="bibr" rid="B43">Lal, 2003</xref>). In addition, increased SOC in the lower slope may be attributed to effective soil conservation practices, reduced human intervention, decreased grazing pressure, the regeneration of native vegetation, and partially enclosed practices (<xref ref-type="bibr" rid="B10">Bahadur Budha et&#xa0;al., 2020</xref>; <xref ref-type="bibr" rid="B6">Ali, 2022</xref>).</p>
</sec>
<sec id="s3_2_3">
<label>3.2.3</label>
<title>Total nitrogen</title>
<p>Land use showed a significant difference (<italic>p</italic> &lt; 0.001) in relation to TN. The highest TN value (0.41) was recorded in cultivated land, while the lowest (0.21) was observed in forestry land (<xref ref-type="table" rid="T2">
<bold>Table&#xa0;2</bold>
</xref>). Higher TN levels on cultivated land result from farmers&#x2019; frequent application of synthetic nitrogen fertilizers (such as urea and ammonium sulfate) to increase crop productivity. In some cases, crop residues are left on fields and plowed back into the soil, contributing to nitrogen enrichment (<xref ref-type="bibr" rid="B17">Brady and Weil, 2008</xref>). The lowest TN content is observed in forestry land, and the decomposition of organic material is slower due to cooler microclimates and reduced microbial activity, leading to lower nitrogen mineralization (<xref ref-type="bibr" rid="B53">Sandhya et&#xa0;al., 2023</xref>). In addition, various authors have suggested that in forest land, nitrogen is often immobilized in plant biomass and released slowly over time. Furthermore, deep-rooted trees may contribute to greater nitrogen leaching, reducing the available TN in the soil (<xref ref-type="bibr" rid="B57">Shibu Ebrahim, 2007</xref>; <xref ref-type="bibr" rid="B53">Sandhya et&#xa0;al., 2023</xref>).</p>
<p>TN significantly influenced slope position (<xref ref-type="table" rid="T2">
<bold>Table&#xa0;2</bold>
</xref>). Therefore, the highest TN was registered in the lowest part of the slope position. OM and nutrients are moved from higher to lower slope locations via soil erosion processes. Fine soil particles that are high in OM and nitrogen are carried downslope by runoff, where they accumulate at the lower slope (<xref ref-type="bibr" rid="B22">Demeku et&#xa0;al., 2022</xref>; <xref ref-type="bibr" rid="B9">Atinafu et&#xa0;al., 2024</xref>). Moreover, the favorable moisture conditions in lower slope positions promote suitability to vegetation growth. The resulting plant litter and root biomass contribute additional OM, which, upon activity decomposition, increases the soil&#x2019;s nitrogen content (<xref ref-type="bibr" rid="B39">Kidanemariam et&#xa0;al., 2012</xref>; <xref ref-type="bibr" rid="B54">Seifu et&#xa0;al., 2020</xref>).</p>
</sec>
<sec id="s3_2_4">
<label>3.2.4</label>
<title>Available phosphorus</title>
<p>The average value of Av. P was 3.6, 4.64, and 2.9 ppm in forestry, enset, and cultivated land use, respectively. Hence, Av. P was significantly varied (<italic>p</italic> &lt; 0.001) in land use. The highest Av. P was recorded in enset land, and this may be related to long-term manure, appropriate handling of the leftovers, household waste refusal of the application and increased soil Av. P concentration, and the resulting rise in microbiological activity. Similarly (<xref ref-type="bibr" rid="B46">Materechera, 2001</xref>), the report has also documented how OM increases the mobility of inorganic P and complexes P from adsorption sites in ligand exchange, influencing P in soil solution. This result confirms (<xref ref-type="bibr" rid="B5">Alemayehu and Sheleme, 2013</xref>) that Av. P may have risen as a result of the conversion of organic P to accessible P through mineralization, the addition of crop manure and animal debris to enset crop, and the coating of the reaction surfaces of the soil particles to prevent or delay P sorption.</p>
<p>In slope position, Av. P in the lower and middle part of the slope positions was higher, and Av. P content in the lower slope was found to be higher than that of the upper slope. Besides, it is significantly affected at the slope position. This could be due to the application of manure and phosphorus fertilizers on lower slope farm, as well as the deposition of eroded material such as crop residual and animal debris from upper slope. These factors might contribute to the increased availability of phosphorus (Av.p) in lower slope area.</p>
</sec>
</sec>
<sec id="s3_3">
<label>3.3</label>
<title>Soil organic carbon stocks</title>
<p>The result showed that SOCS vary in forestry, cultivated, and enset land-use practices; the highest mean registered was 95.52 and 94.73 mg ha<sup>&#x2212;1</sup> in forestry land and enset land, respectively. Moreover, SOCS significantly varied in forestry, cultivated, and enset land. When comparing enset land, Forestry and cultivated land use, Forestry land had the highest SOCS. The natural forest&#x2019;s larger SOCS may be explained by the regular input of litter, the existence of a root system, and the altered microclimate, all of which slow down the pace at which OM decomposes. Similarly (<xref ref-type="bibr" rid="B30">Girma et&#xa0;al., 2020</xref>; <xref ref-type="bibr" rid="B38">Kibet et&#xa0;al., 2022</xref>), the higher SOCS registered for the soil of the forest land could be mainly because of the low rate of litter decay, and biomass inputs also found a higher mean SOCS in the virgin forest than in all the other land cover categories in the western part of Ethiopia (<xref ref-type="bibr" rid="B45">Lemenih et&#xa0;al., 2006</xref>). The lowest SOCS were recorded in cultivated land because there is little nutrient replenishment. Cultivated land soils are characterized by low nutrient levels along with deterioration; this might be due to frequent cultivation and low nutrient replenishment, which caused the soil aggregate to disintegrate and release the available OC into the atmosphere (<xref ref-type="bibr" rid="B45">Lemenih et&#xa0;al., 2006</xref>; <xref ref-type="bibr" rid="B30">Girma et&#xa0;al., 2020</xref>; <xref ref-type="bibr" rid="B38">Kibet et&#xa0;al., 2022</xref>). Furthermore, the reduction of crop biomass after harvesting may be the primary cause of the low SOCS. Different field research studies indicated that in the majority of the soils in Ethiopia&#x2019;s Bale highlands, rainfall and clay content are the main factors regulating SOCS under various land uses by influencing the rates of initial production and breakdown (<xref ref-type="bibr" rid="B19">Chibsa and Ta&#x2019;, 2009</xref>).</p>
<p>In the case of slope position, the highest SOCS was 87.03 mg ha<sup>&#x2212;1</sup>, and the lowest was 73.38 mg ha<sup>&#x2212;1</sup> in upper and lower slope locations, respectively. The mean SOCS content showed differences in the following order: lower &gt; middle &gt; upper slope position.</p>
</sec>
<sec id="s3_4">
<label>3.4</label>
<title>The effects of land use and slope position response on cation exchange capacity and exchangeable base</title>
<p>Relatively, the highest CEC was observed under enset land use and the lowest CEC was recorded in cultivated land. Hence, CEC significantly varied in forestry, enset, and cultivated land. Therefore, the highest CEC observed land might be land in terms of crop residue, plant coverage, and tree canopy that are linked to an increase in CEC in enset land. This result confirms (<xref ref-type="bibr" rid="B5">Alemayehu and Sheleme, 2013</xref>) that an increase in OM content was the primary cause of the increase in soil CEC values in agricultural land uses. The lowest CEC was recorded in cultivated land when compared with other lands; this could be due to the cleaning of crop residual and intensive cultivation, which might be linked to the decline of OC, which is a result of decreasing CEC (<xref ref-type="bibr" rid="B6">Ali, 2022</xref>).</p>
<p>In the case of slope positions, the CEC ranges from 44.11 to 27.94, which was recorded at the lower and middle slope positions (<xref ref-type="table" rid="T3">
<bold>Table&#xa0;3</bold>
</xref>). The slope position significantly influenced (<italic>p</italic> &lt; 0.001) CEC. The highest CEC was recorded in the lower slope position, and this could be attributed to the effect of the soil management practices that enhanced the OM content of the lower slope position since OM is significantly related to CEC according to the discussion (<xref ref-type="bibr" rid="B56">Sheleme, 2017</xref>). In general, the CEC of the land use and slope position was medium to high (<xref ref-type="bibr" rid="B44">Landon, 1991</xref>).</p>
<table-wrap id="T3" position="float">
<label>Table&#xa0;3</label>
<caption>
<p>Effects of land use and slope position on CEC and exchangeable base of soil.</p>
</caption>
<table frame="hsides">
<thead>
<tr>
<th valign="middle" align="left">Land use</th>
<th valign="middle" align="left">CEC</th>
<th valign="top" align="left">Ca<sup>2+</sup>
</th>
<th valign="middle" align="left">Mg<sup>2+</sup>
</th>
<th valign="middle" align="left">K<sup>+</sup>
</th>
<th valign="middle" align="left">Na<sup>+</sup>
</th>
</tr>
</thead>
<tbody>
<tr>
<th valign="top" align="left" colspan="6">Cmol (+/kg)</th>
</tr>
<tr>
<td valign="top" align="left">Forestry</td>
<td valign="top" align="left">36.9<sup>a</sup>
</td>
<td valign="top" align="left">10.8<sup>a</sup>
</td>
<td valign="top" align="left">6.4<sup>a</sup>
</td>
<td valign="top" align="left">4.78<sup>a</sup>
</td>
<td valign="top" align="left">0.58<sup>a</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">Enset</td>
<td valign="top" align="left">37.22<sup>a</sup>
</td>
<td valign="top" align="left">9.9<sup>a</sup>
</td>
<td valign="top" align="left">5.9<sup>a</sup>
</td>
<td valign="top" align="left">2.5<sup>b</sup>
</td>
<td valign="top" align="left">0.48<sup>b</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">Cultivated</td>
<td valign="top" align="left">31.17<sup>b</sup>
</td>
<td valign="top" align="left">5.74<sup>b</sup>
</td>
<td valign="top" align="left">3.3<sup>b</sup>
</td>
<td valign="top" align="left">1.11<sup>c</sup>
</td>
<td valign="top" align="left">0.46<sup>b</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">CV (%)</td>
<td valign="top" align="left">4.74</td>
<td valign="top" align="left">13.53</td>
<td valign="top" align="left">11.63</td>
<td valign="top" align="left">10.86</td>
<td valign="top" align="left">15.39</td>
</tr>
<tr>
<td valign="top" align="left">LSD</td>
<td valign="top" align="left">0.24</td>
<td valign="top" align="left">0.97</td>
<td valign="top" align="left">0.64</td>
<td valign="top" align="left">0.25</td>
<td valign="top" align="left">0.07</td>
</tr>
<tr>
<td valign="top" align="left">
<italic>p</italic>-value</td>
<td valign="top" align="left">*</td>
<td valign="top" align="left">*</td>
<td valign="top" align="left">*</td>
<td valign="top" align="left">***</td>
<td valign="top" align="left">*</td>
</tr>
<tr>
<th valign="top" colspan="6" align="center">Slope</th>
</tr>
<tr>
<td valign="top" align="left">5&#x2013;10</td>
<td valign="top" align="left">44.11<sup>a</sup>
</td>
<td valign="top" align="left">10.1<sup>a</sup>
</td>
<td valign="top" align="left">5.53<sup>a</sup>
</td>
<td valign="top" align="left">4.06<sup>a</sup>
</td>
<td valign="top" align="left">0.62<sup>a</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">10&#x2013;15</td>
<td valign="top" align="left">27.94<sup>c</sup>
</td>
<td valign="top" align="left">9.2<sup>a</sup>
</td>
<td valign="top" align="left">5.73<sup>a</sup>
</td>
<td valign="top" align="left">2.33<sup>b</sup>
</td>
<td valign="top" align="left">0.48<sup>b</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">&gt;15</td>
<td valign="top" align="left">33.23<sup>b</sup>
</td>
<td valign="top" align="left">7.2<sup>b</sup>
</td>
<td valign="top" align="left">4.33<sup>b</sup>
</td>
<td valign="top" align="left">1.99<sup>c</sup>
</td>
<td valign="top" align="left">0.43<sup>b</sup>
</td>
</tr>
<tr>
<td valign="top" align="left">LSD</td>
<td valign="top" align="left">2.91</td>
<td valign="top" align="left">0.97</td>
<td valign="top" align="left">0.64</td>
<td valign="top" align="left">0.25</td>
<td valign="top" align="left">0.06</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn>
<p>
<italic>*</italic>Interaction effect means within a row (land use with soil depth of a given parameter) followed by the same letter(s) are not significantly different from each other at <italic>p</italic> &#x2264; 0.05. LSD, Least significant difference.</p>
</fn>
<fn>
<p>*- significant difference. ***- highly significant difference.</p>
</fn>
</table-wrap-foot>
</table-wrap>
<p>The exchangeable base was dominated by Ca followed by Mg, K, and Na. The highest Ca was observed in forestry land (<xref ref-type="table" rid="T3">
<bold>Table&#xa0;3</bold>
</xref>). Furthermore, the lowest content of Ca<sup>2+</sup> was recorded in cultivated land. Because of their absorption, low plant nutrient replenishment, and high leaching intensity in cultivated land, exchangeable Ca<sup>2+</sup> has been decreased in recently developed cultivated soils. This significantly reduces soil quality. Therefore, exchangeable Ca significantly (<italic>p</italic> &gt; 0.005) affected forestry, enset, and cultivated land use. The exchangeable magnesium (Mg) concentrations followed a similar trend to that of Ca under different land-use systems. The highest exchangeable Mg 6.4 and 5.9 Cmol (+/kg) receded in forestry and enset, respectively (<xref ref-type="table" rid="T3">
<bold>Table&#xa0;3</bold>
</xref>).</p>
<p>Variations in source substances, slope gradients, altitude, OM satisfaction, clay content, and ways to manage soil might be contributing factors for variations in exchangeable basic cation content among farms (<xref ref-type="bibr" rid="B29">Geremu et&#xa0;al., 2021</xref>). The highest exchangeable Ca<sup>2+</sup>, Mg<sup>2+</sup>, and K<sup>+</sup> are significantly higher in forestry land use. This is related to the idea (<xref ref-type="bibr" rid="B23">Desalegn, 2024</xref>) that high soil OM acts as a nutrient storage and reduces soil erosion; it may be the cause of the greater exchangeable base concentrations observed with relatively higher OM. This could also help limit the leaching of basic cations from the soil.</p>
<p>The relatively low concentrations of K<sup>+</sup> and Na<sup>+</sup> were recorded in cultivated land (<xref ref-type="table" rid="T3">
<bold>Table&#xa0;3</bold>
</xref>). This is attributed to the lower organic matter (OM) content, intensive cultivation practices, and reduced animal and crop residues, which contribute to the decline in these nutrient levels.</p>
</sec>
</sec>
<sec id="s4" sec-type="conclusions">
<label>4</label>
<title>Conclusion</title>
<p>This study contributes to the existing body of knowledge by demonstrating how sustainable land-use systems (forestry and enset) enhance SOCS and improve soil fertility, thereby mitigating climate change through carbon sequestration. By comparing different slope positions and land uses, we provide empirical evidence that forestry and perennial cropping systems (enset) significantly reduce soil nutrient loss and increase SOC retention compared to conventional cultivation, highlighting their role in low-emission land management. The study highlights significant variations in soil physical and chemical properties across different land uses and slope positions. This study quantitatively demonstrates that forestry and enset land-use systems significantly enhance SOCS (95.52 and 94.73 Mg ha<sup>&#x2212;</sup>&#xb9;, respectively), improve soil fertility [CEC: 36.9&#x2013;37.22 Cmol(+)/kg], and reduce bulk density (1.00&#x2013;1.03 g cm<sup>&#x2212;</sup>&#xb3;) compared to cultivated lands [SOCS: 54.68 Mg ha<sup>&#x2212;</sup>&#xb9;, CEC: 31.17 Cmol(+)/kg, BD: 1.13 g cm<sup>&#x2212;</sup>&#xb3;]. Slope position further modulates these effects, with lower slopes accumulating higher clay (60.89%), SOC (4.67%), and TN (0.40%) due to erosion&#x2013;deposition dynamics.</p>
<p>These findings emphasize the critical need for sustainable land management practices that preserve vegetation cover, reduce soil erosion, and enhance OM to mitigate soil degradation and improve agricultural productivity across varied landscapes.</p>
</sec>
</body>
<back>
<sec id="s5" sec-type="data-availability">
<title>Data availability statement</title>
<p>The original contributions presented in the study are included in the article/supplementary material. Further inquiries can be directed to the corresponding author.</p>
</sec>
<sec id="s6" sec-type="author-contributions">
<title>Author contributions</title>
<p>CM: Writing &#x2013; original draft, Writing &#x2013; review &amp; editing. AJ: Supervision, Visualization, Writing &#x2013; review &amp; editing.</p>
</sec>
<sec id="s7" sec-type="funding-information">
<title>Funding</title>
<p>The author(s) declare that no financial support was received for the research and/or publication of this article.</p>
</sec>
<ack>
<title>Acknowledgments</title>
<p>The authors are grateful to the Ministry of Science and Higher Education and Oda Bultum University for facilitating the study and research work. We are thankful to Essayas Assefa for the assistance and experiences shared during data collection.</p>
</ack>
<sec id="s8" sec-type="COI-statement">
<title>Conflict of interest</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec id="s9" sec-type="ai-statement">
<title>Generative AI statement</title>
<p>The author(s) declare that no Generative AI was used in the creation of this manuscript.</p>
</sec>
<sec id="s10" sec-type="disclaimer">
<title>Publisher&#x2019;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
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