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<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Aging Neurosci.</journal-id>
<journal-title>Frontiers in Aging Neuroscience</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Aging Neurosci.</abbrev-journal-title>
<issn pub-type="epub">1663-4365</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="doi">10.3389/fnagi.2025.1666449</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Neuroscience</subject>
<subj-group>
<subject>Systematic Review</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>The effects of Nordic walking on cognitive function in older adults: a systematic review and meta-analysis</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author" equal-contrib="yes">
<name><surname>Li</surname> <given-names>Haobai</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="author-notes" rid="fn002"><sup>&#x2020;</sup></xref>
<xref ref-type="author-notes" rid="fn004"><sup>&#x2021;</sup></xref>
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<contrib contrib-type="author" equal-contrib="yes">
<name><surname>Zhu</surname> <given-names>Ke</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="author-notes" rid="fn002"><sup>&#x2020;</sup></xref>
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<contrib contrib-type="author" corresp="yes">
<name><surname>Gan</surname> <given-names>Jianyu</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="corresp" rid="c001"><sup>&#x002A;</sup></xref>
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<contrib contrib-type="author">
<name><surname>Wang</surname> <given-names>Ziyi</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
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<contrib contrib-type="author">
<name><surname>Gao</surname> <given-names>Zhikun</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
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<contrib contrib-type="author">
<name><surname>Liu</surname> <given-names>Liangru</given-names></name>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref>
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<contrib contrib-type="author">
<name><surname>Guo</surname> <given-names>Xiaojie</given-names></name>
<xref ref-type="aff" rid="aff3"><sup>3</sup></xref>
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<contrib contrib-type="author" corresp="yes">
<name><surname>Niu</surname> <given-names>Jianfeng</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="corresp" rid="c002"><sup>&#x002A;</sup></xref>
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<aff id="aff1"><sup>1</sup><institution>Sports Coaching College, Beijing Sport University</institution>, <addr-line>Beijing</addr-line>, <country>China</country></aff>
<aff id="aff2"><sup>2</sup><institution>School of Physical Education, Guangxi University</institution>, <addr-line>Guangxi</addr-line>, <country>China</country></aff>
<aff id="aff3"><sup>3</sup><institution>Sports Department, Nankai University</institution>, <addr-line>Tianjin</addr-line>, <country>China</country></aff>
<author-notes>
<fn fn-type="edited-by"><p>Edited by: <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/47001/overview">Yih-Kuen Jan</ext-link>, University of Illinois Urbana-Champaign, United States</p></fn>
<fn fn-type="edited-by"><p>Reviewed by: <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/527246/overview">Alexandra Wolf</ext-link>, RIKEN Center for Advanced Intelligence Project (AIP), Japan</p>
<p><ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/809229/overview">Morteza Taheri</ext-link>, University of Tehran, Iran</p>
<p><ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/2321875/overview">Hamza K&#x00FC;&#x00E7;&#x00FC;k</ext-link>, Ondokuz May &#x0131; s University, T&#x00FC;rkiye</p></fn>
<corresp id="c001">&#x002A;Correspondence: Jianfeng Niu, <email>787451087@qq.com</email></corresp>
<corresp id="c002">Jianyu Gan, <email>2019010250@bsu.edu.cn</email></corresp>
<fn fn-type="equal" id="fn002"><p><sup>&#x2020;</sup>These authors have contributed equally to this work and shared the first authorship</p></fn>
<fn fn-type="other" id="fn004"><p><sup>&#x2021;</sup>ORCID: Haobai Li, <ext-link ext-link-type="uri" xlink:href="https://orcid.org/0009-0009-2559-1453">orcid.org/0009-0009-2559-1453</ext-link></p></fn>
</author-notes>
<pub-date pub-type="epub">
<day>17</day>
<month>09</month>
<year>2025</year>
</pub-date>
<pub-date pub-type="collection">
<year>2025</year>
</pub-date>
<volume>17</volume>
<elocation-id>1666449</elocation-id>
<history>
<date date-type="received">
<day>17</day>
<month>07</month>
<year>2025</year>
</date>
<date date-type="accepted">
<day>03</day>
<month>09</month>
<year>2025</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#x00A9; 2025 Li, Zhu, Gan, Wang, Gao, Liu, Guo and Niu.</copyright-statement>
<copyright-year>2025</copyright-year>
<copyright-holder>Li, Zhu, Gan, Wang, Gao, Liu, Guo and Niu</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/"><p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p></license>
</permissions>
<abstract>
<sec>
<title>Objectives</title>
<p>Nordic walking (NW), as a specialized form of aerobic exercise, emerges as a promising strategy to improve the cognitive function in older population. However, the effectiveness of NW has yet to be definitively confirmed due to the variances in the study designs and observations. This systematic review and meta-analysis was thus conducted to examine the effect of NW interventions on cognitive function of older adults.</p>
</sec>
<sec>
<title>Methods</title>
<p>The search was conducted in August 2025 on Web of Science, PubMed, SPORT-Discus, Medline, the Cochrane Library, Scopus, and PsycINFO databases. Two reviewers independently reviewed the search results, extracted the data, and assessed the risk of bias and certainty of evidence. Meta-analyses and meta-regressions were performed to determine the overall effect size and the impact of potential moderators.</p>
</sec>
<sec>
<title>Results</title>
<p>Initial screening identified 336 records, and after full-text assessment, eight studies (from 2014 to 2024) comprising 327 participants (71.19 &#x00B1; 5.44 yrs) were included. The effect size of NW on executive function was significant [Hedges&#x2019; <italic>g</italic> = 0.89, 95% CI (0.27, 1.50), <italic>p</italic> = 0.01], while the effects were non-significant for global function, memory function, attention, information processing, and perceptual ability (<italic>p</italic> &#x003E; 0.05). Subgroup analysis indicated that the health conditions of participants and the types of control groups significantly moderated executive function. Specifically, NW showed significant improvements (i) in older adults with health conditions and (ii) compared with inactive control groups (<italic>p</italic> = 0.04). Meta-regression revealed a significant positive correlation between the total intervention time of NW and its effect size (<italic>p</italic> &#x003C; 0.01).</p>
</sec>
<sec>
<title>Conclusion</title>
<p>This systematic review and meta-analysis demonstrates that NW interventions could improve executive function in older adults, especially those with health conditions.</p>
</sec>
<sec>
<title>Systematic review registration</title>
<p><uri xlink:href="https://www.crd.york.ac.uk/prospero">https://www.crd.york.ac.uk/prospero</uri>, identifier CRD42025638467.</p>
</sec>
</abstract>
<kwd-group>
<kwd>Nordic walking</kwd>
<kwd>exercise</kwd>
<kwd>cognitive function</kwd>
<kwd>older adults</kwd>
<kwd>meta-analysis</kwd>
</kwd-group>
<counts>
<fig-count count="4"/>
<table-count count="3"/>
<equation-count count="1"/>
<ref-count count="70"/>
<page-count count="14"/>
<word-count count="8465"/>
</counts>
<custom-meta-wrap>
<custom-meta>
<meta-name>section-at-acceptance</meta-name>
<meta-value>Neurocognitive Aging and Behavior</meta-value>
</custom-meta>
</custom-meta-wrap>
</article-meta>
</front>
<body>
<sec id="S1" sec-type="intro">
<title>Introduction</title>
<p>Aging is associated with progressive physiological changes, including reduced neurogenesis, impaired synaptic plasticity, and decreased cerebral perfusion in older adults (<xref ref-type="bibr" rid="B14">Castellano et al., 2017</xref>; <xref ref-type="bibr" rid="B56">Poulose et al., 2017</xref>; <xref ref-type="bibr" rid="B57">Raichlen and Alexander, 2017</xref>; <xref ref-type="bibr" rid="B29">Gonzales et al., 2022</xref>). These changes collectively contribute to cognitive decline, particularly affecting memory, executive function, and processing speed (<xref ref-type="bibr" rid="B9">Bettio et al., 2017</xref>; <xref ref-type="bibr" rid="B23">Fan et al., 2017</xref>; <xref ref-type="bibr" rid="B42">Leeuwis et al., 2017</xref>; <xref ref-type="bibr" rid="B35">Huang et al., 2022</xref>). The resultant cognitive decline in the older population leads to profound adverse consequences, including a marked deterioration in quality of life, increased dependency on caregiving support, and a higher risk of neurodegenerative diseases, particularly Alzheimer&#x2019;s disease (<xref ref-type="bibr" rid="B43">Li et al., 2017</xref>; <xref ref-type="bibr" rid="B58">S&#x00E1;ez De Asteasu et al., 2017</xref>). Given these impacts, developing effective interventions to maintain or improve cognitive function in older adults remains essential.</p>
<p>Current approaches against cognitive decline primarily encompass pharmacological interventions, cognitive training paradigms, and lifestyle modifications (<xref ref-type="bibr" rid="B63">Srikanth et al., 2020</xref>; <xref ref-type="bibr" rid="B8">Barnes et al., 2023</xref>; <xref ref-type="bibr" rid="B4">Antonenko et al., 2024</xref>; <xref ref-type="bibr" rid="B24">Faraziani and Eken, 2024</xref>). While these strategies demonstrate some efficacy, they are constrained by some limitations. Specifically, pharmacological interventions are frequently associated with adverse side effects and potential long-term complications, raising concerns about their safety profile and sustainability (<xref ref-type="bibr" rid="B52">Parnetti et al., 1997</xref>; <xref ref-type="bibr" rid="B10">Blackman et al., 2021</xref>; <xref ref-type="bibr" rid="B66">Van Dyck et al., 2023</xref>). Cognitive training approaches, though theoretically promising, often exhibit limited ecological validity and practical applicability, with questionable generalizability to real-world cognitive functions (<xref ref-type="bibr" rid="B5">Bahar-Fuchs et al., 2013</xref>; <xref ref-type="bibr" rid="B12">Butler et al., 2018</xref>). Lifestyle modifications face challenges in implementation and long-term adherence, particularly in elderly populations with varying health conditions and functional capacities (<xref ref-type="bibr" rid="B40">Knight et al., 2016</xref>; <xref ref-type="bibr" rid="B7">Barber et al., 2023</xref>). These limitations highlight the need for developing alternative intervention strategies that are not only efficacious but also characterized by enhanced safety, accessibility, and sustainability.</p>
<p>Recent literature increasingly corroborates that exercise is a particularly promising intervention strategy (<xref ref-type="bibr" rid="B59">Sanders et al., 2020</xref>; <xref ref-type="bibr" rid="B70">Zhang et al., 2023</xref>; <xref ref-type="bibr" rid="B32">Hatami et al., 2025</xref>). Exercise has been shown to promote neuroplasticity and regulate inflammatory processes, thereby creating an optimal neurobiological environment for cognitive preservation and enhancement in older populations (<xref ref-type="bibr" rid="B34">Hortob&#x00E1;gyi et al., 2022</xref>; <xref ref-type="bibr" rid="B68">Vints et al., 2024</xref>; <xref ref-type="bibr" rid="B41">Lavie et al., n.d.</xref>). Among various forms of exercise, walking is the preferred choice for most older adults to enhance their cognitive function due to its safety and low intensity, especially for those with limited physical function associated with aging or disease (<xref ref-type="bibr" rid="B1">Adderley et al., 2025</xref>; <xref ref-type="bibr" rid="B2">Ahmadpour et al., 2025</xref>; <xref ref-type="bibr" rid="B17">Cunha et al., 2025</xref>; <xref ref-type="bibr" rid="B60">Sandroff et al., 2025</xref>). Notably, recent research has shown that compared with standard walking, a form of walking known as Nordic walking (NW) is less physically demanding for older adults and may provide greater cognitive benefits (<xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>; <xref ref-type="bibr" rid="B49">Nemoto et al., 2021</xref>; <xref ref-type="bibr" rid="B38">Kettinen et al., 2023</xref>). NW distinguishes itself through the incorporation of two specially designed poles that facilitate active engagement of the upper body musculature, thereby resulting in more propulsion and energy expenditure (<xref ref-type="bibr" rid="B61">Schiffer et al., 2006</xref>). The arm-swinging motion involved in NW is beneficial for maintaining coordination of upper and lower limbs, which may generate similar cognitive effects to dual-task walking (<xref ref-type="bibr" rid="B19">Doi et al., 2014</xref>; <xref ref-type="bibr" rid="B25">Franzoni et al., 2018</xref>; <xref ref-type="bibr" rid="B28">Gome&#x00F1;uka et al., 2019</xref>). Studies have demonstrated that NW induced significant improvements in executive function and memory of older adults with or without health conditions as compared to walking or blank control groups (<xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>; <xref ref-type="bibr" rid="B30">Guszkowska et al., 2022</xref>; <xref ref-type="bibr" rid="B38">Kettinen et al., 2023</xref>; <xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref>). However, despite these promising preliminary results, existing evidence remains inconsistent. Several studies reported that no significant changes in cognitive function were observed following NW intervention (<xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>; <xref ref-type="bibr" rid="B31">Haas et al., 2024</xref>). These inconsistencies may stem from variations in study design, such as intervention duration, intensity, or participant characteristics. Additionally, the lack of standardized protocols for NW and the heterogeneity in cognitive function assessment metrics further complicate the interpretation of study findings.</p>
<p>Therefore, to highlight the recent study findings and explicitly and comprehensively examine the effects of NW on cognitive function in older adults and the potential contributors to such effects, we completed a systematic review and meta-analysis based upon up-to-date peer-reviewed publications. This work will ultimately provide critical knowledge to inform the appropriate intervention design in future research and rehabilitative practice for the maintenance of cognitive function in older populations.</p>
</sec>
<sec id="S2" sec-type="materials|methods">
<title>Materials and methods</title>
<sec id="S2.SS1">
<title>Study protocol</title>
<p>This systematic review and meta-analysis was conducted using Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines (<xref ref-type="bibr" rid="B51">Page et al., 2021</xref>) and registered with PROSPERO (Registration ID: CRD42025638467), an international prospective registry for systematic reviews.</p>
</sec>
<sec id="S2.SS2">
<title>Literature search</title>
<p>Two authors (HL and JG) independently searched Web of Science, PubMed, MEDLINE, SPORT-Discus, Cochrane Library, Scopus, and PsycINFO from inception to August 5, 2025; Studies were searched in the electronic databases using the following key terms combined by Boolean logic (&#x201C;AND&#x201D;, &#x201C;OR&#x201D;): (&#x201C;Nordic walking&#x201D; OR &#x201C;Nordic pole walking&#x201D; OR &#x201C;pole walking&#x201D;) AND (&#x201C;Cognitive function&#x201D; OR &#x201C;cognition&#x201D; OR &#x201C;Cognitive performance&#x201D;). A secondary search strategy was also used, which involved a manual search in the reference lists of eligible studies (i.e., citation tracking). Searches were limited to publications in English. Any disagreements arising during this process were resolved through discussion between the two authors (HL and JG), with additional input provided by a third author (JN). Detailed search strategies are provided in <xref ref-type="supplementary-material" rid="TS1">Supplementary Table 1</xref>.</p>
</sec>
<sec id="S2.SS3">
<title>Selection criteria</title>
<p>All included studies must be published articles. The inclusion criteria were carried out according to the PICOS principle: (1) Population: All the participants included in this study were at least 60 years old. None of them had used any drugs known to significantly affect cognitive function, or had discontinued such drugs for more than 4 weeks, or had indicated their actual medication status at baseline to ensure the comparability of drug exposure between the two groups; (2) Interventions: the interventions used were NW only or NW combined with other interventions. When NW was used in combination with other interventions, the control group was supposed to receive other interventions alone to ensure that the observed changes were caused by NW; (3) Comparisons: Each group is characterized as either active (e.g., interventions involving physical activities other than Nordic walking, NW) or inactive (e.g., non-intervention, or routine treatment for the diseases the subjects suffer from); (4) Outcomes: Outcome measures reflecting cognitive function were employed. (5) Study design: The study employed randomized controlled trials or randomized crossover trial designs. Articles with the following conditions will be excluded: (1) did not investigate cognitive function outcomes or provide specific data of outcome measures (e.g., reporting only <italic>p</italic>-values without means/SDs); (2) review papers, conference abstracts, and articles; (3) those with duplicate publications; (4) non-English publications.</p>
</sec>
<sec id="S2.SS4">
<title>Data extraction</title>
<p>The process of data extraction was conducted independently by two authors (HL and JG) according to the Cochrane Collaboration Handbook. The extracted information of the publications included: study (authors, year), participants (age, sex, physical condition), grouping and sample size, interventions (type, frequency, number of sessions, duration of each session, duration of intervention), auxiliary means (e.g., Nordic walking poles or Hiking pole, etc.), and outcome measures. Any outcome measures on which the two authors disagreed were discussed with the other two authors (JN and KZ) until a consensus was achieved. For each study, extract the mean and standard deviation (SD) of the post-intervention indicator results. For studies that do not report changes in results before and after, or those presenting results in the form of &#x201C;Mean &#x00B1; SE/SEM (Standard Error/Standard Error of the Mean)&#x201D;, use the following formula for calculation (<xref ref-type="bibr" rid="B11">Borenstein et al., 2013</xref>):</p>
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</disp-formula>
<p>When the full-text article data were presented only in a figure format, WebPlotDigitizer (Ankit Rohatgi, 2019, V.4.2; WebPlotDigitizer, Pacifica, CA, USA) was used to extract the data from the figures. In the absence of any relevant data, the first author or the corresponding author of the article would be contacted via email to obtain the required data.</p>
</sec>
<sec id="S2.SS5">
<title>Quality assessment</title>
<p>The quality of the included studies was independently evaluated by two authors (HL and JG) according to the principles of the Physiotherapy Evidence Database (PEDro). The PEDro scale, specifically designed to assess the methodological quality of randomized controlled trials in physical interventions, is highly suitable for evaluating the studies in this research (<xref ref-type="bibr" rid="B18">de Morton, 2009</xref>; <xref ref-type="bibr" rid="B13">Cashin and McAuley, 2020</xref>). This scale examines crucial elements such as randomization, blinding, and allocation concealment, which are essential for ensuring the internal validity of the studies included in this systematic review and meta-analysis (<xref ref-type="bibr" rid="B13">Cashin and McAuley, 2020</xref>).</p>
<p>Specifically, the PEDro scale consists of 11 items, to which we were required to respond with &#x201C;no&#x201D; or &#x201C;yes&#x201D;. For each &#x201C;no&#x201D; response, a value of 0 was assigned, and for each &#x201C;yes&#x201D; response, a value of 1 was assigned. The total score for each study ranged from 0 to 11. Since blinding (especially of participants and investigators) is difficult to implement in exercise intervention trials (<xref ref-type="bibr" rid="B62">Sherrington et al., 2010</xref>), the methodological quality classification of each article was adjusted, taking into account the eligibility criteria as previously described [sum scores: &#x2265;6 (&#x201C;high quality, low risk of bias&#x201D;); scores: 4&#x2013;5 (&#x201C;acceptable quality, moderate risk of bias&#x201D;); scores: &#x2264; 3 (&#x201C;low quality, high risk of bias&#x201D;)].</p>
<p>The quality of the evidence was also independently appraised by two authors (HL and JG) based on the Grading of Recommendations Assessment, Development and Evaluation (GRADE) criteria. The GRADE criteria characterize the evidence in terms of study limitations, imprecision, inconsistency, indirectness, and publication bias. In cases where the two authors disagreed on any score, a third author (JN or KZ) was consulted for discussion until a consensus was reached.</p>
</sec>
<sec id="S2.SS6">
<title>Statistical analysis</title>
<p>Meta-analysis was carried out using Stata/MP 17.0 (STATA Corp, College Station, TX, USA) and R 4.2.0 (R Core Team, R Foundation for Statistical Computing, Vienna, Austria). Given the diverse measurement units of outcome measures across studies, such as time, score, number of stimuli, etc., a random-effects model was employed to calculate Hedges&#x2019; g and the 95% confidence interval (CI), which served as the indicator of the effect size for the difference in pre-post changes between the intervention and control groups (<xref ref-type="bibr" rid="B26">Fritz et al., 2012</xref>). The effect sizes were categorized as follows: trivial (Hedges&#x2019; <italic>g</italic> &#x003C; 0.2), small (0.2 &#x2264; Hedges&#x2019; <italic>g</italic> &#x003C; 0.5), moderate (0.5 &#x2264; Hedges&#x2019; <italic>g</italic> &#x003C; 0.8), or large (Hedges&#x2019; <italic>g</italic> &#x2265; 0.8) (<xref ref-type="bibr" rid="B16">Cohen, 2013</xref>). Additionally, prediction intervals were computed to reflect heterogeneity in comparison with confidence intervals. The between-study variance was estimated using the restricted maximum likelihood estimator with Hartung-Knapp adjustment (<xref ref-type="bibr" rid="B67">Veroniki et al., 2016</xref>). Statistical heterogeneity was evaluated by means of the heterogeneity chi-squared (&#x03C7;<sup>2</sup>) and I<sup>2</sup> values. The degree of heterogeneity was interpreted in accordance with the guidelines of the Cochrane Collaboration: 0%&#x2013;40% might not be of significance; 30%&#x2013;60% may indicate moderate heterogeneity; 50%&#x2013;90% may represent substantial heterogeneity; and 75%&#x2013;100% implies considerable heterogeneity (<xref ref-type="bibr" rid="B33">Higgins, 2003</xref>). In the event of substantial or considerable heterogeneity (I<sup>2</sup> &#x003E; 50%), subgroup analyses were conducted to explore the impact of study characteristics (e.g., the physical condition of the participants). Additionally, we performed meta-regression to explore the dose-response relationship of NW on cognitive function. The intervention duration, session number, and total time (i.e., session number &#x00D7; session duration) were used as the effect moderators. Subsequently, sensitivity analyses were performed to assess the stability of the pooled estimates and to determine whether any study influenced the overall effect size (<xref ref-type="bibr" rid="B67">Veroniki et al., 2016</xref>). Moreover, publication bias was assessed through the generation of funnel plots and the conduct of Egger&#x2019;s test. If significant asymmetry was detected, the Trim and Fill method was utilized to adjust for publication bias (<xref ref-type="bibr" rid="B20">Duval and Tweedie, 2000</xref>).</p>
</sec>
</sec>
<sec id="S3" sec-type="results">
<title>Results</title>
<sec id="S3.SS1">
<title>Study selection</title>
<p>The results of the study selection process are summarized in a PRISMA flowchart (<xref ref-type="fig" rid="F1">Figure 1</xref>). The study selection commenced with the identification of 336 records through database searches, which were distributed among Web of Science (<italic>n</italic> = 84), PubMed (<italic>n</italic> = 64), MEDLINE (<italic>n</italic> = 29), SPORT-Discus (<italic>n</italic> = 47), Cochrane Library (<italic>n</italic> = 46), Scopus (<italic>n</italic> = 61), and PsycINFO (<italic>n</italic> = 3). Additionally, two further studies were identified through citation searching. After removing 174 duplicate records, a total of 162 records were available for the screening phase. During the title and abstract screening stage, 143 records were excluded. Subsequently, a full-text eligibility assessment was conducted on 18 articles, and 10 studies were excluded. Eventually, 8 studies were included in the meta-analysis.</p>
<fig id="F1" position="float">
<label>FIGURE 1</label>
<caption><p>Flow diagram of the study selection process.</p></caption>
<alt-text>Flowchart depicting the identification and screening of studies via databases and citation searches. Initially 334 records were identified, with 174 duplicates excluded. After title and abstract screening, 143 records were excluded for reasons like irrelevant content. Seventeen articles underwent full-text assessment, resulting in eight being eligible. Additionally, two records were identified through citation searching, both undergoing screening but one was excluded due to missing data.</alt-text>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fnagi-17-1666449-g001.tif"/>
</fig>
</sec>
<sec id="S3.SS2">
<title>Characteristics of included studies</title>
<sec id="S3.SS2.SSS1">
<title>Participant characteristics</title>
<p>The eight studies included in the analysis were conducted across seven countries: Germany (<italic>n</italic> = 1), Poland (<italic>n</italic> = 1), Japan (<italic>n</italic> = 1), Thailand (<italic>n</italic> = 1), Brazil (<italic>n</italic> = 2), Italy (<italic>n</italic> = 1), and Finland (<italic>n</italic> = 1). The total number of participants across all studies was 327. Excluding one study that did not report age information (<xref ref-type="bibr" rid="B21">Ebersbach et al., 2014</xref>), the mean age of participants in the remaining seven studies was 70.92 years (<xref ref-type="table" rid="T1">Table 1</xref>). Six studies included both male and female participants (<xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>; <xref ref-type="bibr" rid="B47">Miyazaki et al., 2022</xref>; <xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>; <xref ref-type="bibr" rid="B38">Kettinen et al., 2023</xref>; <xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref>; <xref ref-type="bibr" rid="B31">Haas et al., 2024</xref>), one study exclusively involved female participants (<xref ref-type="bibr" rid="B30">Guszkowska et al., 2022</xref>), and one study did not report the gender distribution of participants (<xref ref-type="bibr" rid="B21">Ebersbach et al., 2014</xref>).</p>
<table-wrap position="float" id="T1">
<label>TABLE 1</label>
<caption><p>Characteristics of included studies (<italic>n</italic> = 8).</p></caption>
<table cellspacing="5" cellpadding="5" frame="box" rules="all">
<thead>
<tr>
<td valign="top" align="center">References</td>
<td valign="top" align="center">Sample size</td>
<td valign="top" align="center">Age</td>
<td valign="top" align="center">Sex men/women</td>
<td valign="top" align="center">Physical condition</td>
<td valign="top" align="center">Interventions</td>
<td valign="top" align="center">Duration</td>
<td valign="top" align="center">Frequency</td>
<td valign="top" align="center">Session number</td>
<td valign="top" align="center">Session duration</td>
<td valign="top" align="center">Outcome measures</td>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="center" rowspan="2"><xref ref-type="bibr" rid="B21">Ebersbach et al., 2014</xref></td>
<td valign="top" align="center">NW (19)</td>
<td valign="top" align="center" rowspan="2">Unclear</td>
<td valign="top" align="center" rowspan="2">Unclear</td>
<td valign="top" align="center" rowspan="2">Parkinson&#x2019;s disease</td>
<td valign="top" align="center">Nordic walking</td>
<td valign="top" align="center" rowspan="2">8 weeks</td>
<td valign="top" align="center" rowspan="2">2 times/week</td>
<td valign="top" align="center" rowspan="2">16</td>
<td valign="top" align="center" rowspan="2">60 min</td>
<td valign="top" align="center" rowspan="2">Information processing speed: cRT&#x2191;; nRT&#x2192;</td>
</tr>
<tr>
<td valign="top" align="center">CG (19)</td>
<td valign="top" align="center">Domestic exercise</td>
</tr>
<tr>
<td valign="top" align="center" rowspan="2"><xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref></td>
<td valign="top" align="center">NW (16)</td>
<td valign="top" align="center">64.9 &#x00B1; 10.2</td>
<td valign="top" align="center">13/3</td>
<td valign="top" align="center" rowspan="2">Parkinson&#x2019;s disease</td>
<td valign="top" align="center">Nordic walking</td>
<td valign="top" align="center" rowspan="2">9 weeks</td>
<td valign="top" align="center" rowspan="2">2 times/week</td>
<td valign="top" align="center" rowspan="2">18</td>
<td valign="top" align="center">The first 3 weeks: 35 min<break/> The last 6 weeks: 40&#x2013;60 min</td>
<td valign="top" align="center" rowspan="2">Global cognition: MoCA&#x2192;</td>
</tr>
<tr>
<td valign="top" align="center">CG (17)</td>
<td valign="top" align="center">70.5 &#x00B1; 5.8</td>
<td valign="top" align="center">7/10</td>
<td valign="top" align="center">Free walking</td>
</tr>
<tr>
<td valign="top" align="center" rowspan="2"><xref ref-type="bibr" rid="B30">Guszkowska et al., 2022</xref></td>
<td valign="top" align="center">NW (20)</td>
<td valign="top" align="center" rowspan="2">80.25 &#x00B1; 5.755</td>
<td valign="top" align="center">0/20</td>
<td valign="top" align="center" rowspan="2">Essential health</td>
<td valign="top" align="center">Nordic walking</td>
<td valign="top" align="center" rowspan="2">3 months</td>
<td valign="top" align="center" rowspan="2">2 times/week</td>
<td valign="top" align="center" rowspan="2">24</td>
<td valign="top" align="center" rowspan="2">60 min</td>
<td valign="top" align="center" rowspan="2">Information processing speed: APT (3/8) perception speed&#x2191;Perceptual Abilities: APT (3/8) perception fallibility&#x2191;Attention: APT (3/8) attention fallibility&#x2191;</td>
</tr>
<tr>
<td valign="top" align="center">CG (20)</td>
<td valign="top" align="center">0/20</td>
<td valign="top" align="center">No intervention</td>
</tr>
<tr>
<td valign="top" align="center" rowspan="2"><xref ref-type="bibr" rid="B47">Miyazaki et al., 2022</xref></td>
<td valign="top" align="center">NW (29)</td>
<td valign="top" align="center" rowspan="2">67.93 &#x00B1; 5.81</td>
<td valign="top" align="center">22/7</td>
<td valign="top" align="center" rowspan="2">Essential health</td>
<td valign="top" align="center">Nordic walking combined with a daily supplement containing 8 g of protein</td>
<td valign="top" align="center" rowspan="2">4 weeks</td>
<td valign="top" align="center" rowspan="2">3 times/week</td>
<td valign="top" align="center" rowspan="2">9&#x223C;12</td>
<td valign="top" align="center" rowspan="2">45 min</td>
<td valign="top" align="center" rowspan="2">Global cognition: MoCA&#x2192;Executive functions: FAB&#x2191;</td>
</tr>
<tr>
<td valign="top" align="center">CG (29)</td>
<td valign="top" align="center">23/6</td>
<td valign="top" align="center">Take a daily protein supplement containing about 8 grams of protein</td>
</tr>
<tr>
<td valign="top" align="center" rowspan="2"><xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref></td>
<td valign="top" align="center">NW (9)</td>
<td valign="top" align="center">78.89 &#x00B1; 6.68</td>
<td valign="top" align="center">3/6</td>
<td valign="top" align="center" rowspan="2">Alzheimer&#x2019;s disease</td>
<td valign="top" align="center">Nordic walking combined with reality orientation therapy, music therapy, motor, proprioceptive and postural rehabilitation</td>
<td valign="top" align="center" rowspan="2">24 weeks</td>
<td valign="top" align="center" rowspan="2">2 times/week</td>
<td valign="top" align="center" rowspan="2">48</td>
<td valign="top" align="center" rowspan="2">60 min</td>
<td valign="top" align="center" rowspan="2">Global cognition: MMSE&#x2192;Executive functions: FAB&#x2192;; SWCT-time&#x2191;memory: RVLT-I&#x2192;; RVLT-D&#x2191;Perceptual Abilities: CGD&#x2192;Attention: Attentional Matrices&#x2192;Fluid Intelligence: CPM&#x2191;</td>
</tr>
<tr>
<td valign="top" align="center">CG (13)</td>
<td valign="top" align="center">78.92 &#x00B1; 8.04</td>
<td valign="top" align="center">5/8</td>
<td valign="top" align="center">Reality orientation therapy, music therapy, motor, proprioceptive and postural rehabilitation</td>
</tr>
<tr>
<td valign="top" align="center" rowspan="2"><xref ref-type="bibr" rid="B38">Kettinen et al., 2023</xref></td>
<td valign="top" align="center">NW (25)</td>
<td valign="top" align="center" rowspan="2">69 &#x00B1; 4.4</td>
<td valign="top" align="center" rowspan="2">16/9</td>
<td valign="top" align="center" rowspan="2">Essential health</td>
<td valign="top" align="center">Nordic walking</td>
<td valign="top" align="center" rowspan="2">&#x2014;&#x2014;</td>
<td valign="top" align="center" rowspan="2">&#x2014;&#x2014;</td>
<td valign="top" align="center" rowspan="2">1</td>
<td valign="top" align="center" rowspan="2">&#x2014;&#x2014;</td>
<td valign="top" align="center" rowspan="2">Information processing speed: TMT-A&#x2191;Executive functions: TMT(B-A)&#x2191;; TMT-B&#x2191;</td>
</tr>
<tr>
<td valign="top" align="center">CG (25)</td>
<td valign="top" align="center">Walking</td>
</tr>
<tr>
<td valign="top" align="center" rowspan="2"><xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref></td>
<td valign="top" align="center">NW (17)</td>
<td valign="top" align="center">69.2 &#x00B1; 5.3</td>
<td valign="top" align="center">5/11</td>
<td valign="top" align="center" rowspan="2">Type 2 Diabetes</td>
<td valign="top" align="center">Aquatic Nordic walking</td>
<td valign="top" align="center" rowspan="2">12 weeks</td>
<td valign="top" align="center" rowspan="2">3 times/week</td>
<td valign="top" align="center" rowspan="2">36</td>
<td valign="top" align="center" rowspan="2">60 min</td>
<td valign="top" align="center" rowspan="2">Global cognition: MoCA&#x2191;; MMSE&#x2192;Executive functions: SCT&#x2192;; TMT-B&#x2192;</td>
</tr>
<tr>
<td valign="top" align="center">CG (17)</td>
<td valign="top" align="center">68.9 &#x00B1; 3.7</td>
<td valign="top" align="center">7/10</td>
<td valign="top" align="center">No intervention</td>
</tr>
<tr>
<td valign="top" align="center" rowspan="2"><xref ref-type="bibr" rid="B31">Haas et al., 2024</xref></td>
<td valign="top" align="center">NW (31)</td>
<td valign="top" align="center">67.87 &#x00B1; 11.2</td>
<td valign="top" align="center">23/8</td>
<td valign="top" align="center" rowspan="2">Parkinson&#x2019;s disease</td>
<td valign="top" align="center">Nordic walking</td>
<td valign="top" align="center" rowspan="2">12 weeks</td>
<td valign="top" align="center" rowspan="2">2 times/week</td>
<td valign="top" align="center" rowspan="2">24</td>
<td valign="top" align="center" rowspan="2">60 min</td>
<td valign="top" align="center" rowspan="2">Global cognition: MoCA&#x2192;</td>
</tr>
<tr>
<td valign="top" align="center">CG (21)</td>
<td valign="top" align="center">66.76 &#x00B1; 8.97</td>
<td valign="top" align="center">17/4</td>
<td valign="top" align="center">Deep-water exercise</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn><p>APT(3/8), Attention and Perceptivity Test, version 3/8; CGD, Copying Geometric Drawings; CPM, Raven&#x2019;s Colored Progressive Matrices; cRT, Cued reaction time; FAB: Frontal Assessment Battery; MoCA: Montreal Cognitive Assessment; MMSE: Mini Mental State Examination; nRT: noncued reaction time; RVLT-I, Rey&#x2019;s auditory Verbal Learning Test-Immediate Recall; RVLT-D, Rey&#x2019;s auditory Verbal Learning Test-Delayed Recall; SCT, Stroop Color test; SWCT-time, Stroop Word-Color Interference test; TMT-A, Trail Making Test part A; TMT-B, Trail Making Test part B; TMT (B-A), Trail Making Test part B-A; &#x2191;, Intervention significantly (<italic>p</italic> &#x003C; 0.05) improved the outcome compared with control; &#x2192;, Intervention induced no significant difference compared with control (<italic>p</italic> &#x003E; 0.05).</p></fn>
</table-wrap-foot>
</table-wrap>
<p>Participants in three studies were essential health (<xref ref-type="bibr" rid="B30">Guszkowska et al., 2022</xref>; <xref ref-type="bibr" rid="B47">Miyazaki et al., 2022</xref>; <xref ref-type="bibr" rid="B38">Kettinen et al., 2023</xref>), while the other five studies included participants with health conditions (<xref ref-type="bibr" rid="B21">Ebersbach et al., 2014</xref>; <xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>; <xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>; <xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref>; <xref ref-type="bibr" rid="B31">Haas et al., 2024</xref>), including Parkinson&#x2019;s disease (<italic>n</italic> = 3) (<xref ref-type="bibr" rid="B21">Ebersbach et al., 2014</xref>; <xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>; <xref ref-type="bibr" rid="B31">Haas et al., 2024</xref>), Type 2 diabetes (<italic>n</italic> = 1) (<xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref>), and Alzheimer&#x2019;s disease (<italic>n</italic> = 1) (<xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>).</p>
</sec>
<sec id="S3.SS2.SSS2">
<title>Intervention characteristics</title>
<p>Of the eight studies incorporated in this review, seven were conducted using a randomized controlled trial design (<xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>; <xref ref-type="bibr" rid="B49">Nemoto et al., 2021</xref>; <xref ref-type="bibr" rid="B30">Guszkowska et al., 2022</xref>; <xref ref-type="bibr" rid="B47">Miyazaki et al., 2022</xref>; <xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>; <xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref>; <xref ref-type="bibr" rid="B31">Haas et al., 2024</xref>), with the remaining study adopting a randomized crossover controlled trial design (<xref ref-type="bibr" rid="B38">Kettinen et al., 2023</xref>). The characteristics of the intervention measures are detailed in <xref ref-type="table" rid="T1">Table 1</xref>. Among the included studies, five studies independently employed NW as the intervention measure (<xref ref-type="bibr" rid="B21">Ebersbach et al., 2014</xref>; <xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>; <xref ref-type="bibr" rid="B38">Kettinen et al., 2023</xref>; <xref ref-type="bibr" rid="B31">Haas et al., 2024</xref>, <xref ref-type="bibr" rid="B31">2024</xref>). One study combined NW with daily protein supplementation (<xref ref-type="bibr" rid="B47">Miyazaki et al., 2022</xref>), while another study utilized aquatic NW as the intervention (<xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref>). Additionally, one study implemented a multimodal intervention approach, integrating NW with reality orientation therapy, music therapy, physical exercise, proprioceptive training, and postural rehabilitation (<xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>).</p>
<p>Regarding the control groups, three studies employed non-active control conditions (<xref ref-type="bibr" rid="B30">Guszkowska et al., 2022</xref>; <xref ref-type="bibr" rid="B47">Miyazaki et al., 2022</xref>; <xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref>), which included maintaining usual daily activities (<italic>n</italic> = 2) (<xref ref-type="bibr" rid="B30">Guszkowska et al., 2022</xref>; <xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref>) and nutritional supplementation (daily intake of 8g protein supplements) (<italic>n</italic> = 1) (<xref ref-type="bibr" rid="B47">Miyazaki et al., 2022</xref>). The remaining five studies utilized active control conditions (<xref ref-type="bibr" rid="B21">Ebersbach et al., 2014</xref>; <xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>; <xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>; <xref ref-type="bibr" rid="B38">Kettinen et al., 2023</xref>; <xref ref-type="bibr" rid="B31">Haas et al., 2024</xref>), which involved interventions such as domestic exercise (<italic>n</italic> = 1) (<xref ref-type="bibr" rid="B21">Ebersbach et al., 2014</xref>), deep-water exercise (<italic>n</italic> = 1) (<xref ref-type="bibr" rid="B31">Haas et al., 2024</xref>), regular walking (<italic>n</italic> = 2) (<xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>; <xref ref-type="bibr" rid="B47">Miyazaki et al., 2022</xref>), and a multimodal training program incorporating reality orientation therapy, physical exercise, and proprioceptive training (<italic>n</italic> = 1) (<xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>).</p>
<p>The duration of a single intervention session varied from 35 to 60 min. The frequencies of intervention included a single session (<italic>n</italic> = 1) (<xref ref-type="bibr" rid="B38">Kettinen et al., 2023</xref>), two sessions per week (<italic>n</italic> = 5) (<xref ref-type="bibr" rid="B21">Ebersbach et al., 2014</xref>; <xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>; <xref ref-type="bibr" rid="B30">Guszkowska et al., 2022</xref>; <xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>; <xref ref-type="bibr" rid="B31">Haas et al., 2024</xref>), and three sessions per week (<italic>n</italic> = 2) (<xref ref-type="bibr" rid="B47">Miyazaki et al., 2022</xref>; <xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref>).</p>
<p>The overall intervention period ranged from 1 day to 24 weeks, and the total number of interventions across the studies was between 1 and 36. Specifically, the intervention protocols varied across the studies: one studies involved 16 sessions over 8 weeks (<xref ref-type="bibr" rid="B21">Ebersbach et al., 2014</xref>), one study included 18 sessions over 9 weeks (<xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>), one study implemented 24 sessions over 3 months (<xref ref-type="bibr" rid="B30">Guszkowska et al., 2022</xref>), one study featured 9&#x2013;12 sessions over 4 weeks (<xref ref-type="bibr" rid="B47">Miyazaki et al., 2022</xref>), one study consisted of 48 sessions over 24 weeks (<xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>), one study implemented 24 sessions over 24 weeks (<xref ref-type="bibr" rid="B31">Haas et al., 2024</xref>), one study involved a single session conducted over 1 day (<xref ref-type="bibr" rid="B38">Kettinen et al., 2023</xref>), and one study provided 36 sessions over 12 weeks (<xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref>). Across all studies, participants received prior instruction on the knowledge and skills necessary for NW before commencing the interventions.</p>
</sec>
</sec>
<sec id="S3.SS3">
<title>Study outcomes</title>
<p>This meta-analysis included 23 effect sizes from 8 studies. These effect sizes encompassed multiple domains of cognitive ability, including global cognitive function (<xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>; <xref ref-type="bibr" rid="B47">Miyazaki et al., 2022</xref>; <xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>; <xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref>; <xref ref-type="bibr" rid="B31">Haas et al., 2024</xref>), memory function (<xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>), executive function (<xref ref-type="bibr" rid="B47">Miyazaki et al., 2022</xref>; <xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>; <xref ref-type="bibr" rid="B38">Kettinen et al., 2023</xref>; <xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref>), perceptual abilities (<xref ref-type="bibr" rid="B30">Guszkowska et al., 2022</xref>; <xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>), information processing speed (<xref ref-type="bibr" rid="B30">Guszkowska et al., 2022</xref>; <xref ref-type="bibr" rid="B38">Kettinen et al., 2023</xref>), and attention (<xref ref-type="bibr" rid="B21">Ebersbach et al., 2014</xref>; <xref ref-type="bibr" rid="B30">Guszkowska et al., 2022</xref>; <xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>). Specific cognitive tests administered and their corresponding domain classifications are detailed in <xref ref-type="supplementary-material" rid="TS1">Supplementary Table 3</xref>.</p>
</sec>
<sec id="S3.SS4">
<title>Effect of NW on cognitive function levels</title>
<p>The overall effect size for cognitive function was moderate and statistically significant [Hedges&#x2019; <italic>g</italic> = 0.56, 95% CI (0.29, 0.84), PI (&#x2212;0.57, 1.70), <italic>p</italic> &#x003C; 0.01, <xref ref-type="fig" rid="F2">Figure 2</xref>], with substantial heterogeneity (I<sup>2</sup> = 70.75%, <italic>p</italic> &#x003C; 0.01). Meta-regression analysis indicated no significant association between effect size and the number of intervention sessions (<italic>b</italic> = 0.042, <italic>p</italic> = 0.62, <xref ref-type="fig" rid="F3">Figure 3A</xref>), while it revealed a significant positive association between effect size and the total intervention duration (<italic>b</italic> = 0.0003, <italic>p</italic> = 0.049, <xref ref-type="fig" rid="F3">Figure 3B</xref>).</p>
<fig id="F2" position="float">
<label>FIGURE 2</label>
<caption><p>The pooled effect size of NW on cognitive function levels.</p></caption>
<alt-text>Forest plot displaying meta-analysis results for cognitive domains: attention, executive function, global cognition, information processing, memory, and perceptual ability. Individual study effect sizes are shown as blue squares with confidence intervals. Summary effect sizes per category are depicted as red diamonds. The overall effect size is represented as a green diamond at the bottom. Heterogeneity statistics and weights are listed alongside study details.</alt-text>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fnagi-17-1666449-g002.tif"/>
</fig>
<fig id="F3" position="float">
<label>FIGURE 3</label>
<caption><p>Dose-response curve. <bold>(A)</bold> Session number. <bold>(B)</bold> Total time.</p></caption>
<alt-text>Two scatter plots titled &#x201C;Meta-Regression&#x201D; show the standardized mean difference on the y-axis. The left plot has session number on the x-axis, ranging from 0 to 50, while the right plot has total time, ranging from 500 to 3000. Both plots feature a trend line with a shaded confidence interval, suggesting a slight positive correlation.</alt-text>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fnagi-17-1666449-g003.tif"/>
</fig>
<p>For specific cognitive abilities, the effect size for executive function was large and significant [Hedges&#x2019; <italic>g</italic> = 0.89, 95% CI (0.27, 1.50), <italic>p</italic> = 0.01], with substantial heterogeneity (I<sup>2</sup> = 73.04%, <italic>p</italic> &#x003C; 0.01). The effect size for memory function was moderate but not statistically significant [Hedges&#x2019; <italic>g</italic> = 0.77, 95% CI (&#x2212;0.86, 2.39), p = 0.11], with non-significant heterogeneity (I<sup>2</sup> = 0.00%, <italic>p</italic> = 0.68). The effect size for global cognition was moderate but not statistically significant [Hedges&#x2019; <italic>g</italic> = 0.73, 95% CI (&#x2212;0.08, 1.53), <italic>p</italic> = 0.07], with substantial heterogeneity (I<sup>2</sup> = 80.46%, <italic>p</italic> &#x003C; 0.01). The effect size for attention was small and not statistically significant [Hedges&#x2019; <italic>g</italic> = 0.20, 95% CI (&#x2212;0.48, 0.88), <italic>p</italic> = 0.41], with moderate heterogeneity (I<sup>2</sup> = 40.81%, <italic>p</italic> = 0.17). The effect size for information processing was trivial and not statistically significant [Hedges&#x2019; <italic>g</italic> = 0.18, 95% CI (&#x2212;2.57, 2.93), <italic>p</italic> = 0.56], with non-significant heterogeneity (I<sup>2</sup> = 7.50%, <italic>p</italic> = 0.30). The effect size for perceptual ability was trivial and not statistically significant [Hedges&#x2019; <italic>g</italic> = &#x2212;0.07, 95% CI (&#x2212;3.70, 3.56), <italic>p</italic> = 0.85], with non-significant heterogeneity (I<sup>2</sup> = 20.61%, <italic>p</italic> = 0.26). The funnel plot (<xref ref-type="fig" rid="F4">Figure 4</xref>) and Egger&#x2019;s test (<italic>t</italic> = 2.47, <italic>p</italic> = 0.02) indicated a potential risk of publication bias, but the Trim and Fill method for sensitive analysis showed that the pooled effect size (Hedges&#x2019; <italic>g</italic> = 0.56, <italic>p</italic> &#x003C; 0.01) was robust.</p>
<fig id="F4" position="float">
<label>FIGURE 4</label>
<caption><p>Funnel plot.</p></caption>
<alt-text>Funnel plot displaying the standardized mean difference on the x-axis and the standard error on the y-axis. Dots represent individual studies, with areas of significance shaded in gray. The plot helps identify publication bias, with two p-value thresholds, less than 0.05 and less than 0.01, indicated.</alt-text>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="fnagi-17-1666449-g004.tif"/>
</fig>
<p>To examine characteristics that may contribute to the impact of Nordic walking on cognitive function, we conducted a subgroup analysis using a random effects model on studies that reported large effects involving cognitive function (<xref ref-type="table" rid="T2">Table 2</xref>). The analysis was based on the following study characteristics: participants&#x2019; health status (i.e., healthy population vs. diseased population) and control type (i.e., active vs. inactive).</p>
<table-wrap position="float" id="T2">
<label>TABLE 2</label>
<caption><p>Subgroup analysis results regarding the effects of executive function.</p></caption>
<table cellspacing="5" cellpadding="5" frame="box" rules="all">
<thead>
<tr>
<td valign="top" align="center">Variables</td>
<td valign="top" align="center">No. of studies</td>
<td valign="top" align="center">Hedges&#x2019; g (95% CI)</td>
<td valign="top" align="center"><italic>P</italic>-value</td>
<td valign="top" align="center" colspan="2">Test of heterogeneity</td>
</tr>
<tr>
<td valign="top" align="center"></td>
<td valign="top" align="center"></td>
<td valign="top" align="center"></td>
<td valign="top" align="center"></td>
<td valign="top" align="center">I<sup>2</sup> (%)</td>
<td valign="top" align="center"><italic>P</italic>-value</td>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="left" colspan="6"><bold>The health status of participants</bold></td>
</tr>
<tr>
<td valign="top" align="center">Diseased</td>
<td valign="top" align="center">2</td>
<td valign="top" align="center">0.70 (0.04, 1.35)</td>
<td valign="top" align="center">0.04<xref ref-type="table-fn" rid="t2fna"><sup>a</sup></xref></td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0.33</td>
</tr>
<tr>
<td valign="top" align="center">Healthy</td>
<td valign="top" align="center">2</td>
<td valign="top" align="center">1.06 (&#x2212;1.28, 3.40)</td>
<td valign="top" align="center">0.19</td>
<td valign="top" align="center">89.73</td>
<td valign="top" align="center">&#x003C;0.01</td>
</tr>
<tr>
<td valign="top" align="left" colspan="6"><bold>Control types</bold></td>
</tr>
<tr>
<td valign="top" align="center">Active</td>
<td valign="top" align="center">2</td>
<td valign="top" align="center">1.06 (&#x2212;1.28, 3.40)</td>
<td valign="top" align="center">0.19</td>
<td valign="top" align="center">89.73</td>
<td valign="top" align="center">&#x003C;0.01</td>
</tr>
<tr>
<td valign="top" align="center">Inactive</td>
<td valign="top" align="center">2</td>
<td valign="top" align="center">0.70 (0.04, 1.35)</td>
<td valign="top" align="center">0.04<xref ref-type="table-fn" rid="t2fna"><sup>a</sup></xref></td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0.33</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn id="t2fna"><p><italic><sup>a</sup></italic>NW group showed a statistically significant improvement (<italic>P</italic> &#x003C; 0.05).</p></fn>
</table-wrap-foot>
</table-wrap>
<p>The subgroup analysis based on participants&#x2019; health status revealed a moderate and statistically significant effect size for the disease group [Hedges&#x2019; <italic>g</italic> = 0.70, 95% CI (0.04, 1.35), <italic>p</italic> = 0.04], while the healthy group showed a large but non-significant effect size [Hedges&#x2019; <italic>g</italic> = 1.06, 95% CI (&#x2212;1.28, 3.40), <italic>p</italic> = 0.19]. The analysis by control type indicated that NW had a large but non-significant effect size compared to the active intervention control group [Hedges&#x2019; <italic>g</italic> = 1.06, 95% CI (&#x2212;1.28, 3.40), <italic>p</italic> = 0.19]. In contrast, NW showed a moderate and statistically significant effect size when compared to the inactive control group [Hedges&#x2019; <italic>g</italic> = 0.56, 95% CI (0.20, 0.92), <italic>p</italic> &#x003C; 0.01].</p>
</sec>
<sec id="S3.SS5">
<title>Sensitivity analysis</title>
<p>Of the eight included studies, one study was with a randomized crossover controlled design. To evaluate the impact of this study on the results, we performed a sensitivity analysis by excluding it. The overall effect size remained significant after excluding it [Hedges&#x2019; <italic>g</italic> = 0.49, 95% CI (0.23, 0.75), <italic>p</italic> &#x003C; 0.01], and was consistent with the previous overall effect size [Hedges&#x2019; <italic>g</italic> = 0.56, 95% CI (0.29, 0.84), <italic>p</italic> &#x003C; 0.01]. It was noted that the heterogeneity between studies assessing executive function decreased significantly after excluding this study (before exclusion: I<sup>2</sup> = 73.04%, <italic>p</italic> &#x2264; 0.01; after exclusion: I<sup>2</sup> = 8.42%, <italic>p</italic> = 0.27), suggesting the difference in the duration of the intervention contributed to the inter study heterogeneity. However, the effect size for executive function also remained significant [Hedges&#x2019; <italic>g</italic> = 0.56, 95% CI (0.06, 1.06), <italic>p</italic> = 0.04]. These results confirm the robustness of our findings. Furthermore, by eliminating one effect size at a time, we explore the robustness of the pooled results across different cognitive classifications. The sensitivity analysis showed that the results in terms of attention, executive function, information processing, memory and perception were relatively robust (<xref ref-type="supplementary-material" rid="TS1">Supplementary Figure 1</xref>). In terms of global cognition alone, after excluding a study where the control group engaged in deep-water exercise, the effect size of the meta-analysis showed a significant change. Before exclusion [Hedges&#x2019; <italic>g</italic> = 0.73, 95% CI (&#x2212;0.08, 1.53), <italic>p</italic> = 0.07], after exclusion [Hedges&#x2019; <italic>g</italic> = 0.94, 95% CI (0.23, 1.66), <italic>p</italic> = 0.02]. The above results indicate that the differences in the control conditions might be the reason for the relatively sensitive global cognitive effect. Therefore, these results should be treated with caution.</p>
</sec>
<sec id="S3.SS6">
<title>Risk of bias and GRADE</title>
<p>The methodological assessment details of each included study are presented in <xref ref-type="table" rid="T3">Table 3</xref>. The overall quality rating of all included studies is high. The overall mean score is 7.13 &#x00B1; 1.25; high-quality studies account for 100% (8/8). The level of certainty of the evidence was downgraded by one level due to limitations in study bias (<xref ref-type="supplementary-material" rid="TS1">Supplementary Table 2</xref>).</p>
<table-wrap position="float" id="T3">
<label>TABLE 3</label>
<caption><p>Quality assessment of included studies (<italic>n</italic> = 8).</p></caption>
<table cellspacing="5" cellpadding="5" frame="box" rules="all">
<thead>
<tr>
<td valign="top" align="center">References</td>
<td valign="top" align="center">Eligibility criteria</td>
<td valign="top" align="center">Random allocation</td>
<td valign="top" align="center">Concealed allocation</td>
<td valign="top" align="center">Similar baseline</td>
<td valign="top" align="center">Participant blinding</td>
<td valign="top" align="center">Investi- gator blinding</td>
<td valign="top" align="center">Assessor blinding</td>
<td valign="top" align="center">Complete- ness of follow-up</td>
<td valign="top" align="center">Intention to treat</td>
<td valign="top" align="center">Between group comparisons</td>
<td valign="top" align="center">Point measures and variability</td>
<td valign="top" align="center">Total score</td>
<td valign="top" align="center">Overall quality</td>
</tr>
</thead>
<tbody>
<tr>
<td valign="top" align="center"><xref ref-type="bibr" rid="B21">Ebersbach et al., 2014</xref></td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">7</td>
<td valign="top" align="center">High</td>
</tr>
<tr>
<td valign="top" align="center"><xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref></td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">9</td>
<td valign="top" align="center">High</td>
</tr>
<tr>
<td valign="top" align="center"><xref ref-type="bibr" rid="B30">Guszkowska et al., 2022</xref></td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">5</td>
<td valign="top" align="center">High</td>
</tr>
<tr>
<td valign="top" align="center"><xref ref-type="bibr" rid="B47">Miyazaki et al., 2022</xref></td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">8</td>
<td valign="top" align="center">High</td>
</tr>
<tr>
<td valign="top" align="center"><xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref></td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">6</td>
<td valign="top" align="center">High</td>
</tr>
<tr>
<td valign="top" align="center"><xref ref-type="bibr" rid="B38">Kettinen et al., 2023</xref></td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">7</td>
<td valign="top" align="center">High</td>
</tr>
<tr>
<td valign="top" align="center"><xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref></td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">7</td>
<td valign="top" align="center">High</td>
</tr>
<tr>
<td valign="top" align="center"><xref ref-type="bibr" rid="B31">Haas et al., 2024</xref></td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">0</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">1</td>
<td valign="top" align="center">8</td>
<td valign="top" align="center">High</td>
</tr>
</tbody>
</table></table-wrap>
</sec>
</sec>
<sec id="S4" sec-type="discussion">
<title>Discussion</title>
<p>To the best of our knowledge, this study represents the first systematic review and meta-analysis to comprehensively evaluate the effects of NW on cognitive function in older adults. The findings highlight the potential of NW as a non-pharmacological intervention to mitigate age-related cognitive decline with moderate quality of evidence. Eight studies were included, and the overall score was assessed as &#x201C;high quality&#x201D;, indicating no risk of bias. The primary results suggest that NW significantly enhances the executive function of older adults but does not substantially improve global function, memory function, attention, information processing, and perceptual ability. Meta-regression analysis revealed a positive correlation between the total duration of the intervention and the cognitive benefits. Subgroup analysis indicated that the effect of NW might be more pronounced in the diseased populations as compared to essential healthy individuals. The knowledge from this work suggests that NW should be carefully considered in future studies for the rehabilitation plans of older adults with health conditions.</p>
<p>NW showed no significant effects on cognitive domains except executive function, consistent with prior studies of standard walking (<xref ref-type="bibr" rid="B15">Chen et al., 2020</xref>a; <xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>; <xref ref-type="bibr" rid="B65">Tomoto et al., 2021</xref>; <xref ref-type="bibr" rid="B44">Lin et al., 2023</xref>). The limited cognitive benefits from walking interventions may largely reflect insufficient aerobic intensity, a well-established determinant of exercise-induced neuroplasticity (<xref ref-type="bibr" rid="B46">Mavros et al., 2017</xref>; <xref ref-type="bibr" rid="B15">Chen et al., 2020</xref>b; <xref ref-type="bibr" rid="B64">Thomas et al., 2020</xref>). Although NW increases energy expenditure compared to conventional walking, its intensity probably remains below the threshold required for broad cognitive adaptations, particularly in hippocampal-dependent memory or temporoparietal-mediated perceptual processing (<xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>; <xref ref-type="bibr" rid="B49">Nemoto et al., 2021</xref>; <xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>; <xref ref-type="bibr" rid="B38">Kettinen et al., 2023</xref>; <xref ref-type="bibr" rid="B6">Baker et al., 2025</xref>). The neurocognitive value of NW appears to stem primarily from its motor complexity rather than absolute intensity. Unlike standard walking, NW requires synchronized upper and lower limb activation through pole propulsion, recruiting supplementary motor areas, premotor cortices, and frontoparietal networks (<xref ref-type="bibr" rid="B61">Schiffer et al., 2006</xref>; <xref ref-type="bibr" rid="B22">Erickson et al., 2013</xref>; <xref ref-type="bibr" rid="B50">Niemann et al., 2014</xref>). These regions are integral to planning, decision-making, and inhibitory control (<xref ref-type="bibr" rid="B45">Liu-Ambrose et al., 2012</xref>; <xref ref-type="bibr" rid="B22">Erickson et al., 2013</xref>; <xref ref-type="bibr" rid="B69">Voelcker-Rehage and Niemann, 2013</xref>; <xref ref-type="bibr" rid="B50">Niemann et al., 2014</xref>; <xref ref-type="bibr" rid="B48">M&#x00FC;ller et al., 2017</xref>). Such enhanced cortical activation may improve synaptic density and white matter integrity, supported by elevated BDNF levels in NW interventions (<xref ref-type="bibr" rid="B27">Gmiat et al., 2018</xref>). Additionally, NW&#x2019;s rhythmic bilateral coordination may stimulate cerebellar-thalamocortical circuits critical for executive function, optimizing neural efficiency for task-switching and error monitoring (<xref ref-type="bibr" rid="B53">Passos-Monteiro et al., 2020</xref>). Consequently, NW acts as a dual-task modality that enhances prefrontal efficiency through mechanisms distinct from pure aerobic stimulation. Our meta-regression indicated longer interventions were associated with greater cognitive benefits, suggesting executive improvements emerge relatively quickly with NW, while other domains may require extended exposure to achieve intensity thresholds through cumulative neurotrophic effects (<xref ref-type="bibr" rid="B36">Jasim et al., 2024</xref>; <xref ref-type="bibr" rid="B6">Baker et al., 2025</xref>).</p>
<p>Subgroup analyses indicated greater cognitive benefits from NW in diseased populations (e.g., Alzheimer&#x2019;s patients) compared to essential healthy older adults. This difference may reflect compensatory neuroplasticity in individuals with health conditions, where baseline cognitive impairment increases sensitivity to exercise-induced neurotrophic and vascular changes (<xref ref-type="bibr" rid="B39">Kirk-Sanchez and McGough, 2013</xref>; <xref ref-type="bibr" rid="B54">Ploydang et al., 2023</xref>; <xref ref-type="bibr" rid="B37">Kadiyala et al., 2024</xref>; <xref ref-type="bibr" rid="B55">Popescu et al., 2024</xref>). For example, the capacity of NW to improve cerebral perfusion through upper limb engagement may counter hypoperfusion in Alzheimer&#x2019;s pathology, particularly benefiting clinical groups (<xref ref-type="bibr" rid="B59">Sanders et al., 2020</xref>; <xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>). Healthy older adults may require higher-intensity or cognitively enhanced NW protocols to exceed their neurocognitive reserve thresholds (<xref ref-type="bibr" rid="B70">Zhang et al., 2023</xref>). Additionally, NW demonstrated comparable efficacy to active controls (e.g., standard walking) but outperformed inactive controls. This suggests that while the biomechanical advantages of NW improve adherence and physical outcomes like reduced perceived exertion (<xref ref-type="bibr" rid="B3">Angiolillo et al., 2023</xref>; <xref ref-type="bibr" rid="B31">Haas et al., 2024</xref>), its cognitive benefits may overlap with other exercise modalities. Nevertheless, NW&#x2019;s scalability, low injury risk, and dual-task potential make it a practical option for older adults with mobility limitations. However, all studies using active controls involved essential healthy participants, making it unclear whether outcomes were influenced primarily by health status or control group type. Further research is required to clarify the relevant conclusions.</p>
<sec id="S4.SS1">
<title>Limitations</title>
<p>The results of this work still need to be taken with caution. First, the number of included studies was relatively limited (<italic>n</italic> = 8), which may potentially affect the statistical power of our meta-analysis and meta-regression results. Additionally, due to the limited number of studies, subgroup analyses to characterize the influences of protocol settings of NW (e.g., appropriate number of sessions, the frequency of intervention, and Intervention intensity) cannot be completed. Furthermore, interpretations regarding underlying mechanisms remain to be validated in future studies due to the small sample size and the limited number of existing studies. Lastly, although sensitivity analyses showed robust results, heterogeneity among the studies may have a potential influence on the interpretation of outcome measures. Nevertheless, the knowledge obtained from this work will help inform the appropriate design of intervention protocols of NW.</p>
</sec>
</sec>
<sec id="S5" sec-type="conclusion">
<title>Conclusion</title>
<p>This study suggests that NW has promise to enhance the executive function in older adults with health conditions. Future RCTs with rigorous designs are needed to help obtain more definitive conclusions on the effects of NW on cognitive function in older adults.</p>
</sec>
</body>
<back>
<sec id="S6" sec-type="data-availability">
<title>Data availability statement</title>
<p>The original contributions presented in this study are included in this article/<xref ref-type="supplementary-material" rid="TS1">Supplementary material</xref>, further inquiries can be directed to the corresponding authors.</p>
</sec>
<sec id="S7" sec-type="author-contributions">
<title>Author contributions</title>
<p>HL: Data curation, Formal analysis, Methodology, Software, Visualization, Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing. KZ: Data curation, Formal analysis, Methodology, Visualization, Writing &#x2013; original draft, Writing &#x2013; review &#x0026; editing. JG: Conceptualization, Methodology, Project administration, Visualization, Writing &#x2013; review &#x0026; editing. ZW: Conceptualization, Methodology, Visualization, Writing &#x2013; review &#x0026; editing. ZG: Methodology, Writing &#x2013; review &#x0026; editing. LL: Methodology, Writing &#x2013; review &#x0026; editing. XG: Conceptualization, Writing &#x2013; review &#x0026; editing. JN: Conceptualization, Methodology, Project administration, Supervision, Writing &#x2013; review &#x0026; editing.</p>
</sec>
<sec id="S8" sec-type="funding-information">
<title>Funding</title>
<p>The author(s) declare that no financial support was received for the research and/or publication of this article.</p>
</sec>
<sec id="S9" sec-type="COI-statement">
<title>Conflict of interest</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec id="S10" sec-type="ai-statement">
<title>Generative AI statement</title>
<p>The authors declare that no Generative AI was used in the creation of this manuscript.</p>
<p>Any alternative text (alt text) provided alongside figures in this article has been generated by Frontiers with the support of artificial intelligence and reasonable efforts have been made to ensure accuracy, including review by the authors wherever possible. If you identify any issues, please contact us.</p>
</sec>
<sec id="S11" sec-type="disclaimer">
<title>Publisher&#x2019;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
<sec id="S12" sec-type="supplementary-material">
<title>Supplementary material</title>
<p>The Supplementary Material for this article can be found online at: <ext-link ext-link-type="uri" xlink:href="https://www.frontiersin.org/articles/10.3389/fnagi.2025.1666449/full#supplementary-material">https://www.frontiersin.org/articles/10.3389/fnagi.2025.1666449/full#supplementary-material</ext-link></p>
<supplementary-material xlink:href="Table_1.docx" id="TS1" mimetype="application/vnd.openxmlformats-officedocument.wordprocessingml.document" xmlns:xlink="http://www.w3.org/1999/xlink"/>
</sec>
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